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2009:
Salzameda Nicholas T; Barbieri Joseph T; Janda Kim D
Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors.
Bioorganic & medicinal chemistry letters 2009;
19(
20):.
A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition.
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