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Stephen Benkovic

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Yanhui Zhao; Joseph Rufo; Hongjun Yue; Stephen J Benkovic; Danqi Chen; Jarrod B French; Tony Jun Huang
Lab-on-a-chip technologies for single-molecule studies.
Hong Zhao; Ye Fang; Jarrod B French; Stephen J Benkovic
The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.
Mark Hedglin; Ravindra Kumar; Stephen J Benkovic
Replication clamps and clamp loaders.

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All Publications

2013: Yanhui Zhao; Joseph Rufo; Hongjun Yue; Stephen J Benkovic; Danqi Chen; Jarrod B French; Tony Jun Huang
Lab-on-a-chip technologies for single-molecule studies.
Lab on a chip 2013;13(12):2183-98.
2013: Hong Zhao; Ye Fang; Jarrod B French; Stephen J Benkovic
The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.
Chemical communications (Cambridge, England) 2013;49(40):4444-52.
2013: Mark Hedglin; Ravindra Kumar; Stephen J Benkovic
Replication clamps and clamp loaders.
Cold Spring Harbor perspectives in biology 2013;5(4):a010165.
2013: Mark Hedglin; Ravindra Kumar; Stephen J Benkovic
Replication clamps and clamp loaders.
Cold Spring Harbor perspectives in medicine 2013;3(3):a010165.
2013: Jarrod B French; Sherry Niessen; Hong Zhao; Songon AN; Benjamin F Cravatt; Yijun Deng; Stephen J Benkovic
Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome.
Proceedings of the National Academy of Sciences of the United States of America 2013;110(7):2528-33.
2013: Daniel Ahmed; Stephen J Benkovic; Chung Yu Chan; Sz-Chin Steven Lin; Hari S Muddana; Nitesh Nama; Tony Jun Huang
Tunable, pulsatile chemical gradient generation via acoustically driven oscillating bubbles.
Lab on a chip 2013;13(3):328-31.
2013: Senthil K Perumal; Wenhui Ren; Tae-Hee Lee; Stephen J Benkovic
How a holoenzyme for DNA replication is formed.
Proceedings of the National Academy of Sciences of the United States of America 2013;110(1):99-104.
2013: Stephen J Benkovic; Julie Theriot; Dagmar Ringe
Open questions - in brief: beyond -omics, missing motor proteins, and getting from molecules to organisms.
BMC biology 2013;11():8.
2012: John W Tomsho; Stephen J Benkovic
Examination of the reactivity of benzoxaboroles and related compounds with a cis-diol.
The Journal of organic chemistry 2012;77(24):11200-9.
2012: Maria Manosas; Senthil K Perumal; Vincent Croquette; Stephen J Benkovic
Direct observation of stalled fork restart via fork regression in the T4 replication system.
Science (New York, N.Y.) 2012;338(6111):1217-20.
2012: Shengxi Chen; Nour Eddine Fahmi; Stephen J Benkovic; Lin Wang; Sidney M Hecht
Two pyrenylalanines in dihydrofolate reductase form an excimer enabling the study of protein dynamics.
Journal of the American Chemical Society 2012;134(46):18883-5.
2012: Zhenxin Hu; Senthil K Perumal; Hongjun Yue; Stephen J Benkovic
The human lagging strand DNA polymerase δ holoenzyme is distributive.
The Journal of biological chemistry 2012;287(46):38442-8.
2012: Yijun Deng; Jarrod B French; Jongsik Gam; Hong Zhao; Songon AN; Stephen J Benkovic
Mapping protein-protein proximity in the purinosome.
The Journal of biological chemistry 2012;287(43):36201-7.
2012: Ian B Spurr; Stephen J Benkovic; Charles N Birts; Jeremy P Blaydes; Francesco Cuda; Ali Tavassoli
Targeting tumour proliferation with a small-molecule inhibitor of AICAR transformylase homodimerization.
Chembiochem : a European journal of chemical biology 2012;13(11):1628-34.
2012: Xiaoyun Ding; Stephen J Benkovic; I-Kao Chiang; Brian Kiraly; Sixing Li; Sz-Chin Steven Lin; Jinjie Shi; Hongjun Yue; Tony Jun Huang
On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(28):11105-9.
2012: Maria Manosas; Michelle M Spiering; Fangyuan Ding; Jean-François Allemand; Stephen J Benkovic; David Bensimon; Vincent Croquette
Mechanism of strand displacement synthesis by DNA replicative polymerases.
Nucleic acids research 2012;40(13):6174-86.
2012: Maria Manosas; Michelle M Spiering; Vincent Croquette; Fangyuan Ding; Stephen J Benkovic
Collaborative coupling between polymerase and helicase for leading-strand synthesis.
Nucleic acids research 2012;40(13):6187-98.
2012: Fangyuan Ding; Maria Manosas; Michelle M Spiering; Jean-François Allemand; Stephen J Benkovic; David Bensimon; Vincent Croquette
Single-molecule mechanical identification and sequencing.
Nature methods 2012;9(4):367-72.
2012: John W Tomsho; Stephen J Benkovic
Elucidation of the mechanism of the reaction between phenylboronic acid and a model diol, Alizarin Red S.
The Journal of organic chemistry 2012;77(5):2098-106.
2012: Vanja Stojković; Daniel Willmer; Stephen J Benkovic; Laura L Perissinotti; Amnon Kohen
Effects of the donor-acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction.
Journal of the American Chemical Society 2012;134(3):1738-45.
2011: Gordon G Hammes; Stephen J Benkovic; Sharon Hammes-Schiffer
Flexibility, diversity, and cooperativity: pillars of enzyme catalysis.
Biochemistry 2011;50(48):10422-30.
2011: Florence Verrier; Songon AN; Huayun Deng; Ye Fang; ANN FERRIE; Minjoung Kyoung; Haiyan Sun; Stephen J Benkovic
GPCRs regulate the assembly of a multienzyme complex for purine biosynthesis.
Nature chemical biology 2011;7(12):909-15.
2011: Stephen J Benkovic; Kevin D Raney
Mechanisms: molecular machines.
Current opinion in chemical biology 2011;15(5):577-9.
2011: Stephen J Baker; John W Tomsho; Stephen J Benkovic
Boron-containing inhibitors of synthetases.
Chemical Society reviews 2011;40(8):4279-85.
2011: Travis S Young; Douglas D Young; Insha Ahmad; Stephen J Benkovic; John M Louis; Peter G Schultz
Evolution of cyclic peptide protease inhibitors.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(27):11052-6.
2011: Gira Bhabha; Jeeyeon Lee; Damian C Ekiert; Jongsik Gam; Ian A Wilson; H Jane Dyson; Stephen J Benkovic; Peter E Wright
A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.
Science (New York, N.Y.) 2011;332(6026):234-8.
2011: Timothy John Herdendorf; Dustin W Albrecht; Stephen J Benkovic; Scott W Nelson
Biochemical characterization of bacteriophage T4 Mre11-Rad50 complex.
The Journal of biological chemistry 2011;286(4):2382-92.
2010: Vanja Stojković; Laura L Perissinotti; Jeeyeon Lee; Stephen J Benkovic; Amnon Kohen
The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer.
Chemical communications (Cambridge, England) 2010;46(47):8974-6.
2010: Yolanda Schaerli; Viktor Stein; Michelle M Spiering; Stephen J Benkovic; Chris Abell; Florian Hollfelder
Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification.
Nucleic acids research 2010;38(22):e201.
2010: Ravindra Kumar; Vishal C Nashine; Padmaja P Mishra; Stephen J Benkovic; Tae-Hee Lee
Stepwise loading of yeast clamp revealed by ensemble and single-molecule studies.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(46):19736-41.
2010: Vishal C Nashine; Sharon Hammes-Schiffer; Stephen J Benkovic
Coupled motions in enzyme catalysis.
Current opinion in chemical biology 2010;14(5):644-51.
2010: Scott W Nelson; Stephen J Benkovic
Response of the bacteriophage T4 replisome to noncoding lesions and regression of a stalled replication fork.
Journal of molecular biology 2010;401(5):743-56.
2010: Songon AN; Yijun Deng; John W Tomsho; Minjoung Kyoung; Stephen J Benkovic
Microtubule-assisted mechanism for functional metabolic macromolecular complex formation.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(29):12872-6.
2010: Senthil K Perumal; Kevin D Raney; Stephen J Benkovic
Analysis of the DNA translocation and unwinding activities of T4 phage helicases.
Methods (San Diego, Calif.) 2010;51(3):277-88.
2010: Senthil K Perumal; Hongjun Yue; Zhenxin Hu; Michelle M Spiering; Stephen J Benkovic
Single-molecule studies of DNA replisome function.
Biochimica et biophysica acta 2010;1804(5):1094-112.
2010: Songon AN; Minjoung Kyoung; Jasmina J Allen; Kevan M Shokat; Stephen J Benkovic
Dynamic regulation of a metabolic multi-enzyme complex by protein kinase CK2.
The Journal of biological chemistry 2010;285(15):11093-9.
2010: Jeeyeon Lee; Neela H Yennawar; Jongsik Gam; Stephen J Benkovic
Kinetic and structural characterization of dihydrofolate reductase from Streptococcus pneumoniae.
Biochemistry 2010;49(1):195-206.
2010: Ulrich Krauss; Jeeyeon Lee; Stephen J Benkovic; Karl-Erich Jaeger
LOVely enzymes - towards engineering light-controllable biocatalysts.
Microbial biotechnology 2010;3(1):15-23.
2010: Maria Manosas; Adrien Meglio; Michelle M Spiering; Fangyuan Ding; Stephen J Benkovic; François-Xavier Barre; Omar A Saleh; Jean-François Allemand; David Bensimon; Vincent Croquette
Magnetic tweezers for the study of DNA tracking motors.
