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1996:
Tuchili L M; Kodama H; Sharma R N; Takatori I; Pandey G S; Kabilika S; Mukamoto M; Tsuji S; Baba T
Detection of Salmonella DNA in chicken embryos and environmental samples by polymerase chain reaction.
The Journal of veterinary medical science / the Japanese Society of Veterinary Science 1996;
58(
9):.
By polymerase chain reaction (PCR) using a pair of primers specific for Salmonella phoE gene a 365-bp specific gene fragment could be amplified from yolk of infertile eggs and dead-in-shell chicken embryos, and from environmental samples. Out of 45 dead-in-shell embryo samples, 20 (44.4%) were found positive for Salmonella DNA by PCR compared to 11 (24.4%) by bacteria isolation. Salmonella DNA could also be detected from infertile eggs, chicken faeces, floor litter and chick fluff, which incidence was higher than that by bacteria isolation.
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