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1977:
Yolken R H; Greenberg H B; Merson M H; Sack R B; Kapikian A Z
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Journal of clinical microbiology 1977;
6(
5):.
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
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