Methods in enzymology 2010;475():297-320.
2009: Maria Manosas; Michelle M Spiering; Zhihao Zhuang; Stephen J Benkovic; Vincent Croquette
Coupling DNA unwinding activity with primer synthesis in the bacteriophage T4 primosome.
Nature chemical biology 2009;5(12):904-12.
2009: Sri Ranjini Arumugam; Tae-Hee Lee; Stephen J Benkovic
Investigation of stoichiometry of T4 bacteriophage helicase loader protein (gp59).
The Journal of biological chemistry 2009;284(43):29283-9.
2009: Hui Li; Walter Fast; Stephen J Benkovic
Structural and functional modularity of proteins in the de novo purine biosynthetic pathway.
Protein science : a publication of the Protein Society 2009;18(5):881-92.
2009: Scott W Nelson; Senthil K Perumal; Stephen J Benkovic
Processive and unidirectional translocation of monomeric UvsW helicase on single-stranded DNA.
Biochemistry 2009;48(5):1036-46.
2008: Michelle M Spiering; Scott W Nelson; Stephen J Benkovic
Repetitive lagging strand DNA synthesis by the bacteriophage T4 replisome.
Molecular bioSystems 2008;4(11):1070-4.
2008: Jeeyeon Lee; Madhusudan Natarajan; Vishal C Nashine; Michael Socolich; Tina Vo; William P Russ; Stephen J Benkovic; Rama Ranganathan
Surface sites for engineering allosteric control in proteins.
Science (New York, N.Y.) 2008;322(5900):438-42.
2008: Scott W Nelson; Ravindra Kumar; Stephen J Benkovic
RNA primer handoff in bacteriophage T4 DNA replication: the role of single-stranded DNA-binding protein and polymerase accessory proteins.
The Journal of biological chemistry 2008;283(33):22838-46.
2008: Nina M Goodey; Stephen J Benkovic
Allosteric regulation and catalysis emerge via a common route.
Nature chemical biology 2008;4(8):474-82.
2008: Zhihao Zhuang; Robert E Johnson; Lajos Haracska; Louise Prakash; Satya Prakash; Stephen J Benkovic
Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(14):5361-6.
2008: Songon AN; Ravindra Kumar; Erin Sheets; Stephen J Benkovic
Reversible compartmentalization of de novo purine biosynthetic complexes in living cells.
Science (New York, N.Y.) 2008;320(5872):103-6.
2008: Stephen J Benkovic; Gordon G Hammes; Sharon Hammes-Schiffer
Free-energy landscape of enzyme catalysis.
Biochemistry 2008;47(11):3317-21.
2008: John W Tomsho; Stephen J Benkovic
Unnatural translation initiation.
ACS chemical biology 2008;3(2):87-8.
2008: Todd A Naumann; Ali Tavassoli; Stephen J Benkovic
Genetic selection of cyclic peptide Dam methyltransferase inhibitors.
Chembiochem : a European journal of chemical biology 2008;9(2):194-7.
2007: Timothée Lionnet; Michelle M Spiering; Stephen J Benkovic; David Bensimon; Vincent Croquette
Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanism.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(50):19790-5.
2007: Lin Cheng; Todd A Naumann; Alexander R Horswill; Sue-Jean Hong; Bryan J Venters; John W Tomsho; Stephen J Benkovic; Kenneth Keiler
Discovery of antibacterial cyclic peptides that inhibit the ClpXP protease.
Protein science : a publication of the Protein Society 2007;16(8):1535-42.
2007: Fernando L Rock; Weimin Mao; Anna Yaremchuk; Michael Tukalo; Thibaut Crépin; Huchen Zhou; Yong-Kang Zhang; Vincent Hernandez; Tsutomu Akama; Stephen J Baker; Jacob J Plattner; Lucy Shapiro; Susan A Martinis; Stephen J Benkovic; Stephen Cusack; M R K Alley
An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site.
Science (New York, N.Y.) 2007;316(5832):1759-61.
2007: Scott W Nelson; Stephen J Benkovic
The T4 phage UvsW protein contains both DNA unwinding and strand annealing activities.
The Journal of biological chemistry 2007;282(1):407-16.
2007: Ali Tavassoli; Stephen J Benkovic
Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli.
Nature protocols 2007;2(5):1126-33.
2006: Alexander R Horswill; Stephen J Benkovic
Identifying small-molecule modulators of protein-protein interactions.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] 2006;Chapter 19():Unit 19.15.
2006: Stephen J Baker; Tsutomu Akama; Yong-Kang Zhang; Vittorio Sauro; Chetan Pandit; Rajeshwar Singh; Maureen Kully; Jehangir Khan; Jacob J Plattner; Stephen J Benkovic; Ving Lee; Kirk R Maples
Identification of a novel boron-containing antibacterial agent (AN0128) with anti-inflammatory activity, for the potential treatment of cutaneous diseases.
Bioorganic & medicinal chemistry letters 2006;16(23):5963-7.
2006: Lin Wang; Nina M Goodey; Stephen J Benkovic; Amnon Kohen
Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(43):15753-8.
2006: Lin Wang; Nina M Goodey; Stephen J Benkovic; Amnon Kohen
The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2006;361(1472):1307-15.
2006: R Derike Smiley; Zhihao Zhuang; Stephen J Benkovic; Gordon G Hammes
Single-molecule investigation of the T4 bacteriophage DNA polymerase holoenzyme: multiple pathways of holoenzyme formation.
Biochemistry 2006;45(26):7990-7.
2006: Zhihao Zhuang; Anthony J Berdis; Stephen J Benkovic
An alternative clamp loading pathway via the T4 clamp loader gp44/62-DNA complex.
Biochemistry 2006;45(26):7976-89.
2006: Manish C Saraf; Gregory L Moore; Nina M Goodey; Vania Y Cao; Stephen J Benkovic; Costas D Maranas
IPRO: an iterative computational protein library redesign and optimization procedure.
Biophysical journal 2006;90(11):4167-80.
2006: Stephen J Benkovic; Sharon Hammes-Schiffer
Biochemistry. Enzyme motions inside and out.
Science (New York, N.Y.) 2006;312(5771):208-9.
2006: Scott W Nelson; Jingsong Yang; Stephen J Benkovic
Site-directed mutations of T4 helicase loading protein (gp59) reveal multiple modes of DNA polymerase inhibition and the mechanism of unlocking by gp41 helicase.
The Journal of biological chemistry 2006;281(13):8697-706.
2006: Zhihao Zhuang; Bonita L Yoder; Peter M J Burgers; Stephen J Benkovic
The structure of a ring-opened proliferating cell nuclear antigen-replication factor C complex revealed by fluorescence energy transfer.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(8):2546-51.
2006: Lin Wang; Scott Tharp; Tzvia Selzer; Stephen J Benkovic; Amnon Kohen
Effects of a distal mutation on active site chemistry.
Biochemistry 2006;45(5):1383-92.
2006: Hee-Sung Park; Sung-Hun Nam; Jin Kak Lee; Chang No Yoon; Bengt Mannervik; Stephen J Benkovic; Hak-Sung Kim
Design and evolution of new catalytic activity with an existing protein scaffold.
Science (New York, N.Y.) 2006;311(5760):535-8.
2006: Jingsong Yang; Scott W Nelson; Stephen J Benkovic
The control mechanism for lagging strand polymerase recycling during bacteriophage T4 DNA replication.
Molecular cell 2006;21(2):153-64.
2006: Sharon Hammes-Schiffer; Stephen J Benkovic
Relating protein motion to catalysis.
Annual review of biochemistry 2006;75():519-41.
2005: Todd A Naumann; Sergey N Savinov; Stephen J Benkovic
Engineering an affinity tag for genetically encoded cyclic peptides.
Biotechnology and bioengineering 2005;92(7):820-30.
2005: Nina M Antikainen; R Derike Smiley; Stephen J Benkovic; Gordon G Hammes
Conformation coupled enzyme catalysis: single-molecule and transient kinetics investigation of dihydrofolate reductase.
Biochemistry 2005;44(51):16835-43.
2005: Stephen J Benkovic; Stephen J Baker; M R K Alley; Youn-Hi Woo; Yong-Kang Zhang; Tsutomu Akama; Weimin Mao; Justin Baboval; P T Ravi Rajagopalan; Mark Wall; Lyn Sue Kahng; Ali Tavassoli; Lucy Shapiro
Identification of borinic esters as inhibitors of bacterial cell growth and bacterial methyltransferases, CcrM and MenH.
Journal of medicinal chemistry 2005;48(23):7468-76.
2005: Karl E Griswold; Yasuaki Kawarasaki; Nada Ghoneim; Stephen J Benkovic; Brent L Iverson; george Georgiou
Evolution of highly active enzymes by homology-independent recombination.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(29):10082-7.
2005: Jingsong Yang; Jun Xi; Zhihao Zhuang; Stephen J Benkovic
The oligomeric T4 primase is the functional form during replication.
The Journal of biological chemistry 2005;280(27):25416-23.
2005: Jun Xi; Zhiquan Zhang; Zhihao Zhuang; Jingsong Yang; Michelle M Spiering; Gordon G Hammes; Stephen J Benkovic
Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome.
Biochemistry 2005;44(21):7747-56.
2005: Heng Cheng; Inhwan Hwang; Youhoon Chong; Ali Tavassoli; Michael E Webb; Yan Zhang; Ian A Wilson; Stephen J Benkovic; Dale L Boger
Synthesis and biological evaluation of N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid as a potential inhibitor of GAR Tfase and the de novo purine biosynthetic pathway.
Bioorganic & medicinal chemistry 2005;13(10):3593-9.
