FIND

researchers

DISCOVER

relevant articles

PROMOTE

your research

now for free

Richard Perham

Research Profile

Chemicals & Drugs

Living Beings

Genes & Molecular Sequences

Amino Acid Sequences

Concepts & Ideas

Co-author Network

Publications

TOP 3
Richard N Perham
FEBS Journal and online-only publishing.
Katherine Stott; Adlina M Yusof; Richard N Perham; D Dafydd Jones
A surface loop directs conformational switching of a lipoyl domain between a folded and a novel misfolded structure.
D Dafydd Jones; Richard N Perham
The role of loop and beta-turn residues as structural and functional determinants for the lipoyl domain from the Escherichia coli 2-oxoglutarate dehydrogenase complex.

Register for free to view:

Visualized networks:

See your personal network in
sophisticated graphical views

GeoTargeted Searches:

Locate experts around the world
and connect with global collaborators

Research Profiles:

See the research activity
of experts around the globe

Geonetwork of Richard Perham (preview)

Cities where this author has publications
Cities where co-authors of this author have publications

Flash Player Required

All Publications

2013: Richard N Perham
FEBS Journal and online-only publishing.
The FEBS journal 2013;280(1):1.
2009: Katherine Stott; Adlina M Yusof; Richard N Perham; D Dafydd Jones
A surface loop directs conformational switching of a lipoyl domain between a folded and a novel misfolded structure.
Structure (London, England : 1993) 2009;17(8):1117-27.
2008: D Dafydd Jones; Richard N Perham
The role of loop and beta-turn residues as structural and functional determinants for the lipoyl domain from the Escherichia coli 2-oxoglutarate dehydrogenase complex.
The Biochemical journal 2008;409(2):357-66.
2008: Jeffrey S Lengyel; Katherine M Stott; Xiongwu Wu; Bernard Brooks; Andrea Balbo; Peter Schuck; Richard N Perham; Sriram Subramaniam; Jacqueline L S Milne
Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
Structure (London, England : 1993) 2008;16(1):93-103.
2007: René A W Frank; Amanda J Price; Fred D Northrop; Richard N Perham; Ben F Luisi
Crystal structure of the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex.
Journal of molecular biology 2007;368(3):639-51.
2007: Markus Fries; Katherine Stott; Stephen Reynolds; Richard N Perham
Distinct modes of recognition of the lipoyl domain as substrate by the E1 and E3 components of the pyruvate dehydrogenase multienzyme complex.
Journal of molecular biology 2007;366(1):132-9.
2006: Edward McManus; Ben F Luisi; Richard N Perham
Structure of a putative lipoate protein ligase from Thermoplasma acidophilum and the mechanism of target selection for post-translational modification.
Journal of molecular biology 2006;356(3):625-37.
2006: Samantha J Holland; Carolina Sanz; Richard N Perham
Identification and specificity of pilus adsorption proteins of filamentous bacteriophages infecting Pseudomonas aeruginosa.
Virology 2006;345(2):540-8.
2006: Jacqueline L S Milne; Xiongwu Wu; Mario J Borgnia; Jeffrey S Lengyel; Bernard Brooks; Dan Shi; Richard N Perham; Sriram Subramaniam
Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy.
The Journal of biological chemistry 2006;281(7):4364-70.
2005: René A W Frank; J Venkatesh Pratap; Xue Yuan Pei; Richard N Perham; Ben F Luisi
The molecular origins of specificity in the assembly of a multienzyme complex.
Structure (London, England : 1993) 2005;13(8):1119-30.
2005: Mark D Allen; R William Broadhurst; Robert G Solomon; Richard N Perham
Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy.
The FEBS journal 2005;272(1):259-68.
2004: René A W Frank; Christopher M Titman; J Venkatesh Pratap; Ben F Luisi; Richard N Perham
A molecular switch and proton wire synchronize the active sites in thiamine enzymes.
Science (New York, N.Y.) 2004;306(5697):872-6.
2003: Hyo-Il Jung; Richard N Perham
Prediction of the binding site on E1 in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
FEBS letters 2003;555(2):405-10.
2003: Hyo-Il Jung; Alan Cooper; Richard N Perham
Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
European journal of biochemistry / FEBS 2003;270(22):4488-96.
2003: Piergiuseppe De Berardinis; Rossella Sartorius; Antonella Caivano; Dina Mascolo; Gonzalo J Domingo; Giovanna Del Pozzo; Muriel Gaubin; Richard N Perham; Dominique Piatier-Tonneau; John Guardiola
Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection.
Current HIV research 2003;1(4):441-6.
2003: David J Brockwell; Emanuele Paci; Rebecca C Zinober; Godfrey S Beddard; Peter D Olmsted; D Alastair Smith; Richard N Perham; Sheena E Radford
Pulling geometry defines the mechanical resistance of a beta-sheet protein.
Nature structural biology 2003;10(9):731-7.
2003: Markus Fries; Hyo-Il Jung; Richard N Perham
Reaction mechanism of the heterotetrameric (alpha2beta2) E1 component of 2-oxo acid dehydrogenase multienzyme complexes.
Biochemistry 2003;42(23):6996-7002.
2003: Gonzalo J Domingo; Antonella Caivano; Rossella Sartorius; Pasquale Barba; Malin Bäckström; Dominique Piatier-Tonneau; John Guardiola; Piergiuseppe De Berardinis; Richard N Perham
Induction of specific T-helper and cytolytic responses to epitopes displayed on a virus-like protein scaffold derived from the pyruvate dehydrogenase multienzyme complex.
Vaccine 2003;21(13-14):1502-9.
2003: Markus Fries; Hitesh J Chauhan; Gonzalo J Domingo; Hyo-Il Jung; Richard N Perham
Site-directed mutagenesis of a loop at the active site of E1 (alpha2beta2) of the pyruvate dehydrogenase complex. A possible common sequence motif.
European journal of biochemistry / FEBS 2003;270(5):861-70.
2003: Muriel Gaubin; Cristina Fanutti; Zohar Mishal; Antoine Durrbach; Piergiuseppe De Berardinis; Rossella Sartorius; Giovanna Del Pozzo; John Guardiola; Richard N Perham; Dominique Piatier-Tonneau
Processing of filamentous bacteriophage virions in antigen-presenting cells targets both HLA class I and class II peptide loading compartments.
DNA and cell biology 2003;22(1):11-8.
2002: Jacqueline L S Milne; Dan Shi; Peter B Rosenthal; Joshua S Sunshine; Gonzalo J Domingo; Xiongwu Wu; Bernard Brooks; Richard N Perham; Richard Henderson; Sriram Subramaniam
Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine.
The EMBO journal 2002;21(21):5587-98.
2002: Hyo-Il Jung; Alan Cooper; Richard N Perham
Identification of key amino acid residues in the assembly of enzymes into the pyruvate dehydrogenase complex of Bacillus stearothermophilus: a kinetic and thermodynamic analysis.
Biochemistry 2002;41(33):10446-53.
2002: Michael Witty; Carolina Sanz; Amish Shah; J Günter Grossmann; Kenji Mizuguchi; Richard N Perham; Ben F Luisi
Structure of the periplasmic domain of Pseudomonas aeruginosa TolA: evidence for an evolutionary relationship with the TonB transporter protein.
The EMBO journal 2002;21(16):4207-18.
2002: Lih-Wen Deng; Richard N Perham
Delineating the site of interaction on the pIII protein of filamentous bacteriophage fd with the F-pilus of Escherichia coli.
Journal of molecular biology 2002;319(3):603-14.
2002: Hyo-Il Jung; Simon J Bowden; Alan Cooper; Richard N Perham
Thermodynamic analysis of the binding of component enzymes in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
Protein science : a publication of the Protein Society 2002;11(5):1091-100.
2002: Richard N Perham; D Dafydd Jones; Hitesh J Chauhan; Mark J Howard
Substrate channelling in 2-oxo acid dehydrogenase multienzyme complexes.
Biochemical Society transactions 2002;30(2):47-51.
2001: K Tozawa; R William Broadhurst; Andrew R C Raine; C Fuller; A Alvarez; G Guillen; G Padron; Richard N Perham
Solution structure of the lipoyl domain of the chimeric dihydrolipoyl dehydrogenase P64K from Neisseria meningitidis.
European journal of biochemistry / FEBS 2001;268(18):4908-17.
2001: D M Pederson; LC Welsh; DA Marvin; M Sampson; Richard N Perham; M Yu; M R Slater
The protein capsid of filamentous bacteriophage PH75 from Thermus thermophilus.
Journal of molecular biology 2001;309(2):401-21.
2001: M Monette; Stanley J Opella; J Greenwood; A E Willis; Richard N Perham
