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2000:
Spreng S; Dietrich G; Niewiesk S; ter Meulen V; Gentschev I; Goebel W
Novel bacterial systems for the delivery of recombinant protein or DNA.
FEMS immunology and medical microbiology 2000;
27(
4):.
On the basis of attenuated intracellular bacteria, we have developed two delivery systems for either heterologous proteins or DNA vaccine vectors. The first system utilizes attenuated strains of Gram-negative bacteria which are engineered to secrete heterologous antigens via the alpha-hemolysin secretion system of Escherichia coli. The second system is based on attenuated suicide strains of Listeria monocytogenes, which are used for the direct delivery of eukaryotic antigen expression vectors into professional antigen presenting cells (APC) like macrophages in vitro as well as in vivo.
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