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2007:
Sarkar Devanand; Kang Dong-chul; Fisher Paul B
Reciprocal subtraction differential RNA display (RSDD): an efficient technology for cloning differentially expressed genes.
Methods in molecular biology (Clifton, N.J.) 2007;
383(
):.
Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely and successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and limitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential RNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes.
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