Structure of a malaria parasite antigenic determinant displayed on filamentous bacteriophage determined by NMR spectroscopy: implications for the structure of continuous peptide epitopes of proteins.
Protein science : a publication of the Protein Society 2001;10(6):1150-9.
2001: Gonzalo J Domingo; S Orru'; Richard N Perham
Multiple display of peptides and proteins on a macromolecular scaffold derived from a multienzyme complex.
Journal of molecular biology 2001;305(2):259-67.
2001: D Dafydd Jones; Katherine Stott; Pedro A Reche; Richard N Perham
Recognition of the lipoyl domain is the ultimate determinant of substrate channelling in the pyruvate dehydrogenase multienzyme complex.
Journal of molecular biology 2001;305(1):49-60.
2001: John Guardiola; Piergiuseppe De Berardinis; Rossella Sartorius; Cristina Fanutti; Richard N Perham; Giovanna Del Pozzo
Phage display of epitopes from HIV-1 elicits strong cytolytic responses in vitro and in vivo.
Advances in experimental medicine and biology 2001;495():291-8.
2000: Hitesh J Chauhan; Gonzalo J Domingo; Hyo-Il Jung; Richard N Perham
Sites of limited proteolysis in the pyruvate decarboxylase component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and their role in catalysis.
European journal of biochemistry / FEBS 2000;267(24):7158-69.
2000: Pedro A Reche; Mark J Howard; R William Broadhurst; Richard N Perham
Heteronuclear NMR studies of the specificity of the post-translational modification of biotinyl domains by biotinyl protein ligase.
FEBS letters 2000;479(3):93-8.
2000: Piergiuseppe De Berardinis; Rossella Sartorius; Cristina Fanutti; Richard N Perham; Giovanna Del Pozzo; John Guardiola
Phage display of peptide epitopes from HIV-1 elicits strong cytolytic responses.
Nature biotechnology 2000;18(8):873-6.
2000: D Dafydd Jones; Katherine Stott; Mark J Howard; Richard N Perham
Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli.
Biochemistry 2000;39(29):8448-59.
2000: Mark J Howard; Hitesh J Chauhan; Gonzalo J Domingo; C Fuller; Richard N Perham
Protein-protein interaction revealed by NMR T(2) relaxation experiments: the lipoyl domain and E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
Journal of molecular biology 2000;295(4):1023-37.
2000: D Dafydd Jones; H J Horne; Pedro A Reche; Richard N Perham
Structural determinants of post-translational modification and catalytic specificity for the lipoyl domains of the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
Journal of molecular biology 2000;295(2):289-306.
2000: Richard N Perham
Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
Annual review of biochemistry 2000;69():961-1004.
1999: Gonzalo J Domingo; Hitesh J Chauhan; I A Lessard; C Fuller; Richard N Perham
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus.
European journal of biochemistry / FEBS 1999;266(3):1136-46.
1999: Pedro A Reche; Richard N Perham
Structure and selectivity in post-translational modification: attaching the biotinyl-lysine and lipoyl-lysine swinging arms in multifunctional enzymes.
The EMBO journal 1999;18(10):2673-82.
1999: E L Roberts; N Shu; Mark J Howard; R William Broadhurst; Anne Chapman-Smith; John C Wallace; T Morris; John E Cronan; Richard N Perham
Solution structures of apo and holo biotinyl domains from acetyl coenzyme A carboxylase of Escherichia coli determined by triple-resonance nuclear magnetic resonance spectroscopy.
Biochemistry 1999;38(16):5045-53.
1999: Piergiuseppe De Berardinis; Luciana D'Apice; Antonella Prisco; Maria Neve Ombra; Pasquale Barba; Giovanna Del Pozzo; S Petukhov; P Malik; Richard N Perham; John Guardiola
Recognition of HIV-derived B and T cell epitopes displayed on filamentous phages.
Vaccine 1999;17(11-12):1434-41.
1999: W M Tan; R Jelinek; Stanley J Opella; P Malik; TD Terry; Richard N Perham
Effects of temperature and Y21M mutation on conformational heterogeneity of the major coat protein (pVIII) of filamentous bacteriophage fd.
Journal of molecular biology 1999;286(3):787-96.
1999: L W Deng; P Malik; Richard N Perham
Interaction of the globular domains of pIII protein of filamentous bacteriophage fd with the F-pilus of Escherichia coli.
