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2007:
Riedel Christian U; Monk Ian R; Casey Pat G; Morrissey David; O'Sullivan Gerald C; Tangney Mark; Hill Colin; Gahan Cormac G M
Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.
Applied and environmental microbiology 2007;
73(
9):.
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.
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