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Roberto Nucci

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TOP 3
Margherita Pezzullo; Mosè Rossi; Pompea Del Vecchio; Luigi Mandrich; Roberto Nucci; Giuseppe Manco
Comprehensive analysis of surface charged residues involved in thermal stability in Alicyclobacillus acidocaldarius esterase 2.
Ferdinando Febbraio; Luigia Merone; Roberto Nucci; Mosè Rossi; Giovanni Paolo Cetrangolo; Giuseppe Manco
Thermostable esterase 2 from Alicyclobacillus acidocaldarius as biosensor for the detection of organophosphate pesticides.
Giuseppe Manco; Roberto Nucci; Ferdinando Febbraio
Use of esterase activities for the detection of chemical neurotoxic agents.

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All Publications

2013: Margherita Pezzullo; Mosè Rossi; Pompea Del Vecchio; Luigi Mandrich; Roberto Nucci; Giuseppe Manco
Comprehensive analysis of surface charged residues involved in thermal stability in Alicyclobacillus acidocaldarius esterase 2.
Protein engineering, design & selection : PEDS 2013;26(1):47-58.
2011: Ferdinando Febbraio; Luigia Merone; Roberto Nucci; Mosè Rossi; Giovanni Paolo Cetrangolo; Giuseppe Manco
Thermostable esterase 2 from Alicyclobacillus acidocaldarius as biosensor for the detection of organophosphate pesticides.
Analytical chemistry 2011;83(5):1530-6.
2009: Giuseppe Manco; Roberto Nucci; Ferdinando Febbraio
Use of esterase activities for the detection of chemical neurotoxic agents.
Protein and peptide letters 2009;16(10):1225-34.
2007: Anna Maria Salzano; Ferdinando Febbraio; Tiziana Farias; Giovanni P Cetrangolo; Roberto Nucci; Andrea Scaloni; Giuseppe Manco
Redox stress proteins are involved in adaptation response of the hyperthermoacidophilic archaeon Sulfolobus solfataricus to nickel challenge.
Microbial cell factories 2007;6():25.
2006: Adele Cutignano; Giuliana D'Ippolito; Giovanna Romano; Nadia Lamari; Guido Cimino; Ferdinando Febbraio; Roberto Nucci; Angelo Fontana
Chloroplastic glycolipids fuel aldehyde biosynthesis in the marine diatom Thalassiosira rotula.
Chembiochem : a European journal of chemical biology 2006;7(3):450-6.
2004: Raffaella Briante; Ferdinando Febbraio; Roberto Nucci
Antioxidant/prooxidant effects of dietary non-flavonoid phenols on the Cu2+-induced oxidation of human low-density lipoprotein (LDL).
Chemistry & biodiversity 2004;1(11):1716-29.
2004: Raffaella Briante; Maurizio Patumi; Ferdinando Febbraio; Roberto Nucci
Production of highly purified hydroxytyrosol from Olea europaea leaf extract biotransformed by hyperthermophilic beta-glycosidase.
Journal of biotechnology 2004;111(1):67-77.
2004: Ferdinando Febbraio; Annapaola Andolfo; Fabio Tanfani; Raffaella Briante; Fabrizio Gentile; Silvestro Formisano; Carlo Vaccaro; andrea scirè; Enrico Bertoli; Piero Pucci; Roberto Nucci
Thermal stability and aggregation of sulfolobus solfataricus beta-glycosidase are dependent upon the N-epsilon-methylation of specific lysyl residues: critical role of in vivo post-translational modifications.
The Journal of biological chemistry 2004;279(11):10185-94.
2004: Ettore Bismuto; Roberto Nucci; Ferdinando Febbraio; Fabio Tanfani; Fabrizio Gentile; Raffaella Briante; andrea scirè; Enrico Bertoli; Pietro Amodeo
Effects induced by mono- and divalent cations on protein regions responsible for thermal adaptation in beta-glycosidase from Sulfolobus solfataricus.
European biophysics journal : EBJ 2004;33(1):38-49.
2003: Raffaella Briante; Ferdinando Febbraio; Roberto Nucci
Antioxidant properties of low molecular weight phenols present in the mediterranean diet.
Journal of agricultural and food chemistry 2003;51(24):6975-81.
2003: Ettore Bismuto; Ferdinando Febbraio; Simona Limongelli; Raffaella Briante; Roberto Nucci
Dynamic fluorescence studies of beta-glycosidase mutants from Sulfolobus solfataricus: effects of single mutations on protein thermostability.
Proteins 2003;51(1):10-20.
2002: Fabrizio Gentile; Pietro Amodeo; Ferdinando Febbraio; Francesco Picaro; Andrea Motta; Silvestro Formisano; Roberto Nucci
SDS-resistant active and thermostable dimers are obtained from the dissociation of homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus in SDS. Stabilizing role of the A-C intermonomeric interface.
The Journal of biological chemistry 2002;277(46):44050-60.
2002: Evgenia Lozinsky; Ferdinando Febbraio; Alexander I Shames; Gertz I Likhtenshtein; Ettore Bismuto; Roberto Nucci
Heterogeneity in the structural dynamics of Sulfolobus solfataricus beta-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry.