Virology 1999;253(2):271-7.
1998: LC Welsh; DA Marvin; Richard N Perham
Analysis of X-ray diffraction from fibres of Pf1 Inovirus (filamentous bacteriophage) shows that the DNA in the virion is not highly ordered.
Journal of molecular biology 1998;284(5):1265-71.
1998: I A Lessard; Gonzalo J Domingo; A Borges; Richard N Perham
Expression of genes encoding the E2 and E3 components of the Bacillus stearothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interaction in assembly in vitro.
European journal of biochemistry / FEBS 1998;258(2):491-501.
1998: P Malik; TD Terry; Francesca Bellintani; Richard N Perham
Factors limiting display of foreign peptides on the major coat protein of filamentous bacteriophage capsids and a potential role for leader peptidase.
FEBS letters 1998;436(2):263-6.
1998: Richard N Perham; Pedro A Reche
Swinging arms in multifunctional enzymes and the specificity of post-translational modification.
Biochemical Society transactions 1998;26(3):299-303.
1998: Mark J Howard; C Fuller; R William Broadhurst; Richard N Perham; J G Tang; J Quinn; Austin G Diamond; Stephen J Yeaman
Three-dimensional structure of the major autoantigen in primary biliary cirrhosis.
Gastroenterology 1998;115(1):139-46.
1998: Cristina Fanutti; Giovanna Del Pozzo; Piergiuseppe De Berardinis; John Guardiola; L W Deng; Richard N Perham
Phage-display of antigenic peptides applied to vaccine design.
Biochemical Society transactions 1998;26(1):S8.
1998: Pedro A Reche; Y L Li; C Fuller; K Eichhorn; Richard N Perham
Selectivity of post-translational modification in biotinylated proteins: the carboxy carrier protein of the acetyl-CoA carboxylase of Escherichia coli.
The Biochemical journal 1998;329 ( Pt 3)():589-96.
1998: LC Welsh; Martyn F Symmons; Julian M Sturtevant; DA Marvin; Richard N Perham
Structure of the capsid of Pf3 filamentous phage determined from X-ray fibre diffraction data at 3.1 A resolution.
Journal of molecular biology 1998;283(1):155-77.
1997: M D Allen; Richard N Perham
The catalytic domain of dihydrolipoyl acetyltransferase from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Expression, purification and reversible denaturation.
FEBS letters 1997;413(2):339-43.
1997: TD Terry; P Malik; Richard N Perham
Accessibility of peptides displayed on filamentous bacteriophage virions: susceptibility to proteinases.
Biological chemistry 1997;378(6):523-30.
1997: R Jelinek; TD Terry; JJ Gesell; P Malik; Richard N Perham; Stanley J Opella
NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
Journal of molecular biology 1997;266(4):649-55.
1997: P Malik; Richard N Perham
Simultaneous display of different peptides on the surface of filamentous bacteriophage.
Nucleic acids research 1997;25(4):915-6.
1996: I A Lessard; C Fuller; Richard N Perham
Competitive interaction of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: kinetic analysis using surface plasmon resonance detection.
Biochemistry 1996;35(51):16863-70.
1996: P M Ricaud; Mark J Howard; E L Roberts; R William Broadhurst; Richard N Perham
Three-dimensional structure of the lipoyl domain from the dihydrolipoyl succinyltransferase component of the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli.
Journal of molecular biology 1996;264(1):179-90.
1996: nicola wallis; M D Allen; R William Broadhurst; I A Lessard; Richard N Perham
Recognition of a surface loop of the lipoyl domain underlies substrate channelling in the pyruvate dehydrogenase multienzyme complex.
Journal of molecular biology 1996;263(3):463-74.
1996: P Malik; TD Terry; L R Gowda; A Langara; S A Petukhov; Martyn F Symmons; LC Welsh; DA Marvin; Richard N Perham
Role of capsid structure and membrane protein processing in determining the size and copy number of peptides displayed on the major coat protein of filamentous bacteriophage.
Journal of molecular biology 1996;260(1):9-21.
1996: P Malik; Richard N Perham
New vectors for peptide display on the surface of filamentous bacteriophage.
Gene 1996;171(1):49-51.