2005: Youhoon Chong; Inhwan Hwang; Ali Tavassoli; Yan Zhang; Ian A Wilson; Stephen J Benkovic; Dale L Boger
Synthesis and biological evaluation of alpha- and gamma-carboxamide derivatives of 10-CF3CO-DDACTHF.
Bioorganic & medicinal chemistry 2005;13(10):3587-92.
2005: Heng Cheng; Youhoon Chong; Inhwan Hwang; Ali Tavassoli; Yan Zhang; Ian A Wilson; Stephen J Benkovic; Dale L Boger
Design, synthesis, and biological evaluation of 10-methanesulfonyl-DDACTHF, 10-methanesulfonyl-5-DACTHF, and 10-methylthio-DDACTHF as potent inhibitors of GAR Tfase and the de novo purine biosynthetic pathway.
Bioorganic & medicinal chemistry 2005;13(10):3577-85.
2005: Youn-Hi Woo; P T Ravi Rajagopalan; Stephen J Benkovic
A nonradioactive DNA methyltransferase assay adaptable to high-throughput screening.
Analytical biochemistry 2005;340(2):336-40.
2005: Kim F Wong; Tzvia Selzer; Stephen J Benkovic; Sharon Hammes-Schiffer
Impact of distal mutations on the network of coupled motions correlated to hydride transfer in dihydrofolate reductase.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(19):6807-12.
2005: Ali Tavassoli; Stephen J Benkovic
Genetically selected cyclic-peptide inhibitors of AICAR transformylase homodimerization.
Angewandte Chemie (International ed. in English) 2005;44(18):2760-3.
2005: Alexander R Horswill; Stephen J Benkovic
Cyclic peptides, a chemical genetics tool for biologists.
Cell cycle (Georgetown, Tex.) 2005;4(4):552-5.
2005: Mona T Norcum; J Anthony Warrington; Michelle M Spiering; Faoud T Ishmael; Michael Trakselis; Stephen J Benkovic
Architecture of the bacteriophage T4 primosome: electron microscopy studies of helicase (gp41) and primase (gp61).
Proceedings of the National Academy of Sciences of the United States of America 2005;102(10):3623-6.
2005: Zhiquan Zhang; Michelle M Spiering; Michael Trakselis; Faoud T Ishmael; Jun Xi; Stephen J Benkovic; Gordon G Hammes
Assembly of the bacteriophage T4 primosome: single-molecule and ensemble studies.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(9):3254-9.
2005: Jun Xi; Zhihao Zhuang; Zhiquan Zhang; Tzvia Selzer; Michelle M Spiering; Gordon G Hammes; Stephen J Benkovic
Interaction between the T4 helicase-loading protein (gp59) and the DNA polymerase (gp43): a locking mechanism to delay replication during replisome assembly.
Biochemistry 2005;44(7):2305-18.
2004: Rani P Venkitakrishnan; Eduardo Zaborowski; Dan McElheny; Stephen J Benkovic; H Jane Dyson; Peter Wright
Conformational changes in the active site loops of dihydrofolate reductase during the catalytic cycle.
Biochemistry 2004;43(51):16046-55.
2004: Catherine M Joyce; Stephen J Benkovic
DNA polymerase fidelity: kinetics, structure, and checkpoints.
Biochemistry 2004;43(45):14317-24.
2004: Alexander R Horswill; Sergey N Savinov; Stephen J Benkovic
A systematic method for identifying small-molecule modulators of protein-protein interactions.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(44):15591-6.
2004: David Millar; Michael Trakselis; Stephen J Benkovic
On the solution structure of the T4 sliding clamp (gp45).
Biochemistry 2004;43(40):12723-7.
2004: Zhihao Zhuang; Michelle M Spiering; Anthony J Berdis; Michael Trakselis; Stephen J Benkovic
'Screw-cap' clamp loader proteins that thread.
Nature structural & molecular biology 2004;11(7):580-1.
2004: Jingsong Yang; Zhihao Zhuang; Rosa Maria Roccasecca; Michael Trakselis; Stephen J Benkovic
The dynamic processivity of the T4 DNA polymerase during replication.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(22):8289-94.
2004: R Steven Sikorski; Lin Wang; Kelli A Markham; P T Ravi Rajagopalan; Stephen J Benkovic; Amnon Kohen
Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis.
Journal of the American Chemical Society 2004;126(15):4778-9.
2004: Manish C Saraf; Alexander R Horswill; Stephen J Benkovic; Costas D Maranas
FamClash: a method for ranking the activity of engineered enzymes.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(12):4142-7.
2004: Zhiquan Zhang; P T Ravi Rajagopalan; Tzvia Selzer; Stephen J Benkovic; Gordon G Hammes
Single-molecule and transient kinetics investigation of the interaction of dihydrofolate reductase with NADPH and dihydrofolate.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(9):2764-9.
2004: Alexander R Horswill; Todd A Naumann; Stephen J Benkovic
Using incremental truncation to create libraries of hybrid enzymes.
Methods in enzymology 2004;388():50-60.
2003: Michael Trakselis; Rosa Maria Roccasecca; Jingsong Yang; Ann M Valentine; Stephen J Benkovic
Dissociative properties of the proteins within the bacteriophage T4 replisome.
The Journal of biological chemistry 2003;278(50):49839-49.
2003: Jingsong Yang; Michael Trakselis; Rosa Maria Roccasecca; Stephen J Benkovic
The application of a minicircle substrate in the study of the coordinated T4 DNA replication.
The Journal of biological chemistry 2003;278(50):49828-38.
2003: Yasuaki Kawarasaki; Karl E Griswold; James D Stevenson; Tzvia Selzer; Stephen J Benkovic; Brent L Iverson; george Georgiou
Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.
Nucleic acids research 2003;31(21):e126.
2003: Seung-Goo Lee; Stefan Lutz; Stephen J Benkovic
On the structural and functional modularity of glycinamide ribonucleotide formyltransferases.
Protein science : a publication of the Protein Society 2003;12(10):2206-14.
2003: Joel Desharnais; Inhwan Hwang; Yan Zhang; Ali Tavassoli; Justin Baboval; Stephen J Benkovic; Ian A Wilson; Dale L Boger
Design, synthesis and biological evaluation of 10-CF3CO-DDACTHF analogues and derivatives as inhibitors of GAR Tfase and the de novo purine biosynthetic pathway.
Bioorganic & medicinal chemistry 2003;11(20):4511-21.
2003: Thomas H Marsilje; Michael P Hedrick; Joel Desharnais; Kevin Capps; Ali Tavassoli; Yan Zhang; Ian A Wilson; Stephen J Benkovic; Dale L Boger
10-(2-benzoxazolcarbonyl)-5,10-dideaza-acyclic-5,6,7,8-tetrahydrofolic acid: a potential inhibitor of GAR transformylase and AICAR transformylase.
Bioorganic & medicinal chemistry 2003;11(20):4503-9.
2003: Thomas H Marsilje; Michael P Hedrick; Joel Desharnais; Ali Tavassoli; Yan Zhang; Ian A Wilson; Stephen J Benkovic; Dale L Boger
Design, synthesis, and biological evaluation of simplified alpha-keto heterocycle, trifluoromethyl ketone, and formyl substituted folate analogues as potential inhibitors of GAR transformylase and AICAR transformylase.
Bioorganic & medicinal chemistry 2003;11(20):4487-501.
2003: Dennis W Wolan; Samantha E Greasley; Mark J Wall; Stephen J Benkovic; Ian A Wilson
Structure of avian AICAR transformylase with a multisubstrate adduct inhibitor beta-DADF identifies the folate binding site.
Biochemistry 2003;42(37):10904-14.
2003: Stephen J Benkovic; Sharon Hammes-Schiffer
A perspective on enzyme catalysis.
Science (New York, N.Y.) 2003;301(5637):1196-202.
2003: Pamela Pineda; Aaron Kanter; R Scott McIvor; Stephen J Benkovic; Andre Rosowsky; Carston R Wagner
Dihydrofolate reductase mutant with exceptional resistance to methotrexate but not to trimetrexate.
Journal of medicinal chemistry 2003;46(14):2816-8.
2003: James J A Huntley; Walter Fast; Stephen J Benkovic; Peter Wright; H Jane Dyson
Role of a solvent-exposed tryptophan in the recognition and binding of antibiotic substrates for a metallo-beta-lactamase.
Protein science : a publication of the Protein Society 2003;12(7):1368-75.
2003: Yan Zhang; Joel Desharnais; Thomas H Marsilje; Chenglong Li; Michael P Hedrick; Lata T Gooljarsingh; Ali Tavassoli; Stephen J Benkovic; Arthur J Olson; Dale L Boger; Ian A Wilson
Rational design, synthesis, evaluation, and crystal structure of a potent inhibitor of human GAR Tfase: 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid.
Biochemistry 2003;42(20):6043-56.
2003: Sergey N Savinov; Ralph Hirschmann; Stephen J Benkovic; Amos B Smith
Investigation of an antibody-ligase. Evidence for strain-induced catalysis.
Bioorganic & medicinal chemistry letters 2003;13(7):1321-4.
2003: Michael Trakselis; Anthony J Berdis; Stephen J Benkovic
Examination of the role of the clamp-loader and ATP hydrolysis in the formation of the bacteriophage T4 polymerase holoenzyme.
Journal of molecular biology 2003;326(2):435-51.
2003: Faoud T Ishmael; Michael Trakselis; Stephen J Benkovic
Protein-protein interactions in the bacteriophage T4 replisome. The leading strand holoenzyme is physically linked to the lagging strand holoenzyme and the primosome.
The Journal of biological chemistry 2003;278(5):3145-52.
