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Rita Bernhardt

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Simon Janocha; Michael Kleser; Josef Zapp; Jörg Bohlmann; Michael Hutter; Rita Bernhardt
Resin acid conversion with CYP105A1: an enzyme with potential for the production of pharmaceutically relevant diterpenoids.
Yogan Khatri; Emmanuelle Leize-Wagner; Aurélie Même; Michael Ringle; Vlada B Urlacher; Marco Girhard; Frank Hannemann; Simon Janocha; Reinhard Kappl; Rita Bernhardt
Novel family members of CYP109 from Sorangium cellulosum So ce56 exhibit characteristic biochemical and biophysical properties.
Anna Hobler; Michael C Hutter; Norio Kagawa; Stefan A Wudy; Frank Hannemann; Michaela F Hartmann; Rita Bernhardt
Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level.

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2013: Simon Janocha; Michael Kleser; Josef Zapp; Jörg Bohlmann; Michael Hutter; Rita Bernhardt
Resin acid conversion with CYP105A1: an enzyme with potential for the production of pharmaceutically relevant diterpenoids.
Chembiochem : a European journal of chemical biology 2013;14(4):467-73.
2013: Yogan Khatri; Emmanuelle Leize-Wagner; Aurélie Même; Michael Ringle; Vlada B Urlacher; Marco Girhard; Frank Hannemann; Simon Janocha; Reinhard Kappl; Rita Bernhardt
Novel family members of CYP109 from Sorangium cellulosum So ce56 exhibit characteristic biochemical and biophysical properties.
Biotechnology and applied biochemistry 2013;60(1):18-29.
2012: Anna Hobler; Michael C Hutter; Norio Kagawa; Stefan A Wudy; Frank Hannemann; Michaela F Hartmann; Rita Bernhardt
Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level.
The Journal of steroid biochemistry and molecular biology 2012;132(1-2):57-65.
2012: Thuy T B Ly; Josef Zapp; Michael C Hutter; Yogan Khatri; Rita Bernhardt
CYP264B1 from Sorangium cellulosum So ce56: a fascinating norisoprenoid and sesquiterpene hydroxylase.
Applied microbiology and biotechnology 2012;95(1):123-33.
2012: Kerstin Maria Ewen; Michael Ringle; Rita Bernhardt
Adrenodoxin--a versatile ferredoxin.
IUBMB life 2012;64(6):506-12.
2012: Kim Thoa Nguyen; Cornelia Virus; Nils Günnewich; Frank Hannemann; Rita Bernhardt
Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone.
Chembiochem : a European journal of chemical biology 2012;13(8):1161-6.
2012: Daniela Schmitz; Josef Zapp; Rita Bernhardt
Hydroxylation of the triterpenoid dipterocarpol with CYP106A2 from Bacillus megaterium.
The FEBS journal 2012;279(9):1663-74.
2012: Antje Eiden-Plach; Frank Hannemann; Michaela F Hartmann; Huy-Hoang Nguyen; Ursula Schneider; Rita Bernhardt; Stefan A Wudy
Alu Sx repeat-induced homozygous deletion of the StAR gene causes lipoid congenital adrenal hyperplasia.
The Journal of steroid biochemistry and molecular biology 2012;130(1-2):1-6.
2012: Martin Wörner; Christian G. Huber; Katja Melchior; Katalin Monostory; Jean-Marc Pascussi; Rita Bernhardt
The effects of rosuvastatin and the CYP51A1 inhibitor LEK-935 on the proteome of primary human hepatocytes.
Drug metabolism and disposition: the biological fate of chemicals 2012;40(3):414-8.
2012: Michael Kleser; Josef Zapp; Frank Hannemann; Michael Hutter; Rita Bernhardt
CYP105A1 mediated 3-hydroxylation of glimepiride and glibenclamide using a recombinant Bacillus megaterium whole-cell catalyst.
Journal of biotechnology 2012;157(3):405-12.
2012: Sabrina Bleif; Frank Hannemann; David Hartmann; Johann Jauch; Josef Zapp; Rita Bernhardt
A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-β-boswellic acid (KBA) based on a recombinant cytochrome P450 system.
Applied microbiology and biotechnology 2012;93(3):1135-46.
2011: Burkhard Schiffler; Andy Zöllner; Rita Bernhardt
Kinetic and optical biosensor study of adrenodoxin mutant AdxS112W displaying an enhanced interaction towards the cholesterol side chain cleavage enzyme (CYP11A1).
European biophysics journal : EBJ 2011;40(12):1275-82.
