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Bernd Nidetzky

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Patricia Bubner; Harald Plank; Bernd Nidetzky
Visualizing cellulase activity.
Birgit Wilding; Margit Winkler; Sigrid Egger; Karl Gruber; Regina Kratzer; Andrzej Lyskowski; Bernd Nidetzky; Barbara Petschacher; Georg Steinkellner; Norbert Klempier
Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering.
Juan M Bolivar; Tanja Consolati; Torsten Mayr; Bernd Nidetzky
Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts.

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2013: Patricia Bubner; Harald Plank; Bernd Nidetzky
Visualizing cellulase activity.
Biotechnology and bioengineering 2013;110(6):1529-49.
2013: Birgit Wilding; Margit Winkler; Sigrid Egger; Karl Gruber; Regina Kratzer; Andrzej Lyskowski; Bernd Nidetzky; Barbara Petschacher; Georg Steinkellner; Norbert Klempier
Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering.
Chemistry (Weinheim an der Bergstrasse, Germany) 2013;19(22):7007-12.
2013: Juan M Bolivar; Tanja Consolati; Torsten Mayr; Bernd Nidetzky
Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts.
Trends in biotechnology 2013;31(3):194-203.
2013: Vera Novy; Mario Klimacek; Stefan Krahulec; Karin Longus; Bernd Nidetzky
Co-fermentation of hexose and pentose sugars in a spent sulfite liquor matrix with genetically modified Saccharomyces cerevisiae.
Bioresource technology 2013;130():439-48.
2013: Michael Brunsteiner; Michaela Flock; Bernd Nidetzky
Structure based descriptors for the estimation of colloidal interactions and protein aggregation propensities.
PloS one 2013;8(4):e59797.
2013: Mareike Monschein; Christoph Reisinger; Bernd Nidetzky
Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: a detailed comparison using convenient kinetic analysis.
Bioresource technology 2013;128():679-87.
2012: Thomas Ganner; Claudia Mayrhofer; Patricia Bubner; Manuel Eibinger; Harald Plank; Bernd Nidetzky
Dissecting and reconstructing synergism: in situ visualization of cooperativity among cellulases.
The Journal of biological chemistry 2012;287(52):43215-22.
2012: Alexander Gutmann; Bernd Nidetzky
Switching between O- and C-glycosyltransferase through exchange of active-site motifs.
Angewandte Chemie (International ed. in English) 2012;51(51):12879-83.
2012: Michael Hanscho; Harald F Hofbauer; Sepp D Kohlwein; Stefan Krahulec; Bernd Nidetzky; David E Ruckerbauer; Juergen Zanghellini; Neha Chauhan; klaus natter
Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth.
FEMS yeast research 2012;12(7):796-808.
2012: Michael Vogl; Regina Kratzer; Bernd Nidetzky; Lothar Brecker
Candida tenuis xylose reductase catalyzed reduction of aryl ketones for enantioselective synthesis of active oxetine derivatives.
Chirality 2012;24(10):847-53.
2012: Thomas Eixelsberger; Kathryn L Kavanagh; Lothar Brecker; Michael Brunsteiner; Sigrid Egger; Udo Oppermann; Sabine Sykora; Bernd Nidetzky
Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid.
The Journal of biological chemistry 2012;287(37):31349-58.
2012: Ulrich Roessl; Johanna Wiesbauer; Ruth Birner-Gruenberger; Stefan Leitgeb; Bernd Nidetzky
Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions.
Biotechnology journal 2012;7(8):1014-24.
2012: Birgit Unterweger; Thomas Stoisser; Ruth Birner-Gruenberger; Stefan Leitgeb; Bernd Nidetzky
Engineering of Aerococcus viridans L-lactate oxidase for site-specific PEGylation: characterization and selective bioorthogonal modification of a S218C mutant.
Bioconjugate chemistry 2012;23(7):1406-14.
2012: Patricia Wildberger; Lothar Brecker; Bernd Nidetzky
Examining the role of phosphate in glycosyl transfer reactions of Cellulomonas uda cellobiose phosphorylase using D-glucal as donor substrate.
Carbohydrate research 2012;356():224-32.
2012: Thomas Eixelsberger; Lothar Brecker; Bernd Nidetzky
Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction.
Carbohydrate research 2012;356():209-14.
2012: Juan M Bolivar; Bernd Nidetzky
Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports.
Langmuir : the ACS journal of surfaces and colloids 2012;28(26):10040-9.
2012: Datta Madamwar; Bernd Nidetzky
Preface--biocatalysis.
Bioresource technology 2012;115():1.
2012: Juan M Bolivar; Bernd Nidetzky
Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Z basic2: design of a heterogeneous D-amino acid oxidase catalyst on porous glass.
Biotechnology and bioengineering 2012;109(6):1490-8.
2012: Stefan Krahulec; Mario Klimacek; Bernd Nidetzky
Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae.
Journal of biotechnology 2012;158(4):192-202.
2012: Stefan Krahulec; Regina Kratzer; Karin Longus; Bernd Nidetzky
Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation.
MicrobiologyOpen 2012;1(1):64-70.
2012: Katharina Schmölzer; Katharina Mädje; Bernd Nidetzky; Regina Kratzer
Bioprocess design guided by in situ substrate supply and product removal: process intensification for synthesis of (S)-1-(2-chlorophenyl)ethanol.
Bioresource technology 2012;108():216-23.
