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2009: Dotti Gianpietro; Tian Mei; Savoldo Barbara; Najjar Amer; Cooper Laurence J N; Jackson James; Smith Amanda; Mawlawi Osama; Uthamanthil Rajesh; Borne Agatha; Brammer David; Paolillo Vincenzo; Alauddin Mian; Gonzalez Carlos; Steiner David; Decker William K; Marini Frank; Kornblau Steven; Bollard Catherine M; Shpall Elizabeth J; Gelovani Juri G
Repetitive noninvasive monitoring of HSV1-tk-expressing T cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU.
Molecular imaging : official journal of the Society for Molecular Imaging 2009;8(4):230-7.
Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs remains largely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [18F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil (18F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.

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