1996: A Bashir; L D Arscott; Richard N Perham; C H Williams; A Berry
The oxidative and reductive half reactions of subunit interface mutants of Escherichia coli glutathione reductase.
Biochemical Society transactions 1996;24(1):9S.
1996: Richard N Perham; B Leistler; R G Solomon; Purnananda Guptasarma
Protein engineering of domains in flavoprotein disulphide oxidoreductases: contributions to folding and assembly.
Biochemical Society transactions 1996;24(1):61-6.
1995: A Bashir; Richard N Perham; Nigel S Scrutton; A Berry
Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase.
The Biochemical journal 1995;312 ( Pt 2)():527-33.
1995: Richard N Perham; TD Terry; Anne E Willis; J Greenwood; F di Marzo Veronese; E Appella
Engineering a peptide epitope display system on filamentous bacteriophage.
FEMS microbiology reviews 1995;17(1-2):25-31.
1995: J D Green; Ernest D Laue; Richard N Perham; S T Ali; John R Guest
Three-dimensional structure of a lipoyl domain from the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
Journal of molecular biology 1995;248(2):328-43.
1995: I A Lessard; Richard N Perham
Interaction of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: stoichiometry and specificity in self-assembly.
The Biochemical journal 1995;306 ( Pt 3)():727-33.
1995: Martyn F Symmons; LC Welsh; C Nave; DA Marvin; Richard N Perham
Matching electrostatic charge between DNA and coat protein in filamentous bacteriophage. Fibre diffraction of charge-deletion mutants.
Journal of molecular biology 1995;245(2):86-91.
1995: Richard N Perham
Structure and posttranslational modification of lipoyl domain of 2-oxo-acid dehydrogenase multienzyme complexes.
Methods in enzymology 1995;251():436-48.
1994: P Rietveld; L David Arscott; A Berry; Nigel S Scrutton; M P Deonarain; Richard N Perham; Charles H Williams
Reductive and oxidative half-reactions of glutathione reductase from Escherichia coli.
Biochemistry 1994;33(46):13888-95.
1994: F di Marzo Veronese; Anne E Willis; C Boyer-Thompson; E Appella; Richard N Perham
Structural mimicry and enhanced immunogenicity of peptide epitopes displayed on filamentous bacteriophage. The V3 loop of HIV-1 gp120.
Journal of molecular biology 1994;243(2):167-72.
1994: S M Brocklehurst; Y N Kalia; Richard N Perham
Protein-protein recognition mediated by a mini-protein domain: possible evolutionary significance.
Trends in biochemical sciences 1994;19(9):360-1.
1994: Maria Rescigno; Richard N Perham
Structure of the NADPH-binding motif of glutathione reductase: efficiency determined by evolution.
Biochemistry 1994;33(19):5721-7.
1994: I A Lessard; Richard N Perham
Expression in Escherichia coli of genes encoding the E1 alpha and E1 beta subunits of the pyruvate dehydrogenase complex of Bacillus stearothermophilus and assembly of a functional E1 component (alpha 2 beta 2) in vitro.
The Journal of biological chemistry 1994;269(14):10378-83.
1994: B Leistler; Richard N Perham
Solubilizing buried domains of proteins: a self-assembling interface domain from glutathione reductase.
Biochemistry 1994;33(10):2773-81.
1994: nicola wallis; Richard N Perham
Structural dependence of post-translational modification and reductive acetylation of the lipoyl domain of the pyruvate dehydrogenase multienzyme complex.
Journal of molecular biology 1994;236(1):209-16.
1994: D S Hipps; Len C Packman; M D Allen; C Fuller; K Sakaguchi; E Appella; Richard N Perham
The peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase complex of Bacillus stearothermophilus: preparation and characterization of its binding to the dihydrolipoyl dehydrogenase component.
The Biochemical journal 1994;297 ( Pt 1)():137-43.
1993: Anne E Willis; Richard N Perham; D Wraith
Immunological properties of foreign peptides in multiple display on a filamentous bacteriophage.
Gene 1993;128(1):79-83.
1993: S M Brocklehurst; Richard N Perham
Prediction of the three-dimensional structures of the biotinylated domain from yeast pyruvate carboxylase and of the lipoylated H-protein from the pea leaf glycine cleavage system: a new automated method for the prediction of protein tertiary structure.
