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David Agard

Research Profile

Concepts & Ideas

Chemicals & Drugs

Physiology

Protein Folding

Anatomy

Activities & Behaviors

Image Processing, Computer-Assisted

Procedures

Genes & Molecular Sequences

Amino Acid Sequences

Occupations

Thermodynamics

Co-author Network

Publications

TOP 3
Ertan Ozyamak; David A Agard; Justin Kollman; Arash Komeili
The bacterial actin MamK: in vitro assembly behavior and filament architecture.
Dina Schneidman-Duhovny; Solmaz Sobhanifar; Pavel Strop; Javier Velázquez-Muriel; David A Agard; Agustin Avila-Sakar; Yifan Cheng; Volker Dötsch; Ho Min Kim; Seung Joong Kim; Kristin A Krukenberg; Hong Liang; Maofu Liao; Jaume Pons; Arvind Rajpal; Andrea Rossi; Andrej Sali
A method for integrative structure determination of protein-protein complexes.
Vito Mennella; Gregory Rogers; B Chhun; B Huang; F Kan; B Keszthelyi; K L McDonald; David A Agard
Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization.

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All Publications

2013: Ertan Ozyamak; David A Agard; Justin Kollman; Arash Komeili
The bacterial actin MamK: in vitro assembly behavior and filament architecture.
The Journal of biological chemistry 2013;288(6):4265-77.
2012: Dina Schneidman-Duhovny; Solmaz Sobhanifar; Pavel Strop; Javier Velázquez-Muriel; David A Agard; Agustin Avila-Sakar; Yifan Cheng; Volker Dötsch; Ho Min Kim; Seung Joong Kim; Kristin A Krukenberg; Hong Liang; Maofu Liao; Jaume Pons; Arvind Rajpal; Andrea Rossi; Andrej Sali
A method for integrative structure determination of protein-protein complexes.
Bioinformatics (Oxford, England) 2012;28(24):3282-9.
2012: Vito Mennella; Gregory Rogers; B Chhun; B Huang; F Kan; B Keszthelyi; K L McDonald; David A Agard
Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization.
Nature cell biology 2012;14(11):1159-68.
2012: Christian N Cunningham; Kristin A Krukenberg; Daniel Ryland Southworth; David A Agard
The conserved arginine 380 of Hsp90 is not a catalytic residue, but stabilizes the closed conformation required for ATP hydrolysis.
Protein science : a publication of the Protein Society 2012;21(8):1162-71.
2012: James A Kraemer; Elizabeth A Montabana; Katrina Nguyen; Duy Stephen L Pham; David A Agard; Marcella L Erb; Christopher A Waddling; Hannah Wang; Elena A Zehr; Joe Pogliano
A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell.
Cell 2012;149(7):1488-99.
2012: Brian A Kelch; Neema L Salimi; David A Agard
Functional modulation of a protein folding landscape via side-chain distortion.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(24):9414-9.
2012: L Shao; L Winoto; David A Agard; M G L Gustafsson; John W Sedat
Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras.
Journal of microscopy 2012;246(3):229-36.
2012: Muthuvel Arigovindan; John W Sedat; David A Agard
Effect of depth dependent spherical aberrations in 3D structured illumination microscopy.
Optics express 2012;20(6):6527-41.
2012: Sam Li; Wallace F Marshall; Jose-Jesus Fernandez; David A Agard
Three-dimensional structure of basal body triplet revealed by electron cryo-tomography.
The EMBO journal 2012;31(3):552-62.
2011: Justin M Kollman; Andreas Merdes; Lionel Mourey; David A Agard
Microtubule nucleation by γ-tubulin complexes.
Nature reviews. Molecular cell biology 2011;12(11):709-21.
2011: Shawn Q Zheng; Eric Branlund; Michael B Braunfeld; Yifan Cheng; Bettina Kesthelyi; John W Sedat; David A Agard
A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction.
Ultramicroscopy 2011;111(8):1137-43.
2011: Daniel Ryland Southworth; David A Agard
Client-loading conformation of the Hsp90 molecular chaperone revealed in the cryo-EM structure of the human Hsp90:Hop complex.
Molecular cell 2011;42(6):771-81.
2011: Kristin A Krukenberg; Timothy O Street; Laura A Lavery; David A Agard
Conformational dynamics of the molecular chaperone Hsp90.
Quarterly reviews of biophysics 2011;44(2):229-55.
2011: Christopher R Rivera; Justin M Kollman; Jessica K Polka; David A Agard; Dyche R D Mullins
Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.
The Journal of biological chemistry 2011;286(16):14282-90.
