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1992:
Scherthan H; Köhler M; Vogt P; von Malsch K; Schweizer D
Chromosomal in situ hybridization with double-labeled DNA: signal amplification at the probe level.
Cytogenetics and cell genetics 1992;
60(
1):.
A double-labeling approach was applied to nonisotopic in situ hybridization with individual cosmid and plasmid clones, using digoxigenin or biotin as label and a combination of two separate enzymatic labeling methods. Probe labeling was achieved by nick translation, followed by tailing of the probe by terminal deoxynucleotidyl transferase. The double-labeling method, in conjunction with an improved detection protocol, provides for a higher signal intensity than that obtainable with single-labeled probes.
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