2011: Kerstin Maria Ewen; Frank Hannemann; Stefania Iametti; Anna Morleo; Rita Bernhardt
Functional characterization of Fdx1: evidence for an evolutionary relationship between P450-type and ISC-type ferredoxins.
Journal of molecular biology 2011;413(5):940-51.
2011: Jürgen J Müller; Frank Hannemann; Burkhard Schiffler; Kerstin M Ewen; Reinhard Kappl; Udo Heinemann; Rita Bernhardt
Structural and thermodynamic characterization of the adrenodoxin-like domain of the electron-transfer protein Etp1 from Schizosaccharomyces pombe.
Journal of inorganic biochemistry 2011;105(7):957-65.
2011: Yogan Khatri; Frank Hannemann; Olena Perlova; Rolf Müller; Rita Bernhardt
Investigation of cytochromes P450 in myxobacteria: excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56.
FEBS letters 2011;585(11):1506-13.
2011: Tarek Hakki; Katja Hübel; Herbert Waldmann; Rita Bernhardt
The development of a whole-cell based medium throughput screening system for the discovery of human aldosterone synthase (CYP11B2) inhibitors: old drugs disclose new applications for the therapy of congestive heart failure, myocardial fibrosis and hypertension.
The Journal of steroid biochemistry and molecular biology 2011;125(1-2):120-8.
2011: Ming Kwai Tin; Tarek Hakki; Rita Bernhardt
Fission yeast Schizosaccharomyces pombe as a new system for the investigation of corticosterone methyloxidase deficiency-causing mutations.
The Journal of steroid biochemistry and molecular biology 2011;124(1-2):31-7.
2011: Simon Janocha; Andreas Bichet; Andy Zöllner; Rita Bernhardt
Substitution of lysine with glutamic acid at position 193 in bovine CYP11A1 significantly affects protein oligomerization and solubility but not enzymatic activity.
Biochimica et biophysica acta 2011;1814(1):126-31.
2011: Kerstin Maria Ewen; Michael Kleser; Rita Bernhardt
Adrenodoxin: the archetype of vertebrate-type [2Fe-2S] cluster ferredoxins.
Biochimica et biophysica acta 2011;1814(1):111-25.
2010: Yogan Khatri; Frank Hannemann; Kerstin M Ewen; Dominik Pistorius; Olena Perlova; Norio Kagawa; Alexander O Brachmann; Rolf Müller; Rita Bernhardt
The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase.
Chemistry & biology 2010;17(12):1295-305.
2010: Yogan Khatri; Marco Girhard; Anna Romankiewicz; Michael Ringle; Frank Hannemann; Vlada B Urlacher; Michael C Hutter; Rita Bernhardt
Regioselective hydroxylation of norisoprenoids by CYP109D1 from Sorangium cellulosum So ce56.
Applied microbiology and biotechnology 2010;88(2):485-95.
2010: Huy-Hoang Nguyen; Frank Hannemann; Michaela F Hartmann; Ewa M Malunowicz; Stefan A Wudy; Rita Bernhardt
Five novel mutations in CYP11B2 gene detected in patients with aldosterone synthase deficiency type I: Functional characterization and structural analyses.
Molecular genetics and metabolism 2010;100(4):357-64.
2010: Anja Berwanger; Susanne Eyrisch; inge schuster; Volkhard Helms; Rita Bernhardt
Polyamines: naturally occurring small molecule modulators of electrostatic protein-protein interactions.
Journal of inorganic biochemistry 2010;104(2):118-25.
2009: Guillermina Goñi; Andy Zöllner; Michael Lisurek; Adrián Velázquez-Campoy; Sira Pinto; Carlos Gómez-Moreno; Frank Hannemann; Rita Bernhardt; Milagros Medina
Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.
Biochimica et biophysica acta 2009;1794(11):1635-42.
2009: Frank Hannemann; Arnaud Guyot; Andy Zöllner; Jürgen J Müller; Udo Heinemann; Rita Bernhardt
The dipole moment of the electron carrier adrenodoxin is not critical for redox partner interaction and electron transfer.
Journal of inorganic biochemistry 2009;103(7):997-1004.
2009: Martin Wörner; Katja Melchior; Nathanael Delmotte; Kyung Hoon Hwang; Katalin Monostory; Christian G. Huber; Rita Bernhardt
Shotgun proteomic analysis of the microsomal fraction of eukaryotic cells using a two-dimensional reversed-phase x ion-pair reversed-phase HPLC setup.