2012: Mario Klimacek; Michael Brunsteiner; Bernd Nidetzky
Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens: mobile GLU292 controls proton relay through a water channel that connects the active site with bulk solvent.
The Journal of biological chemistry 2012;287(9):6655-67.
2012: Patricia Bubner; Judith Dohr; Claudia Mayrhofer; Harald Plank; Bernd Nidetzky
Cellulases dig deep: in situ observation of the mesoscopic structural dynamics of enzymatic cellulose degradation.
The Journal of biological chemistry 2012;287(4):2759-65.
2012: Sigrid Egger; Kathryn L Kavanagh; Mario Klimacek; Apirat Chaikuad; Udo Oppermann; Bernd Nidetzky
Structural and kinetic evidence that catalytic reaction of human UDP-glucose 6-dehydrogenase involves covalent thiohemiacetal and thioester enzyme intermediates.
The Journal of biological chemistry 2012;287(3):2119-29.
2012: Caterina Boniello; Torsten Mayr; Juan M Bolivar; Bernd Nidetzky
Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts.
BMC biotechnology 2012;12():11.
2012: Katharina Mädje; Bernd Nidetzky; Katharina Schmölzer; Regina Kratzer
Host cell and expression engineering for development of an E. coli ketoreductase catalyst: enhancement of formate dehydrogenase activity for regeneration of NADH.
Microbial cell factories 2012;11():7.
2011: Simone L Pival; Ruth Birner-Gruenberger; Corinna Krump; Bernd Nidetzky
D-Xylulose kinase from Saccharomyces cerevisiae: isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli.
Protein expression and purification 2011;79(2):223-30.
2011: Michael Vogl; Regina Kratzer; Bernd Nidetzky; Lothar Brecker
Candida tenuis xylose reductase catalysed reduction of acetophenones: the effect of ring-substituents on catalytic efficiency.
Organic & biomolecular chemistry 2011;9(16):5863-70.
2011: Sigrid Egger; Kathryn L Kavanagh; Apirat Chaikuad; Udo Oppermann; Bernd Nidetzky
Structure and mechanism of human UDP-glucose 6-dehydrogenase.
The Journal of biological chemistry 2011;286(27):23877-87.
2011: Juan M Bolivar; Johanna Wiesbauer; Bernd Nidetzky
Biotransformations in microstructured reactors: more than flowing with the stream?
Trends in biotechnology 2011;29(7):333-42.
2011: Christiane Luley-Goedl; Bernd Nidetzky
Glycosides as compatible solutes: biosynthesis and applications.
Natural product reports 2011;28(5):875-96.
2011: Regina Kratzer; Matej Pukl; Sigrid Egger; Michael Vogl; Lothar Brecker; Bernd Nidetzky
Enzyme identification and development of a whole-cell biotransformation for asymmetric reduction of o-chloroacetophenone.
Biotechnology and bioengineering 2011;108(4):797-803.
2011: Stefan Krahulec; Guilliano Cem Armao; Mario Klimacek; Bernd Nidetzky
Enzymes of mannitol metabolism in the human pathogenic fungus Aspergillus fumigatus--kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications.
The FEBS journal 2011;278(8):1264-76.
2011: Sandra Abad; Jozef Nahalka; Margit Winkler; Gabriele Bergler; Robert Speight; Anton Glieder; Bernd Nidetzky
High-level expression of Rhodotorula gracilis D-amino acid oxidase in Pichia pastoris.
Biotechnology letters 2011;33(3):557-63.
2011: Patricia Wildberger; Christiane Luley-Goedl; Bernd Nidetzky
Aromatic interactions at the catalytic subsite of sucrose phosphorylase: their roles in enzymatic glucosyl transfer probed with Phe52→Ala and Phe52→Asn mutants.
FEBS letters 2011;585(3):499-504.
2010: Christiane Luley-Goedl; Bernd Nidetzky
Carbohydrate synthesis by disaccharide phosphorylases: reactions, catalytic mechanisms and application in the glycosciences.
Biotechnology journal 2010;5(12):1324-38.
2010: Mario Klimacek; Stefan Krahulec; Uwe Sauer; Bernd Nidetzky
Limitations in xylose-fermenting Saccharomyces cerevisiae, made evident through comprehensive metabolite profiling and thermodynamic analysis.
Applied and environmental microbiology 2010;76(22):7566-74.
2010: Sigrid Egger; Apirat Chaikuad; Kathryn L Kavanagh; Udo Oppermann; Bernd Nidetzky
UDP-glucose dehydrogenase: structure and function of a potential drug target.
Biochemical Society transactions 2010;38(5):1378-85.
2010: Mario Klimacek; Bernd Nidetzky
From alcohol dehydrogenase to a "one-way" carbonyl reductase by active-site redesign: a mechanistic study of mannitol 2-dehydrogenase from pseudomonas fluorescens.
The Journal of biological chemistry 2010;285(40):30644-53.
2010: Christiane Luley-Goedl; Thornthan Sawangwan; Lothar Brecker; Patricia Wildberger; Bernd Nidetzky
Regioselective O-glucosylation by sucrose phosphorylase: a promising route for functional diversification of a range of 1,2-propanediols.
Carbohydrate research 2010;345(12):1736-40.
2010: Christiane Luley-Goedl; Bernd Nidetzky
Small-molecule glucosylation by sucrose phosphorylase: structure-activity relationships for acceptor substrates revisited.
Carbohydrate research 2010;345(10):1492-6.
