Preview
Sign-in for full Details 
Sign-in free and Explore the Exciting World of BiomedExperts:
- Over 1.500.000 Profiles
- More than 1.800 Organizations worldwide
- State of the Art Network Visualizations
- Manage your own Profile
- Locate Experts in your Country/Region
- Locate Experts in your 1. and 2. Level Network
- Connect to Experts Worldwide
find experts for
Sign-in to see more
2007:
Bocca Beatrice; Lamazza Antonella; Pino Anna; De Masi Ercole; Iacomino Maura; Mattei Daniela; Rahimi Siavash; Fiori Enrico; Schillaci Alberto; Alimonti Alessandro; Forte Giovanni
Determination of 30 elements in colorectal biopsies by sector field inductively coupled plasma mass spectrometry: method development and preliminary baseline levels.
Rapid communications in mass spectrometry : RCM 2007;
21(
11):.
An analytical procedure applicable to restricted sample sizes was developed and applied to the analysis of 30 chemical elements in colorectal biopsies of healthy patients. Acidic microwave digestion processed </=10 mg of tissue at 80 degrees C in 15-mL polystyrene liners. The digests were diluted to a volume of 2 mL with high-purity water and directly analyzed by sector field inductively coupled plasma mass spectrometry without further specimen handling. A careful selection of isotopes and instrumental resolution permitted the quantification in a single analytical sequence both of the elements present at parts per trillion and of those at parts per million. The accuracy calculated on BCR 184 ranged from 93.3-110%, the recoveries of the biopsy material was in the range 95.2-105%, the precision was <10%, and the blank levels were much below those expected in biopsy samples. The metal concentrations (on a dry-weight basis) in colorectal normal tissue showed a large range of variation: Ag, Au, Be, Bi, Co, Li, Sb, Tl, V, W and Zr were below 50 ng g(-1); As, Ba, Cd, Cr, Cs, Hg, Mo, Ni, Pb, Se and Sn were distributed from 100 to 500 ng g(-1); Al, Cu, Fe, Mn, Sr and Zn were from a few microg g(-1) to 100 microg g(-1); and Ca and Mg were at a level of 1000 microg g(-1). These data represent the first attempt to achieve an elemental profile in the colorectal mucosa of healthy patients as baseline level measurements for studies focused on the imbalance of chemical elements in diseased mucosa.
Post to CiteULike 
Sign in free and see...
Visualized networks:
See your personal network in
sophisticated graphical views
GeoTargeted Searches:
Locate experts around the world
and connect with global collaborators
Research Profiles:
See the visualized research activity
of experts around the globe
Sign-in to see more