2011: Timothy O Street; Laura A Lavery; David A Agard
Substrate binding drives large-scale conformational changes in the Hsp90 molecular chaperone.
Molecular cell 2011;42(1):96-105.
2010: Peter Carlton; Jérôme Boulanger; Charles Kervrann; Jean-Baptiste Sibarita; Jean Salamero; Susannah Gordon-Messer; Debra Bressan; James E Haber; Sebastian Haase; Lin Shao; Lukman Winoto; Atsushi Matsuda; Peter Kner; Satoru Uzawa; Mats Gustafsson; Zvi Kam; David A Agard; John W Sedat
Fast live simultaneous multiwavelength four-dimensional optical microscopy.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(37):16016-22.
2010: Justin M Kollman; Jessica K Polka; Alex Zelter; Trisha N Davis; David A Agard
Microtubule nucleating gamma-TuSC assembles structures with 13-fold microtubule-like symmetry.
Nature 2010;466(7308):879-82.
2010: Muthuvel Arigovindan; Joshua Shaevitz; John McGowan; John W Sedat; David A Agard
A Parallel Product-Convolution approach for representing the depth varying Point Spread Functions in 3D widefield microscopy based on principal component analysis.
Optics express 2010;18(7):6461-76.
2010: Bosco K Ho; David A Agard
Conserved tertiary couplings stabilize elements in the PDZ fold, leading to characteristic patterns of domain conformational flexibility.
Protein science : a publication of the Protein Society 2010;19(3):398-411.
2010: Neema L Salimi; Bosco Ho; David A Agard
Unfolding simulations reveal the mechanism of extreme unfolding cooperativity in the kinetically stable alpha-lytic protease.
PLoS computational biology 2010;6(2):e1000689.
2010: P Kner; John W Sedat; David A Agard; Z Kam
High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing.
Journal of microscopy 2010;237(2):136-47.
2010: Bosco K Ho; David A Agard
An improved strategy for generating forces in steered molecular dynamics: the mechanical unfolding of titin, e2lip3 and ubiquitin.
PloS one 2010;5(9):.
2010: Shawn Q Zheng; John W Sedat; David A Agard
Automated data collection for electron microscopic tomography.
Methods in enzymology 2010;481():283-315.
2010: Atsushi Matsuda; Lin Shao; Jerome Boulanger; Charles Kervrann; Peter Carlton; Peter Kner; David A Agard; John W Sedat
Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones.
PloS one 2010;5(9):e12768.
2010: Timothy O Street; Kristin A Krukenberg; Jörg Rosgen; D Wayne Bolen; David A Agard
Osmolyte-induced conformational changes in the Hsp90 molecular chaperone.
Protein science : a publication of the Protein Society 2010;19(1):57-65.
2009: Rebeca M Choy; Justin M Kollman; Alex Zelter; Trisha Davis; David A Agard
Localization and orientation of the gamma-tubulin small complex components using protein tags as labels for single particle EM.
Journal of structural biology 2009;168(3):571-4.
2009: Shawn Q Zheng; Atsushi Matsuda; Michael B Braunfeld; John W Sedat; David A Agard
Dual-axis target mapping and automated sequential acquisition of dual-axis EM tomographic data.
Journal of structural biology 2009;168(2):323-31.
2009: Jessica K Polka; Justin M Kollman; David A Agard; Dyche R D Mullins
The structure and assembly dynamics of plasmid actin AlfA imply a novel mechanism of DNA segregation.
Journal of bacteriology 2009;191(20):6219-30.
2009: Kristin A Krukenberg; Ulrike M K Böttcher; Daniel Ryland Southworth; David A Agard
Grp94, the endoplasmic reticulum Hsp90, has a similar solution conformation to cytosolic Hsp90 in the absence of nucleotide.
Protein science : a publication of the Protein Society 2009;18(9):1815-27.
2009: Kristin A Krukenberg; Daniel Ryland Southworth; Timothy O Street; David A Agard
pH-dependent conformational changes in bacterial Hsp90 reveal a Grp94-like conformation at pH 6 that is highly active in suppression of citrate synthase aggregation.
Journal of molecular biology 2009;390(2):278-91.
2009: Bosco K Ho; David A Agard
Probing the flexibility of large conformational changes in protein structures through local perturbations.
PLoS computational biology 2009;5(4):e1000343.
2008: Daniel Ryland Southworth; David A Agard
Species-dependent ensembles of conserved conformational states define the Hsp90 chaperone ATPase cycle.
Molecular cell 2008;32(5):631-40.
2008: Bengt Nölting; David A Agard