2010: Mario Mueller; Regina Kratzer; Margaretha Schiller; Anita Slavica; Gerald Rechberger; Manfred Kollroser; Bernd Nidetzky
The role of Cys108 in Trigonopsis variabilis d-amino acid oxidase examined through chemical oxidation studies and point mutations C108S and C108D.
Biochimica et biophysica acta 2010;1804(7):1483-91.
2010: Caterina Boniello; Torsten Mayr; Ingo Klimant; Burghard Koenig; Waander Riethorst; Bernd Nidetzky
Intraparticle concentration gradients for substrate and acidic product in immobilized cephalosporin C amidase and their dependencies on carrier characteristics and reaction parameters.
Biotechnology and bioengineering 2010;106(4):528-40.
2010: Stefan Leitgeb; Bernd Nidetzky
Enzyme catalytic promiscuity: the nonheme Fe2+ center of beta-diketone-cleaving dioxygenase Dke1 promotes hydrolysis of activated esters.
Chembiochem : a European journal of chemical biology 2010;11(4):502-5.
2010: Ulrich Roessl; Jozef Nahálka; Bernd Nidetzky
Carrier-free immobilized enzymes for biocatalysis.
Biotechnology letters 2010;32(3):341-50.
2010: Grit D Straganz; Adrienne R Diebold; Sigrid Egger; Bernd Nidetzky; Edward I Solomon
Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis.
Biochemistry 2010;49(5):996-1004.
2010: Thornthan Sawangwan; Christiane Goedl; Bernd Nidetzky
Glucosylglycerol and glucosylglycerate as enzyme stabilizers.
Biotechnology journal 2010;5(2):187-91.
2010: Mario Klimacek; Bernd Nidetzky
The oxyanion hole of Pseudomonas fluorescens mannitol 2-dehydrogenase: a novel structural motif for electrostatic stabilization in alcohol dehydrogenase active sites.
The Biochemical journal 2010;425(2):455-63.
2010: Sandra Abad; Jozef Nahalka; Gabriele Bergler; S Alison Arnold; Robert Speight; Ian Fotheringham; Bernd Nidetzky; Anton Glieder
Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst.
Microbial cell factories 2010;9():24.
2010: Stefan Krahulec; Barbara Petschacher; Michael Wallner; Karin Longus; Mario Klimacek; Bernd Nidetzky
Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization.
Microbial cell factories 2010;9():16.
2010: Mario Mueller; Bernd Nidetzky
Orthophosphate binding at the dimer interface of Corynebacterium callunae starch phosphorylase: mutational analysis of its role for activity and stability of the enzyme.
BMC biochemistry 2010;11():8.
2009: Mario Mueller; Ryo Takemasa; Alexandra Schwarz; Haruyuki Atomi; Bernd Nidetzky
"Short-chain" alpha-1,4-glucan phosphorylase having a truncated N-terminal domain: functional expression and characterization of the enzyme from Sulfolobus solfataricus.
Biochimica et biophysica acta 2009;1794(11):1709-14.
2009: Thornthan Sawangwan; Christiane Goedl; Bernd Nidetzky
Single-step enzymatic synthesis of (R)-2-O-alpha-D-glucopyranosyl glycerate, a compatible solute from micro-organisms that functions as a protein stabiliser.
Organic & biomolecular chemistry 2009;7(20):4267-70.
2009: Stefan Leitgeb; Grit D Straganz; Bernd Nidetzky
Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves beta-diketones.
The FEBS journal 2009;276(20):5983-97.
2009: Christiane Goedl; Bernd Nidetzky
Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate.
Chembiochem : a European journal of chemical biology 2009;10(14):2333-7.
2009: Alexandra Schwarz; Malene S Thomsen; Bernd Nidetzky
Enzymatic synthesis of beta-glucosylglycerol using a continuous-flow microreactor containing thermostable beta-glycoside hydrolase CelB immobilized on coated microchannel walls.
Biotechnology and bioengineering 2009;103(5):865-72.
2009: Simone L Pival; Mario Klimacek; Bernd Nidetzky
The catalytic mechanism of NADH-dependent reduction of 9,10-phenanthrenequinone by Candida tenuis xylose reductase reveals plasticity in an aldo-keto reductase active site.
The Biochemical journal 2009;421(1):43-9.
2009: Stefan Krahulec; Mario Klimacek; Bernd Nidetzky
Engineering of a matched pair of xylose reductase and xylitol dehydrogenase for xylose fermentation by Saccharomyces cerevisiae.
Biotechnology journal 2009;4(5):684-94.
2009: Lothar Brecker; Marek Mahut; Alexandra Schwarz; Bernd Nidetzky
In situ proton NMR study of acetyl and formyl group migration in mono-O-acyl D-glucose.
Magnetic resonance in chemistry : MRC 2009;47(4):328-32.
2009: Stefan Krahulec; Guilliano C Armao; Patricia Bubner; Mario Klimacek; Bernd Nidetzky
Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase.
Chemico-biological interactions 2009;178(1-3):274-82.
2009: Stefan Leitgeb; Grit D Straganz; Bernd Nidetzky
Biochemical characterization and mutational analysis of the mononuclear non-haem Fe2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii.
The Biochemical journal 2009;418(2):403-11.
2009: Malene S Thomsen; Bernd Nidetzky
Coated-wall microreactor for continuous biocatalytic transformations using immobilized enzymes.
