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Eleftherios Papoutsakis

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Swapna Panuganti; Eleftherios T Papoutsakis; Alaina C Schlinker; Paul F Lindholm; William M Miller
Three-stage ex vivo expansion of high-ploidy megakaryocytic cells: toward large-scale platelet production.
Jinlin Jiang; Eleftherios T Papoutsakis
Stem-cell niche based comparative analysis of chemical and nano-mechanical material properties impacting ex vivo expansion and differentiation of hematopoietic and mesenchymal stem cells.
Stephan Lindsey; Eleftherios T Papoutsakis
The evolving role of the aryl hydrocarbon receptor (AHR) in the normophysiology of hematopoiesis.

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2013: Swapna Panuganti; Eleftherios T Papoutsakis; Alaina C Schlinker; Paul F Lindholm; William M Miller
Three-stage ex vivo expansion of high-ploidy megakaryocytic cells: toward large-scale platelet production.
Tissue engineering. Part A 2013;19(7-8):998-1014.
2013: Jinlin Jiang; Eleftherios T Papoutsakis
Stem-cell niche based comparative analysis of chemical and nano-mechanical material properties impacting ex vivo expansion and differentiation of hematopoietic and mesenchymal stem cells.
Advanced healthcare materials 2013;2(1):25-42.
2012: Stephan Lindsey; Eleftherios T Papoutsakis
The evolving role of the aryl hydrocarbon receptor (AHR) in the normophysiology of hematopoiesis.
Stem cell reviews 2012;8(4):1223-35.
2012: Mohab A Al-Hinai; Alan G Fast; Eleftherios T Papoutsakis
Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.
Applied and environmental microbiology 2012;78(22):8112-21.
2012: Sergios A Nicolaou; Stefan M Gaida; Eleftherios T Papoutsakis
Exploring the combinatorial genomic space in Escherichia coli for ethanol tolerance.
Biotechnology journal 2012;7(11):1337-45.
2012: Pani A Apostolidis; Stephan Lindsey; William M Miller; Eleftherios T Papoutsakis
Proposed megakaryocytic regulon of p53: the genes engaged to control cell cycle and apoptosis during megakaryocytic differentiation.
Physiological genomics 2012;44(12):638-50.
2012: Bryan P Tracy; Alan G Fast; Dinesh C Indurthi; Shawn W Jones; Eleftherios T Papoutsakis
Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications.
Current opinion in biotechnology 2012;23(3):364-81.
2011: Changhao Bi; Daniel R Hess; Shawn W Jones; Bryan P Tracy; Eleftherios T Papoutsakis
SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824.
Journal of bacteriology 2011;193(19):5130-7.
2011: Shawn W Jones; Bryan P Tracy; Stefan M Gaida; Eleftherios T Papoutsakis
Inactivation of σF in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes σE and σG protein expression but does not block solvent formation.
Journal of bacteriology 2011;193(10):2429-40.
2011: Scott B Crown; Dinesh C Indurthi; Woo Suk Ahn; Jungik Choi; Eleftherios T Papoutsakis; Maciek R Antoniewicz
Resolving the TCA cycle and pentose-phosphate pathway of Clostridium acetobutylicum ATCC 824: Isotopomer analysis, in vitro activities and expression analysis.
Biotechnology journal 2011;6(3):300-5.
2011: Bryan P Tracy; Shawn W Jones; Eleftherios T Papoutsakis
Inactivation of σE and σG in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis.
Journal of bacteriology 2011;193(6):1414-26.
2011: Stephan Lindsey; Eleftherios T Papoutsakis
The aryl hydrocarbon receptor (AHR) transcription factor regulates megakaryocytic polyploidization.
British journal of haematology 2011;152(4):469-84.
2011: Yili Chen; Dinesh C Indurthi; Shawn W Jones; Eleftherios T Papoutsakis
Small RNAs in the genus Clostridium.
mBio 2011;2(1):e00340-10.
2010: Swapna Panuganti; Eleftherios T Papoutsakis; William M Miller
Bone marrow niche-inspired, multiphase expansion of megakaryocytic progenitors with high polyploidization potential.
Cytotherapy 2010;12(6):767-82.
