Preview
Sign-in for full Details 
Sign-in free and Explore the Exciting World of BiomedExperts:
- Over 1,800,000 Profiles
- More than 3,500 Organizations worldwide
- State of the Art Network Visualizations
- Manage your own Profile
- Locate Experts in your Country/Region
- Locate Experts in your 1. and 2. Level Network
- Connect to Experts Worldwide
find experts for
Sign-in to see more
2007:
Carraway Hetty; Herman James
Monitoring methylation and gene expression in cancer.
Methods in molecular biology (Clifton, N.J.) 2007;
383(
):.
Identification of patterns of DNA methylation in higher order eukaryotes has become necessary through recognition that disease processes such as cancer can result from abnormal methylation patterns. Abnormal DNA methylation affecting the promoter region of genes can halt their expression, making DNA methylation a marker of gene inactivation. The explosion of studies involving changes in DNA methylation in the last 10 yr, particularly in the study of cancer, are largely the result of two factors: the increasing awareness of the importance of epigenetic silencing in cancer and the improvements in the techniques used to determine changes in DNA methylation. In this chapter, focus is made on the specific methods of how to perform bisulfite modification of DNA and polymerase chain reaction as well as nested methylation specific polymerase chain reaction, and discuss other techniques to evaluate DNA methylation along with the limitations inherent in each process.
Post to CiteULike 
Sign in free and see...
Visualized networks:
See your personal network in
sophisticated graphical views
GeoTargeted Searches:
Locate experts around the world
and connect with global collaborators
Research Profiles:
See the visualized research activity
of experts around the globe
Sign-in to see more