Journal of separation science 2009;32(8):1165-74.
2008: Cornelia Virus; Rita Bernhardt
Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening.
Lipids 2008;43(12):1133-41.
2008: Susanne Zöllner; Kyung Hoon Hwang; Britta Wilzewski; Christine Carapito; Emmanuelle Leize-Wagner; Alain Van Dorsselaer; Rita Bernhardt
Aldosterone: from biosynthesis to non-genomic action onto the proteome.
Steroids 2008;73(9-10):966-72.
2008: Michael Lisurek; Birgit Simgen; Iris Antes; Rita Bernhardt
Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation.
Chembiochem : a European journal of chemical biology 2008;9(9):1439-49.
2008: Kerstin M Ewen; Burkhard Schiffler; Heike Uhlmann-Schiffler; Rita Bernhardt; Frank Hannemann
The endogenous adrenodoxin reductase-like flavoprotein arh1 supports heterologous cytochrome P450-dependent substrate conversions in Schizosaccharomyces pombe.
FEMS yeast research 2008;8(3):432-41.
2008: Huy-Hoang Nguyen; Frank Hannemann; Michaela F Hartmann; Stefan A Wudy; Rita Bernhardt
Aldosterone synthase deficiency caused by a homozygous L451F mutation in the CYP11B2 gene.
Molecular genetics and metabolism 2008;93(4):458-67.
2008: Andy Zöllner; Norio Kagawa; Michael R Waterman; Yasuki Nonaka; Koji Takio; Yoshitsugu Shiro; Frank Hannemann; Rita Bernhardt
Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli.
The FEBS journal 2008;275(4):799-810.
2008: Tarek Hakki; Silvia Zearo; Călin-Aurel Drăgan; Matthias Bureik; Rita Bernhardt
Coexpression of redox partners increases the hydrocortisone (cortisol) production efficiency in CYP11B1 expressing fission yeast Schizosaccharomyces pombe.
Journal of biotechnology 2008;133(3):351-9.
2008: Lyubomira Ivanova; Rita Bernhardt; Ingolf Bernhardt
Nongenomic effect of aldosterone on ion transport pathways of red blood cells.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2008;22(1-4):269-78.
2007: Frank Hannemann; Rita Bernhardt; Joachim Jose
Biocatalytic synthesis of 4-pregnen-20,21-diol-3-one, a selective inhibitor of human 5alpha-reductase type II.
Journal of enzyme inhibition and medicinal chemistry 2007;22(5):570-6.
2007: Andy Zöllner; Melissa A Pasquinelli; Rita Bernhardt; David N Beratan
Protein phosphorylation and intermolecular electron transfer: a joint experimental and computational study of a hormone biosynthesis pathway.
Journal of the American Chemical Society 2007;129(14):4206-16.
2007: Andreas Bichet; Frank Hannemann; Matthias Rekowski; Rita Bernhardt
A new application of the yeast two-hybrid system in protein engineering.
Protein engineering, design & selection : PEDS 2007;20(3):117-23.
2007: Frank Hannemann; Andreas Bichet; Kerstin M Ewen; Rita Bernhardt
Cytochrome P450 systems--biological variations of electron transport chains.
Biochimica et biophysica acta 2007;1770(3):330-44.
2007: Joachim Behlke; Otto Ristau; Eva-Christina Müller; Frank Hannemann; Rita Bernhardt
Self-association of adrenodoxin studied by using analytical ultracentrifugation.
Biophysical chemistry 2007;125(1):159-65.
2006: Cornelia Virus; Michael Lisurek; B Simgen; Frank Hannemann; Rita Bernhardt
Function and engineering of the 15beta-hydroxylase CYP106A2.
Biochemical Society transactions 2006;34(Pt 6):1215-8.
2006: Susanne Böhmer; Christine Carapito; Britta Wilzewski; Emmanuelle Leize; Alain Van Dorsselaer; Rita Bernhardt
Analysis of aldosterone-induced differential receptor-independent protein patterns using 2D-electrophoresis and mass spectrometry.
Biological chemistry 2006;387(7):917-29.
2006: Kyung-Hoon Hwang; Christine Carapito; Susanne Böhmer; Emmanuelle Leize; Alain Van Dorsselaer; Rita Bernhardt
Proteome analysis of Schizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry.
Proteomics 2006;6(14):4115-29.
2006: Tarek Hakki; Rita Bernhardt
CYP17- and CYP11B-dependent steroid hydroxylases as drug development targets.