Biotechnology journal 2009;4(1):98-107.
2008: Simone L Pival; Mario Klimacek; Regina Kratzer; Bernd Nidetzky
Tyr-51 is the proton donor-acceptor for NAD(H)-dependent interconversion of xylose and xylitol by Candida tenuis xylose reductase (AKR2B5).
FEBS letters 2008;582(29):4095-9.
2008: Stefan Leitgeb; Bernd Nidetzky
Structural and functional comparison of 2-His-1-carboxylate and 3-His metallocentres in non-haem iron(II)-dependent enzymes.
Biochemical Society transactions 2008;36(Pt 6):1180-6.
2008: Regina Kratzer; Sigrid Egger; Bernd Nidetzky
Integration of enzyme, strain and reaction engineering to overcome limitations of baker's yeast in the asymmetric reduction of alpha-keto esters.
Biotechnology and bioengineering 2008;101(5):1094-101.
2008: Christiane Goedl; Alexandra Schwarz; Mario Mueller; Lothar Brecker; Bernd Nidetzky
Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase.
Carbohydrate research 2008;343(12):2032-40.
2008: Lothar Brecker; Alexandra Schwarz; Christiane Goedl; Regina Kratzer; Catrin E Tyl; Bernd Nidetzky
Studying non-covalent enzyme carbohydrate interactions by STD NMR.
Carbohydrate research 2008;343(12):2153-61.
2008: Stefan Krahulec; Guilliano C Armao; Hansjörg Weber; Mario Klimacek; Bernd Nidetzky
Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate.
Carbohydrate research 2008;343(9):1414-23.
2008: Christiane Goedl; Bernd Nidetzky
The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site-directed mutagenesis and chemical rescue studies.
The FEBS journal 2008;275(5):903-13.
2008: Jozef Nahalka; Iskandar Dib; Bernd Nidetzky
Encapsulation of Trigonopsis variabilis D-amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme.
Biotechnology and bioengineering 2008;99(2):251-60.
2008: Patricia Bubner; Mario Klimacek; Bernd Nidetzky
Structure-guided engineering of the coenzyme specificity of Pseudomonas fluorescens mannitol 2-dehydrogenase to enable efficient utilization of NAD(H) and NADP(H).
FEBS letters 2008;582(2):233-7.
2008: Regina Kratzer; Matej Pukl; Sigrid Egger; Bernd Nidetzky
Whole-cell bioreduction of aromatic alpha-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli.
Microbial cell factories 2008;7():37.
2008: Iskandar Dib; Bernd Nidetzky
The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis.
BMC biotechnology 2008;8():72.
2008: Barbara Petschacher; Bernd Nidetzky
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae.
Microbial cell factories 2008;7():9.
2007: Alexandra Schwarz; Lothar Brecker; Bernd Nidetzky
Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334-->Gly mutant enzymes.
The FEBS journal 2007;274(19):5105-15.
2007: Mario Mueller; Bernd Nidetzky
Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase.
FEBS letters 2007;581(20):3814-8.
2007: Malene S Thomsen; Peter Pölt; Bernd Nidetzky
Development of a microfluidic immobilised enzyme reactor.
Chemical communications (Cambridge, England) 2007;(24):2527-9.
2007: Mario Klimacek; Heidemarie Hellmer; Bernd Nidetzky
Catalytic mechanism of Zn2+-dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu154-->Cys forms of yeast xylitol dehydrogenase.
The Biochemical journal 2007;404(3):421-9.
2007: Jozef Nahalka; Bernd Nidetzky
Fusion to a pull-down domain: a novel approach of producing Trigonopsis variabilisD-amino acid oxidase as insoluble enzyme aggregates.
Biotechnology and bioengineering 2007;97(3):454-61.
2007: Alexandra Schwarz; Lothar Brecker; Bernd Nidetzky
Acid-base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237-->Gln mutant enzymes.
The Biochemical journal 2007;403(3):441-9.
2007: Mario Mueller; Bernd Nidetzky
The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis.
FEBS letters 2007;581(7):1403-8.
2007: Bernd Nidetzky; Helmut Schwab
Special issue: enzyme technology and biocatalysis.
Journal of biotechnology 2007;129(1):1-2.
2007: Alexandra Schwarz; Christiane Goedl; Alphonse Minani; Bernd Nidetzky
Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose.
Journal of biotechnology 2007;129(1):140-50.
2007: Christiane Goedl; Alexandra Schwarz; Alphonse Minani; Bernd Nidetzky
Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate.
Journal of biotechnology 2007;129(1):77-86.
2007: Thomas Kamrat; Bernd Nidetzky
Entrapment in E. coli improves the operational stability of recombinant beta-glycosidase CelB from Pyrococcus furiosus and facilitates biocatalyst recovery.
Journal of biotechnology 2007;129(1):69-76.
2007: Regina Kratzer; Bernd Nidetzky
Identification of Candida tenuis xylose reductase as highly selective biocatalyst for the synthesis of aromatic alpha-hydroxy esters and improvement of its efficiency by protein engineering.
Chemical communications (Cambridge, England) 2007;(10):1047-9.
2007: eric di Luccio; Barbara Petschacher; Jennifer Voegtli; Chou Hui-Ting; Henning Stahlberg; Bernd Nidetzky; David K Wilson
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli.
Journal of molecular biology 2007;365(3):783-98.