2010: Sergios A Nicolaou; Stefan M Gaida; Eleftherios T Papoutsakis
A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: From biofuels and chemicals, to biocatalysis and bioremediation.
Metabolic engineering 2010;12(4):307-31.
2010: Jacob R Borden; Shawn W Jones; Dinesh Indurthi; Yili Chen; Eleftherios T Papoutsakis
A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing.
Metabolic engineering 2010;12(3):268-81.
2010: Keith V Alsaker; Carlos J Paredes; Eleftherios T Papoutsakis
Metabolite stress and tolerance in the production of biofuels and chemicals: gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum.
Biotechnology and bioengineering 2010;105(6):1131-47.
2010: Bryan P Tracy; Stefan M Gaida; Eleftherios T Papoutsakis
Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes.
Current opinion in biotechnology 2010;21(1):85-99.
2009: Lisa M Giammona; Swapna Panuganti; Jan M Kemper; Pani A Apostolidis; Stephan Lindsey; Eleftherios T Papoutsakis; William M Miller
Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition.
Experimental hematology 2009;37(11):1340-1352.e3.
2009: Min Wang; Ryan S Senger; Carlos J Paredes; Gautam G Banik; Andy Lin; Eleftherios T Papoutsakis
Microarray-based gene expression analysis as a process characterization tool to establish comparability of complex biological products: scale-up of a whole-cell immunotherapy product.
Biotechnology and bioengineering 2009;104(4):796-808.
2009: Kurt T Kunas; Eleftherios T Papoutsakis
Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment. Biotechnol Bioeng 1990; 36:476-83.
Biotechnology and bioengineering 2009;102(4):980-87; discussion 977-9.
2009: Ryan Sillers; Mohab Ali Al-Hinai; Eleftherios T Papoutsakis
Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations.
Biotechnology and bioengineering 2009;102(1):38-49.
2008: Bryan P Tracy; Stefan M Gaida; Eleftherios T Papoutsakis
Development and application of flow-cytometric techniques for analyzing and sorting endospore-forming clostridia.
Applied and environmental microbiology 2008;74(24):7497-506.
2008: Ryan S Senger; Eleftherios T Papoutsakis
Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis.
Biotechnology and bioengineering 2008;101(5):1036-52.
2008: Ryan S Senger; Eleftherios T Papoutsakis
Genome-scale model for Clostridium acetobutylicum: Part II. Development of specific proton flux states and numerically determined sub-systems.
Biotechnology and bioengineering 2008;101(5):1053-71.
2008: Ryan Sillers; Alison Chow; Bryan Tracy; Eleftherios T Papoutsakis
Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance.
Metabolic engineering 2008;10(6):321-32.
2008: Eleftherios T Papoutsakis
Engineering solventogenic clostridia.
Current opinion in biotechnology 2008;19(5):420-9.
2008: Peter G Fuhrken; Pani A Apostolidis; Stephan Lindsey; William M Miller; Eleftherios T Papoutsakis
Tumor suppressor protein p53 regulates megakaryocytic polyploidization and apoptosis.
The Journal of biological chemistry 2008;283(23):15589-600.
2008: Peter G Fuhrken; Chi Chen; Pani A Apostolidis; Min Wang; William M Miller; Eleftherios T Papoutsakis
Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis.
Physiological genomics 2008;33(2):159-69.
2008: Shawn W Jones; Carlos J Paredes; Bryan Tracy; Nathan Cheng; Ryan Sillers; Ryan S Senger; Eleftherios T Papoutsakis
The transcriptional program underlying the physiology of clostridial sporulation.
Genome biology 2008;9(7):R114.
2008: Min Wang; Dirk Windgassen; Eleftherios T Papoutsakis
Comparative analysis of transcriptional profiling of CD3+, CD4+ and CD8+ T cells identifies novel immune response players in T-cell activation.
BMC genomics 2008;9():225.
2007: Deborah E Pascoe; David Arnott; Eleftherios T Papoutsakis; William M Miller; Dana C Andersen
Proteome analysis of antibody-producing CHO cell lines with different metabolic profiles.
Biotechnology and bioengineering 2007;98(2):391-410.
2007: Li Ting Huang; Carlos J Paredes; Eleftherios T Papoutsakis; William M Miller
Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytes.
