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2001:
Song Q; Zirnstein G W; Swaminathan B; Gold B D
Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.
Journal of clinical microbiology 2001;
39(
5):.
Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl-0.08 M urea for 5 min at 37 degrees C resulted in reproducible isolation of H. pylori, even at low inocula (< or =10(2) CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.
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