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Frank Bernhard

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Publications

TOP 3
Karsten Mörs; Christian Roos; Frank Scholz; Josef Wachtveitl; Frank Bernhard; Volker Dötsch; Clemens Glaubitz
Modified lipid and protein dynamics in nanodiscs.
Christian Roos; Volker Dötsch; Slawomir Filipek; Umesh Ghoshdastider; Lei Kai; Davide Proverbio; Frank Bernhard
Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.
Lei Kai; Ralf Kaldenhoff; Volker Dötsch; Frank Bernhard
Artificial environments for the co-translational stabilization of cell-free expressed proteins.

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All Publications

2013: Karsten Mörs; Christian Roos; Frank Scholz; Josef Wachtveitl; Frank Bernhard; Volker Dötsch; Clemens Glaubitz
Modified lipid and protein dynamics in nanodiscs.
Biochimica et biophysica acta 2013;1828(4):1222-9.
2013: Christian Roos; Volker Dötsch; Slawomir Filipek; Umesh Ghoshdastider; Lei Kai; Davide Proverbio; Frank Bernhard
Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.
Molecular membrane biology 2013;30(1):75-89.
2013: Lei Kai; Ralf Kaldenhoff; Volker Dötsch; Frank Bernhard
Artificial environments for the co-translational stabilization of cell-free expressed proteins.
PloS one 2013;8(2):e56637.
2012: Yi Ma; Jufang Wang; Wei Ye; Frank Bernhard; Volker Dötsch; Slawomir Filipek; Umesh Ghoshdastider; Xiaoning Wang
Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening.
Protein expression and purification 2012;86(2):120-6.
2012: Christian Roos; Hans-Georg Sahl; Tanja Schneider; Frank Scholz; Josef Wachtveitl; Michael Zocher; Volker Dötsch; Erik Henrich; Yi Ma; Daniel Müller; Daniela Münch; Davide Proverbio; Frank Bernhard
Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.
Biochimica et biophysica acta 2012;1818(12):3098-106.
2012: Stefan Haberstock; Yvette Hoevels; Alexander Pautsch; Christian Roos; Gisela Schnapp; Volker Dötsch; Frank Bernhard
A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.
Protein expression and purification 2012;82(2):308-16.
2012: Julia von Bülow; Frank Bernhard; Lei Kai; Annika Müller-Lucks; Eric Beitz
Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism.
The Journal of biological chemistry 2012;287(10):7487-94.
2012: Frank Löhr; Jonathan M Moore; Sina Reckel; Mikhail S. Karbyshev; Norzehan Abdul-Manan; Frank Bernhard; Peter J Connolly; Volker Dötsch
Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment.
Journal of biomolecular NMR 2012;52(3):197-210.
2012: Lei Kai; Mikhail S. Karbyshev; Yi Ma; Davide Proverbio; Christian Roos; Volker Dötsch; Stefan Haberstock; Friederike Junge; Frank Bernhard
Systems for the cell-free synthesis of proteins.
Methods in molecular biology (Clifton, N.J.) 2012;800():201-25.
2011: Sina Reckel; Harald Schwalbe; Robert Silvers; Jochen Stehle; Mitsuhiro Takeda; Mirka-Kristin Verhoefen; Josef Wachtveitl; Clemens Glaubitz; Daniel Gottstein; Peter Güntert; Masatsune Kainosho; Frank Löhr; Frank Bernhard; Volker Dötsch
Solution NMR structure of proteorhodopsin.
Angewandte Chemie (International ed. in English) 2011;50(50):11942-6.
2011: Yi Ma; Daniela Münch; Hans-Georg Sahl; Tanja Schneider; Jufang Wang; Xiaoning Wang; Ahmed Bouhss; Volker Dötsch; Umesh Ghoshdastider; Frank Bernhard
Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.
The Journal of biological chemistry 2011;286(45):38844-53.
2011: Doreen Matthies; Janet Vonck; Frank Bernhard; Volker Dötsch; Stefan Haberstock; Friederike Joos; Thomas Meier
Cell-free expression and assembly of ATP synthase.
Journal of molecular biology 2011;413(3):593-603.
2011: Susanne Stefer; Fei Wang; Klemens Wild; Frank Bernhard; Jörg Bomke; Vladimir Denic; Volker Dötsch; Christopher Hein; Frank Löhr; Yin-Yuin Pang; Simon Reitz; Daniel Schwarz; Irmgard Sinning
Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.
Science (New York, N.Y.) 2011;333(6043):758-62.