How general is the nucleation-condensation mechanism?
Proteins 2008;73(3):754-64.
2008: Friedrich Förster; Benjamin Webb; Kristin A Krukenberg; Hiro Tsuruta; David A Agard; Andrej Sali
Integration of small-angle X-ray scattering data into structural modeling of proteins and their assemblies.
Journal of molecular biology 2008;382(4):1089-106.
2008: Christian N Cunningham; Kristin A Krukenberg; David A Agard
Intra- and intermonomer interactions are required to synergistically facilitate ATP hydrolysis in Hsp90.
The Journal of biological chemistry 2008;283(30):21170-8.
2008: Lothar Schermelleh; Peter Carlton; Sebastian Haase; Lin Shao; Lukman Winoto; Peter Kner; Brian Burke; M Cristina Cardoso; David A Agard; Mats G L Gustafsson; Heinrich Leonhardt; John W Sedat
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.
Science (New York, N.Y.) 2008;320(5881):1332-6.
2008: Mats G L Gustafsson; Lin Shao; Peter Carlton; CHUNG-JU RACHEL WANG; Inna N Golubovskaya; W Zacheus Cande; David A Agard; John W Sedat
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Biophysical journal 2008;94(12):4957-70.
2008: Lin Shao; Berith Isaac; Satoru Uzawa; David A Agard; John W Sedat; Mats G L Gustafsson
I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.
Biophysical journal 2008;94(12):4971-83.
2008: Kristin A Krukenberg; Friedrich Förster; Luke M Rice; Andrej Sali; David A Agard
Multiple conformations of E. coli Hsp90 in solution: insights into the conformational dynamics of Hsp90.
Structure (London, England : 1993) 2008;16(5):755-65.
2008: Matthew A Trammell; Nicole Mahoney; David A Agard; Ronald D Vale
Mob4 plays a role in spindle focusing in Drosophila S2 cells.
Journal of cell science 2008;121(Pt 8):1284-92.
2008: Luke M Rice; Elizabeth A Montabana; David A Agard
The lattice as allosteric effector: structural studies of alphabeta- and gamma-tubulin clarify the role of GTP in microtubule assembly.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(14):5378-83.
2008: Bosco K Ho; David A Agard
Identification of new, well-populated amino-acid sidechain rotamers involving hydroxyl-hydrogen atoms and sulfhydryl-hydrogen atoms.
BMC structural biology 2008;8():41.
2008: Justin M Kollman; Alex Zelter; Eric G D Muller; Bethany Fox; Luke M Rice; Trisha Davis; David A Agard
The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation.
Molecular biology of the cell 2008;19(1):207-15.
2007: Peter König; Michael B Braunfeld; John W Sedat; David A Agard
The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.
Chromosoma 2007;116(4):349-72.
2007: Brian A Kelch; David A Agard
Mesophile versus thermophile: insights into the structural mechanisms of kinetic stability.
Journal of molecular biology 2007;370(4):784-95.
2007: Erik F Y Hom; Franck Marchis; Timothy K Lee; Sebastian Haase; David A Agard; John W Sedat
AIDA: an adaptive image deconvolution algorithm with application to multi-frame and three-dimensional data.
Journal of the Optical Society of America. A, Optics, image science, and vision 2007;24(6):1580-600.
2007: Brian A Kelch; Kyle P Eagen; F Pinar Erciyas; Elisabeth L Humphris; Adam R Thomason; Shinji Mitsuiki; David A Agard
Structural and mechanistic exploration of acid resistance: kinetic stability facilitates evolution of extremophilic behavior.
Journal of molecular biology 2007;368(3):870-83.
2007: Zvi Kam; Peter Kner; David A Agard; John W Sedat
Modelling the application of adaptive optics to wide-field microscope live imaging.
Journal of microscopy 2007;226(Pt 1):33-42.
2007: Shawn Q Zheng; Justin M Kollman; Michael B Braunfeld; John W Sedat; David A Agard
Automated acquisition of electron microscopic random conical tilt sets.
Journal of structural biology 2007;157(1):148-55.
2007: Shawn Q Zheng; Bettina Keszthelyi; Eric Branlund; John M Lyle; Michael B Braunfeld; John W Sedat; David A Agard
UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.
Journal of structural biology 2007;157(1):138-47.
2006: Koji Yonekura; Michael B Braunfeld; Saori Maki-Yonekura; David A Agard
Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV.
Journal of structural biology 2006;156(3):524-36.
2006: Andrew K Shiau; Seth F Harris; Daniel Ryland Southworth; David A Agard