2003: Steven M Firestine; Frank Salinas; Stephen J Benkovic
Using transcriptional-based systems for in vivo enzyme screening.
Methods in molecular biology (Clifton, N.J.) 2003;205():315-27.
2003: Ernesto Abel-Santos; Charles Scott; Stephen J Benkovic
Use of inteins for the in vivo production of stable cyclic peptide libraries in E. coli.
Methods in molecular biology (Clifton, N.J.) 2003;205():281-94.
2002: Stefan Lutz; Walter Fast; Stephen J Benkovic
A universal, vector-based system for nucleic acid reading-frame selection.
Protein engineering 2002;15(12):1025-30.
2002: P T Ravi Rajagopalan; Stefan Lutz; Stephen J Benkovic
Coupling interactions of distal residues enhance dihydrofolate reductase catalysis: mutational effects on hydride transfer rates.
Biochemistry 2002;41(42):12618-28.
2002: P T Ravi Rajagopalan; Zhiquan Zhang; Lynn McCourt; Mary Dwyer; Stephen J Benkovic; Gordon G Hammes
Interaction of dihydrofolate reductase with methotrexate: ensemble and single-molecule kinetics.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(21):13481-6.
2002: Thomas H Marsilje; Marc A Labroli; Michael P Hedrick; Qing Jin; Joel Desharnais; Stephen J Baker; Lata T Gooljarsingh; Joseph Ramcharan; Ali Tavassoli; Yan Zhang; Ian A Wilson; G Peter Beardsley; Stephen J Benkovic; Dale L Boger
10-Formyl-5,10-dideaza-acyclic-5,6,7,8-tetrahydrofolic acid (10-formyl-DDACTHF): a potent cytotoxic agent acting by selective inhibition of human GAR Tfase and the de novo purine biosynthetic pathway.
Bioorganic & medicinal chemistry 2002;10(8):2739-49.
2002: James B Thoden; Steven M Firestine; Stephen J Benkovic; Hazel M Holden
PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site.
The Journal of biological chemistry 2002;277(26):23898-908.
2002: Faoud T Ishmael; Stephen C Alley; Stephen J Benkovic
Assembly of the bacteriophage T4 helicase: architecture and stoichiometry of the gp41-gp59 complex.
The Journal of biological chemistry 2002;277(23):20555-62.
2002: Pratul K Agarwal; Salomon R Billeter; P T Ravi Rajagopalan; Stephen J Benkovic; Sharon Hammes-Schiffer
Network of coupled promoting motions in enzyme catalysis.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(5):2794-9.
2002: P T Ravi Rajagopalan; Stephen J Benkovic
Preorganization and protein dynamics in enzyme catalysis.
Chemical record (New York, N.Y.) 2002;2(1):24-36.
2001: Ann M Valentine; Faoud T Ishmael; Vincent K Shier; Stephen J Benkovic
A zinc ribbon protein in DNA replication: primer synthesis and macromolecular interactions by the bacteriophage T4 primase.
Biochemistry 2001;40(50):15074-85.
2001: Samantha E Greasley; Thomas H Marsilje; H Cai; S Baker; Stephen J Benkovic; Dale L Boger; Ian A Wilson
Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase.
Biochemistry 2001;40(45):13538-47.
2001: Michael Trakselis; Stephen J Benkovic
Intricacies in ATP-dependent clamp loading: variations across replication systems.
Structure (London, England : 1993) 2001;9(11):999-1004.
2001: Stephen C Alley; Michael Trakselis; Uljana Mayer; Faoud T Ishmael; A Daniel Jones; Stephen J Benkovic
Building a replisome solution structure by elucidation of protein-protein interactions in the bacteriophage T4 DNA polymerase holoenzyme.
The Journal of biological chemistry 2001;276(42):39340-9.
2001: Stefan Lutz; Marc Ostermeier; Gregory L Moore; Costas D Maranas; Stephen J Benkovic
Creating multiple-crossover DNA libraries independent of sequence identity.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(20):11248-53.
2001: Michael Trakselis; Uljana Mayer; Faoud T Ishmael; Rosa Maria Roccasecca; Stephen J Benkovic
Dynamic protein interactions in the bacteriophage T4 replisome.
Trends in biochemical sciences 2001;26(9):566-72.
2001: Michael J Osborne; Jason R Schnell; Stephen J Benkovic; H Jane Dyson; Peter Wright
Backbone dynamics in dihydrofolate reductase complexes: role of loop flexibility in the catalytic mechanism.
Biochemistry 2001;40(33):9846-59.
2001: Charles Scott; Ernesto Abel-Santos; A D Jones; Stephen J Benkovic
Structural requirements for the biosynthesis of backbone cyclic peptide libraries.
Chemistry & biology 2001;8(8):801-15.
2001: Michael Trakselis; Stephen C Alley; Ernesto Abel-Santos; Stephen J Benkovic
Creating a dynamic picture of the sliding clamp during T4 DNA polymerase holoenzyme assembly by using fluorescence resonance energy transfer.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8368-75.
2001: Faoud T Ishmael; Stephen C Alley; Stephen J Benkovic
Identification and mapping of protein-protein interactions between gp32 and gp59 by cross-linking.
The Journal of biological chemistry 2001;276(27):25236-42.
2001: Faoud T Ishmael; Mona T Norcum; Stephen J Benkovic; Judith S Bond
Multimeric structure of the secreted meprin A metalloproteinase and characterization of the functional protomer.
The Journal of biological chemistry 2001;276(25):23207-11.
2001: Lata T Gooljarsingh; Joseph Ramcharan; Simon Gilroy; Stephen J Benkovic
Localization of GAR transformylase in Escherichia coli and mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(12):6565-70.
2001: JH Shim; Mark Wall; Stephen J Benkovic; Natalia Díaz; Dimas Suárez; Kenneth M Merz
Evaluation of the catalytic mechanism of AICAR transformylase by pH-dependent kinetics, mutagenesis, and quantum chemical calculations.
Journal of the American Chemical Society 2001;123(20):4687-96.
2001: Vincent K Shier; C J Hancey; Stephen J Benkovic
Identification of the active oligomeric state of an essential adenine DNA methyltransferase from Caulobacter crescentus.
The Journal of biological chemistry 2001;276(18):14744-51.
2001: Samantha E Greasley; P Horton; Joseph Ramcharan; G Peter Beardsley; Stephen J Benkovic; Ian A Wilson
Crystal structure of a bifunctional transformylase and cyclohydrolase enzyme in purine biosynthesis.
Nature structural biology 2001;8(5):402-6.
2001: Gregory L Moore; Costas D Maranas; Stefan Lutz; Stephen J Benkovic
Predicting crossover generation in DNA shuffling.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(6):3226-31.
2001: Stefan Lutz; Marc Ostermeier; Stephen J Benkovic
Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.
Nucleic acids research 2001;29(4):E16.
2001: Walter Fast; Z Wang; Stephen J Benkovic
Familial mutations and zinc stoichiometry determine the rate-limiting step of nitrocefin hydrolysis by metallo-beta-lactamase from Bacteroides fragilis.
Biochemistry 2001;40(6):1640-50.
2001: DC Wahnon; Vincent K Shier; Stephen J Benkovic
Mechanism-based inhibition of an essential bacterial adenine DNA methyltransferase: rationally designed antibiotics.
Journal of the American Chemical Society 2001;123(5):976-7.
2001: GP Miller; DC Wahnon; Stephen J Benkovic
Interloop contacts modulate ligand cycling during catalysis by Escherichia coli dihydrofolate reductase.
Biochemistry 2001;40(4):867-75.
2001: Stephen J Benkovic; Ann M Valentine; Frank Salinas
Replisome-mediated DNA replication.
Annual review of biochemistry 2001;70():181-208.
2000: Mark Wall; JH Shim; Stephen J Benkovic
Human AICAR transformylase: role of the 4-carboxamide of AICAR in binding and catalysis.
Biochemistry 2000;39(37):11303-11.
2000: Stefan Lutz; Stephen J Benkovic
Homology-independent protein engineering.
Current opinion in biotechnology 2000;11(4):319-24.
2000: James B Thoden; Steven M Firestine; A Nixon; Stephen J Benkovic; Hazel M Holden
Molecular structure of Escherichia coli PurT-encoded glycinamide ribonucleotide transformylase.
Biochemistry 2000;39(30):8791-802.
2000: Dale L Boger; Thomas H Marsilje; R A Castro; Michael P Hedrick; Qing Jin; Stephen J Baker; JH Shim; Stephen J Benkovic
Design, synthesis, and biological evaluation of fluoronitrophenyl substituted folate analogues as potential inhibitors of GAR transformylase and AICAR transformylase.
Bioorganic & medicinal chemistry letters 2000;10(13):1471-5.
2000: Frank Salinas; Stephen J Benkovic
Characterization of bacteriophage T4-coordinated leading- and lagging-strand synthesis on a minicircle substrate.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(13):7196-201.
2000: Thomas C Bruice; Stephen J Benkovic
Chemical basis for enzyme catalysis.
Biochemistry 2000;39(21):6267-74.
2000: Dale L Boger; Marc A Labroli; Thomas H Marsilje; Qing Jin; Michael P Hedrick; Stephen J Baker; JH Shim; Stephen J Benkovic
Conformationally restricted analogues designed for selective inhibition of GAR Tfase versus thymidylate synthase or dihydrofolate reductase.
Bioorganic & medicinal chemistry 2000;8(5):1075-86.
2000: AE Nixon; Stephen J Benkovic
Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme.
Protein engineering 2000;13(5):323-7.
2000: Steven M Firestine; Frank Salinas; AE Nixon; S J Baker; Stephen J Benkovic
Using an AraC-based three-hybrid system to detect biocatalysts in vivo.
