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2000Kamata Y; Tahara R; Kozaki S
Difference in hydrophobicity between botulinum type B activated and non-activated neurotoxins under low pH conditions.
Toxicon : official journal of the International Society on Toxinology 2000;38(9):1247-51.
We showed that botulinum type B activated neurotoxin with a di-chain structure became hydrophobic more quickly and extensively than did the non-activated toxin with a single-chain structure on low pH exposure. The activated toxin possessed 50-fold higher toxicity than did the non-activated type. The difference in the susceptibility to hydrophobic change may be one clue to answering the question of why the activated toxin possesses a higher toxicity than does the non-activated type.

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