Structural Analysis of E. coli hsp90 reveals dramatic nucleotide-dependent conformational rearrangements.
Cell 2006;127(2):329-40.
2006: Bin Wei; Peter G Lam; Michael B Braunfeld; David A Agard; Jan Genzer; Richard J Spontak
Tunable instability mechanisms of polymer thin films by molecular self-assembly.
Langmuir : the ACS journal of surfaces and colloids 2006;22(21):8642-5.
2006: Cynthia N Fuhrmann; Matthew D Daugherty; David A Agard
Subangstrom crystallography reveals that short ionic hydrogen bonds, and not a His-Asp low-barrier hydrogen bond, stabilize the transition state in serine protease catalysis.
Journal of the American Chemical Society 2006;128(28):9086-102.
2005: Stephanie M E Truhlar; David A Agard
The folding landscape of an alpha-lytic protease variant reveals the role of a conserved beta-hairpin in the development of kinetic stability.
Proteins 2005;61(1):105-14.
2005: Nobuyuki Ota; David A Agard
Intramolecular signaling pathways revealed by modeling anisotropic thermal diffusion.
Journal of molecular biology 2005;351(2):345-54.
2005: W C Moss; S Haase; J M Lyle; David A Agard; John W Sedat
A novel 3D wavelet-based filter for visualizing features in noisy biological data.
Journal of microscopy 2005;219(Pt 2):43-9.
2005: Peter König; Michael B Braunfeld; David A Agard
Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography.
Ultramicroscopy 2005;103(4):261-74.
2005: Hector Aldaz; Luke M Rice; Tim Stearns; David A Agard
Insights into microtubule nucleation from the crystal structure of human gamma-tubulin.
Nature 2005;435(7041):523-7.
2005: Sheila S Jaswal; Stephanie M E Truhlar; Ken A Dill; David A Agard
Comprehensive analysis of protein folding activation thermodynamics reveals a universal behavior violated by kinetically stable proteases.
Journal of molecular biology 2005;347(2):355-66.
2004: Satoru Uzawa; Fei Li; Ye Jin; Kent L McDonald; Michael B Braunfeld; David A Agard; W Zacheus Cande
Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+.
Molecular biology of the cell 2004;15(12):5219-30.
2004: Alastair A MacDowell; Richard S Celestre; Malcolm Howells; Wayne R Mckinney; James Krupnick; Daniella Cambie; Edward E Domning; Robert M Duarte; Nicholas Kelez; David W Plate; Carl Cork; Thomas N Earnest; Jeffery Dickert; George Meigs; Corie Ralston; James M Holton; Thomas Alber; James M Berger; David A Agard; Howard A Padmore
Suite of three protein crystallography beamlines with single superconducting bend magnet as the source.
Journal of synchrotron radiation 2004;11(Pt 6):447-55.
2004: Bridget M Hanser; Mats M G L Gustafsson; David A Agard; John W Sedat
Phase-retrieved pupil functions in wide-field fluorescence microscopy.
Journal of microscopy 2004;216(Pt 1):32-48.
2004: Qingxiong S Zheng; Michael B Braunfeld; John W Sedat; David A Agard
An improved strategy for automated electron microscopic tomography.
Journal of structural biology 2004;147(2):91-101.
2004: Seth F Harris; Andrew K Shiau; David A Agard
The crystal structure of the carboxy-terminal dimerization domain of htpG, the Escherichia coli Hsp90, reveals a potential substrate binding site.
Structure (London, England : 1993) 2004;12(6):1087-97.
2004: Cynthia N Fuhrmann; Brian A Kelch; Nobuyuki Ota; David A Agard
The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain.
Journal of molecular biology 2004;338(5):999-1013.
2004: Erin L Cunningham; David A Agard
Disabling the folding catalyst is the last critical step in alpha-lytic protease folding.
Protein science : a publication of the Protein Society 2004;13(2):325-31.
2004: Stephanie M E Truhlar; Erin L Cunningham; David A Agard
The folding landscape of Streptomyces griseus protease B reveals the energetic costs and benefits associated with evolving kinetic stability.
Protein science : a publication of the Protein Society 2004;13(2):381-90.
2003: Erin L Cunningham; David A Agard
Interdependent folding of the N- and C-terminal domains defines the cooperative folding of alpha-lytic protease.
Biochemistry 2003;42(45):13212-9.
2003: Bridget M Hanser; Mats M G L Gustafsson; David A Agard; John W Sedat
Phase retrieval for high-numerical-aperture optical systems.
Optics letters 2003;28(10):801-3.
