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2007:
Sikutová Silvie; Rudolf Ivo; Golovchenko Maryna; Rudenko Nataliia; Grubhoffer Libor; Hubálek Zdenek
Detection of Anaplasma dna in Ixodes ricinus ticks: pitfalls.
Folia parasitologica 2007;
54(
4):.
A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to "Candidatus Ehrlichia walkefii". The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks.
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