Nature biotechnology 2000;18(5):544-7.
2000: Stephen C Alley; Ernesto Abel-Santos; Stephen J Benkovic
Tracking sliding clamp opening and closing during bacteriophage T4 DNA polymerase holoenzyme assembly.
Biochemistry 2000;39(11):3076-90.
2000: D B Smithrud; P A Benkovic; Stephen J Benkovic; V Roberts; J Liu; I Neagu; S Iwama; B W Phillips; A B Smith; Ralph Hirschmann
Cyclic peptide formation catalyzed by an antibody ligase.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(5):1953-8.
1999: Samantha E Greasley; M M Yamashita; H Cai; Stephen J Benkovic; Dale L Boger; Ian A Wilson
New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid.
Biochemistry 1999;38(51):16783-93.
1999: Marc Ostermeier; JH Shim; Stephen J Benkovic
A combinatorial approach to hybrid enzymes independent of DNA homology.
Nature biotechnology 1999;17(12):1205-9.
1999: Charles Scott; Ernesto Abel-Santos; M Wall; DC Wahnon; Stephen J Benkovic
Production of cyclic peptides and proteins in vivo.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(24):13638-43.
1999: SD Scrofani; John Chung; James J A Huntley; Stephen J Benkovic; Peter Wright; H Jane Dyson
NMR characterization of the metallo-beta-lactamase from Bacteroides fragilis and its interaction with a tight-binding inhibitor: role of an active-site loop.
Biochemistry 1999;38(44):14507-14.
1999: Marc Ostermeier; AE Nixon; Stephen J Benkovic
Incremental truncation as a strategy in the engineering of novel biocatalysts.
Bioorganic & medicinal chemistry 1999;7(10):2139-44.
1999: Z Wang; Walter Fast; Ann M Valentine; Stephen J Benkovic
Metallo-beta-lactamase: structure and mechanism.
Current opinion in chemical biology 1999;3(5):614-22.
1999: Mark Wall; JH Shim; Stephen J Benkovic
A multisubstrate adduct inhibitor of AICAR transformylase.
Journal of medicinal chemistry 1999;42(18):3421-4.
1999: Stephen C Alley; A Daniel Jones; P Soumillion; Stephen J Benkovic
The carboxyl terminus of the bacteriophage T4 DNA polymerase contacts its sliding clamp at the subunit interface.
The Journal of biological chemistry 1999;274(35):24485-9.
1999: Maria M Thayer; E H Olender; Andrew S Arvai; C K Koike; IL Canestrelli; J D Stewart; Stephen J Benkovic; Elizabeth D Getzoff; Victoria A Roberts
Structural basis for amide hydrolysis catalyzed by the 43C9 antibody.
Journal of molecular biology 1999;291(2):329-45.
1999: JH Shim; Stephen J Benkovic
Catalytic mechanism of Escherichia coli glycinamide ribonucleotide transformylase probed by site-directed mutagenesis and pH-dependent studies.
Biochemistry 1999;38(31):10024-31.
1999: Z Wang; Walter Fast; Stephen J Benkovic
On the mechanism of the metallo-beta-lactamase from Bacteroides fragilis.
Biochemistry 1999;38(31):10013-23.
1999: Stephen C Alley; Vincent K Shier; Ernesto Abel-Santos; Daniel Sexton; P Soumillion; Stephen J Benkovic
Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution.
Biochemistry 1999;38(24):7696-709.
1999: AE Nixon; Steven M Firestine; Frank Salinas; Stephen J Benkovic
Rational design of a scytalone dehydratase-like enzyme using a structurally homologous protein scaffold.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(7):3568-71.
1999: Marc Ostermeier; AE Nixon; JH Shim; Stephen J Benkovic
Combinatorial protein engineering by incremental truncation.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(7):3562-7.
1998: P Wu; N Nossal; Stephen J Benkovic
Kinetic characterization of a bacteriophage T4 antimutator DNA polymerase.
Biochemistry 1998;37(42):14748-55.
1998: W R Cannon; Stephen J Benkovic
Solvation, reorganization energy, and biological catalysis.
The Journal of biological chemistry 1998;273(41):26257-60.
1998: Anthony J Berdis; Stephen J Benkovic
Simultaneous formation of functional leading and lagging strand holoenzyme complexes on a small, defined DNA substrate.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(19):11128-33.
1998: Z Wang; Stephen J Benkovic
Purification, characterization, and kinetic studies of a soluble Bacteroides fragilis metallo-beta-lactamase that provides multiple antibiotic resistance.
The Journal of biological chemistry 1998;273(35):22402-8.
1998: Y Su; M M Yamashita; Samantha E Greasley; Craig A Mullen; JH Shim; Patricia Jennings; Stephen J Benkovic; Ian A Wilson
A pH-dependent stabilization of an active site loop observed from low and high pH crystal structures of mutant monomeric glycinamide ribonucleotide transformylase at 1.8 to 1.9 A.
Journal of molecular biology 1998;281(3):485-99.
1998: JH Shim; Stephen J Benkovic
Evaluation of the kinetic mechanism of Escherichia coli glycinamide ribonucleotide transformylase.
Biochemistry 1998;37(24):8776-82.
1998: Dale L Boger; M J Kochanny; H Cai; D Wyatt; Paul Kitos; MS Warren; Joseph Ramcharan; Lata T Gooljarsingh; Stephen J Benkovic
Design, synthesis, and evaluation of potential GAR and AICAR transformylase inhibitors.
Bioorganic & medicinal chemistry 1998;6(6):643-59.
1998: AE Nixon; Marc Ostermeier; Stephen J Benkovic
Hybrid enzymes: manipulating enzyme design.
Trends in biotechnology 1998;16(6):258-64.
1998: Daniel Sexton; B F Kaboord; Anthony J Berdis; Theodore E Carver; Stephen J Benkovic
Dissecting the order of bacteriophage T4 DNA polymerase holoenzyme assembly.
Biochemistry 1998;37(21):7749-56.
1998: GP Miller; Stephen J Benkovic
Strength of an interloop hydrogen bond determines the kinetic pathway in catalysis by Escherichia coli dihydrofolate reductase.
Biochemistry 1998;37(18):6336-42.
1998: GP Miller; Stephen J Benkovic
Deletion of a highly motional residue affects formation of the Michaelis complex for Escherichia coli dihydrofolate reductase.
Biochemistry 1998;37(18):6327-35.
1998: GP Miller; Stephen J Benkovic
Stretching exercises--flexibility in dihydrofolate reductase catalysis.
Chemistry & biology 1998;5(5):R105-13.
1998: Anthony J Berdis; I Lee; J K Coward; C Stephens; R Wright; Lucy Shapiro; Stephen J Benkovic
A cell cycle-regulated adenine DNA methyltransferase from Caulobacter crescentus processively methylates GANTC sites on hemimethylated DNA.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(6):2874-9.
1998: P Soumillion; Daniel Sexton; Stephen J Benkovic
Clamp subunit dissociation dictates bacteriophage T4 DNA polymerase holoenzyme disassembly.
Biochemistry 1998;37(7):1819-27.
1998: W Zhong; Stephen J Benkovic
Development of an internally quenched fluorescent substrate for Escherichia coli leader peptidase.
Analytical biochemistry 1998;255(1):66-73.
1997: Craig E Cameron; Stephen J Benkovic
Evidence for a functional role of the dynamics of glycine-121 of Escherichia coli dihydrofolate reductase obtained from kinetic analysis of a site-directed mutant.
Biochemistry 1997;36(50):15792-800.
1997: Theodore E Carver; Daniel Sexton; Stephen J Benkovic
Dissociation of bacteriophage T4 DNA polymerase and its processivity clamp after completion of Okazaki fragment synthesis.
Biochemistry 1997;36(47):14409-17.
1997: Daniel Sexton; Anthony J Berdis; Stephen J Benkovic
Assembly and disassembly of DNA polymerase holoenzyme.
Current opinion in chemical biology 1997;1(3):316-22.
1997: Dale L Boger; NE Haynes; MS Warren; Joseph Ramcharan; Paul Kitos; Stephen J Benkovic
Multisubstrate analogue based on 5,8,10-trideazafolate.
Bioorganic & medicinal chemistry 1997;5(9):1853-7.
1997: Dale L Boger; NE Haynes; MS Warren; Joseph Ramcharan; AE Marolewski; Paul Kitos; Stephen J Benkovic
Abenzyl 10-formyl-trideazafolic acid (abenzyl 10-formyl-TDAF): an effective inhibitor of glycinamide ribonucleotide transformylase.
Bioorganic & medicinal chemistry 1997;5(9):1847-52.
1997: Dale L Boger; NE Haynes; MS Warren; Joseph Ramcharan; Paul Kitos; Stephen J Benkovic
Functionalized analogues of 5,8,10-trideazafolate: development of an enzyme-assembled tight binding inhibitor of GAR Tfase and a potential irreversible inhibitor of AICAR Tfase.
Bioorganic & medicinal chemistry 1997;5(9):1839-46.
1997: Dale L Boger; NE Haynes; MS Warren; Lata T Gooljarsingh; Joseph Ramcharan; Paul Kitos; Stephen J Benkovic
Functionalized analogues of 5,8,10-trideazafolate as potential inhibitors of GAR Tfase or AICAR Tfase.
Bioorganic & medicinal chemistry 1997;5(9):1831-8.
1997: Dale L Boger; NE Haynes; Paul Kitos; MS Warren; Joseph Ramcharan; AE Marolewski; Stephen J Benkovic
10-Formyl-5,8,10-trideazafolic acid (10-formyl-TDAF): a potent inhibitor of glycinamide ribonucleotide transformylase.