Physiological genomics 2007;31(1):114-25.
2007: Carlos J Paredes; Ryan S Senger; Iwona S Spath; Jacob R Borden; Ryan Sillers; Eleftherios T Papoutsakis
A general framework for designing and validating oligomer-based DNA microarrays and its application to Clostridium acetobutylicum.
Applied and environmental microbiology 2007;73(14):4631-8.
2007: Hao Wang; YuFeng Lu; Weiqi Huang; Eleftherios T Papoutsakis; Peter Fuhrken; Elizabeth A Eklund
HoxA10 activates transcription of the gene encoding mitogen-activated protein kinase phosphatase 2 (Mkp2) in myeloid cells.
The Journal of biological chemistry 2007;282(22):16164-76.
2007: Jacob R Borden; Eleftherios T Papoutsakis
Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum.
Applied and environmental microbiology 2007;73(9):3061-8.
2007: Peter G Fuhrken; Chi Chen; William M Miller; Eleftherios T Papoutsakis
Comparative, genome-scale transcriptional analysis of CHRF-288-11 and primary human megakaryocytic cell cultures provides novel insights into lineage-specific differentiation.
Experimental hematology 2007;35(3):476-489.
2007: Christopher G Ramsborg; Eleftherios T Papoutsakis
Global transcriptional analysis delineates the differential inflammatory response interleukin-15 elicits from cultured human T cells.
Experimental hematology 2007;35(3):454-464.
2007: Dipankar Ray; Yasuhisa Terao; Peter G Fuhrken; Zhi-Qing Ma; Francesco J DeMayo; Konstantin Christov-Tzelkov; Nyla A Heerema; Roberta Franks; Sophia Y Tsai; Eleftherios T Papoutsakis; Hiroaki Kiyokawa
Deregulated CDC25A expression promotes mammary tumorigenesis with genomic instability.
Cancer research 2007;67(3):984-91.
2007: Chi Chen; Peter G Fuhrken; Li Ting Huang; Pani Apostolidis; Min Wang; Carlos J Paredes; William M Miller; Eleftherios T Papoutsakis
A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis.
BMC genomics 2007;8():384.
2007: Reuben Thomas; Carlos J Paredes; Sanjay Mehrotra; Vassily Hatzimanikatis; Eleftherios T Papoutsakis
A model-based optimization framework for the inference of regulatory interactions using time-course DNA microarray expression data.
BMC bioinformatics 2007;8():228.
2006: Lisa M Giammona; Peter G Fuhrken; Eleftherios T Papoutsakis; William M Miller
Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytes.
British journal of haematology 2006;135(4):554-66.
2005: Carlos J Paredes; Keith V Alsaker; Eleftherios T Papoutsakis
A comparative genomic view of clostridial sporulation and physiology.
Nature reviews. Microbiology 2005;3(12):969-78.
2005: Jacob R Borden; Carlos J Paredes; Eleftherios T Papoutsakis
Diffusion, mixing, and associated dye effects in DNA-microarray hybridizations.
Biophysical journal 2005;89(5):3277-84.
2005: Keith V Alsaker; Eleftherios T Papoutsakis
Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum.
Journal of bacteriology 2005;187(20):7103-18.
2005: Yinsuo Zhao; Christopher A Tomas; Frederick B Rudolph; Eleftherios T Papoutsakis; george bennett
Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation.
Applied and environmental microbiology 2005;71(1):530-7.
2004: Reuben Thomas; Sanjay Mehrotra; Eleftherios T Papoutsakis; Vassily Hatzimanikatis
A model-based optimization framework for the inference on gene regulatory networks from DNA array data.
Bioinformatics (Oxford, England) 2004;20(17):3221-35.
2004: Christopher G Ramsborg; Dirk Windgassen; Jonathan K Fallon; Carlos J Paredes; Eleftherios T Papoutsakis
Molecular insights into the pleiotropic effects of plasma on ex vivo-expanded T cells using DNA-microarray analysis.
Experimental hematology 2004;32(10):970-90.
2004: Hadar Haddad; Dirk Windgassen; Christopher G Ramsborg; Carlos J Paredes; Eleftherios T Papoutsakis
Molecular understanding of oxygen-tension and patient-variability effects on ex vivo expanded T cells.