2007: Iskandar Dib; Damir Stanzer; Bernd Nidetzky
Trigonopsis variabilis D-amino acid oxidase: control of protein quality and opportunities for biocatalysis through production in Escherichia coli.
Applied and environmental microbiology 2007;73(1):331-3.
2007: Anita Slavica; Iskandar Dib; Bernd Nidetzky
Selective modification of surface-exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme.
Biotechnology and bioengineering 2007;96(1):9-17.
2006: Markus Lang; Thomas Kamrat; Bernd Nidetzky
Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable beta-glycosylhydrolase CelB from Pyrococcus Furiosus.
Biotechnology and bioengineering 2006;95(6):1093-100.
2006: Grit D Straganz; Bernd Nidetzky
Variations of the 2-His-1-carboxylate theme in mononuclear non-heme FeII oxygenases.
Chembiochem : a European journal of chemical biology 2006;7(10):1536-48.
2006: Regina Kratzer; David K Wilson; Bernd Nidetzky
Catalytic mechanism and substrate selectivity of aldo-keto reductases: insights from structure-function studies of Candida tenuis xylose reductase.
IUBMB life 2006;58(9):499-507.
2006: Christiane Goedl; Richard Griessler; Alexandra Schwarz; Bernd Nidetzky
Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases.
The Biochemical journal 2006;397(3):491-500.
2006: Alexandra Schwarz; Bernd Nidetzky
Asp-196-->Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate.
FEBS letters 2006;580(16):3905-10.
2006: Iskandar Dib; Anita Slavica; Waander Riethorst; Bernd Nidetzky
Thermal inactivation of D-amino acid oxidase from Trigonopsis variabilis occurs via three parallel paths of irreversible denaturation.
Biotechnology and bioengineering 2006;94(4):645-54.
2006: Regina Kratzer; Stefan Leitgeb; David K Wilson; Bernd Nidetzky
Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis.
The Biochemical journal 2006;393(Pt 1):51-8.
2005: Anita Slavica; Iskandar Dib; Bernd Nidetzky
Single-site oxidation, cysteine 108 to cysteine sulfinic acid, in D-amino acid oxidase from Trigonopsis variabilis and its structural and functional consequences.
Applied and environmental microbiology 2005;71(12):8061-8.
2005: Barbara Petschacher; Bernd Nidetzky
Engineering Candida tenuis Xylose reductase for improved utilization of NADH: antagonistic effects of multiple side chain replacements and performance of site-directed mutants under simulated in vivo conditions.
Applied and environmental microbiology 2005;71(10):6390-3.
2005: Grit D Straganz; Bernd Nidetzky
Reaction coordinate analysis for beta-diketone cleavage by the non-heme Fe2+-dependent dioxygenase Dke1.
Journal of the American Chemical Society 2005;127(35):12306-14.
2005: Regina Kratzer; Bernd Nidetzky
Electrostatic stabilization in a pre-organized polar active site: the catalytic role of Lys-80 in Candida tenuis xylose reductase (AKR2B5) probed by site-directed mutagenesis and functional complementation studies.
The Biochemical journal 2005;389(Pt 2):507-15.
2005: Alexandra Schwarz; Francesco-Maria Pierfederici; Bernd Nidetzky
Catalytic mechanism of alpha-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed mutants.
The Biochemical journal 2005;387(Pt 2):437-45.
2005: Stefan Leitgeb; Barbara Petschacher; David K Wilson; Bernd Nidetzky
Fine tuning of coenzyme specificity in family 2 aldo-keto reductases revealed by crystal structures of the Lys-274-->Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+.
FEBS letters 2005;579(3):763-7.
2005: Barbara Petschacher; Stefan Leitgeb; Kathryn L Kavanagh; David K Wilson; Bernd Nidetzky
The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography.
The Biochemical journal 2005;385(Pt 1):75-83.
2004: Grit D Straganz; Hannes Hofer; Walter Steiner; Bernd Nidetzky
Electronic substituent effects on the cleavage specificity of a non-heme Fe(2+)-dependent beta-diketone dioxygenase and their mechanistic implications.
Journal of the American Chemical Society 2004;126(39):12202-3.
2004: Richard Griessler; Barbara Psik; Alexandra Schwarz; Bernd Nidetzky
Relationships between structure, function and stability for pyridoxal 5'-phosphate-dependent starch phosphorylase from Corynebacterium callunae as revealed by reversible cofactor dissociation studies.
European journal of biochemistry / FEBS 2004;271(16):3319-29.
2004: Regina Kratzer; Kathryn L Kavanagh; David K Wilson; Bernd Nidetzky
Studies of the enzymic mechanism of Candida tenuis xylose reductase (AKR 2B5): X-ray structure and catalytic reaction profile for the H113A mutant.
Biochemistry 2004;43(17):4944-54.
2003: Bernd Nidetzky; Kaspar Brüggler; Regina Kratzer; Peter Mayr
Multiple forms of xylose reductase in Candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies.
Journal of agricultural and food chemistry 2003;51(27):7930-5.
2003: Bernd Nidetzky; Richard Griessler; Francesco-Maria Pierfederici; Barbara Psik; Andrea Sciré; Fabio Tanfani
Mutagenesis of the dimer interface region of Corynebacterium callunae starch phosphorylase perturbs the phosphate-dependent conformational relay that enhances oligomeric stability of the enzyme.
Journal of biochemistry 2003;134(4):599-606.