Protein science : a publication of the Protein Society 1993;2(4):626-39.
1993: J A Bocanegra; Nigel S Scrutton; Richard N Perham
Creation of an NADP-dependent pyruvate dehydrogenase multienzyme complex by protein engineering.
Biochemistry 1993;32(11):2737-40.
1993: Y N Kalia; S M Brocklehurst; D S Hipps; E Appella; K Sakaguchi; Richard N Perham
The high-resolution structure of the peripheral subunit-binding domain of dihydrolipoamide acetyltransferase from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
Journal of molecular biology 1993;230(1):323-41.
1993: Frédéric Dardel; A L Davis; Ernest D Laue; Richard N Perham
Three-dimensional structure of the lipoyl domain from Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex.
Journal of molecular biology 1993;229(4):1037-48.
1992: J D Green; Richard N Perham; Stephen Ullrich; E Appella
Conformational studies of the interdomain linker peptides in the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
The Journal of biological chemistry 1992;267(33):23484-8.
1992: Nigel S Scrutton; M P Deonarain; A Berry; Richard N Perham
Cooperativity induced by a single mutation at the subunit interface of a dimeric enzyme: glutathione reductase.
Science (New York, N.Y.) 1992;258(5085):1140-3.
1992: A D Molina-Garcia; Stephen E Harding; F G Diaz; J G de la Torre; D Rowitch; Richard N Perham
Effect of coat protein mutations in bacteriophage fd studied by sedimentation analysis.
Biophysical journal 1992;63(5):1293-8.
1992: G Hale; nicola wallis; Richard N Perham
Interaction of avidin with the lipoyl domains in the pyruvate dehydrogenase multienzyme complex: three-dimensional location and similarity to biotinyl domains in carboxylases.
Proceedings. Biological sciences / The Royal Society 1992;248(1323):247-53.
1992: D S Hipps; Richard N Perham
Expression in Escherichia coli of a sub-gene encoding the lipoyl and peripheral subunit-binding domains of the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex of Bacillus stearothermophilus.
The Biochemical journal 1992;283 ( Pt 3)():665-71.
1992: M P Deonarain; Nigel S Scrutton; Richard N Perham
Engineering surface charge. 2. A method for purifying heterodimers of Escherichia coli glutathione reductase.
Biochemistry 1992;31(5):1498-504.
1992: M P Deonarain; Nigel S Scrutton; Richard N Perham
Engineering surface charge. 1. A method for detecting subunit exchange in Escherichia coli glutathione reductase.
Biochemistry 1992;31(5):1491-7.
1992: Richard N Perham; A Borges; Frédéric Dardel; Lloyd Graham; C F Hawkins; Ernest D Laue; Len C Packman
The lipoyl domain and its role in thiamin diphosphate-dependent oxidative decarboxylation.
Journal of nutritional science and vitaminology 1992;Spec No():457-60.
1991: Frédéric Dardel; Ernest D Laue; Richard N Perham
Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex.
European journal of biochemistry / FEBS 1991;201(1):203-9.
1991: Richard N Perham; Nigel S Scrutton; A Berry
New enzymes for old: redesigning the coenzyme and substrate specificities of glutathione reductase.
BioEssays : news and reviews in molecular, cellular and developmental biology 1991;13(10):515-25.
1991: Richard N Perham
Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein.
Biochemistry 1991;30(35):8501-12.
1991: J Greenwood; Anne E Willis; Richard N Perham
Multiple display of foreign peptides on a filamentous bacteriophage. Peptides from Plasmodium falciparum circumsporozoite protein as antigens.
Journal of molecular biology 1991;220(4):821-7.
1991: Andrew G Allen; Richard N Perham
Two lipoyl domains in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase multienzyme complex of Streptococcus faecalis.
FEBS letters 1991;287(1-2):206-10.
1991: Len C Packman; B Green; Richard N Perham
Lipoylation of the E2 components of the 2-oxo acid dehydrogenase multienzyme complexes of Escherichia coli.
The Biochemical journal 1991;277 ( Pt 1)():153-8.
1991: J Greenwood; G J Hunter; Richard N Perham
Regulation of filamentous bacteriophage length by modification of electrostatic interactions between coat protein and DNA.