Bioorganic & medicinal chemistry 1997;5(9):1817-30.
1997: W R Cannon; Barbara J Garrison; Stephen J Benkovic
Consideration of the pH-dependent inhibition of dihydrofolate reductase by methotrexate.
Journal of molecular biology 1997;271(4):656-68.
1997: D B Smithrud; Stephen J Benkovic
The state of antibody catalysis.
Current opinion in biotechnology 1997;8(4):459-66.
1997: Craig E Cameron; M Ghosh; S F Le Grice; Stephen J Benkovic
Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(13):6700-5.
1997: AE Marolewski; K M Mattia; MS Warren; Stephen J Benkovic
Formyl phosphate: a proposed intermediate in the reaction catalyzed by Escherichia coli PurT GAR transformylase.
Biochemistry 1997;36(22):6709-16.
1997: Anthony J Berdis; Stephen J Benkovic
Mechanism of bacteriophage T4 DNA holoenzyme assembly: the 44/62 protein acts as a molecular motor.
Biochemistry 1997;36(10):2733-43.
1997: GP Miller; B A Posner; Stephen J Benkovic
Expanding the 43C9 class of catalytic antibodies using a chain-shuffling approach.
Bioorganic & medicinal chemistry 1997;5(3):581-90.
1997: AE Nixon; MS Warren; Stephen J Benkovic
Assembly of an active enzyme by the linkage of two protein modules.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(4):1069-73.
1997: MS Warren; Stephen J Benkovic
Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase.
Protein engineering 1997;10(1):63-8.
1996: Anthony J Berdis; P Soumillion; Stephen J Benkovic
The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme complex formation.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(23):12822-7.
1996: Daniel Sexton; Theodore E Carver; Anthony J Berdis; Stephen J Benkovic
Protein-protein and protein-DNA interactions at the bacteriophage T4 DNA replication fork. Characterization of a fluorescently labeled DNA polymerase sliding clamp.
The Journal of biological chemistry 1996;271(45):28045-51.
1996: Steven M Firestine; AE Nixon; Stephen J Benkovic
Threading your way to protein function.
Chemistry & biology 1996;3(10):779-83.
1996: M W Crowder; Z Wang; S L Franklin; E P Zovinka; Stephen J Benkovic
Characterization of the metal-binding sites of the beta-lactamase from Bacteroides fragilis.
Biochemistry 1996;35(37):12126-32.
1996: Anthony J Berdis; Stephen J Benkovic
Role of adenosine 5'-triphosphate hydrolysis in the assembly of the bacteriophage T4 DNA replication holoenzyme complex.
Biochemistry 1996;35(28):9253-65.
1996: MS Warren; A E Marolewski; Stephen J Benkovic
A rapid screen of active site mutants in glycinamide ribonucleotide transformylase.
Biochemistry 1996;35(27):8855-62.
1996: K D Raney; Theodore E Carver; Stephen J Benkovic
Stoichiometry and DNA unwinding by the bacteriophage T4 41:59 helicase.
The Journal of biological chemistry 1996;271(24):14074-81.
1996: M W Frey; S T Frey; W D Horrocks; B F Kaboord; Stephen J Benkovic
Elucidation of the metal-binding properties of the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase by lanthanide(III) luminescence spectroscopy.
Chemistry & biology 1996;3(5):393-403.
1996: B F Kaboord; Stephen J Benkovic
Dual role of the 44/62 protein as a matchmaker protein and DNA polymerase chaperone during assembly of the bacteriophage T4 holoenzyme complex.
Biochemistry 1996;35(3):1084-92.
1996: C L Brummel; J C Vickerman; Steve Carr; M E Hemling; G D Roberts; W Johnson; J Weinstock; D Gaitanopoulos; Stephen J Benkovic; Nicholas Winograd
Evaluation of mass spectrometric methods applicable to the direct analysis of non-peptide bead-bound combinatorial libraries.
Analytical chemistry 1996;68(2):237-42.
1995: C R Wagner; Z Huang; Scott Singleton; Stephen J Benkovic
Molecular basis for nonadditive mutational effects in Escherichia coli dihydrofolate reductase.
Biochemistry 1995;34(48):15671-80.
1995: K D Raney; Stephen J Benkovic
Bacteriophage T4 Dda helicase translocates in a unidirectional fashion on single-stranded DNA.
The Journal of biological chemistry 1995;270(38):22236-42.
1995: D M Epstein; Stephen J Benkovic; Peter Wright
Dynamics of the dihydrofolate reductase-folate complex: catalytic sites and regions known to undergo conformational change exhibit diverse dynamical features.
Biochemistry 1995;34(35):11037-48.
1995: I Lee; J D Stewart; W Zhong; Stephen J Benkovic
A chemiluminescent immunoassay for the identification of functional single-chain antibodies.
Analytical biochemistry 1995;230(1):62-7.
1995: M W Frey; L C Sowers; David P Millar; Stephen J Benkovic
The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase.
Biochemistry 1995;34(28):9185-92.
1995: R T Carr; S Balasubramanian; P C Hawkins; Stephen J Benkovic
Mechanism of metal-independent hydroxylation by Chromobacterium violaceum phenylalanine hydroxylase.
Biochemistry 1995;34(22):7525-32.
1995: C Klein; P Chen; J H Arevalo; Enrico STURA; A Marolewski; MS Warren; Stephen J Benkovic; Ian A Wilson
Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution.
Journal of molecular biology 1995;249(1):153-75.
1995: B F Kaboord; Stephen J Benkovic
Accessory proteins function as matchmakers in the assembly of the T4 DNA polymerase holoenzyme.
Current biology : CB 1995;5(2):149-57.
1995: J F Krebs; Gary Siuzdak; H Jane Dyson; J D Stewart; Stephen J Benkovic
Detection of a catalytic antibody species acylated at the active site by electrospray mass spectrometry.
Biochemistry 1995;34(3):720-3.
1995: T L Capson; Stephen J Benkovic; N G Nossal
Photochemical cross-linking of DNA replication proteins at primer terminus.
Methods in enzymology 1995;262():449-56.
1995: Stephen J Benkovic; Craig E Cameron
Kinetic analysis of nucleotide incorporation and misincorporation by Klenow fragment of Escherichia coli DNA polymerase I.
Methods in enzymology 1995;262():257-69.
1995: J D Stewart; I Lee; B A Posner; Stephen J Benkovic
Expression of properly folded catalytic antibodies in Escherichia coli.
Methods in enzymology 1995;249():507-19.
1994: J A Peliska; S Balasubramanian; D P Giedroc; Stephen J Benkovic
Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity.
Biochemistry 1994;33(46):13817-23.
1994: Z Huang; C R Wagner; Stephen J Benkovic
Nonadditivity of mutational effects at the folate binding site of Escherichia coli dihydrofolate reductase.
Biochemistry 1994;33(38):11576-85.
1994: J A Smiley; Stephen J Benkovic
Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli: antibodies for orotate decarboxylation.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(18):8319-23.
1994: V Gopalakrishnan; Stephen J Benkovic
Spatial relationship between polymerase and exonuclease active sites of phage T4 DNA polymerase enzyme.
The Journal of biological chemistry 1994;269(33):21123-6.
1994: J D Stewart; J F Krebs; Gary Siuzdak; Anthony J Berdis; D B Smithrud; Stephen J Benkovic
Dissection of an antibody-catalyzed reaction.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(16):7404-9.
1994: S Balasubramanian; R T Carr; C J Bender; Jack Peisach; Stephen J Benkovic
Identification of metal ligands in Cu(II)-inhibited Chromobacterium violaceum phenylalanine hydroxylase by electron spin echo envelope modulation analysis of histidine to serine mutations.
Biochemistry 1994;33(28):8532-7.
1994: K D Raney; L C Sowers; David P Millar; Stephen J Benkovic
A fluorescence-based assay for monitoring helicase activity.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(14):6644-8.
1994: Christopher J Falzone; John Cavanagh; M Cowart; Arthur G Palmer; C Robert Matthews; Stephen J Benkovic; Peter Wright
1H, 15N and 13C resonance assignments, secondary structure, and the conformation of substrate in the binary folate complex of Escherichia coli dihydrofolate reductase.
Journal of biomolecular NMR 1994;4(3):349-66.
1994: C L Brummel; I N Lee; Y Zhou; Stephen J Benkovic; Nicholas Winograd
A mass spectrometric solution to the address problem of combinatorial libraries.
Science (New York, N.Y.) 1994;264(5157):399-402.
1994: J A Peliska; Stephen J Benkovic
Fidelity of in vitro DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
Biochemistry 1994;33(13):3890-5.
1994: B Posner; J Smiley; I Lee; Stephen J Benkovic
Catalytic antibodies: perusing combinatorial libraries.
Trends in biochemical sciences 1994;19(4):145-50.
1994: A Marolewski; J M Smith; Stephen J Benkovic
Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli.
Biochemistry 1994;33(9):2531-7.
1994: J D Stewart; Victoria A Roberts; N R Thomas; Elizabeth D Getzoff; Stephen J Benkovic
Site-directed mutagenesis of a catalytic antibody: an arginine and a histidine residue play key roles.
Biochemistry 1994;33(8):1994-2003.
1994: V Gopalakrishnan; Stephen J Benkovic
Effect of a thiobenzimidazolone derivative on DNA strand transfer catalyzed by HIV-1 reverse transcriptase.
The Journal of biological chemistry 1994;269(6):4110-5.
1994: Victoria A Roberts; J Stewart; Stephen J Benkovic; Elizabeth D Getzoff
Catalytic antibody model and mutagenesis implicate arginine in transition-state stabilization.
Journal of molecular biology 1994;235(3):1098-116.