Biotechnology and bioengineering 2004;87(4):437-50.
2004: Christopher A Tomas; Jeffrey Beamish; Eleftherios T Papoutsakis
Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum.
Journal of bacteriology 2004;186(7):2006-18.
2004: Keith V Alsaker; Thomas R Spitzer; Eleftherios T Papoutsakis
Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress.
Journal of bacteriology 2004;186(7):1959-71.
2004: Carlos J Paredes; Isidore Rigoutsos; Eleftherios T Papoutsakis
Transcriptional organization of the Clostridium acetobutylicum genome.
Nucleic acids research 2004;32(6):1973-81.
2003: Seshu B Tummala; Stefan G Junne; Carlos J Paredes; Eleftherios T Papoutsakis
Transcriptional analysis of product-concentration driven changes in cellular programs of recombinant Clostridium acetobutylicumstrains.
Biotechnology and bioengineering 2003;84(7):842-54.
2003: Eleftherios T Papoutsakis
Murray Moo-Young: the gentleman of biochemical engineering.
Biotechnology advances 2003;21(5):381-2.
2003: Christopher A Tomas; Neil E Welker; Eleftherios T Papoutsakis
Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program.
Applied and environmental microbiology 2003;69(8):4951-65.
2003: Christopher A Tomas; Keith V Alsaker; Hendrik P J Bonarius; Wouter T Hendriksen; He Yang; Jeffrey A Beamish; Carlos J Paredes; Eleftherios T Papoutsakis
DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5.
Journal of bacteriology 2003;185(15):4539-47.
2003: Seshu B Tummala; Stefan G Junne; Eleftherios T Papoutsakis
Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations.
Journal of bacteriology 2003;185(12):3644-53.
2003: Seshu B Tummala; Neil E Welker; Eleftherios T Papoutsakis
Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum.
Journal of bacteriology 2003;185(6):1923-34.
2003: He Yang; Hadar Haddad; Christopher A Tomas; Keith V Alsaker; Eleftherios T Papoutsakis
A segmental nearest neighbor normalization and gene identification method gives superior results for DNA-array analysis.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(3):1122-7.
2002: Latonia M Harris; Neil E Welker; Eleftherios T Papoutsakis
Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824.
Journal of bacteriology 2002;184(13):3586-97.
2002: Diane L Hevehan; William M Miller; Eleftherios T Papoutsakis
Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiation.
Blood 2002;99(5):1627-37.
2002: H Yang; William M Miller; Eleftherios T Papoutsakis
Higher pH promotes megakaryocytic maturation and apoptosis.
Stem cells (Dayton, Ohio) 2002;20(4):320-8.
2001: Latonia M Harris; Lars Blank; RP Desai; Neil E Welker; Eleftherios T Papoutsakis
Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene.
Journal of industrial microbiology & biotechnology 2001;27(5):322-8.
2001: D C Chow; LA Wenning; William M Miller; Eleftherios T Papoutsakis
Modeling pO(2) distributions in the bone marrow hematopoietic compartment. II. Modified Kroghian models.
Biophysical journal 2001;81(2):685-96.
2001: D C Chow; LA Wenning; William M Miller; Eleftherios T Papoutsakis
Modeling pO(2) distributions in the bone marrow hematopoietic compartment. I. Krogh's model.
Biophysical journal 2001;81(2):675-84.
2001: S S Mostafa; Eleftherios T Papoutsakis; William M Miller
Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytes.
Experimental hematology 2001;29(7):873-83.
2001: H Yang; Eleftherios T Papoutsakis; William M Miller
Model-based estimation of myeloid hematopoietic progenitor cells in ex vivo cultures for cell and gene therapies.
Biotechnology and bioengineering 2001;72(2):144-55.
2001: H Haddad; Eleftherios T Papoutsakis
Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Cytotherapy 2001;3(6):435-47.
2000: S S Mostafa; William M Miller; Eleftherios T Papoutsakis
Oxygen tension influences the differentiation, maturation and apoptosis of human megakaryocytes.
British journal of haematology 2000;111(3):879-89.
2000: KS Carswell; Eleftherios T Papoutsakis
Extracellular pH affects the proliferation of cultured human T cells and their expression of the interleukin-2 receptor.