Pharmacology & therapeutics 2006;111(1):27-52.
2006: Rita Bernhardt
Cytochromes P450 as versatile biocatalysts.
Journal of biotechnology 2006;124(1):128-45.
2006: Frank Hannemann; Cornelia Virus; Rita Bernhardt
Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases.
Journal of biotechnology 2006;124(1):172-81.
2006: Andrei Halavaty; Jürgen J Müller; Jörg Contzen; Christiane Jung; Frank Hannemann; Rita Bernhardt; Marcus Galander; Friedhelm Lendzian; Udo Heinemann
Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure.
Biochemistry 2006;45(3):709-18.
2005: Achim Heinz; Frank Hannemann; Jürgen J Müller; Udo Heinemann; Rita Bernhardt
The interaction domain of the redox protein adrenodoxin is mandatory for binding of the electron acceptor CYP11A1, but is not required for binding of the electron donor adrenodoxin reductase.
Biochemical and biophysical research communications 2005;338(1):491-8.
2005: Britta Diesel; Jens Radermacher; Matthias Bureik; Rita Bernhardt; Markus Seifert; Jörg Reichrath; Ulrike Fischer; Eckart Meese
Vitamin D(3) metabolism in human glioblastoma multiforme: functionality of CYP27B1 splice variants, metabolism of calcidiol, and effect of calcitriol.
Clinical cancer research : an official journal of the American Association for Cancer Research 2005;11(15):5370-80.
2005: Matthias Bureik; Alexander Mion; Christopher J Kenyon; Rita Bernhardt
Inhibition of aldosterone biosynthesis by staurosporine.
Biological chemistry 2005;386(7):663-9.
2005: Matthias Bureik; Nicole Brück; Katja Hübel; Rita Bernhardt
The human mineralocorticoid receptor only partially differentiates between different ligands after expression in fission yeast.
FEMS yeast research 2005;5(6-7):627-33.
2005: Călin-Aurel Drăgan; Silvia Zearo; Frank Hannemann; Rita Bernhardt; Matthias Bureik
Efficient conversion of 11-deoxycortisol to cortisol (hydrocortisone) by recombinant fission yeast Schizosaccharomyces pombe.
FEMS yeast research 2005;5(6-7):621-5.
2005: Sarah Ulmschneider; Ursula Müller-Vieira; Markus Mitrenga; Rolf W Hartmann; Sandrine Oberwinkler-Marchais; Christian D Klein; Matthias Bureik; Rita Bernhardt; Iris Antes; Thomas Lengauer
Synthesis and evaluation of imidazolylmethylenetetrahydronaphthalenes and imidazolylmethyleneindanes: potent inhibitors of aldosterone synthase.
Journal of medicinal chemistry 2005;48(6):1796-805.
2005: Matthias Bureik; Andy Zöllner; Norbert Schuster; Mathias Montenarh; Rita Bernhardt
Phosphorylation of bovine adrenodoxin by protein kinase CK2 affects the interaction with its redox partner cytochrome P450scc (CYP11A1).
Biochemistry 2005;44(10):3821-30.
2004: Burkhard Schiffler; Andy Zöllner; Rita Bernhardt
Stripping down the mitochondrial cholesterol hydroxylase system, a kinetics study.
The Journal of biological chemistry 2004;279(33):34269-76.
2004: Burkhard Schiffler; Matthias Bureik; Wolfgang Reinle; Eva-Christina Müller; Frank Hannemann; Rita Bernhardt
The adrenodoxin-like ferredoxin of Schizosaccharomyces pombe mitochondria.
Journal of inorganic biochemistry 2004;98(7):1229-37.
2004: Michael Lisurek; Min-Jung Kang; Rolf W Hartmann; Rita Bernhardt
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry.
Biochemical and biophysical research communications 2004;319(2):677-82.
2004: Andy Zöllner; Isabel Nogués; Achim Heinz; Milagros Medina; Carlos Gómez-Moreno; Rita Bernhardt
Analysis of the interaction of a hybrid system consisting of bovine adrenodoxin reductase and flavodoxin from the cyanobacterium Anabaena PCC 7119.
Bioelectrochemistry (Amsterdam, Netherlands) 2004;63(1-2):61-5.
2004: Andreas Bichet; Matthias Bureik; Natalie Lenz; Rita Bernhardt
The "Bringer" strategy: a very fast and highly efficient method for construction of mutant libraries by error-prone polymerase chain reaction of ring-closed plasmids.
Applied biochemistry and biotechnology 2004;117(2):115-22.