1994: Christopher J Falzone; Peter Wright; Stephen J Benkovic
Dynamics of a flexible loop in dihydrofolate reductase from Escherichia coli and its implication for catalysis.
Biochemistry 1994;33(2):439-42.
1993: R T Carr; Stephen J Benkovic
An examination of the copper requirement of phenylalanine hydroxylase from Chromobacterium violaceum.
Biochemistry 1993;32(51):14132-8.
1993: B F Kaboord; Stephen J Benkovic
Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(22):10881-5.
1993: B Posner; I Lee; T Itoh; J Pyati; R Graff; george thornton; R La Polla; Stephen J Benkovic
A revised strategy for cloning antibody gene fragments in bacteria.
Gene 1993;128(1):111-7.
1993: B S Gibbs; Don M Wojchowski; Stephen J Benkovic
Expression of rat liver phenylalanine hydroxylase in insect cells and site-directed mutagenesis of putative non-heme iron-binding sites.
The Journal of biological chemistry 1993;268(11):8046-52.
1993: M W Frey; N G Nossal; T L Capson; Stephen J Benkovic
Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(7):2579-83.
1993: J D Stewart; Stephen J Benkovic
Recent developments in catalytic antibodies.
International reviews of immunology 1993;10(2-3):229-40.
1993: S Balasubramanian; R T Carr; C J Bender; Jack Peisach; Stephen J Benkovic
Histidines 138 and 143 are copper binding ligands in Chromobacterium violaceum phenylalanine hydroxylase.
Advances in experimental medicine and biology 1993;338():67-70.
1993: R T Carr; Stephen J Benkovic
A re-examination of the metal requirement of Chromobacterium violaceum phenylalanine hydroxylase.
Advances in experimental medicine and biology 1993;338():63-6.
1992: T L Capson; J A Peliska; B F Kaboord; M W Frey; C Lively; M Dahlberg; Stephen J Benkovic
Kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage T4.
Biochemistry 1992;31(45):10984-94.
1992: V Gopalakrishnan; J A Peliska; Stephen J Benkovic
Human immunodeficiency virus type 1 reverse transcriptase: spatial and temporal relationship between the polymerase and RNase H activities.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(22):10763-7.
1992: J A Peliska; Stephen J Benkovic
Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
Science (New York, N.Y.) 1992;258(5085):1112-8.
1992: Richard A Gibbs; S Taylor; Stephen J Benkovic
Antibody-catalyzed rearrangement of the peptide bond.
Science (New York, N.Y.) 1992;258(5083):803-5.
1992: J A Peliska; Stephen J Benkovic
A handy enzyme for transcription.
Current biology : CB 1992;2(10):521-3.
1992: B T Eger; Stephen J Benkovic
Minimal kinetic mechanism for misincorporation by DNA polymerase I (Klenow fragment).
Biochemistry 1992;31(38):9227-36.
1992: P Chen; U Schulze-Gahmen; Enrico STURA; James Inglese; D L Johnson; A Marolewski; Stephen J Benkovic; Ian A Wilson
Crystal structure of glycinamide ribonucleotide transformylase from Escherichia coli at 3.0 A resolution. A target enzyme for chemotherapy.
Journal of molecular biology 1992;227(1):283-92.
1992: C R Wagner; J Thillet; Stephen J Benkovic
Complementary perturbation of the kinetic mechanism and catalytic effectiveness of dihydrofolate reductase by side-chain interchange.
Biochemistry 1992;31(34):7834-40.
1992: L Li; Christopher J Falzone; Peter Wright; Stephen J Benkovic
Functional role of a mobile loop of Escherichia coli dihydrofolate reductase in transition-state stabilization.
Biochemistry 1992;31(34):7826-33.
1992: C R Wagner; Stephen J Benkovic
Probing the molecular basis of resistance to pyrimethamine by site-directed mutagenesis.
Journal of medicinal chemistry 1992;35(15):2912-5.
1992: A H Polesky; M E Dahlberg; Stephen J Benkovic; Nigel D F Grindley; Catherine M Joyce
Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.
The Journal of biological chemistry 1992;267(12):8417-28.
1992: W D Huse; L Sastry; S A Iverson; A S Kang; M Alting-Mees; D R Burton; Stephen J Benkovic; Richard A Lerner
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. 1989.
Biotechnology (Reading, Mass.) 1992;24():517-23.
1991: A Onishi; L J Liotta; Stephen J Benkovic
Cloning and expression of Chromobacterium violaceum phenylalanine hydroxylase in Escherichia coli and comparison of amino acid sequence with mammalian aromatic amino acid hydroxylases.
The Journal of biological chemistry 1991;266(28):18454-9.
1991: Carlos F Barbas; Angray S Kang; Richard A Lerner; Stephen J Benkovic
Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(18):7978-82.
1991: C R Guest; R A Hochstrasser; C G Dupuy; D J Allen; Stephen J Benkovic; David P Millar
Interaction of DNA with the Klenow fragment of DNA polymerase I studied by time-resolved fluorescence spectroscopy.
Biochemistry 1991;30(36):8759-70.
1991: M Cowart; Stephen J Benkovic; H A Nash
Behavior of a cross-linked attachment site: testing the role of branch migration in site-specific recombination.
Journal of molecular biology 1991;220(3):621-9.
1991: B S Gibbs; Stephen J Benkovic
Affinity labeling of the active site and the reactive sulfhydryl associated with activation of rat liver phenylalanine hydroxylase.
Biochemistry 1991;30(27):6795-802.
1991: M E Dahlberg; Stephen J Benkovic
Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant.
Biochemistry 1991;30(20):4835-43.
1991: Angray S Kang; Carlos F Barbas; Kim D Janda; Stephen J Benkovic; Richard A Lerner
Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(10):4363-6.
1991: Peter Wirsching; J A Ashley; Stephen J Benkovic; Kim D Janda; Richard A Lerner
An unexpectedly efficient catalytic antibody operating by ping-pong and induced fit mechanisms.
Science (New York, N.Y.) 1991;252(5006):680-5.
1991: Richard A Lerner; Stephen J Benkovic; P G Schultz
At the crossroads of chemistry and immunology: catalytic antibodies.
Science (New York, N.Y.) 1991;252(5006):659-67.
1991: Richard A Gibbs; B A Posner; D R Filpula; S W Dodd; Malcolm Finkelman; T K Lee; M Wroble; M Whitlow; Stephen J Benkovic
Construction and characterization of a single-chain catalytic antibody.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(9):4001-4.
1991: T L Capson; Stephen J Benkovic; N G Nossal
Protein-DNA cross-linking demonstrates stepwise ATP-dependent assembly of T4 DNA polymerase and its accessory proteins on the primer-template.
Cell 1991;65(2):249-58.
1991: Christopher J Falzone; Peter Wright; Stephen J Benkovic
Evidence for two interconverting protein isomers in the methotrexate complex of dihydrofolate reductase from Escherichia coli.
Biochemistry 1991;30(8):2184-91.
1991: L Y Li; Stephen J Benkovic
Impact on catalysis of secondary structural manipulation of the alpha C-helix of Escherichia coli dihydrofolate reductase.
Biochemistry 1991;30(6):1470-8.
1991: B T Eger; R D Kuchta; S S Carroll; P A Benkovic; M E Dahlberg; Catherine M Joyce; Stephen J Benkovic
Mechanism of DNA replication fidelity for three mutants of DNA polymerase I: Klenow fragment KF(exo+), KF(polA5), and KF(exo-).
Biochemistry 1991;30(5):1441-8.
1991: M Cowart; Stephen J Benkovic
A novel combined chemical-enzymatic synthesis of cross-linked DNA using a nucleoside triphosphate analogue.
Biochemistry 1991;30(3):788-96.
1991: S S Carroll; M Cowart; Stephen J Benkovic
A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity.
Biochemistry 1991;30(3):804-13.
1991: Stephen J Benkovic; J Adams; K D Janda; R A Lerner
A catalytic antibody uses a multistep kinetic sequence.
Ciba Foundation symposium 1991;159():4-9; discussion 9-12.
1991: L Sastry; M Mubaraki; K D Janda; Stephen J Benkovic; Richard A Lerner
Screening combinatorial antibody libraries for catalytic acyl transfer reactions.
Ciba Foundation symposium 1991;159():145-51; discussion 151-5.
1991: Stephen J Benkovic
Catalytic antibodies.
Harvey lectures 1991;87():115-28.
1990: Stephen J Benkovic; J A Adams; C L Borders; K D Janda; R A Lerner
The enzymic nature of antibody catalysis: development of multistep kinetic processing.
Science (New York, N.Y.) 1990;250(4984):1135-9.
1990: Christopher J Falzone; Stephen J Benkovic; Peter Wright
Partial 1H NMR assignments of the Escherichia coli dihydrofolate reductase complex with folate: evidence for a unique conformation of bound folate.
Biochemistry 1990;29(41):9667-77.
1990: Victoria A Roberts; B L Iverson; S A Iverson; Stephen J Benkovic; Richard A Lerner; Elizabeth D Getzoff; John A Tainer
Antibody remodeling: a general solution to the design of a metal-coordination site in an antibody binding pocket.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(17):6654-8.
1990: C R Wagner; Stephen J Benkovic
Site directed mutagenesis: a tool for enzyme mechanism dissection.
Trends in biotechnology 1990;8(9):263-70.
1990: D L Johnson; R J Mullin; D S Duch; Stephen J Benkovic
Direct demonstration of the active salvage of preformed purines by murine tumors.
Biochemical and biophysical research communications 1990;170(3):1164-9.
1990: B L Iverson; S A Iverson; Victoria A Roberts; Elizabeth D Getzoff; John A Tainer; Stephen J Benkovic; Richard A Lerner
Metalloantibodies.