Journal of molecular biology 1991;217(2):223-7.
1991: H B Dixon; R A Giddens; R A Harrison; C E Henderson; W E Norris; D M Parker; Richard N Perham; P Slater; M J Sparkes
A synthesis of acylphosphonic acids and of 1-aminoalkylphosphonic acids: the action of pyruvate dehydrogenase and lactate dehydrogenase on acetylphosphonic acid.
Journal of enzyme inhibition 1991;5(2):111-7.
1990: Nigel S Scrutton; A Berry; M P Deonarain; Richard N Perham
Active site complementation in engineered heterodimers of Escherichia coli glutathione reductase created in vivo.
Proceedings. Biological sciences / The Royal Society 1990;242(1305):217-24.
1990: A Borges; C F Hawkins; Len C Packman; Richard N Perham
Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
European journal of biochemistry / FEBS 1990;194(1):95-102.
1990: M P Deonarain; Nigel S Scrutton; A Berry; Richard N Perham
Directed mutagenesis of the redox-active disulphide bridge in glutathione reductase from Escherichia coli.
Proceedings. Biological sciences / The Royal Society 1990;241(1302):179-86.
1990: C F Hawkins; A Borges; Richard N Perham
Cloning and sequence analysis of the genes encoding the alpha and beta subunits of the E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
European journal of biochemistry / FEBS 1990;191(2):337-46.
1990: Frédéric Dardel; Len C Packman; Richard N Perham
Expression in Escherichia coli of a sub-gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of Bacillus stearothermophilus.
FEBS letters 1990;264(2):206-10.
1990: Richard N Perham
The fructose-1,6-bisphosphate aldolases: same reaction, different enzymes.
Biochemical Society transactions 1990;18(2):185-7.
1990: Lloyd Graham; Richard N Perham
Interactions of lipoyl domains with the E1p subunits of the pyruvate dehydrogenase multienzyme complex from Escherichia coli.
FEBS letters 1990;262(2):241-4.
1990: Nigel S Scrutton; A Berry; Richard N Perham
Redesign of the coenzyme specificity of a dehydrogenase by protein engineering.
Nature 1990;343(6253):38-43.
1989: M P Deonarain; A Berry; Nigel S Scrutton; Richard N Perham
Alternative proton donors/acceptors in the catalytic mechanism of the glutathione reductase of Escherichia coli: the role of histidine-439 and tyrosine-99.
Biochemistry 1989;28(25):9602-7.
1989: C F Hawkins; A Borges; Richard N Perham
A common structural motif in thiamin pyrophosphate-binding enzymes.
FEBS letters 1989;255(1):77-82.
1989: Andrew G Allen; Richard N Perham; N Allison; J S Miles; John R Guest
Reductive acetylation of tandemly repeated lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli is random order.
Journal of molecular biology 1989;208(4):623-33.
1989: J Greenwood; Richard N Perham
Dual importance of positive charge in the C-terminal region of filamentous bacteriophage coat protein for membrane insertion and DNA-protein interaction in virus assembly.
Virology 1989;171(2):444-52.
1989: S E Radford; Richard N Perham; Stephen Ullrich; E Appella
Antibodies against an inter-domain segment of polypeptide chain inhibit active-site coupling in the pyruvate dehydrogenase multienzyme complex.
FEBS letters 1989;250(2):336-40.
1989: P R Alefounder; Richard N Perham
Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.
Molecular microbiology 1989;3(6):723-32.
1989: Lloyd Graham; Len C Packman; Richard N Perham
Kinetics and specificity of reductive acylation of lipoyl domains from 2-oxo acid dehydrogenase multienzyme complexes.
Biochemistry 1989;28(4):1574-81.
1989: A Berry; Nigel S Scrutton; Richard N Perham
Switching kinetic mechanism and putative proton donor by directed mutagenesis of glutathione reductase.
Biochemistry 1989;28(3):1264-9.
1989: P R Alefounder; S A Baldwin; Richard N Perham; N J Short
Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.
The Biochemical journal 1989;257(2):529-34.
1989: S E Radford; Ernest D Laue; Richard N Perham; S R Martin; E Appella
Conformational flexibility and folding of synthetic peptides representing an interdomain segment of polypeptide chain in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
