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Per-Ake Nygren
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44
Uhlén, Mathias
21
Ståhl, Stefan
9
Gräslund, Torbjörn
6
Hansson, Marianne
6
Gunneriusson, Elin
6
Hober, Sophia
5
Nord, Olof
5
Berzins, Klavs
5
Sjölander, A
4
Rönnmark, Jenny
4
Samuelson, Patrik
4
Bandmann, Nina
4
Nord, Karin
4
Jonasson, Per
3
Lendel, Christofer
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All Publications
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2009: Vernet Erik; Lundberg Emma; Friedman Mikaela; Rigamonti Nicolò; Klausing Sandra; Nygren Per-Ake; Gräslund Torbjörn
Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain.
New biotechnology 2009;25(6):417-23.
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2008: Vernet Erik; Konrad Anna; Lundberg Emma; Nygren Per-Ake; Gräslund Torbjörn
Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule.
Journal of immunological methods 2008;338(1-2):1-6.
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2008: Nygren Per-Ake
Alternative binding proteins: affibody binding proteins developed from a small three-helix bundle scaffold.
The FEBS journal 2008;275(11):2668-76.
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2007: Wagner Samuel; Baars Louise; Ytterberg A Jimmy; Klussmeier Anja; Wagner Claudia S; Nord Olof; Nygren Per-Ake; van Wijk Klaas J; de Gier Jan-Willem
Consequences of membrane protein overexpression in Escherichia coli.
Molecular & cellular proteomics : MCP 2007;6(9):1527-50.
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2007: Myhre S; Henning P; Granio O; Tylö A S; Nygren P A; Olofsson S; Boulanger P; Lindholm L; Hong S-S
Decreased immune reactivity towards a knobless, affibody-targeted adenovirus type 5 vector.
Gene therapy 2007;14(4):376-81.
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2007: Renberg Björn; Nordin Jon; Merca Anna; Uhlén Mathias; Feldwisch Joachim; Nygren Per-Ake; Karlström Amelie Eriksson
Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats.
Journal of proteome research 2007;6(1):171-9.
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2007: Bandmann Nina; Nygren Per-Ake
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli.
Nucleic acids research 2007;35(5):e32.
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2005: Engfeldt Torun; Renberg Björn; Brumer Harry; Nygren Per Ake; Karlström Amelie Eriksson
Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein.
Chembiochem : a European journal of chemical biology 2005;6(6):1043-50.
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2005: Wiklund Martin; Nord Olof; Gothäll Rikard; Chernyshev Andrei V; Nygren Per-Ake; Hertz Hans M
Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay.
Analytical biochemistry 2005;338(1):90-101.
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2005: Henning P; Andersson K M E; Frykholm K; Ali A; Magnusson M K; Nygren P-A; Granio O; Hong S S; Boulanger P; Lindholm L
Tumor cell targeted gene delivery by adenovirus 5 vectors carrying knobless fibers with antibody-binding domains.
Gene therapy 2005;12(3):211-24.
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2005: Nord Olof; Gustrin Anna; Nygren Per-Ake
Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation.
FEMS microbiology letters 2005;242(1):73-9.
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2004: Lendel Christofer; Dincbas-Renqvist Vildan; Flores Alexander; Wahlberg Elisabet; Dogan Jakob; Nygren Per-Ake; Härd Torleif
Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A.
Protein science : a publication of the Protein Society 2004;13(8):2078-88.
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2004: Nygren Per-Ake; Skerra Arne
Binding proteins from alternative scaffolds.
Journal of immunological methods 2004;290(1-2):3-28.
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2004: Eklund Malin; Sandström Kristofer; Teeri Tuula T; Nygren Per-Ake
Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex.
Journal of biotechnology 2004;109(3):277-86.
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2003: Linhult Martin; Gülich Susanne; Gräslund Torbjörn; Nygren Per-Ake; Hober Sophia
Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand.
Protein engineering 2003;16(12):1147-52.