2003: Mario Klimacek; Kathryn L Kavanagh; David K Wilson; Bernd Nidetzky
On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase.
The Biochemical journal 2003;375(Pt 1):141-9.
2003: Kathryn L Kavanagh; Mario Klimacek; Bernd Nidetzky; David K Wilson
Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases.
The Biochemical journal 2003;373(Pt 2):319-26.
2003: Richard Griessler; Alexandra Schwarz; Jan Mucha; Bernd Nidetzky
Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae. Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis.
European journal of biochemistry / FEBS 2003;270(10):2126-36.
2003: Peter Mayr; Barbara Petschacher; Bernd Nidetzky
Xylose reductase from the Basidiomycete fungus Cryptococcus flavus: purification, steady-state kinetic characterization, and detailed analysis of the substrate binding pocket using structure-activity relationships.
Journal of biochemistry 2003;133(4):553-62.
2003: Mario Klimacek; Kathryn L Kavanagh; David K Wilson; Bernd Nidetzky
Pseudomonas fluorescens mannitol 2-dehydrogenase and the family of polyol-specific long-chain dehydrogenases/reductases: sequence-based classification and analysis of structure-function relationships.
Chemico-biological interactions 2003;143-144():559-82.
2003: Kathryn L Kavanagh; Mario Klimacek; Bernd Nidetzky; David K Wilson
Crystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase: evidence for a very divergent long-chain dehydrogenase family.
Chemico-biological interactions 2003;143-144():551-8.
2003: Bernd Nidetzky; Heidemarie Helmer; Mario Klimacek; Regina Lunzer; Gerhard Mayer
Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli.
Chemico-biological interactions 2003;143-144():533-42.
2003: Mario Klimacek; Franz Wührer; Kathryn L Kavanagh; David K Wilson; Bernd Nidetzky
Altering dimer contacts in xylose reductase from Candida tenuis by site-directed mutagenesis: structural and functional properties of R180A mutant.
Chemico-biological interactions 2003;143-144():523-32.
2003: David K Wilson; Kathryn L Kavanagh; Mario Klimacek; Bernd Nidetzky
The xylose reductase (AKR2B5) structure: homology and divergence from other aldo-keto reductases and opportunities for protein engineering.
Chemico-biological interactions 2003;143-144():515-21.
2002: Kathryn L Kavanagh; Mario Klimacek; Bernd Nidetzky; David K Wilson
Crystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase binary and ternary complexes. Specificity and catalytic mechanism.
The Journal of biological chemistry 2002;277(45):43433-42.
2002: Mario Klimacek; Bernd Nidetzky
A catalytic consensus motif for D-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens.
The Biochemical journal 2002;367(Pt 1):13-8.
2002: Peter Mayr; Bernd Nidetzky
Catalytic reaction profile for NADH-dependent reduction of aromatic aldehydes by xylose reductase from Candida tenuis.
The Biochemical journal 2002;366(Pt 3):889-99.
2002: Mario Klimacek; Bernd Nidetzky
Examining the relative timing of hydrogen abstraction steps during NAD(+)-dependent oxidation of secondary alcohols catalyzed by long-chain D-mannitol dehydrogenase from Pseudomonas fluorescens using pH and kinetic isotope effects.
Biochemistry 2002;41(31):10158-65.
2002: Kathryn L Kavanagh; Mario Klimacek; Bernd Nidetzky; David K Wilson
The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis.
Biochemistry 2002;41(28):8785-95.
2002: Christian Eis; Bernd Nidetzky
Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors.
The Biochemical journal 2002;363(Pt 2):335-40.
2002: Inge Petzelbauer; Bernhard Kuhn; Barbara Splechtna; Klaus D Kulbe; Bernd Nidetzky
Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable beta-glycosidases and optimization of a packed-bed reactor for lactose conversion.
Biotechnology and bioengineering 2002;77(6):619-31.
2002: Inge Petzelbauer; Barbara Splechtna; Bernd Nidetzky
Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable beta-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions.
Biotechnology and bioengineering 2002;77(4):394-404.
2002: Gerhard Mayer; Klaus D Kulbe; Bernd Nidetzky
Utilization of xylitol dehydrogenase in a combined microbial/enzymatic process for production of xylitol from D-glucose.
Applied biochemistry and biotechnology 2002;98-100():577-89.
2002: Christian Leitner; Johann Hess; Christiane Galhaup; Roland Ludwig; Bernd Nidetzky; Klaus D Kulbe; Dietmar Haltrich
Purification and characterization of a laccase from the white-rot fungus Trametes multicolor.
Applied biochemistry and biotechnology 2002;98-100():497-507.
2002: Barbara Splechtna; Inge Petzelbauer; Bernhard Kuhn; Klaus D Kulbe; Bernd Nidetzky
Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk.
Applied biochemistry and biotechnology 2002;98-100():473-88.
2001: Bernd Nidetzky; Christian Eis
Alpha-retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors.
The Biochemical journal 2001;360(Pt 3):727-36.
2001: Inge Petzelbauer; Barbara Splechtna; Bernd Nidetzky
Galactosyl transfer catalyzed by thermostable beta-glycosidases from Sulfolobus solfataricus and Pyrococcus furiosus: kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles.
Journal of biochemistry 2001;130(3):341-9.
2001: Bernd Nidetzky; Mario Klimacek; Peter Mayr
Transient-state and steady-state kinetic studies of the mechanism of NADH-dependent aldehyde reduction catalyzed by xylose reductase from the yeast Candida tenuis.
