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Alberto Napuli

Research Profile

Concepts & Ideas

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X Ray Crystallography

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Amino Acid Sequences

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RNA Interference

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Publications

TOP 3
Eric T Larson; Frederick S Buckner; Erkang Fan; Wim G J Hol; Jessica E Kim; Alberto J Napuli; Wesley C Van Voorhis; Christophe L M J Verlinde; Frank H Zucker; Ethan A Merritt
Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia.
Garry W Buchko; Stephen N Hewitt; Alberto J Napuli; Wesley C Van Voorhis; Peter J Myler
Solution-state NMR structure and biophysical characterization of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis.
Garry W Buchko; Stephen N Hewitt; Alberto J Napuli; Wesley C Van Voorhis; Peter J Myler
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.

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All Publications

2012: Eric T Larson; Frederick S Buckner; Erkang Fan; Wim G J Hol; Jessica E Kim; Alberto J Napuli; Wesley C Van Voorhis; Christophe L M J Verlinde; Frank H Zucker; Ethan A Merritt
Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia.
Acta crystallographica. Section D, Biological crystallography 2012;68(Pt 9):1194-200.
2011: Garry W Buchko; Stephen N Hewitt; Alberto J Napuli; Wesley C Van Voorhis; Peter J Myler
Solution-state NMR structure and biophysical characterization of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1148-53.
2011: Garry W Buchko; Stephen N Hewitt; Alberto J Napuli; Wesley C Van Voorhis; Peter J Myler
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1129-36.
2011: Jan Abendroth; Janhavi Bhandari; Shellie Dieterich; Thomas E Edwards; Anna S Gardberg; Peter J Myler; Alberto J Napuli; Banumathi Sankaran; Bart L Staker; Wesley C Van Voorhis; Lance J Stewart
BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative β-lactamase-like protein' from Brucella melitensis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1106-12.
2011: Garry W Buchko; Thomas E Edwards; Stephen N Hewitt; Laura Law; Alberto J Napuli; Bart L Staker; Lance J Stewart; Wesley C Van Voorhis; Jan Abendroth; Tracy L Arakaki; Peter J Myler
Structure of a Nudix hydrolase (MutT) in the Mg(2+)-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1078-83.
2011: Cassie M Bryan; Ryan Choi; Thomas E Edwards; Angela Kelley; David J Leibly; Alberto J Napuli; Janhavi Bhandari; Wesley C Van Voorhis; Lance J Stewart
High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1010-4.
2011: Stephen N Hewitt; Angela Kelley; Alberto J Napuli; Ryan Choi; Gregory J Crowther; Wesley C Van Voorhis
Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 9):1006-9.
2011: Eric T Larson; Jessica E Kim; Lisa J Castaneda; Alberto J Napuli; Zhongsheng Zhang; Erkang Fan; Frank H Zucker; Christophe L M J Verlinde; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol; Ethan A Merritt
The double-length tyrosyl-tRNA synthetase from the eukaryote Leishmania major forms an intrinsically asymmetric pseudo-dimer.
Journal of molecular biology 2011;409(2):159-76.
2011: Sayaka Shibata; John R Gillespie; Angela M Kelley; Alberto J Napuli; Zhongsheng Zhang; Kuzma V Kovzun; Ranae M Pefley; Jocelyn Lam; Frank H Zucker; Wesley C Van Voorhis; Ethan A Merritt; Wim G J Hol; Christophe L M J Verlinde; Erkang Fan; Frederick S Buckner
Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice.
Antimicrobial agents and chemotherapy 2011;55(5):1982-9.
2011: Ethan A Merritt; Tracy L Arakaki; Robert Gillespie; Alberto J Napuli; Jessica E Kim; Frederick S Buckner; Wesley C Van Voorhis; Christophe L M J Verlinde; Erkang Fan; Frank Zucker; Wim G J Hol
Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase.
Molecular and biochemical parasitology 2011;177(1):20-8.
2011: Kayode K Ojo; Tracy L Arakaki; Alberto J Napuli; Krishna K Inampudi; Katelyn R Keyloun; Li Zhang; Wim G J Hol; Christophe L M J Verlinde; Ethan A Merritt; Wesley C Van Voorhis
Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3.
Molecular and biochemical parasitology 2011;176(2):98-108.
2011: Eric T Larson; Jessica E Kim; Frank H Zucker; Angela Kelley; Natascha Mueller; Alberto J Napuli; Christophe L M J Verlinde; Erkang Fan; Frederick S Buckner; Wesley C Van Voorhis; Ethan A Merritt; Wim G J Hol
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate.
Biochimie 2011;93(3):570-82.
2011: Gregory J Crowther; Alberto J Napuli; James H Gilligan; Kerstin Gagaring; Rachel Borboa; Carolyn Francek; Zhong Chen; Eleanor F Dagostino; Justin B Stockmyer; Yu Wang; Philip P Rodenbough; Lisa J Castaneda; David J Leibly; Janhavi Bhandari; Michael H Gelb; Achim Brinker; Ingo H Engels; Jennifer Taylor; Arnab K Chatterjee; Pascal Fantauzzi; Richard J Glynne; Wesley C Van Voorhis; Kelli L Kuhen
Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds.
Molecular and biochemical parasitology 2011;175(1):21-9.
2010: Tracy L Arakaki; Megan Carter; Alberto J Napuli; Christophe L M J Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol; Ethan A Merritt