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2003: Andersson Mårten; Rönnmark Jenny; Areström Iréne; Nygren Per Ake; Ahlborg Niklas
Inclusion of a non-immunoglobulin binding protein in two-site ELISA for quantification of human serum proteins without interference by heterophilic serum antibodies.
Journal of immunological methods 2003;283(1-2):225-34.
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2003: Nord Olof; Uhlén Mathias; Nygren Per-Ake
Microbead display of proteins by cell-free expression of anchored DNA.
Journal of biotechnology 2003;106(1):1-13.
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2003: Rönnmark Jenny; Kampf Caroline; Asplund Anna; Höidén-Guthenberg Ingmarie; Wester Kenneth; Pontén Fredrik; Uhlén Mathias; Nygren Per-Ake
Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol.
Journal of immunological methods 2003;281(1-2):149-60.
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2003: Sandström K; Xu Z; Forsberg G; Nygren P-A
Inhibition of the CD28-CD80 co-stimulation signal by a CD28-binding affibody ligand developed by combinatorial protein engineering.
Protein engineering 2003;16(9):691-7.
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2003: Högbom Martin; Eklund Malin; Nygren Per-Ake; Nordlund Pär
Structural basis for recognition by an in vitro evolved affibody.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(6):3191-6.
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2003: Wahlberg Elisabet; Lendel Christofer; Helgstrand Magnus; Allard Peter; Dincbas-Renqvist Vildan; Hedqvist Anders; Berglund Helena; Nygren Per-Ake; Härd Torleif
An affibody in complex with a target protein: structure and coupled folding.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(6):3185-90.
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2002: Lendel Christofer; Wahlberg Elisabet; Berglund Helena; Eklund Malin; Nygren Per-Ake; Härd Torleif
1H, 13C and 15N resonance assignments of an affibody-target complex.
Journal of biomolecular NMR 2002;24(3):271-2.
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2002: Gräslund Susanne; Eklund Malin; Falk Ronny; Uhlén Mathias; Nygren Per-Ake; Ståhl Stefan
A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products.
Journal of biotechnology 2002;99(1):41-50.
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2002: Henning P; Magnusson M K; Gunneriusson E; Hong S S; Boulanger P; Nygren P-A; Lindholm L
Genetic modification of adenovirus 5 tropism by a novel class of ligands based on a three-helix bundle scaffold derived from staphylococcal protein A.
Human gene therapy 2002;13(12):1427-39.
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2002: Eklund Malin; Axelsson Lars; Uhlén Mathias; Nygren Per-Ake
Anti-idiotypic protein domains selected from protein A-based affibody libraries.
Proteins 2002;48(3):454-62.
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2002: Gräslund Torbjörn; Hedhammar My; Uhlén Mathias; Nygren Per Ake; Hober Sophia
Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology.
Journal of biotechnology 2002;96(1):93-102.
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2002: Rönnmark Jenny; Grönlund Hans; Uhlén Mathias; Nygren Per-Ake
Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A.
European journal of biochemistry / FEBS 2002;269(11):2647-55.
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2002: Samuelson Patrik; Gunneriusson Elin; Nygren Per Ake; Ståhl Stefan
Display of proteins on bacteria.
Journal of biotechnology 2002;96(2):129-54.
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2002: Jonasson Per; Liljeqvist Sissela; Nygren Per-Ake; Ståhl Stefan
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.
Biotechnology and applied biochemistry 2002;35(Pt 2):91-105.
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2002: Rönnmark Jenny; Hansson Marianne; Nguyen Thien; Uhlén Mathias; Robert Alain; Ståhl Stefan; Nygren Per-Ake
Construction and characterization of affibody-Fc chimeras produced in Escherichia coli.
Journal of immunological methods 2002;261(1-2):199-211.
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2002: Johansson Maria U; Frick Inga-Maria; Nilsson Hanna; Kraulis Per J; Hober Sophia; Jonasson Per; Linhult Martin; Nygren Per-Ake; Uhlén Mathias; Björck Lars; Drakenberg Torbjörn; Forsén Sture; Wikström Mats
Structure, specificity, and mode of interaction for bacterial albumin-binding modules.