2003: Irwin D Kuntz; David A Agard
Assessment of the role of computations in structural biology.
Advances in protein chemistry 2003;66():1-25.
2002: Chih-Ying Chen; Michael Reese; Peter K Hwang; Nobuyuki Ota; David A Agard; Frances M Brodsky
Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans.
The EMBO journal 2002;21(22):6072-82.
2002: Luke M Rice; David A Agard
Centriole duplication: centrin in on answers?
Current biology : CB 2002;12(18):R618-9.
2002: Erin L Cunningham; Ted Mau; Stephanie M E Truhlar; David A Agard
The pro region N-terminal domain provides specific interactions required for catalysis of alpha-lytic protease folding.
Biochemistry 2002;41(28):8860-7.
2002: Andrew K Shiau; Danielle Barstad; James T Radek; Marvin J Meyers; Kendall W. Nettles; Benita S Katzenellenbogen; John A Katzenellenbogen; David A Agard; Geoffrey L Greene
Structural characterization of a subtype-selective ligand reveals a novel mode of estrogen receptor antagonism.
Nature structural biology 2002;9(5):359-64.
2002: Holger Wille; Melissa D Michelitsch; Vincent Guenebaut; Surachai Supattapone; Ana Serban; Fred Cohen; David A Agard; Stanley B Prusiner
Structural studies of the scrapie prion protein by electron crystallography.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(6):3563-8.
2002: Sheila S Jaswal; Julie L Sohl; Jonathan H Davis; David A Agard
Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability.
Nature 2002;415(6869):343-6.
2001: Nobuyuki Ota; David A Agard
Binding mode prediction for a flexible ligand in a flexible pocket using multi-conformation simulated annealing pseudo crystallographic refinement.
Journal of molecular biology 2001;314(3):607-17.
2001: S M Murphy; Andrea M Preble; U K Patel; K L O'Connell; D P Dias; M Moritz; David A Agard; John T Stults; Tim Stearns
GCP5 and GCP6: two new members of the human gamma-tubulin complex.
Molecular biology of the cell 2001;12(11):3340-52.
2001: Nobuyuki Ota; David A Agard
Enzyme specificity under dynamic control II: Principal component analysis of alpha-lytic protease using global and local solvent boundary conditions.
Protein science : a publication of the Protein Society 2001;10(7):1403-14.
2001: wallace marshall; J F Marko; David A Agard; John W Sedat
Chromosome elasticity and mitotic polar ejection force measured in living Drosophila embryos by four-dimensional microscopy-based motion analysis.
Current biology : CB 2001;11(8):569-78.
2001: M Moritz; David A Agard
Gamma-tubulin complexes and microtubule nucleation.
Current opinion in structural biology 2001;11(2):174-81.
2001: Zvi Kam; Bridget M Hanser; Mats M G L Gustafsson; David A Agard; John W Sedat
Computational adaptive optics for live three-dimensional biological imaging.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(7):3790-5.
2001: M Moritz; Michael B Braunfeld; Bruce Alberts; David A Agard
Reconstitution of centrosome microtubule nucleation in Drosophila.
Methods in cell biology 2001;67():141-8.
2000: M Moritz; Michael B Braunfeld; V Guénebaut; John Edward Heuser; David A Agard
Structure of the gamma-tubulin ring complex: a template for microtubule nucleation.
Nature cell biology 2000;2(6):365-70.
2000: A I Derman; David A Agard
Two energetically disparate folding pathways of alpha-lytic protease share a single transition state.
Nature structural biology 2000;7(5):394-7.
2000: Hank Bass; Oscar Riera-Lizarazu; Evgueni V Ananiev; Stefano J Bordoli; Howard W Rines; Ronald L Phillips; John W Sedat; David A Agard; W Zacheus Cande
Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase.
Journal of cell science 2000;113 ( Pt 6)():1033-42.
2000: Hiroshi Jinnai; Yukihiro Nishikawa; Richard J Spontak; Steven D Smith; David A Agard; Takeji Hashimoto
Direct measurement of interfacial curvature distributions in a bicontinuous block copolymer morphology.
Physical review letters 2000;84(3):518-21.
2000: Peter J Kushner; David A Agard; W J Feng; G Lopez; A Schiau; Rosalie M Uht; Paul Webb; G Greene
Oestrogen receptor function at classical and alternative response elements.
Novartis Foundation symposium 2000;230():20-6; discussion 27-40.
1999: Erin L Cunningham; Sheila S Jaswal; Julie L Sohl; David A Agard