The Journal of biological chemistry 1989;264(2):767-75.
1989: Richard N Perham; Len C Packman
2-Oxo acid dehydrogenase multienzyme complexes: domains, dynamics, and design.
Annals of the New York Academy of Sciences 1989;573():1-20.
1988: D H Rowitch; G J Hunter; Richard N Perham
Variable electrostatic interaction between DNA and coat protein in filamentous bacteriophage assembly.
Journal of molecular biology 1988;204(3):663-74.
1988: Nigel S Scrutton; A Berry; Richard N Perham
Engineering of an intersubunit disulphide bridge in glutathione reductase from Escherichia coli.
FEBS letters 1988;241(1-2):46-50.
1988: J S Miles; John R Guest; S E Radford; Richard N Perham
Investigation of the mechanism of active site coupling in the pyruvate dehydrogenase multienzyme complex of Escherichia coli by protein engineering.
Journal of molecular biology 1988;202(1):97-106.
1988: Len C Packman; A Borges; Richard N Perham
Amino acid sequence analysis of the lipoyl and peripheral subunit-binding domains in the lipoate acetyltransferase component of the pyruvate dehydrogenase complex from Bacillus stearothermophilus.
The Biochemical journal 1988;252(1):79-86.
1988: Richard N Perham; A Berry; Nigel S Scrutton
Flavoprotein disulphide oxidoreductases: protein engineering of glutathione reductase from Escherichia coli.
Biochemical Society transactions 1988;16(2):84-7.
1988: F L Texter; S E Radford; Ernest D Laue; Richard N Perham; J S Miles; John R Guest
Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
Biochemistry 1988;27(1):289-96.
1987: S E Radford; Ernest D Laue; Richard N Perham; J S Miles; John R Guest
Segmental structure and protein domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. Genetic reconstruction in vitro and 1H-n.m.r. spectroscopy.
The Biochemical journal 1987;247(3):641-9.
1987: Richard N Perham
Glutathione reductase from Escherichia coli: mutation, cloning and sequence analysis of the gene.
Biochemical Society transactions 1987;15(4):730-3.
1987: Nigel S Scrutton; A Berry; Richard N Perham
Purification and characterization of glutathione reductase encoded by a cloned and over-expressed gene in Escherichia coli.
The Biochemical journal 1987;245(3):875-80.
1987: D H Rowitch; Richard N Perham
Cloning and expression of the filamentous bacteriophage Pf1 major coat protein gene in Escherichia coli. Membrane protein processing and virus assembly.
Journal of molecular biology 1987;195(4):873-84.
1987: J S Miles; John R Guest; S E Radford; Richard N Perham
A mutant pyruvate dehydrogenase complex of Escherichia coli deleted in the (alanine + proline)-rich region of the acetyltransferase component.
Biochimica et biophysica acta 1987;913(2):117-21.
1987: G J Hunter; D H Rowitch; Richard N Perham
Interactions between DNA and coat protein in the structure and assembly of filamentous bacteriophage fd.
Nature 1987;327(6119):252-4.
1987: Richard N Perham; Len C Packman; S E Radford
2-Oxo acid dehydrogenase multi-enzyme complexes: in the beginning and halfway there.
Biochemical Society symposium 1987;54():67-81.
1985: J R Guest; H M Lewis; Lloyd Graham; Len C Packman; Richard N Perham
Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
Journal of molecular biology 1985;185(4):743-54.
1981: C Nave; R S Brown; A G Fowler; Jane E Ladner; DA Marvin; S W Provencher; A Tsugita; J Armstrong; Richard N Perham
Pf1 filamentous bacterial virus. X-ray fibre diffraction analysis of two heavy-atom derivatives.
Journal of molecular biology 1981;149(4):675-707.
1970: P J Anderson; Richard N Perham
The thiol groups of aldolase.
The Biochemical journal 1970;117(3):49P-50P.
1967: Richard N Perham
A diagonal paper-electrophoretic technique for studying amino acid sequences around the cysteine and cystine residues of proteins.
The Biochemical journal 1967;105(3):1203-7.
1966: Richard N Perham
An extended amino acid sequence around the reactive cysteine residues of glyceraldehyde 3-phosphate dehydrogenase.
The Biochemical journal 1966;99(2):14C-6C.