The Journal of biological chemistry 2002;277(10):8114-20.
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2002: Gräslund Torbjörn; Ehn Maria; Lundin Gunnel; Hedhammar My; Uhlén Mathias; Nygren Per-Ake; Hober Sophia
Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner.
Journal of chromatography. A 2002;942(1-2):157-66.
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2002: Bandmann Nina; Van Alstine James; Veide Andres; Nygren Per-Ake
Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins.
Journal of biotechnology 2002;93(1):1-14.
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2001: Wernérus H; Lehtiö J; Teeri T; Nygren P A; Ståhl S
Generation of metal-binding staphylococci through surface display of combinatorially engineered cellulose-binding domains.
Applied and environmental microbiology 2001;67(10):4678-84.
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2001: Nord K; Nord O; Uhlén M; Kelley B; Ljungqvist C; Nygren P A
Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A.
European journal of biochemistry / FEBS 2001;268(15):4269-77.
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2001: Karlström A; Nygren P A
Dual labeling of a binding protein allows for specific fluorescence detection of native protein.
Analytical biochemistry 2001;295(1):22-30.
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2001: Danielsen S; Eklund M; Deussen H J; Gräslund T; Nygren P A; Borchert T V
In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.
Gene 2001;272(1-2):267-74.
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2000: Lehtiö J; Teeri T T; Nygren P A
Alpha-amylase inhibitors selected from a combinatorial library of a cellulose binding domain scaffold.
Proteins 2000;41(3):316-22.
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2000: Gräslund T; Lundin G; Uhlén M; Nygren P A; Hober S
Charge engineering of a protein domain to allow efficient ion-exchange recovery.
Protein engineering 2000;13(10):703-9.
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2000: Hansson M; Nygren P A; Ståhl S
Design and production of recombinant subunit vaccines.
Biotechnology and applied biochemistry 2000;32 ( Pt 2)():95-107.
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2000: Berggren K; Nilsson A; Johansson G; Bandmann N; Nygren P A; Tjerneld F
Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure.
Journal of chromatography. B, Biomedical sciences and applications 2000;743(1-2):295-306.
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2000: Nord K; Gunneriusson E; Uhlén M; Nygren P A
Ligands selected from combinatorial libraries of protein A for use in affinity capture of apolipoprotein A-1M and taq DNA polymerase.
Journal of biotechnology 2000;80(1):45-54.
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2000: Bandmann N; Collet E; Leijen J; Uhlén M; Veide A; Nygren P A
Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems.
Journal of biotechnology 2000;79(2):161-72.
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2000: Legendre D; Laraki N; Gräslund T; Bjørnvad M E; Bouchet M; Nygren P A; Borchert T V; Fastrez J
Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.
Journal of molecular biology 2000;296(1):87-102.
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2000: Jonasson P; Nygren P A; Jörnvall H; Johansson B L; Wahren J; Uhlén M; Ståhl S
Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein.
Journal of biotechnology 2000;76(2-3):215-26.
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1999: Andersson K; Gülich S; Hämäläinen M; Nygren P A; Hober S; Malmqvist M
Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space.
Proteins 1999;37(3):494-8.
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1999: Gunneriusson E; Samuelson P; Ringdahl J; Grönlund H; Nygren P A; Ståhl S
Staphylococcal surface display of immunoglobulin A (IgA)- and IgE-specific in vitro-selected binding proteins (affibodies) based on Staphylococcus aureus protein A.
Applied and environmental microbiology 1999;65(9):4134-40.
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1999: Hansson M; Ringdahl J; Robert A; Power U; Goetsch L; Nguyen T N; Uhlén M; Ståhl S; Nygren P A
An in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (RSV) G protein.
Immunotechnology : an international journal of immunological engineering 1999;4(3-4):237-52.
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1999: Nilsson P; Larsson A; Lundeberg J; Uhlén M; Nygren P A
Mutational scanning of PCR products by subtractive oligonucleotide hybridization analysis.
BioTechniques 1999;26(2):308-16.