Journal of immunotherapy (Hagerstown, Md. : 1997) 2000;23(6):669-74.
2000: SD Patel; Eleftherios T Papoutsakis; JN Winter; William M Miller
The lactate issue revisited: novel feeding protocols to examine inhibition of cell proliferation and glucose metabolism in hematopoietic cell cultures.
Biotechnology progress 2000;16(5):885-92.
2000: Diane L Hevehan; LA Wenning; William M Miller; Eleftherios T Papoutsakis
Dynamic model of ex vivo granulocytic kinetics to examine the effects of oxygen tension, pH, and interleukin-3.
Experimental hematology 2000;28(9):1016-28.
2000: KS Carswell; Eleftherios T Papoutsakis
Culture of human T cells in stirred bioreactors for cellular immunotherapy applications: shear, proliferation, and the IL-2 receptor.
Biotechnology and bioengineering 2000;68(3):328-38.
2000: Diane L Hevehan; Eleftherios T Papoutsakis; William M Miller
Physiologically significant effects of pH and oxygen tension on granulopoiesis.
Experimental hematology 2000;28(3):267-75.
2000: Latonia M Harris; RP Desai; Neil E Welker; Eleftherios T Papoutsakis
Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: need for new phenomenological models for solventogenesis and butanol inhibition?
Biotechnology and bioengineering 2000;67(1):1-11.
2000: KS Carswell; J W Weiss; Eleftherios T Papoutsakis
Low oxygen tension enhances the stimulation and proliferation of human T lymphocytes in the presence of IL-2.
Cytotherapy 2000;2(1):25-37.
2000: SD Patel; R Guo; William M Miller; Eleftherios T Papoutsakis; N I Minster; C M Baum; JN Winter
Clinical-scale production of granulocyte progenitor and post-progenitor cells using daniplestim, leridistim, Progenipoietin, Promegapoietin and autologous plasma.
Cytotherapy 2000;2(2):85-94.
2000: SD Patel; William M Miller; JN Winter; Eleftherios T Papoutsakis
Cell density-dependent proliferation in frequently-fed peripheral blood mononuclear cell cultures.
Cytotherapy 2000;2(4):267-80.
1999: Seshu B Tummala; Neil E Welker; Eleftherios T Papoutsakis
Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824.
Applied and environmental microbiology 1999;65(9):3793-9.
1999: RP Desai; Latonia M Harris; Neil E Welker; Eleftherios T Papoutsakis
Metabolic flux analysis elucidates the importance of the acid-formation pathways in regulating solvent production by Clostridium acetobutylicum.
Metabolic engineering 1999;1(3):206-13.
1999: RP Desai; Lars Nielsen; Eleftherios T Papoutsakis
Stoichiometric modeling of Clostridium acetobutylicum fermentations with non-linear constraints.
Journal of biotechnology 1999;71(1-3):191-205.
1999: RP Desai; Eleftherios T Papoutsakis
Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum.
Applied and environmental microbiology 1999;65(3):936-45.
1999: P C Collins; S D Patel; William M Miller; Eleftherios T Papoutsakis
Initiation, maintenance, and quantification of human hematopoietic cell cultures.
Methods in molecular medicine 1999;18():271-92.
1999: P C Collins; SD Patel; Eleftherios T Papoutsakis; William M Miller
Nuclei-size distributions as predictive tools of hematopoietic cell proliferation.
Cytotherapy 1999;1(2):99-109.
1999: R V Nair; E M Green; DE Watson; george bennett; Eleftherios T Papoutsakis
Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor.
Journal of bacteriology 1999;181(1):319-30.
1998: TA McAdams; William M Miller; Eleftherios T Papoutsakis
pH is a potent modulator of erythroid differentiation.
British journal of haematology 1998;103(2):317-25.
1998: CL McDowell; Eleftherios T Papoutsakis
Serum increases the CD13 receptor expression, reduces the transduction of fluid-mechanical forces, and alters the metabolism of HL60 cells cultured in agitated bioreactors.
Biotechnology and bioengineering 1998;60(2):259-68.
1998: CL McDowell; R T Carver; Eleftherios T Papoutsakis
Effects of methocel A15LV, polyethylene glycol, and polyvinyl alcohol on CD13 and CD33 receptor surface content and metabolism of HL60 cells cultured in stirred tank bioreactors.