Science (New York, N.Y.) 1990;249(4969):659-62.
1990: James Inglese; J M Smith; Stephen J Benkovic
Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli.
Biochemistry 1990;29(28):6678-87.
1990: C E Catalano; D J Allen; Stephen J Benkovic
Interaction of Escherichia coli DNA polymerase I with azidoDNA and fluorescent DNA probes: identification of protein-DNA contacts.
Biochemistry 1990;29(15):3612-21.
1990: James Inglese; D L Johnson; A Shiau; J M Smith; Stephen J Benkovic
Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase.
Biochemistry 1990;29(6):1436-43.
1989: D J Allen; Stephen J Benkovic
Resonance energy transfer measurements between substrate binding sites within the large (Klenow) fragment of Escherichia coli DNA polymerase I.
Biochemistry 1989;28(25):9586-93.
1989: W D Huse; L Sastry; S A Iverson; A S Kang; M Alting-Mees; D R Burton; Stephen J Benkovic; Richard A Lerner
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.
Science (New York, N.Y.) 1989;246(4935):1275-81.
1989: J Adams; K Johnson; R Matthews; Stephen J Benkovic
Effects of distal point-site mutations on the binding and catalysis of dihydrofolate reductase from Escherichia coli.
Biochemistry 1989;28(16):6611-8.
1989: L Sastry; M Alting-Mees; W D Huse; Jay Short; J A Sorge; B N Hay; K D Janda; Stephen J Benkovic; Richard A Lerner
Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(15):5728-32.
1989: J Andrews; Carol A Fierke; Berry Birdsall; G Ostler; James Feeney; Gordon Roberts; Stephen J Benkovic
A kinetic study of wild-type and mutant dihydrofolate reductases from Lactobacillus casei.
Biochemistry 1989;28(14):5743-50.
1989: M Sulewski; S P Marchese-Ragona; Kenneth A Johnson; Stephen J Benkovic
Mechanism of polynucleotide phosphorylase.
Biochemistry 1989;28(14):5855-64.
1989: Enrico STURA; D L Johnson; James Inglese; J M Smith; Stephen J Benkovic; Ian A Wilson
Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase.
The Journal of biological chemistry 1989;264(16):9703-6.
1989: D J Allen; P L Darke; Stephen J Benkovic
Fluorescent oligonucleotides and deoxynucleotide triphosphates: preparation and their interaction with the large (Klenow) fragment of Escherichia coli DNA polymerase I.
Biochemistry 1989;28(11):4601-7.
1989: C E Catalano; Stephen J Benkovic
Inactivation of DNA polymerase I (Klenow fragment) by adenosine 2',3'-epoxide 5'-triphosphate: evidence for the formation of a tight-binding inhibitor.
Biochemistry 1989;28(10):4374-82.
1989: James Inglese; R A Blatchly; Stephen J Benkovic
A multisubstrate adduct inhibitor of a purine biosynthetic enzyme with a picomolar dissociation constant.
Journal of medicinal chemistry 1989;32(5):937-40.
1989: K D Janda; Stephen J Benkovic; Richard A Lerner
Catalytic antibodies with lipase activity and R or S substrate selectivity.
Science (New York, N.Y.) 1989;244(4903):437-40.
1989: D J Murphy; Stephen J Benkovic
Hydrophobic interactions via mutants of Escherichia coli dihydrofolate reductase: separation of binding and catalysis.
Biochemistry 1989;28(7):3025-31.
1989: S O Pember; Kenneth A Johnson; J J Villafranca; Stephen J Benkovic
Mechanistic studies on phenylalanine hydroxylase from Chromobacterium violaceum. Evidence for the formation of an enzyme-oxygen complex.
Biochemistry 1989;28(5):2124-30.
1989: M Cowart; K J Gibson; D J Allen; Stephen J Benkovic
DNA substrate structural requirements for the exonuclease and polymerase activities of procaryotic and phage DNA polymerases.
Biochemistry 1989;28(5):1975-83.
1989: S A Iverson; L Sastry; W D Huse; J A Sorge; Stephen J Benkovic; Richard A Lerner
A combinatorial system for cloning and expressing the catalytic antibody repertoire in Escherichia coli.
Cold Spring Harbor symposia on quantitative biology 1989;54 Pt 1():273-81.
1988: R D Kuchta; M Cowart; D Allen; Stephen J Benkovic
Kinetic and structural investigations of the replicative fidelity of the Klenow fragment.
Biochemical Society transactions 1988;16(6):947-9.
1988: R D Kuchta; P Benkovic; Stephen J Benkovic
Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity.
Biochemistry 1988;27(18):6716-25.
1988: K D Janda; D Schloeder; Stephen J Benkovic; Richard A Lerner
Induction of an antibody that catalyzes the hydrolysis of an amide bond.
Science (New York, N.Y.) 1988;241(4870):1188-91.
1988: M Parvez; A D Napper; Stephen J Benkovic
Structure of 6-(iodomethyl)-2-oxo-2-phenoxy-1,2-oxaphosphorinane.
Acta crystallographica. Section C, Crystal structure communications 1988;44 ( Pt 8)():1414-6.
1988: Stephen J Benkovic; A D Napper; R A Lerner
Catalysis of a stereospecific bimolecular amide synthesis by an antibody.
Proceedings of the National Academy of Sciences of the United States of America 1988;85(15):5355-8.
1988: R J Miller; Stephen J Benkovic
L-[2,5-H2]phenylalanine, an alternate substrate for rat liver phenylalanine hydroxylase.
Biochemistry 1988;27(10):3658-63.
1988: Stephen J Benkovic; Carol A Fierke; A M Naylor
Insights into enzyme function from studies on mutants of dihydrofolate reductase.
Science (New York, N.Y.) 1988;239(4844):1105-10.
1988: W T Mueller; G K Smith; Stephen J Benkovic; J B Hynes
Inhibition of chicken liver 5-aminoimidazole-4-carboxamide ribonucleotide transformylase by 5,8-dideaza analogues of folic acid.
Biochemical pharmacology 1988;37(3):449-51.
1988: K Taira; Stephen J Benkovic
Evaluation of the importance of hydrophobic interactions in drug binding to dihydrofolate reductase.
Journal of medicinal chemistry 1988;31(1):129-37.
1988: V Mizrahi; Stephen J Benkovic
The dynamics of DNA polymerase-catalyzed reactions.
Advances in enzymology and related areas of molecular biology 1988;61():437-57.
1987: R D Kuchta; V Mizrahi; P A Benkovic; Kenneth A Johnson; Stephen J Benkovic
Kinetic mechanism of DNA polymerase I (Klenow).
Biochemistry 1987;26(25):8410-7.
1987: S O Pember; Stephen J Benkovic; J J Villafranca; M Pasenkiewicz-Gierula; William E Antholine
Adduct formation between the cupric site of phenylalanine hydroxylase from Chromobacterium violaceum and 6,7-dimethyltetrahydropterin.
Biochemistry 1987;26(14):4477-83.
1987: J T Chen; K Taira; C P Tu; Stephen J Benkovic
Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis.
Biochemistry 1987;26(13):4093-100.
1987: Carol A Fierke; Kenneth A Johnson; Stephen J Benkovic
Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli.
Biochemistry 1987;26(13):4085-92.
1987: Thomas A Dix; Donald M Kuhn; Stephen J Benkovic
Mechanism of oxygen activation by tyrosine hydroxylase.
Biochemistry 1987;26(12):3354-61.
1987: S O Pember; J J Villafranca; Stephen J Benkovic
Chromobacterium violaceum phenylalanine 4-monooxygenase.
Methods in enzymology 1987;142():50-6.
1987: Alfonso Tramontano; K Janda; A D Napper; Stephen J Benkovic; Richard A Lerner
Catalytic antibodies.
Cold Spring Harbor symposia on quantitative biology 1987;52():91-6.
1987: Carol A Fierke; R D Kuchta; Kenneth A Johnson; Stephen J Benkovic
Implications for enzymic catalysis from free-energy reaction coordinate profiles.
Cold Spring Harbor symposia on quantitative biology 1987;52():631-8.
1986: S O Pember; J J Villafranca; Stephen J Benkovic
Phenylalanine hydroxylase from Chromobacterium violaceum is a copper-containing monooxygenase. Kinetics of the reductive activation of the enzyme.
Biochemistry 1986;25(21):6611-9.
1986: V Mizrahi; P Benkovic; Stephen J Benkovic
Mechanism of DNA polymerase I: exonuclease/polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(16):5769-73.
1986: V Mizrahi; P A Benkovic; Stephen J Benkovic
Mechanism of the idling-turnover reaction of the large (Klenow) fragment of Escherichia coli DNA polymerase I.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(2):231-5.
1984: Gail Folena-Wasserman; W T Mueller; Stephen J Benkovic; W S Liao; J Taylor
Evidence that the folate-requiring enzymes of de novo purine biosynthesis are encoded by individual mRNAs.
Biochemistry 1984;23(26):6704-10.
1982: G K Smith; W T Mueller; L J Slieker; C W DeBrosse; Stephen J Benkovic
Direct transfer of one-carbon units in the transformylations of de novo purine biosynthesis.
Biochemistry 1982;21(12):2870-4.
1981: G K Smith; W T Mueller; P A Benkovic; Stephen J Benkovic
On the cofactor specificity of glycinamide ribonucleotide and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase from chicken liver.
Biochemistry 1981;20(5):1241-5.
1980: G K Smith; W T Mueller; Gail Folena-Wasserman; W D Taylor; Stephen J Benkovic
Characterization of the enzyme complex involving the folate-requiring enzymes of de novo purine biosynthesis.
Biochemistry 1980;19(18):4313-21.