2004: Matthias Bureik; Katja Hübel; Calin-Aurel Dragan; Jochen Scher; Hans Becker; Natalie Lenz; Rita Bernhardt
Development of test systems for the discovery of selective human aldosterone synthase (CYP11B2) and 11beta-hydroxylase (CYP11B1) inhibitors. Discovery of a new lead compound for the therapy of congestive heart failure, myocardial fibrosis and hypertension.
Molecular and cellular endocrinology 2004;217(1-2):249-54.
2004: Rita Bernhardt
Optimized chimeragenesis; creating diverse p450 functions.
Chemistry & biology 2004;11(3):287-8.
2004: Michael Lisurek; Rita Bernhardt
Modulation of aldosterone and cortisol synthesis on the molecular level.
Molecular and cellular endocrinology 2004;215(1-2):149-59.
2004: Min-Jung Kang; Michael Lisurek; Rita Bernhardt; Rolf W Hartmann
Use of high-performance liquid chromatography/electrospray ionization collision-induced dissociation mass spectrometry for structural identification of monohydroxylated progesterones.
Rapid communications in mass spectrometry : RCM 2004;18(23):2795-800.
2003: Burkhard Schiffler; Rita Bernhardt
Bacterial (CYP101) and mitochondrial P450 systems-how comparable are they?
Biochemical and biophysical research communications 2003;312(1):223-8.
2003: Angelo Cereda; Fabio Forlani; Stefania Iametti; Rita Bernhardt; Pasquale Ferranti; Gianluca Picariello; Silvia Pagani; Francesco Bonomi
Molecular recognition between Azotobacter vinelandii rhodanese and a sulfur acceptor protein.
Biological chemistry 2003;384(10-11):1473-81.
2003: Branislav Strauch; Tomás Zelinka; Mathias Hampf; Rita Bernhardt; J Widimsky
Prevalence of primary hyperaldosteronism in moderate to severe hypertension in the Central Europe region.
Journal of human hypertension 2003;17(5):349-52.
2003: Merche Faro; Burkhard Schiffler; Achim Heinz; Isabel Nogués; Milagros Medina; Rita Bernhardt; Carlos Gómez-Moreno
Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin.
European journal of biochemistry / FEBS 2003;270(4):726-35.
2002: Matthias Bureik; Annette Zeeh; Rita Bernhardt
Modulation of steroid hydroxylase activity in stably transfected V79MZh11B1 and V79MZh11B2 cells by PKC and PKD inhibitors.
Endocrine research 2002;28(4):351-5.
2002: Matthias Bureik; Michael Lisurek; Rita Bernhardt
The human steroid hydroxylases CYP1B1 and CYP11B2.
Biological chemistry 2002;383(10):1537-51.
2002: Andy Zöllner; Frank Hannemann; Michael Lisurek; Rita Bernhardt
Deletions in the loop surrounding the iron-sulfur cluster of adrenodoxin severely affect the interactions with its native redox partners adrenodoxin reductase and cytochrome P450(scc) (CYP11A1).
Journal of inorganic biochemistry 2002;91(4):644-54.
2002: Frank Hannemann; Aloke Bera; Birgitta Fischer; Michael Lisurek; Klaus Teuchner; Rita Bernhardt
Unfolding and conformational studies on bovine adrenodoxin probed by engineered intrinsic tryptophan fluorescence.
Biochemistry 2002;41(36):11008-16.
2002: Hannes Buelow; Rita Bernhardt
Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity.
European journal of biochemistry / FEBS 2002;269(15):3838-46.
2002: Dirk Beilke; Roland Weiss; Frank Löhr; Primoz Pristovsek; Frank Hannemann; Rita Bernhardt; Heinz Rüterjans
A new electron transport mechanism in mitochondrial steroid hydroxylase systems based on structural changes upon the reduction of adrenodoxin.
Biochemistry 2002;41(25):7969-78.
2002: Peter B Ehmer; Matthias Bureik; Rita Bernhardt; Ursula Müller; Rolf W Hartmann
Development of a test system for inhibitors of human aldosterone synthase (CYP11B2): screening in fission yeast and evaluation of selectivity in V79 cells.
The Journal of steroid biochemistry and molecular biology 2002;81(2):173-9.
2002: Joachim Jose; Rita Bernhardt; Frank Hannemann
Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli.
Journal of biotechnology 2002;95(3):257-68.