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1998: Jonasson P; Nygren P A; Johansson B L; Wahren J; Uhlén M; Ståhl S
Gene fragment polymerization gives increased yields of recombinant human proinsulin C-peptide.
Gene 1998;210(2):203-10.
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1998: Jansson B; Uhlén M; Nygren P A
All individual domains of staphylococcal protein A show Fab binding.
FEMS immunology and medical microbiology 1998;20(1):69-78.
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1998: O'Meara D; Nilsson P; Nygren P A; Uhlén M; Lundeberg J
Capture of single-stranded DNA assisted by oligonucleotide modules.
Analytical biochemistry 1998;255(2):195-203.
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1997: Nilsson J; Ståhl S; Lundeberg J; Uhlén M; Nygren P A
Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins.
Protein expression and purification 1997;11(1):1-16.
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1997: Nygren P A; Uhlén M
Scaffolds for engineering novel binding sites in proteins.
Current opinion in structural biology 1997;7(4):463-9.
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1997: Nord K; Gunneriusson E; Ringdahl J; Ståhl S; Uhlén M; Nygren P A
Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain.
Nature biotechnology 1997;15(8):772-7.
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1997: Ahlborg N; Sterky F; Haddad D; Perlmann P; Nygren P A; Andersson R; Berzins K
Predominance of H-2d- and H-2k-restricted T-cell epitopes in the highly repetitive Plasmodium falciparum antigen Pf332.
Molecular immunology 1997;34(5):379-89.
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1997: Nilsson J; Bosnes M; Larsen F; Nygren P A; Uhlén M; Lundeberg J
Heat-mediated activation of affinity-immobilized Taq DNA polymerase.
BioTechniques 1997;22(4):744-51.
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1997: Gräslund T; Nilsson J; Lindberg A M; Uhlén M; Nygren P A
Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein.
Protein expression and purification 1997;9(1):125-32.
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1997: Sjölander A; Nygren P A; Stahl S; Berzins K; Uhlen M; Perlmann P; Andersson R
The serum albumin-binding region of streptococcal protein G: a bacterial fusion partner with carrier-related properties.
Journal of immunological methods 1997;201(1):115-23.
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1997: Jendeberg L; Nilsson P; Larsson A; Denker P; Uhlén M; Nilsson B; Nygren P A
Engineering of Fc(1) and Fc(3) from human immunoglobulin G to analyse subclass specificity for staphylococcal protein A.
Journal of immunological methods 1997;201(1):25-34.
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1997: Nilsson P; Persson B; Larsson A; Uhlén M; Nygren P A
Detection of mutations in PCR products from clinical samples by surface plasmon resonance.
Journal of molecular recognition : JMR 1997;10(1):7-17.
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1997: Ståhl S; Nygren P A
The use of gene fusions to protein A and protein G in immunology and biotechnology.
Pathologie-biologie 1997;45(1):66-76.
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1997: Ståhl S; Nygren P A; Uhlén M
Detection and isolation of recombinant proteins based on binding affinity of reporter: protein A.
Methods in molecular biology (Clifton, N.J.) 1997;63():103-18.
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1997: Ståhl S; Nygren P A; Uhlén M
Strategies for gene fusions.
Methods in molecular biology (Clifton, N.J.) 1997;62():37-54.
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1996: Nilsson J; Larsson M; Ståhl S; Nygren P A; Uhlén M
Multiple affinity domains for the detection, purification and immobilization of recombinant proteins.
Journal of molecular recognition : JMR 1996;9(5-6):585-94.
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1996: Gunneriusson E; Samuelson P; Uhlen M; Nygren P A; Stähl S
Surface display of a functional single-chain Fv antibody on staphylococci.
Journal of bacteriology 1996;178(5):1341-6.
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1996: Kraulis P J; Jonasson P; Nygren P A; Uhlén M; Jendeberg L; Nilsson B; Kördel J
The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study.
FEBS letters 1996;378(2):190-4.
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1995: Nguyen T N; Gourdon M H; Hansson M; Robert A; Samuelson P; Libon C; Andréoni C; Nygren P A; Binz H; Uhlén M
Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus.
