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2006:
Janowski Robert; Nasser Eddine Ali; Kaufmann Stefan H E; Weiss Manfred S
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv2827c from Mycobacterium tuberculosis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;
62(
Pt 8):.
The hypothetical protein Rv2827c from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. It was purified using affinity and size-exclusion chromatographic techniques and then crystallized. Preliminary X-ray diffraction data analysis suggests the presence of two translationally related molecules in the asymmetric unit of the orthorhombic crystals.
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