The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs.
Journal of structural biology 2010;171(2):238-43.
2010: Frank H Zucker; Jaclyn dela Rosa; Tracy L Arakaki; Frederick S Buckner; Lisa J Castaneda; Erkang Fan; Jessica Kim; Eric T Larson; Ethan A Merritt; Natascha Mueller; Stephen R Nakazawa Hewitt; Alberto J Napuli; Jenni Ross; Christine Stewart; Easwara Subramanian; Wesley C Van Voorhis; Christophe L M J Verlinde; Liren Xiao; Li Zhang; Wim G J Hol
Prediction of protein crystallization outcome using a hybrid method.
Journal of structural biology 2010;171(1):64-73.
2010: Kayode K Ojo; Eric T Larson; Katelyn R Keyloun; Lisa J Castaneda; Amy E Derocher; Krishna K Inampudi; Jessica E Kim; Tracy L Arakaki; Ryan C Murphy; Li Zhang; Alberto J Napuli; Dustin J Maly; Christophe Verlinde; Frederick S Buckner; Marilyn Parsons; Wim G J Hol; Ethan A Merritt; Wesley C Van Voorhis
Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors.
Nature structural & molecular biology 2010;17(5):602-7.
2010: Gregory J Crowther; Panqing He; Philip P Rodenbough; Andrew P Thomas; Kuzma V Kovzun; David J Leibly; Janhavi Bhandari; Lisa J Castaneda; Wim G J Hol; Michael H Gelb; Alberto J Napuli; Wesley C Van Voorhis
Use of thermal melt curves to assess the quality of enzyme preparations.
Analytical biochemistry 2010;399(2):268-75.
2010: Ethan A Merritt; Tracy L Arakaki; John R Gillespie; Eric T Larson; Angela Kelley; Natascha Mueller; Alberto J Napuli; Jessica Kim; Li Zhang; Christophe Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs.
Journal of molecular biology 2010;397(2):481-94.
2010: Eric T Larson; Devaraja G Mudeppa; John R Gillespie; Natascha Mueller; Alberto J Napuli; Jennifer A Arif; Jenni Ross; Tracy L Arakaki; Angela Lauricella; George deTitta; Joseph Luft; Frank Zucker; Christophe Verlinde; Erkang Fan; Wesley C Van Voorhis; Frederick S Buckner; Pradipsinh K Rathod; Wim G J Hol; Ethan A Merritt
The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase.
Journal of molecular biology 2010;396(5):1244-59.
2010: Jan Abendroth; Michael S McCormick; Thomas E Edwards; Bart Staker; Roderick Loewen; Martin Gifford; Jeff Rifkin; Chad Mayer; Wenjin Guo; Yang Zhang; Peter Myler; Angela Kelley; Erwin Analau; Stephen Nakazawa Hewitt; Alberto J Napuli; Peter Kuhn; Ronald D Ruth; Lance J Stewart
X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
Journal of structural and functional genomics 2010;11(1):91-100.
2010: Ethan A Merritt; Tracy L Arakaki; Eric T Larson; Angela Kelley; Natascha Mueller; Alberto J Napuli; Li Zhang; George Deditta; Joseph Luft; Christophe Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol
Crystal structure of the aspartyl-tRNA synthetase from Entamoeba histolytica.
Molecular and biochemical parasitology 2010;169(2):95-100.
2009: Garry W Buchko; Stephen N Hewitt; Alberto J Napuli; Wesley C Van Voorhis; Peter J Myler
Backbone and side chain (1)H, (13)C, and (15)N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei.
Biomolecular NMR assignments 2009;3(2):163-6.
2009: Gregory J Crowther; Alberto J Napuli; Andrew P Thomas; Diana J Chung; Kuzma V Kovzun; David J Leibly; Lisa J Castaneda; Janhavi Bhandari; Christopher J Damman; Raymond Hui; Wim G J Hol; Frederick S Buckner; Christophe Verlinde; Zhongsheng Zhang; Erkang Fan; Wesley C Van Voorhis
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands.
Journal of biomolecular screening 2009;14(6):700-7.
2008: Cory J Gerdts; Mark Elliott; Scott Lovell; Mark B Mixon; Alberto J Napuli; Bart L Staker; peter nollert; Lance Stewart
The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).
Acta crystallographica. Section D, Biological crystallography 2008;64(Pt 11):1116-22.
2008: Kayode K Ojo; John R Gillespie; Aaron J Riechers; Alberto J Napuli; Christophe Verlinde; Frederick S Buckner; Michael H Gelb; Mathias M Domostoj; Susan J Wells; Alexander Scheer; Timothy Wells; Wesley C Van Voorhis
Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy.
Antimicrobial agents and chemotherapy 2008;52(10):3710-7.
2003: Chih-Wen Peng; Alberto J Napuli; Valerian V Dolja
Leader proteinase of beet yellows virus functions in long-distance transport.
Journal of virology 2003;77(5):2843-9.
2003: Alberto J Napuli; Dina V Alzhanova; Catalin E Doneanu; Douglas F Barofsky; Eugene V Koonin; Valerian V Dolja
The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails.
Journal of virology 2003;77(4):2377-84.
2002: Alexey I Prokhnevsky; Valera V Peremyslov; Alberto J Napuli; Valerian V Dolja
Interaction between long-distance transport factor and Hsp70-related movement protein of Beet yellows virus.
Journal of virology 2002;76(21):11003-11.
2001: Dina V Alzhanova; Alberto J Napuli; R Creamer; Valerian V Dolja
Cell-to-cell movement and assembly of a plant closterovirus: roles for the capsid proteins and Hsp70 homolog.
The EMBO journal 2001;20(24):6997-7007.
2000: Alberto J Napuli; B W Falk; Valerian V Dolja
Interaction between HSP70 homolog and filamentous virions of the Beet yellows virus.
Virology 2000;274(1):232-9.