Biochemistry 2001;40(34):10371-81.
2001: Mario Klimacek; M Szekely; Richard Griessler; Bernd Nidetzky
Exploring the active site of yeast xylose reductase by site-directed mutagenesis of sequence motifs characteristic of two dehydrogenase/reductase family types.
FEBS letters 2001;500(3):149-52.
2001: Christian Eis; M Watkins; Thomas Prohaska; Bernd Nidetzky
Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune.
The Biochemical journal 2001;356(Pt 3):757-67.
2001: Bernd Nidetzky; Peter Mayr; W Neuhauser; M Puchberger
Structural and functional properties of aldose xylose reductase from the D-xylose-metabolizing yeast Candida tenuis.
Chemico-biological interactions 2001;130-132(1-3):583-95.
2000: Bernd Nidetzky; Christian Eis; M Albert
Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda.
The Biochemical journal 2000;351 Pt 3():649-59.
2000: Inge Petzelbauer; A Reiter; Barbara Splechtna; kosma paul; Bernd Nidetzky
Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides during lactose conversion.
European journal of biochemistry / FEBS 2000;267(16):5055-66.
2000: Inge Petzelbauer; Reinhard Zeleny; A Reiter; Klaus D Kulbe; Bernd Nidetzky
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose: II. Oligosaccharide formation by two thermostable beta-glycosidases.
Biotechnology and bioengineering 2000;69(2):140-9.
2000: Richard Griessler; Sabato D'Auria; Fabio Tanfani; Bernd Nidetzky
Thermal denaturation pathway of starch phosphorylase from Corynebacterium callunae: oxyanion binding provides the glue that efficiently stabilizes the dimer structure of the protein.
Protein science : a publication of the Protein Society 2000;9(6):1149-61.
2000: Richard Griessler; Sabato D'Auria; R Schinzel; Fabio Tanfani; Bernd Nidetzky
Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli.
The Biochemical journal 2000;346 Pt 2():255-63.
2000: Peter Mayr; K Brüggler; Klaus D Kulbe; Bernd Nidetzky
D-Xylose metabolism by Candida intermedia: isolation and characterisation of two forms of aldose reductase with different coenzyme specificities.
Journal of chromatography. B, Biomedical sciences and applications 2000;737(1-2):195-202.
1999: Bernd Nidetzky; Peter Mayr; P Hadwiger; Arnold E Stütz
Binding energy and specificity in the catalytic mechanism of yeast aldose reductases.
The Biochemical journal 1999;344 Pt 1():101-7.
1999: Michael Graninger; Bernd Nidetzky; DE Heinrichs; Chris Whitfield; Paul Messner
Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar Typhimurium LT2.
The Journal of biological chemistry 1999;274(35):25069-77.
1999: M Slatner; Bernd Nidetzky; Klaus D Kulbe
Kinetic study of the catalytic mechanism of mannitol dehydrogenase from Pseudomonas fluorescens.
Biochemistry 1999;38(32):10489-98.
1999: Inge Petzelbauer; Bernd Nidetzky; Dietmar Haltrich; Klaus D Kulbe
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable beta-glycosidases.
Biotechnology and bioengineering 1999;64(3):322-32.
1999: Christian Eis; Bernd Nidetzky
Characterization of trehalose phosphorylase from Schizophyllum commune.
The Biochemical journal 1999;341 ( Pt 2)():385-93.
1999: P Hadwiger; Peter Mayr; A Tauss; Arnold E Stütz; Bernd Nidetzky
Binding and catalysis by yeast aldose reductase: a substrate-analog approach with new aldose derivatives.
Bioorganic & medicinal chemistry letters 1999;9(12):1683-6.
1999: U Baminger; Bernd Nidetzky; Klaus D Kulbe; Dietmar Haltrich
A simple assay for measuring cellobiose dehydrogenase activity in the presence of laccase.
Journal of microbiological methods 1999;35(3):253-9.
1998: M Slatner; G Nagl; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
Enzymatic synthesis of mannitol. Reaction engineering for a recombinant mannitol dehydrogenase.
Annals of the New York Academy of Sciences 1998;864():450-3.
1998: Bernd Nidetzky; W Neuhauser; Peter Mayr; Dietmar Haltrich; Klaus D Kulbe
Strategies to an efficient enzymatic production of xylitol.
Annals of the New York Academy of Sciences 1998;864():442-5.
1998: Dietmar Haltrich; Christian Leitner; W Neuhauser; Bernd Nidetzky; Klaus D Kulbe; Jindrich Volc
A convenient enzymatic procedure for the production of aldose-free D-tagatose.
Annals of the New York Academy of Sciences 1998;864():295-9.
1998: Christian Eis; M Albert; K Dax; Bernd Nidetzky
The stereochemical course of the reaction mechanism of trehalose phosphorylase from Schizophyllum commune.
FEBS letters 1998;440(3):440-3.
1998: R Lunzer; Y Mamnun; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases.
The Biochemical journal 1998;336 ( Pt 1)():91-9.
1998: W Neuhauser; M Steininger; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
A pH-controlled fed-batch process can overcome inhibition by formate in NADH-dependent enzymatic reductions using formate dehydrogenase-catalyzed coenzyme regeneration.
Biotechnology and bioengineering 1998;60(3):277-82.