Protein science : a publication of the Protein Society 2002;11(11):2535-44.
2002: Raffaella Briante; Maurizio Patumi; Stefano Terenziani; Ettore Bismuto; Ferdinando Febbraio; Roberto Nucci
Olea europaea L. leaf extract and derivatives: antioxidant properties.
Journal of agricultural and food chemistry 2002;50(17):4934-40.
2002: Raffaella Briante; Francesco La Cara; Ferdinando Febbraio; Maurizio Patumi; Roberto Nucci
Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extracts.
Journal of biotechnology 2002;93(2):109-19.
2001: Raffaella Briante; Francesco La Cara; M P Tonziello; Ferdinando Febbraio; Roberto Nucci
Antioxidant activity of the main bioactive derivatives from oleuropein hydrolysis by hyperthermophilic beta-glycosidase.
Journal of agricultural and food chemistry 2001;49(7):3198-203.
2000: Gertz I Likhtenshtein; Ferdinando Febbraio; Roberto Nucci
Intramolecular dynamics and conformational transition in proteins studied by biophysical labelling methods. Common and specific features of proteins from thermophylic micro-organisms.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2000;56A(10):2011-31.
2000: Raffaella Briante; Francesco La Cara; Ferdinando Febbraio; Roberto Barone; gennaro Piccialli; R Carolla; P Mainolfi; Lorenzo De Napoli; Maurizio Patumi; G Fontanazza; Roberto Nucci
Hydrolysis of oleuropein by recombinant beta-glycosidase from hyperthermophilic archaeon Sulfolobus solfataricus immobilised on chitosan matrix.
Journal of biotechnology 2000;77(2-3):275-86.
1999: Sabato D'Auria; Roberto Nucci; Mosè Rossi; Ignacy Gryczynski; Zygmunt Gryczynski; Joseph R Lakowicz
The beta-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: enzyme activity and conformational dynamics at temperatures above 100 degrees C.
Biophysical chemistry 1999;81(1):23-31.
1999: Sabato D'Auria; Roberto Nucci; Mosè Rossi; Enrico Bertoli; Fabio Tanfani; Ignacy Gryczynski; Henryk Malak; Joseph R Lakowicz
beta-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: structure and activity in the presence of alcohols.
Journal of biochemistry 1999;126(3):545-52.
1999: Roberto Barone; Raffaella Briante; Sabato D'Auria; Ferdinando Febbraio; C Vaccaro; Luigi Del Giudice; G M Borrelli; N Di Fonzo; Roberto Nucci
Purification and characterization of a lipoxygenase enzyme from durum wheat semolina.
Journal of agricultural and food chemistry 1999;47(5):1924-31.
1999: Ettore Bismuto; Roberto Nucci; M Rossi; Gaetano Irace
Structural and dynamic aspects of beta-glycosidase from mesophilic and thermophilic bacteria by multitryptophanyl emission decay studies.
Proteins 1999;35(2):163-72.
1998: Sabato D'Auria; Marco Moracci; Ferdinando Febbraio; Fabio Tanfani; Roberto Nucci; Mosè Rossi
Structure-function studies on beta-glycosidase from Sulfolobus solfataricus. Molecular bases of thermostability.
Biochimie 1998;80(11):949-57.
1998: Francesca Catanzano; Giuseppe Graziano; Barbara de Paola; Guido Barone; Sabato D'Auria; M Rossi; Roberto Nucci
Guanidine-induced denaturation of beta-glycosidase from Sulfolobus solfataricus expressed in Escherichia coli.
Biochemistry 1998;37(41):14484-90.
1998: Ferdinando Febbraio; Marianna Portaccio; S Stellato; Sergio Rossi; Umberto Bencivenga; Roberto Nucci; M Rossi; Francesco Saverio Gaeta; Damiano Gustavo Mita
Advantages in using immobilized thermophilic beta-glycosidase in nonisothermal bioreactors.
Biotechnology and bioengineering 1998;59(1):108-15.
1998: Carlo Fini; Marcello Coli; Ardesio Floridi; Sabato D'Auria; Maria Staiano; Roberto Nucci; Mose' Rossi
Temperature effects on the structural and functional properties of GPI-anchored and anchor-less bull seminal plasma ecto-5'-nucleotidase.
Journal of biochemistry 1998;123(2):269-74.
1997: C F Aguilar; I Sanderson; Marco Moracci; Maria Ciaramella; Roberto Nucci; Mose' Rossi; Laurence H Pearl
Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability.
Journal of molecular biology 1997;271(5):789-802.
1997: Sabato D'Auria; Roberto Barone; Mosè Rossi; Roberto Nucci; Guido Barone; Dimitrios Fessas; Enrico Bertoli; Fabio Tanfani
Effects of temperature and SDS on the structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus.
The Biochemical journal 1997;323 ( Pt 3)():833-40.