Biotechnology and bioengineering 1998;60(2):251-8.
1998: CL McDowell; Eleftherios T Papoutsakis
Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in stirred tank bioreactors.
Biotechnology and bioengineering 1998;60(2):239-50.
1998: P C Collins; William M Miller; Eleftherios T Papoutsakis
Stirred culture of peripheral and cord blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications.
Biotechnology and bioengineering 1998;59(5):534-43.
1998: M Horner; William M Miller; Julio M Ottino; Eleftherios T Papoutsakis
Transport in a grooved perfusion flat-bed bioreactor for cell therapy applications.
Biotechnology progress 1998;14(5):689-98.
1998: JA LaIuppa; Eleftherios T Papoutsakis; William M Miller
Oxygen tension alters the effects of cytokines on the megakaryocyte, erythrocyte, and granulocyte lineages.
Experimental hematology 1998;26(9):835-43.
1998: CL McDowell; Eleftherios T Papoutsakis
Decreasing extracellular pH increases CD13 receptor surface content and alters the metabolism of HL60 cells cultured in stirred tank bioreactors.
Biotechnology progress 1998;14(4):567-72.
1998: P C Collins; Lars Nielsen; SD Patel; Eleftherios T Papoutsakis; William M Miller
Characterization of hematopoietic cell expansion, oxygen uptake, and glycolysis in a controlled, stirred-tank bioreactor system.
Biotechnology progress 1998;14(3):466-72.
1998: L L Bermejo; Neil E Welker; Eleftherios T Papoutsakis
Expression of Clostridium acetobutylicum ATCC 824 genes in Escherichia coli for acetone production and acetate detoxification.
Applied and environmental microbiology 1998;64(3):1079-85.
1997: JA LaIuppa; TA McAdams; Eleftherios T Papoutsakis; William M Miller
Culture materials affect ex vivo expansion of hematopoietic progenitor cells.
Journal of biomedical materials research 1997;36(3):347-59.
1997: Emmanuel Cornillot; R V Nair; Eleftherios T Papoutsakis; Philippe Soucaille
The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.
Journal of bacteriology 1997;179(17):5442-7.
1997: P C Collins; Lars Nielsen; C K Wong; Eleftherios T Papoutsakis; William M Miller
Real-time method for determining the colony-forming cell content of human hematopoietic cell cultures.
Biotechnology and bioengineering 1997;55(4):693-700.
1997: TA McAdams; William M Miller; Eleftherios T Papoutsakis
Variations in culture pH affect the cloning efficiency and differentiation of progenitor cells in ex vivo haemopoiesis.
British journal of haematology 1997;97(4):889-95.
1997: JA LaIuppa; Eleftherios T Papoutsakis; William M Miller
Evaluation of cytokines for expansion of the megakaryocyte and granulocyte lineages.
Stem cells (Dayton, Ohio) 1997;15(3):198-206.
1997: JA LaIuppa; Eleftherios T Papoutsakis; William M Miller
Ex vivo expansion of hematopoietic stem and progenitor cells for transplantation.
Cancer treatment and research 1997;77():159-86.
1996: T A McAdams; J N Winter; William M Miller; Eleftherios T Papoutsakis
Hematopoietic cell culture therapies (Part II): Clinical aspects and applications.
Trends in biotechnology 1996;14(10):388-96.
1996: C E Sandstrom; P C Collins; T A McAdams; J G Bender; Eleftherios T Papoutsakis; William M Miller
Comparison of whole serum-deprived media for ex vivo expansion of hematopoietic progenitor cells from cord blood and mobilized peripheral blood mononuclear cells.
Journal of hematotherapy 1996;5(5):461-73.
1996: T A McAdams; William M Miller; Eleftherios T Papoutsakis
Hematopoietic cell culture therapies (Part I): Cell culture considerations.
Trends in biotechnology 1996;14(9):341-9.
1996: J D Michaels; A K Mallik; Eleftherios T Papoutsakis
Sparging and agitation-induced injury of cultured animals cells: Do cell-to-bubble interactions in the bulk liquid injure cells?
Biotechnology and bioengineering 1996;51(4):399-409.
