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2008:
Benson Robert; Tondella Maria L; Bhatnagar Julu; Carvalho Maria da Glória S; Sampson Jacquelyn S; Talkington Deborah F; Whitney Anne M; Mothershed Elizabeth; McGee Lesley; Carlone George; McClee Vondguraus; Guarner Jeannette; Zaki Sherif; Dejsiri Surang; Cronin K; Han Jian; Fields Barry S
Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.
Journal of clinical microbiology 2008;
46(
6):.
The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.
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