1997: Ferdinando Febbraio; Roberto Barone; Sabato D'Auria; Mosè Rossi; Roberto Nucci; gennaro Piccialli; Lorenzo De Napoli; S Orrù; Piero Pucci
Identification of the active site nucleophile in the thermostable beta-glycosidase from the archaeon Sulfolobus solfataricus expressed in Escherichia coli.
Biochemistry 1997;36(11):3068-75.
1997: Ettore Bismuto; Gaetano Irace; Sabato D'Auria; M Rossi; Roberto Nucci
Multitryptophan-fluorescence-emission decay of beta-glycosidase from the extremely thermophilic archaeon Sulfolobus solfataricus.
European journal of biochemistry / FEBS 1997;244(1):53-8.
1997: Sabato D'Auria; Mosè Rossi; Roberto Nucci; Gaetano Irace; Ettore Bismuto
Perturbation of conformational dynamics, enzymatic activity, and thermostability of beta-glycosidase from archaeon Sulfolobus solfataricus by pH and sodium dodecyl sulfate detergent.
Proteins 1997;27(1):71-9.
1996: Sabato D'Auria; F Pellino; F La Cara; Roberto Barone; M Rossi; Roberto Nucci
Immobilization on chitosan of a thermophilic beta-glycosidase expressed in Saccharomyces cerevisiae.
Applied biochemistry and biotechnology 1996;61(1-2):157-66.
1996: Sabato D'Auria; M Rossi; Guido Barone; Francesca Catanzano; P Del Vecchio; Giuseppe Graziano; Roberto Nucci
Temperature-induced denaturation of beta-glycosidase from the archaeon Sulfolobus solfataricus.
Journal of biochemistry 1996;120(2):292-300.
1996: Sabato D'Auria; A Morana; Ferdinando Febbraio; C Vaccaro; M De Rosa; Roberto Nucci
Functional and structural properties of the homogeneous beta-glycosidase from the extreme thermoacidophilic archaeon sulfolobus solfataricus expressed in Saccharomyces cerevisiae.
Protein expression and purification 1996;7(3):299-308.
1995: Marco Moracci; Roberto Nucci; Ferdinando Febbraio; C Vaccaro; N Vespa; F La Cara; M Rossi
Expression and extensive characterization of a beta-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus in Escherichia coli: authenticity of the recombinant enzyme.
Enzyme and microbial technology 1995;17(11):992-7.
1994: Roberto Nucci; Ferdinando Febbraio; gennaro Piccialli; Lorenzo De Napoli; C Vaccaro; M Rossi; E P Whitehead
Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase.
Archives of biochemistry and biophysics 1994;310(1):49-53.
1993: Roberto Nucci; Marco Moracci; C Vaccaro; N Vespa; M Rossi
Exo-glucosidase activity and substrate specificity of the beta-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus.
Biotechnology and applied biochemistry 1993;17 ( Pt 2)():239-50.
1993: Laurence H Pearl; A M Hemmings; Roberto Nucci; M Rossi
Crystallization and preliminary X-ray analysis of the beta-galactosidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.
Journal of molecular biology 1993;229(2):561-3.
1991: Roberto Nucci; Carlo Antonio Raia; C Vaccaro; M Rossi; E P Whitehead
Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12)
Archives of biochemistry and biophysics 1991;289(1):19-25.
1991: E P Whitehead; Roberto Nucci; C Vaccaro; M Rossi
Hill coefficient ratios give binding ratios of allosteric enzyme effectors; inhibition, activation, and squatting in deoxycytidylate aminohydrolase (EC 3.5.4.12)
Archives of biochemistry and biophysics 1991;289(1):12-8.
1990: R Rella; Carlo Antonio Raia; F M Pisani; Sabato D'Auria; Roberto Nucci; A Gambacorta; M De Rosa; M Rossi
Purification and properties of a thermophilic and thermostable DNA polymerase from the archaebacterium Sulfolobus solfataricus.
The Italian journal of biochemistry 1990;39(2):83-99.
1990: F M Pisani; R Rella; Carlo Antonio Raia; C Rozzo; Roberto Nucci; A Gambacorta; M De Rosa; M Rossi
Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties.
European journal of biochemistry / FEBS 1990;187(2):321-8.
1986: Richard L Momparler; M Rossi; J Bouchard; Simonetta Bartolucci; Louise F Momparler; Carlo Antonio Raia; Roberto Nucci; C Vaccaro; S Sepe
5-AZA-2'-deoxycytidine synergistic action with thymidine on leukemic cells and interaction of 5-AZA-dCMP with dCMP deaminase.
Advances in experimental medicine and biology 1986;195 Pt B():157-63.
1983: S Mastrantonio; Roberto Nucci; C Vaccaro; M Rossi; E P Whitehead
Analysis of competition for substrate sites in an allosteric enzyme with co-operative kinetics. Effects of dAMP and dUMP on donkey spleen deoxycytidylate aminohydrolase.
European journal of biochemistry / FEBS 1983;137(3):421-7.
1982: Carlo Antonio Raia; Roberto Nucci; C Vaccaro; S Sepe; R Rella; M Rossi
Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form.
Journal of molecular biology 1982;157(3):557-70.