1996: E M Green; Z L Boynton; L M Harris; Frederick B Rudolph; Eleftherios T Papoutsakis; george bennett
Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824.
Microbiology (Reading, England) 1996;142 ( Pt 8)():2079-86.
1996: C E Sandstrom; J G Bender; William M Miller; Eleftherios T Papoutsakis
Development of novel perfusion chamber to retain nonadherent cells and its use for comparison of human "mobilized" peripheral blood mononuclear cell cultures with and without irradiated bone marrow stroma.
Biotechnology and bioengineering 1996;50(5):493-504.
1996: P C Collins; Eleftherios T Papoutsakis; William M Miller
Ex vivo culture systems for hematopoietic cells.
Current opinion in biotechnology 1996;7(2):223-30.
1995: C E Sandstrom; J G Bender; Eleftherios T Papoutsakis; William M Miller
Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells.
Blood 1995;86(3):958-70.
1995: TA McAdams; William M Miller; J G Bender; C E Sandstrom; Eleftherios T Papoutsakis
Ex vivo expansion of primitive hematopoietic cells for cellular therapies: An overview.
Cytotechnology 1995;18(1-2):133-46.
1995: Manfred R Koller; Eleftherios T Papoutsakis
Cell adhesion in animal cell culture: physiological and fluid-mechanical implications.
Bioprocess technology 1995;20():61-110.
1994: M C Borys; Daniel I H Linzer; Eleftherios T Papoutsakis
Cell aggregation in a Chinese hamster ovary cell microcarrier culture affects the expression rate and N-linked glycosylation of recombinant mouse placental lactogen-1.
Annals of the New York Academy of Sciences 1994;745():360-71.
1994: R V Nair; Eleftherios T Papoutsakis
Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.
Journal of bacteriology 1994;176(18):5843-6.
1994: K A Walter; L D Mermelstein; Eleftherios T Papoutsakis
Studies of recombinant Clostridium acetobutylicum with increased dosages of butyrate formation genes.
Annals of the New York Academy of Sciences 1994;721():69-72.
1994: L D Mermelstein; Neil E Welker; D J Petersen; george bennett; Eleftherios T Papoutsakis
Genetic and metabolic engineering of Clostridium acetobutylicum ATCC 824.
Annals of the New York Academy of Sciences 1994;721():54-68.
1994: R V Nair; george bennett; Eleftherios T Papoutsakis
Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.
Journal of bacteriology 1994;176(3):871-85.
1993: K A Walter; R V Nair; J W Cary; george bennett; Eleftherios T Papoutsakis
Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824.
Gene 1993;134(1):107-11.
1993: M C Borys; Daniel I H Linzer; Eleftherios T Papoutsakis
Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese hamster ovary (CHO) cells.
Bio/technology (Nature Publishing Company) 1993;11(6):720-4.
1993: Sang Yup Lee; L D Mermelstein; Eleftherios T Papoutsakis
Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824.
FEMS microbiology letters 1993;108(3):319-23.
1993: L D Mermelstein; Eleftherios T Papoutsakis
In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824.
Applied and environmental microbiology 1993;59(4):1077-81.
1993: Manfred R Koller; J G Bender; William M Miller; Eleftherios T Papoutsakis
Expansion of primitive human hematopoietic progenitors in a perfusion bioreactor system with IL-3, IL-6, and stem cell factor.
Bio/technology (Nature Publishing Company) 1993;11(3):358-63.
1992: K A Walter; george bennett; Eleftherios T Papoutsakis
Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes.
Journal of bacteriology 1992;174(22):7149-58.
1992: Sang Yup Lee; L D Mermelstein; george bennett; Eleftherios T Papoutsakis
Vector construction, transformation, and gene amplification in Clostridium acetobutylicum ATCC 824.
Annals of the New York Academy of Sciences 1992;665():39-51.
1992: Manfred R Koller; J G Bender; Eleftherios T Papoutsakis; William M Miller
Beneficial effects of reduced oxygen tension and perfusion in long-term hematopoietic cultures.
Annals of the New York Academy of Sciences 1992;665():105-16.
1992: Manfred R Koller; J G Bender; Eleftherios T Papoutsakis; William M Miller
Effects of synergistic cytokine combinations, low oxygen, and irradiated stroma on the expansion of human cord blood progenitors.
