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2004:
Sasahara Kyle C; Gray Michael J; Shin Sang J; Boor Kathryn J
Detection of viable Mycobacterium avium subsp. paratuberculosis using luciferase reporter systems.
Foodborne pathogens and disease 2004;
1(
4):.
Plasmid- and phage-based firefly luciferase reporter constructs were evaluated as rapid detection systems for viable Mycobacterium avium subsp. paratuberculosis (MAP). A MAP strain bearing a luciferase-encoding plasmid was detectable at 100 cells/mL in skim milk and 1000 cells/mL in whole milk. Three luciferase-encoding mycobacteriophage were evaluated for detection of wild-type MAP. The best of these, phAE85, allowed detection of >1000 cells/mL within 24-48 h. Membrane filtration did not improve the sensitivity of detection for either plasmid or phage reporters. Luciferase reporters show promise for rapid detection of viable MAP.
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