Journal of biotechnology 1995;42(3):207-19.
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1995: Nord K; Nilsson J; Nilsson B; Uhlén M; Nygren P A
A combinatorial library of an alpha-helical bacterial receptor domain.
Protein engineering 1995;8(6):601-8.
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1995: Karlsson R; Jendeberg L; Nilsson B; Nilsson J; Nygren P A
Direct and competitive kinetic analysis of the interaction between human IgG1 and a one domain analogue of protein A.
Journal of immunological methods 1995;183(1):43-9.
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1995: Nilsson P; Persson B; Uhlén M; Nygren P A
Real-time monitoring of DNA manipulations using biosensor technology.
Analytical biochemistry 1995;224(1):400-8.
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1994: Nilsson J; Nilsson P; Williams Y; Pettersson L; Uhlén M; Nygren P A
Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.
European journal of biochemistry / FEBS 1994;224(1):103-8.
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1994: Nygren P A; Ståhl S; Uhlén M
Engineering proteins to facilitate bioprocessing.
Trends in biotechnology 1994;12(5):184-8.
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1993: Ståhl S; Nygren P A; Sjölander A; Uhlén M
Engineered bacterial receptors in immunology.
Current opinion in immunology 1993;5(2):272-7.
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1992: Lorca T; Labbé J C; Devault A; Fesquet D; Strausfeld U; Nilsson J; Nygren P A; Uhlen M; Cavadore J C; Dorée M
Cyclin A-cdc2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs.
Journal of cell science 1992;102 ( Pt 1)():55-62.
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1991: Murby M; Cedergren L; Nilsson J; Nygren P A; Hammarberg B; Nilsson B; Enfors S O; Uhlén M
Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy.
Biotechnology and applied biochemistry 1991;14(3):336-46.
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1991: Eliasson M; Andersson R; Nygren P A; Uhlén M
Structural and functional analysis of the human IgG-Fab receptor activity of streptococcal protein G.
Molecular immunology 1991;28(10):1055-61.
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1991: Murby M; Nygren P A; Rondahl H; Hellman U; Enfors S O; Uhlén M
Differential degradation of a recombinant albumin-binding receptor in Escherichia coli.
European journal of biochemistry / FEBS 1991;199(1):41-6.
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1991: Sjölander A; Lövgren K; Ståhl S; Aslund L; Hansson M; Nygren P A; Larsson M; Hagstedt M; Wåhlin B; Berzins K
High antibody responses in rabbits immunized with influenza virus ISCOMs containing a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.
Vaccine 1991;9(6):443-50.
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1990: Nygren P A; Ljungquist C; Trømborg H; Nustad K; Uhlén M
Species-dependent binding of serum albumins to the streptococcal receptor protein G.
European journal of biochemistry / FEBS 1990;193(1):143-8.
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1990: Ståhl S; Sjölander A; Hansson M; Nygren P A; Uhlén M
A general strategy for polymerization, assembly and expression of epitope-carrying peptides applied to the Plasmodium falciparum antigen Pf155/RESA.
Gene 1990;89(2):187-93.
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1990: Sjölander A; Ståhl S; Nygren P A; Aslund L; Ahlborg N; Wåhlin B; Scherf A; Berzins K; Uhlén M; Perlmann P
Immunogenicity and antigenicity in rabbits of a repeated sequence of Plasmodium falciparum antigen Pf155/RESA fused to two immunoglobulin G-binding domains of staphylococcal protein A.
Infection and immunity 1990;58(4):854-9.
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1989: Ståhl S; Sjölander A; Nygren P A; Berzins K; Perlmann P; Uhlén M
A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.
Journal of immunological methods 1989;124(1):43-52.
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1989: Hammarberg B; Nygren P A; Holmgren E; Elmblad A; Tally M; Hellman U; Moks T; Uhlén M
Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(12):4367-71.
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1988: Nygren P A; Eliasson M; Abrahmsén L; Uhlén M; Palmcrantz E
Analysis and use of the serum albumin binding domains of streptococcal protein G.
Journal of molecular recognition : JMR 1988;1(2):69-74.
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