Kinetic stability as a mechanism for protease longevity.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(20):11008-14.
1999: Mats M G L Gustafsson; David A Agard; John W Sedat
I5M: 3D widefield light microscopy with better than 100 nm axial resolution.
Journal of microscopy 1999;195(Pt 1):10-6.
1999: D E Anderson; R J Peters; B Wilk; David A Agard
alpha-lytic protease precursor: characterization of a structured folding intermediate.
Biochemistry 1999;38(15):4728-35.
1999: D W Miller; David A Agard
Enzyme specificity under dynamic control: a normal mode analysis of alpha-lytic protease.
Journal of molecular biology 1999;286(1):267-78.
1998: Andrew K Shiau; D Barstad; P M Loria; L Cheng; Peter J Kushner; David A Agard; Geoffrey L Greene
The structural basis of estrogen receptor/coactivator recognition and the antagonism of this interaction by tamoxifen.
Cell 1998;95(7):927-37.
1998: N K Sauter; Ted Mau; Stephen D Rader; David A Agard
Structure of alpha-lytic protease complexed with its pro region.
Nature structural biology 1998;5(11):945-50.
1998: Julie L Sohl; Sheila S Jaswal; David A Agard
Unfolded conformations of alpha-lytic protease are more stable than its native state.
Nature 1998;395(6704):817-9.
1998: R J Peters; A K Shiau; Julie L Sohl; D E Anderson; G Tang; J L Silen; David A Agard
Pro region C-terminus:protease active site interactions are critical in catalyzing the folding of alpha-lytic protease.
Biochemistry 1998;37(35):12058-67.
1998: J H Davis; David A Agard
Relationship between enzyme specificity and the backbone dynamics of free and inhibited alpha-lytic protease.
Biochemistry 1998;37(21):7696-707.
1998: Jennifer Fung; wallace marshall; Abby F Dernburg; David A Agard; John W Sedat
Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations.
The Journal of cell biology 1998;141(1):5-20.
1997: K F Han; John W Sedat; David A Agard
Practical image restoration of thick biological specimens using multiple focus levels in transmission electron microscopy.
Journal of structural biology 1997;120(3):237-44.
1997: wallace marshall; Aaron F Straight; J F Marko; J Swedlow; Abby F Dernburg; Andrew S Belmont; Andrew W Murray; David A Agard; John W Sedat
Interphase chromosomes undergo constrained diffusional motion in living cells.
Current biology : CB 1997;7(12):930-9.
1997: J H Davis; David A Agard; T M Handel; Vladimir J Basus
Alterations in chemical shifts and exchange broadening upon peptide boronic acid inhibitor binding to alpha-lytic protease.
Journal of biomolecular NMR 1997;10(1):21-7.
1997: Stephen D Rader; David A Agard
Conformational substates in enzyme mechanism: the 120 K structure of alpha-lytic protease at 1.5 A resolution.
Protein science : a publication of the Protein Society 1997;6(7):1375-86.
1997: Hank Bass; wallace marshall; John W Sedat; David A Agard; W Zacheus Cande
Telomeres cluster de novo before the initiation of synapsis: a three-dimensional spatial analysis of telomere positions before and during meiotic prophase.
The Journal of cell biology 1997;137(1):5-18.
1997: Julie L Sohl; A K Shiau; Stephen D Rader; B J Wilk; David A Agard
Inhibition of alpha-lytic protease by pro region C-terminal steric occlusion of the active site.
Biochemistry 1997;36(13):3894-902.
1996: K F Han; A J Gubbens; John W Sedat; David A Agard
Optimal strategies for imaging thick biological specimens: exit wavefront reconstruction and energy-filtered imaging.
Journal of microscopy 1996;183(Pt 2):124-32.
1996: Abby F Dernburg; Karl W Broman; Jennifer Fung; wallace marshall; J Philips; David A Agard; John W Sedat
Perturbation of nuclear architecture by long-distance chromosome interactions.
Cell 1996;85(5):745-59.
1996: wallace marshall; Abby F Dernburg; B Harmon; David A Agard; John W Sedat
Specific interactions of chromatin with the nuclear envelope: positional determination within the nucleus in Drosophila melanogaster.
Molecular biology of the cell 1996;7(5):825-42.
1996: B A Scalettar; J R Swedlow; John W Sedat; David A Agard
Dispersion, aberration and deconvolution in multi-wavelength fluorescence images.
Journal of microscopy 1996;182(Pt 1):50-60.
1996: Michael R Paddy; H Saumweber; David A Agard; John W Sedat
Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos.
Journal of cell science 1996;109 ( Pt 3)():591-607.
1996: Jennifer Fung; W Liu; W J de Ruijter; H Chen; C K Abbey; John W Sedat; David A Agard
Toward fully automated high-resolution electron tomography.
Journal of structural biology 1996;116(1):181-9.
1996: H Chen; D D Hughes; T A Chan; John W Sedat; David A Agard
IVE (Image Visualization Environment): a software platform for all three-dimensional microscopy applications.
Journal of structural biology 1996;116(1):56-60.
1995: J E Mace; David A Agard
Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity.
Journal of molecular biology 1995;254(4):720-36.
1995: M Moritz; M B Braunfeld; John W Sedat; Bruce Alberts; David A Agard
Microtubule nucleation by gamma-tubulin-containing rings in the centrosome.
Nature 1995;378(6557):638-40.
1995: Y Urata; S J Parmelee; David A Agard; John W Sedat
A three-dimensional structural dissection of Drosophila polytene chromosomes.
The Journal of cell biology 1995;131(2):279-95.
1995: M Moritz; M B Braunfeld; Jennifer Fung; John W Sedat; Bruce Alberts; David A Agard
Three-dimensional structural characterization of centrosomes from early Drosophila embryos.
The Journal of cell biology 1995;130(5):1149-59.
1995: J E Mace; B J Wilk; David A Agard
Functional linkage between the active site of alpha-lytic protease and distant regions of structure: scanning alanine mutagenesis of a surface loop affects activity and substrate specificity.
Journal of molecular biology 1995;251(1):116-34.
1995: K F Han; John W Sedat; David A Agard
Mechanism of image formation for thick biological specimens: exit wavefront reconstruction and electron energy-loss spectroscopic imaging.
Journal of microscopy 1995;178(Pt 2):107-19.
1994: L M Dong; C Wilson; Mark Wardell; T Simmons; Robert W Mahley; Karl H Weisgraber; David A Agard
Human apolipoprotein E. Role of arginine 61 in mediating the lipoprotein preferences of the E3 and E4 isoforms.
The Journal of biological chemistry 1994;269(35):22358-65.
1994: C Wilson; T Mau; Karl H Weisgraber; Mark Wardell; Robert W Mahley; David A Agard
Salt bridge relay triggers defective LDL receptor binding by a mutant apolipoprotein.
Structure (London, England : 1993) 1994;2(8):713-8.
1994: M B Braunfeld; Abraham Koster; John W Sedat; David A Agard
Cryo automated electron tomography: towards high-resolution reconstructions of plastic-embedded structures.
Journal of microscopy 1994;174(Pt 2):75-84.
1994: R K Dawe; John W Sedat; David A Agard; W Zacheus Cande
Meiotic chromosome pairing in maize is associated with a novel chromatin organization.
Cell 1994;76(5):901-12.
1993: David Baker; A K Shiau; David A Agard
The role of pro regions in protein folding.
Current opinion in cell biology 1993;5(6):966-70.
1993: David A Agard
To fold or not to fold....
Science (New York, N.Y.) 1993;260(5116):1903-4.
1993: J R Swedlow; David A Agard; John W Sedat
Chromosome structure inside the nucleus.
Current opinion in cell biology 1993;5(3):412-6.
1993: J R Swedlow; John W Sedat; David A Agard
Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopy.
Cell 1993;73(1):97-108.
1993: C Wilson; Lydia Gregoret; David A Agard
Modeling side-chain conformation for homologous proteins using an energy-based rotamer search.
Journal of molecular biology 1993;229(4):996-1006.
1993: Y Hiraoka; Abby F Dernburg; S J Parmelee; M C Rykowski; David A Agard; John W Sedat
The onset of homologous chromosome pairing during Drosophila melanogaster embryogenesis.
The Journal of cell biology 1993;120(3):591-600.
1992: Abraham Koster; H Chen; John W Sedat; David A Agard
Automated microscopy for electron tomography.
Ultramicroscopy 1992;46(1-4):207-27.
1992: Michael R Paddy; David A Agard; John W Sedat
An extended view of nuclear lamin structure, function, and dynamics.
Seminars in cell biology 1992;3(4):255-66.
1992: A Fujishige; K R Smith; J L Silen; David A Agard
Correct folding of alpha-lytic protease is required for its extracellular secretion from Escherichia coli.
The Journal of cell biology 1992;118(1):33-42.
1992: David Baker; J L Silen; David A Agard
Protease pro region required for folding is a potent inhibitor of the mature enzyme.