2011: Vladimir V Rogov; Natalia Yu Rogova; Frank Bernhard; Frank Löhr; Volker Dötsch
A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF.
The Journal of biological chemistry 2011;286(21):18775-83.
2011: Friederike Junge; Stefan Haberstock; Christian Roos; Susanne Stefer; Davide Proverbio; Volker Dötsch; Frank Bernhard
Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.
New biotechnology 2011;28(3):262-71.
2011: Kerstin Schmöe; Vladimir V Rogov; Natalia Yu Rogova; Frank Löhr; Peter Güntert; Frank Bernhard; Volker Dötsch
Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB.
Structure (London, England : 1993) 2011;19(4):577-87.
2010: Friederike Junge; Laura M Luh; Davide Proverbio; Birgit Schäfer; Rupert Abele; Michael Beyermann; Volker Dötsch; Frank Bernhard
Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor.
Journal of structural biology 2010;172(1):94-106.
2010: Solmaz Sobhanifar; Birgit Schneider; Frank Löhr; Daniel Gottstein; Teppei Ikeya; Krzysztof Mlynarczyk; Wojciech Pulawski; Umesh Ghoshdastider; Michal Kolinski; Slawomir Filipek; Peter Güntert; Frank Bernhard; Volker Dötsch
Structural investigation of the C-terminal catalytic fragment of presenilin 1.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(21):9644-9.
2010: Daniel Schwarz; Daniel Daley; Tobias Beckhaus; Volker Dötsch; Frank Bernhard
Cell-free expression profiling of E. coli inner membrane proteins.
Proteomics 2010;10(9):1762-79.
2010: Sina Reckel; Solmaz Sobhanifar; Florian Durst; Frank Löhr; Vladimir A Shirokov; Volker Dötsch; Frank Bernhard
Strategies for the cell-free expression of membrane proteins.
Methods in molecular biology (Clifton, N.J.) 2010;607():187-212.
2010: Birgit Schneider; Friederike Junge; Vladimir A Shirokov; Florian Durst; Daniel Schwarz; Volker Dötsch; Frank Bernhard
Membrane protein expression in cell-free systems.
Methods in molecular biology (Clifton, N.J.) 2010;601():165-86.
2010: Solmaz Sobhanifar; Sina Reckel; Friederike Junge; Daniel Schwarz; Lei Kai; Mikhail S. Karbyshev; Frank Löhr; Frank Bernhard; Volker Dötsch
Cell-free expression and stable isotope labelling strategies for membrane proteins.
Journal of biomolecular NMR 2010;46(1):33-43.
2009: Alena E L Busche; A Sesilja Aranko; Mehdi Talebzadeh-Farooji; Frank Bernhard; Volker Dötsch; Hideo Iwaï
Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.
Angewandte Chemie (International ed. in English) 2009;48(33):6128-31.
2008: Vladimir V Rogov; Kerstin Schmöe; Frank Löhr; Natalia Yu Rogova; Frank Bernhard; Volker Dötsch
Modulation of the Rcs-mediated signal transfer by conformational flexibility.
Biochemical Society transactions 2008;36(Pt 6):1427-32.
2008: Daniel Schwarz; Volker Dötsch; Frank Bernhard
Production of membrane proteins using cell-free expression systems.
Proteomics 2008;8(19):3933-46.
2008: Alexander Koglin; Frank Löhr; Frank Bernhard; Vladimir V Rogov; Dominique Frueh; Eric R. Strieter; Mohammad R Mofid; Peter Güntert; Gerhard Wagner; Christopher T Walsh; Mohamed A Marahiel; Volker Dötsch
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase.
Nature 2008;454(7206):907-11.
2008: Sina Reckel; Solmaz Sobhanifar; Birgit Schneider; Friederike Junge; Daniel Schwarz; Florian Durst; Frank Löhr; Peter Güntert; Frank Bernhard; Volker Dötsch
Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(24):8262-7.
2008: F Junge; Birgit Schneider; Sina Reckel; Daniel Schwarz; Volker Dötsch; Frank Bernhard
Large-scale production of functional membrane proteins.
Cellular and molecular life sciences : CMLS 2008;65(11):1729-55.
2008: M Weidlich; Christian Klammt; Frank Bernhard; M Karas; T Stein
Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches.
Letters in applied microbiology 2008;46(2):155-9.
2007: Christian Klammt; Daniel Schwarz; Nora Eifler; Andreas Engel; Jacob Piehler; Winfried Haase; Steffen Hahn; Volker Dötsch; Frank Bernhard
Reprint of "Cell-free production of G protein-coupled receptors for functional and structural studies" [J. Struct. Biol. 158 (2007) 482-493].
