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2002Okuno Atsuro; Hasegawa Yuriko; Nishiyama Makoto; Ohira Tsuyoshi; Ko Rinkei; Kurihara Masaaki; Matsumoto Shogo; Nagasawa Hiromichi
Preparation of an active recombinant peptide of crustacean androgenic gland hormone.
Peptides 2002;23(3):567-72.
In crustaceans, male sexual characteristics are induced by a hormone referred to as androgenic gland hormone. We have recently cloned a candidate cDNA in the terrestrial isopod Armadillidium vulgare. In order to prove that this cDNA encodes the hormone, recombinant single-chain precursor molecules consisting of B chain, C peptide and A chain were produced using both baculovirus and bacterial expression systems. Neither recombinant precursors showed activity. Digestion of only the precursor carrying a glycan moiety with lysyl endopeptidase gave a heterodimeric peptide with hormonal activity by removing a part of C peptide. These results indicate that the cDNA encodes the hormone.

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