Blood 1992;80(2):403-11.
1992: Manfred R Koller; J G Bender; William M Miller; Eleftherios T Papoutsakis
Reduced oxygen tension increases hematopoiesis in long-term culture of human stem and progenitor cells from cord blood and bone marrow.
Experimental hematology 1992;20(2):264-70.
1992: L D Mermelstein; Neil E Welker; george bennett; Eleftherios T Papoutsakis
Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824.
Bio/technology (Nature Publishing Company) 1992;10(2):190-5.
1992: M C Borys; Eleftherios T Papoutsakis
Formation of bridges and large cellular clumps in CHO-cell microcarrier cultures: effects of agitation, dimethyl sulfoxide and calf serum.
Cytotechnology 1992;8(3):237-48.
1991: D J Petersen; R W Welch; K A Walter; L D Mermelstein; Eleftherios T Papoutsakis; Frederick B Rudolph; george bennett
Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum.
Annals of the New York Academy of Sciences 1991;646():94-8.
1991: Eleftherios T Papoutsakis
Fluid-mechanical damage of animal cells in bioreactors.
Trends in biotechnology 1991;9(12):427-37.
1991: Eleftherios T Papoutsakis
Media additives for protecting freely suspended animal cells against agitation and aeration damage.
Trends in biotechnology 1991;9(9):316-24.
1991: J D Michaels; Eleftherios T Papoutsakis
Polyvinyl alcohol and polyethylene glycol as protectants against fluid-mechanical injury of freely-suspended animal cells (CRL 8018).
Journal of biotechnology 1991;19(2-3):241-57.
1990: K T Kunas; Eleftherios T Papoutsakis
The protective effect of serum against hydrodynamic damage of hybridoma cells in agitated and surface-aerated bioreactors.
Journal of biotechnology 1990;15(1-2):57-69.
1990: J W Cary; D J Petersen; Eleftherios T Papoutsakis; george bennett
Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli.
Applied and environmental microbiology 1990;56(6):1576-83.
1990: M H Hüsemann; Eleftherios T Papoutsakis
Effects of propionate and acetate additions on solvent production in batch cultures of Clostridium acetobutylicum.
Applied and environmental microbiology 1990;56(5):1497-500.
1990: J F Petersen; Larry V McIntire; Eleftherios T Papoutsakis
Shear sensitivity of hybridoma cells in batch, fed-batch, and continuous cultures.
Biotechnology progress 1990;6(2):114-20.
1990: J W Cary; D J Petersen; george bennett; Eleftherios T Papoutsakis
Methods for cloning key primary metabolic enzymes and ancillary proteins associated with the acetone-butanol fermentation of Clostridium acetobutylicum.
Annals of the New York Academy of Sciences 1990;589():67-81.
1989: R W Welch; Frederick B Rudolph; Eleftherios T Papoutsakis
Purification and characterization of the NADH-dependent butanol dehydrogenase from Clostridium acetobutylicum (ATCC 824).
Archives of biochemistry and biophysics 1989;273(2):309-18.
1989: D P Wiesenborn; Frederick B Rudolph; Eleftherios T Papoutsakis
Coenzyme A transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids.
Applied and environmental microbiology 1989;55(2):323-9.
1989: D P Wiesenborn; Frederick B Rudolph; Eleftherios T Papoutsakis
Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and its role in acidogenesis.
Applied and environmental microbiology 1989;55(2):317-22.
1988: D P Wiesenborn; Frederick B Rudolph; Eleftherios T Papoutsakis
Thiolase from Clostridium acetobutylicum ATCC 824 and Its Role in the Synthesis of Acids and Solvents.
Applied and environmental microbiology 1988;54(11):2717-22.
1988: J W Cary; D J Petersen; Eleftherios T Papoutsakis; george bennett
Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli.
Journal of bacteriology 1988;170(10):4613-8.
1987: Eleftherios T Papoutsakis; C M Bussineau; I M Chu; A R Diwan; M Huesemann
Transport of substrates and metabolites and their effect on cell metabolism (in butyric-acid and methylotrophic fermentations).
Annals of the New York Academy of Sciences 1987;506():24-50.