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2006:
Petrásková Lucie; Charvátová Andrea; Prikrylová Vera; Kristová Veronika; Rauvolfová Jana; Martínková Ludmila; Jiménez-Barbero Jesús; Aboitiz Nuria; Petrus Ladislav; Kren Vladimír
Preparative production and separation of 2-acetamido-2-deoxymannopyranoside-containing saccharides using borate-saturated polyolic exclusion gels.
Journal of chromatography. A 2006;
1127(
1-2):.
A new separation method based on the combination of exclusion and ion exchange chromatography in borate buffer was developed. It allows semi-preparatory and preparatory separation of isobaric N-acylhexosamines (C-2 epimers) and corresponding methyl glycosides (anomers and tautomers). Three types of polyolic gels were tested for these separations. Ion-exchange HPLC was used as a rapid and reliable method for the quantification of the respective analytes. NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions.
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