Proteins 1992;12(4):339-44.
1992: D Baker; Julie L Sohl; David A Agard
A protein-folding reaction under kinetic control.
Nature 1992;356(6366):263-5.
1991: R Bone; A Fujishige; C A Kettner; David A Agard
Structural basis for broad specificity in alpha-lytic protease mutants.
Biochemistry 1991;30(43):10388-98.
1991: C Wilson; J E Mace; David A Agard
Computational method for the design of enzymes with altered substrate specificity.
Journal of molecular biology 1991;220(2):495-506.
1991: C Wilson; Mark Wardell; Karl H Weisgraber; Robert W Mahley; David A Agard
Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E.
Science (New York, N.Y.) 1991;252(5014):1817-22.
1991: Z Kam; J S Minden; David A Agard; John W Sedat; Maria Leptin
Drosophila gastrulation: analysis of cell shape changes in living embryos by three-dimensional fluorescence microscopy.
Development (Cambridge, England) 1991;112(2):365-70.
1991: Y Hiraoka; J R Swedlow; Michael R Paddy; David A Agard; John W Sedat
Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena.
Seminars in cell biology 1991;2(3):153-65.
1991: R Bone; nicole Sampson; Paul A Bartlett; David A Agard
Crystal structures of alpha-lytic protease complexes with irreversibly bound phosphonate esters.
Biochemistry 1991;30(8):2263-72.
1991: J W Caldwell; David A Agard; Peter A Kollman
Free energy calculations on binding and catalysis by alpha-lytic protease: the role of substrate size in the P1 pocket.
Proteins 1991;10(2):140-8.
1990: Y Hiraoka; David A Agard; John W Sedat
Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos.
The Journal of cell biology 1990;111(6 Pt 2):2815-28.
1990: Michael R Paddy; Andrew S Belmont; H Saumweber; David A Agard; John W Sedat
Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery.
Cell 1990;62(1):89-106.
1989: Y Hiraoka; J S Minden; J R Swedlow; John W Sedat; David A Agard
Focal points for chromosome condensation and decondensation revealed by three-dimensional in vivo time-lapse microscopy.
Nature 1989;342(6247):293-6.
1989: J L Silen; David A Agard
The alpha-lytic protease pro-region does not require a physical linkage to activate the protease domain in vivo.
Nature 1989;341(6241):462-4.
1989: R Bone; D Frank; C A Kettner; David A Agard
Structural analysis of specificity: alpha-lytic protease complexes with analogues of reaction intermediates.
Biochemistry 1989;28(19):7600-9.
1989: J S Minden; David A Agard; John W Sedat; Bruce Alberts
Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos.
The Journal of cell biology 1989;109(2):505-16.
1989: Andrew S Belmont; M B Braunfeld; John W Sedat; David A Agard
Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro.
Chromosoma 1989;98(2):129-43.
1989: R Bone; J L Silen; David A Agard
Structural plasticity broadens the specificity of an engineered protease.
Nature 1989;339(6221):191-5.
1989: J L Silen; D Frank; A Fujishige; R Bone; David A Agard
Analysis of prepro-alpha-lytic protease expression in Escherichia coli reveals that the pro region is required for activity.
Journal of bacteriology 1989;171(3):1320-5.
1988: C A Kettner; R Bone; David A Agard; W W Bachovchin
Kinetic properties of the binding of alpha-lytic protease to peptide boronic acids.
Biochemistry 1988;27(20):7682-8.
1988: J L Silen; C N McGrath; K R Smith; David A Agard
Molecular analysis of the gene encoding alpha-lytic protease: evidence for a preproenzyme.
Gene 1988;69(2):237-44.
1988: M C Rykowski; S J Parmelee; David A Agard; John W Sedat
Precise determination of the molecular limits of a polytene chromosome band: regulatory sequences for the Notch gene are in the interband.
Cell 1988;54(4):461-72.
1987: R Bone; A B Shenvi; C A Kettner; David A Agard
Serine protease mechanism: structure of an inhibitory complex of alpha-lytic protease and a tightly bound peptide boronic acid.
Biochemistry 1987;26(24):7609-14.
1987: Y Hiraoka; John W Sedat; David A Agard
The use of a charge-coupled device for quantitative optical microscopy of biological structures.
Science (New York, N.Y.) 1987;238(4823):36-41.
1987: Andrew S Belmont; John W Sedat; David A Agard
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organization.
The Journal of cell biology 1987;105(1):77-92.