1998: M Fürlinger; M Satory; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
Control of the association state of tetrameric glucose-fructose oxidoreductase from Zymomonas mobilis as the rationale for stabilization of the enzyme in biochemical reactors.
Journal of biochemistry 1998;124(2):280-6.
1998: A Sachslehner; Bernd Nidetzky; Klaus D Kulbe; Dietmar Haltrich
Induction of Mannanase, Xylanase, and Endoglucanase Activities in Sclerotium rolfsii.
Applied and environmental microbiology 1998;64(2):594-600.
1998: M Fürlinger; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
A multistep process is responsible for product-induced inactivation of glucose-fructose oxidoreductase from Zymomonas mobilis.
European journal of biochemistry / FEBS 1998;251(3):955-63.
1998: W Neuhauser; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
Noncovalent enzyme-substrate interactions in the catalytic mechanism of yeast aldose reductase.
Biochemistry 1998;37(4):1116-23.
1998: M Silva-Martinez; Dietmar Haltrich; S Novalic; Klaus D Kulbe; Bernd Nidetzky
Simultaneous enzymatic synthesis of gluconic acid and sorbitol. Continuous process development using glucose-fructose oxidoreductase from Zymomonas mobilis.
Applied biochemistry and biotechnology 1998;70-72():863-8.
1998: A Sachslehner; Dietmar Haltrich; G Gübitz; Bernd Nidetzky; Klaus D Kulbe
Efficient production of mannan-degrading enzymes by the basidiomycete Sclerotium rolfsii.
Applied biochemistry and biotechnology 1998;70-72():939-53.
1998: Christian Leitner; Dietmar Haltrich; Bernd Nidetzky; Hansjörg Prillinger; Klaus D Kulbe
Production of a novel pyranose 2-oxidase by basidiomycete Trametes multicolor.
Applied biochemistry and biotechnology 1998;70-72():237-48.
1997: Christian Eis; Richard Griessler; M Maier; Andreas Weinhaeusel; B Bock; Klaus D Kulbe; Dietmar Haltrich; R Schinzel; Bernd Nidetzky
Efficient downstream processing of maltodextrin phosphorylase from Escherichia coli and stabilization of the enzyme by immobilization onto hydroxyapatite.
Journal of biotechnology 1997;58(3):157-66.
1997: Andreas Weinhaeusel; Richard Griessler; Angelika Krebs; Peter Zipper; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
alpha-1,4-D-glucan phosphorylase of gram-positive Corynebacterium callunae: isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering.
The Biochemical journal 1997;326 ( Pt 3)():773-83.
1997: W Neuhauser; Dietmar Haltrich; Klaus D Kulbe; Bernd Nidetzky
NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation, characterization and biochemical properties of the enzyme.
The Biochemical journal 1997;326 ( Pt 3)():683-92.
1997: M Kern; Dietmar Haltrich; Bernd Nidetzky; Klaus D Kulbe
Induction of aldose reductase and xylitol dehydrogenase activities in Candida tenuis CBS 4435.
FEMS microbiology letters 1997;149(1):31-7.
1997: Bernd Nidetzky; M Fürlinger; D Gollhofer; R K Scopes; Dietmar Haltrich; Klaus D Kulbe
Improved operational stability of cell-free glucose-fructose oxidoreductase from Zymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor.
Biotechnology and bioengineering 1997;53(6):623-9.
1997: A Sachslehner; Dietmar Haltrich; Bernd Nidetzky; Klaus D Kulbe
Production of hemicellulose- and cellulose-degrading enzymes by various strains of Sclerotium rolfsii.
Applied biochemistry and biotechnology 1997;63-65():189-201.
1997: Bernd Nidetzky; M Fürlinger; D Gollhofer; I Haug; Dietmar Haltrich; Klaus D Kulbe
Simultaneous enzymatic synthesis of gluconic acid and sorbitol: production, purification, and application of glucose-fructose oxidoreductase and gluconolactonase.
Applied biochemistry and biotechnology 1997;63-65():173-88.
1997: Bernd Nidetzky; Richard Griessler; Andreas Weinhaeusel; Dietmar Haltrich; Klaus D Kulbe
Reaction engineering aspects of alpha-l,4-D-glucan phosphorylase catalysis : comparison of plant and bacterial enzymes for the continuous synthesis of D-glucose-1-phosphate.
Applied biochemistry and biotechnology 1997;63-65():159-72.
1996: Bernd Nidetzky; W Neuhauser; Dietmar Haltrich; Klaus D Kulbe
Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor.
Biotechnology and bioengineering 1996;52(3):387-96.
1996: Bernd Nidetzky; Andreas Weinhaeusel; Dietmar Haltrich; Klaus D Kulbe; Reinhard Schinzel
Maltodextrin phosphorylase from Escherichia coli: production and application for the synthesis of alpha-glucose-1-phosphate.
Annals of the New York Academy of Sciences 1996;782():208-18.
1994: Bernd Nidetzky; W Steiner; Marc Claeyssens
Cellulose hydrolysis by the cellulases from Trichoderma reesei: adsorptions of two cellobiohydrolases, two endocellulases and their core proteins on filter paper and their relation to hydrolysis.
The Biochemical journal 1994;303 ( Pt 3)():817-23.
1994: Bernd Nidetzky; W Steiner; Marianne Hayn; Marc Claeyssens
Cellulose hydrolysis by the cellulases from Trichoderma reesei: a new model for synergistic interaction.
The Biochemical journal 1994;298 Pt 3():705-10.