Journal of structural biology 2007;159(2):194-205.
2007: Christian Klammt; Ankita Srivastava; Nora Eifler; Friederike Junge; Michael Beyermann; Daniel Schwarz; Hartmut Michel; Volker Doetsch; Frank Bernhard
Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation.
The FEBS journal 2007;274(13):3257-69.
2007: Christian Klammt; Daniel Schwarz; Nora Eifler; Andreas Engel; Jacob Piehler; Winfried Haase; Steffen Hahn; Volker Dötsch; Frank Bernhard
Cell-free production of G protein-coupled receptors for functional and structural studies.
Journal of structural biology 2007;158(3):482-93.
2007: Vladimir V Rogov; Frank Löhr; Natalia Rogova; Christian Klammt; Alexander Koglin; Frank Bernhard; Volker Dötsch
NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.
Journal of biomolecular NMR 2007;38(2):165.
2007: Daniel Schwarz; Christian Klammt; Alexander Koglin; Frank Löhr; Birgit Schneider; Volker Dötsch; Frank Bernhard
Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies.
Methods (San Diego, Calif.) 2007;41(4):355-69.
2007: Daniel Schwarz; Friederike Junge; Florian Durst; Nadine Frölich; Birgit Schneider; Sina Reckel; Solmaz Sobhanifar; Volker Dötsch; Frank Bernhard
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.
Nature protocols 2007;2(11):2945-57.
2007: Christian Klammt; Daniel Schwarz; Volker Dötsch; Frank Bernhard
Cell-free production of integral membrane proteins on a preparative scale.
Methods in molecular biology (Clifton, N.J.) 2007;375():57-78.
2006: Vladimir V Rogov; Natalia Yu Rogova; Frank Bernhard; Alexander Koglin; Frank Löhr; Volker Dötsch
A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.
Journal of molecular biology 2006;364(1):68-79.
2006: Christian Klammt; Daniel Schwarz; Frank Löhr; Birgit Schneider; Volker Dötsch; Frank Bernhard
Cell-free expression as an emerging technique for the large scale production of integral membrane protein.
The FEBS journal 2006;273(18):4141-53.
2006: Alexander Koglin; Christian Klammt; Nikola Trbovic; Daniel Schwarz; Birgit Schneider; Birgit Schäfer; Frank Löhr; Frank Bernhard; Volker Dötsch
Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.
Magnetic resonance in chemistry : MRC 2006;44 Spec No():S17-23.
2006: Alexander Koglin; Mohammad R Mofid; Frank Löhr; Birgit Schäfer; Vladimir V Rogov; Marc-Michael Blum; Tanja Mittag; Mohamed A Marahiel; Frank Bernhard; Volker Dötsch
Conformational switches modulate protein interactions in peptide antibiotic synthetases.
Science (New York, N.Y.) 2006;312(5771):273-6.
2005: Christian Klammt; Daniel Schwarz; Klaus Fendler; Winfried Haase; Volker Dötsch; Frank Bernhard
Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system.
The FEBS journal 2005;272(23):6024-38.
2005: Nikola Trbovic; Christian Klammt; Alexander Koglin; Frank Löhr; Frank Bernhard; Volker Dötsch
Efficient strategy for the rapid backbone assignment of membrane proteins.
Journal of the American Chemical Society 2005;127(39):13504-5.
2005: Frank Löhr; Vladimir V Rogov; Meichen Shi; Frank Bernhard; Volker Dötsch
Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues.
Journal of biomolecular NMR 2005;32(4):309-28.
2004: Vladimir V Rogov; Frank Bernhard; Frank Löhr; Volker Dötsch
Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.
Journal of molecular biology 2004;343(4):1035-48.
2004: Vladimir V Rogov; Frank Löhr; Frank Bernhard; Volker Dötsch
Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain.
Journal of biomolecular NMR 2004;30(1):103-4.
2004: Christian Klammt; Frank Löhr; Birgit Schäfer; Winfried Haase; Volker Dötsch; Heinz Rüterjans; Clemens Glaubitz; Frank Bernhard
High level cell-free expression and specific labeling of integral membrane proteins.
European journal of biochemistry / FEBS 2004;271(3):568-80.
2003: Primoz Pristovsek; Kaushik Sengupta; Frank Löhr; Birgit Schäfer; Markus Wehland von Trebra; Heinz Rüterjans; Frank Bernhard
Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box.
The Journal of biological chemistry 2003;278(20):17752-9.