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2006:
Gessler Frank; Hampe Katrin; Schmidt Michaela; Böhnel Helge
Immunomagnetic beads assay for the detection of botulinum neurotoxin types C and D.
Diagnostic microbiology and infectious disease 2006;
56(
3):.
An immunomagnetic beads assay for the simultaneous quantification of botulinum neurotoxin types C and D was developed. Specific monoclonal antibodies against the heavy chain of the toxin and affinity-purified biotinylated polyclonal antibodies (pAbs) were used. The antibodies were preincubated with the sample. The complex being formed was then captured by magnetic beads coated with antimouse IgG. Streptavidin-poly-horseradish peroxidase, a signal amplifier, bound to the biotinylated pAb. A maximum sensitivity of approximately 0.3 minimal lethal doses for mice per milliliter was achieved with culture supernatants of both toxin types.
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