2002: Matthias Bureik; Burkhard Schiffler; Yasushi Hiraoka; Frank Vogel; Rita Bernhardt
Functional expression of human mitochondrial CYP11B2 in fission yeast and identification of a new internal electron transfer protein, etp1.
Biochemistry 2002;41(7):2311-21.
2002: Stephanie Bechtel; Natalya Belkina; Rita Bernhardt
The effect of amino-acid substitutions I112P, D147E and K152N in CYP11B2 on the catalytic activities of the enzyme.
European journal of biochemistry / FEBS 2002;269(4):1118-27.
2001: Natalya Belkina; Michael Lisurek; Alexis S Ivanov; Rita Bernhardt
Modelling of three-dimensional structures of cytochromes P450 11B1 and 11B2.
Journal of inorganic biochemistry 2001;87(4):197-207.
2001: AV Grinberg; Rita Bernhardt
Contribution of a salt bridge to the thermostability of adrenodoxin determined by site-directed mutagenesis.
Archives of biochemistry and biophysics 2001;396(1):25-34.
2001: Burkhard Schiffler; Martin Kiefer; A Wilken; Frank Hannemann; Hans-Werner Adolph; Rita Bernhardt
The interaction of bovine adrenodoxin with CYP11A1 (cytochrome P450scc) and CYP11B1 (cytochrome P45011beta ). Acceleration of reduction and substrate conversion by site-directed mutagenesis of adrenodoxin.
The Journal of biological chemistry 2001;276(39):36225-32.
2001: Joachim Jose; Rita Bernhardt; Frank Hannemann
Functional display of active bovine adrenodoxin on the surface of E. coli by chemical incorporation of the [2Fe-2S] cluster.
Chembiochem : a European journal of chemical biology 2001;2(9):695-701.
2001: Mathias Hampf; Nguyen Thi Ngoc Dao; Nguyen Thi Hoan; Rita Bernhardt
Unequal crossing-over between aldosterone synthase and 11beta-hydroxylase genes causes congenital adrenal hyperplasia.
The Journal of clinical endocrinology and metabolism 2001;86(9):4445-52.
2001: Stefania Iametti; Aloke Bera; G Vecchio; A Grinberg; Rita Bernhardt; Francesco Bonomi
GroEL-assisted refolding of adrenodoxin during chemical cluster insertion.
European journal of biochemistry / FEBS 2001;268(8):2421-9.
2001: Frank Hannemann; M Rottmann; Burkhard Schiffler; Josef Zapp; Rita Bernhardt
The loop region covering the iron-sulfur cluster in bovine adrenodoxin comprises a new interaction site for redox partners.
The Journal of biological chemistry 2001;276(2):1369-75.
2000: Mathias Hampf; Nguyen Thi Ngoc Dao; Nguyen Thi Hoan; Rita Bernhardt
New molecular genetic defects causing 11beta-hydroxylase deficiency (CAH).
Endocrine research 2000;26(4):781-2.
2000: AV Grinberg; Frank Hannemann; Burkhard Schiffler; J Müller; Udo Heinemann; Rita Bernhardt
Adrenodoxin: structure, stability, and electron transfer properties.
Proteins 2000;40(4):590-612.
2000: B Simgen; Jörg Contzen; R Schwarzer; Rita Bernhardt; Christiane Jung
Substrate binding to 15beta-hydroxylase (CYP106A2) probed by FT infrared spectroscopic studies of the iron ligand CO stretch vibration.
Biochemical and biophysical research communications 2000;269(3):737-42.
2000: PR Cao; Hannes Buelow; B Dumas; Rita Bernhardt
Construction and characterization of a catalytic fusion protein system: P-450(11beta)-adrenodoxin reductase-adrenodoxin.
Biochimica et biophysica acta 2000;1476(2):253-64.
1999: T Seeman; J Widimský; Mathias Hampf; Rita Bernhardt
Abolished nocturnal blood pressure fall in a boy with glucocorticoid-remediable aldosteronism.
Journal of human hypertension 1999;13(12):823-8.
1999: Jürgen J Müller; A Müller; M Rottmann; Rita Bernhardt; Udo Heinemann
Vertebrate-type and plant-type ferredoxins: crystal structure comparison and electron transfer pathway modelling.
Journal of molecular biology 1999;294(2):501-13.
1999: PR Cao; Rita Bernhardt
Modulation of aldosterone biosynthesis by adrenodoxin mutants with different electron transport efficiencies.
European journal of biochemistry / FEBS 1999;265(1):152-9.
1999: Aloke Bera; Rita Bernhardt
GroEL-assisted and -unassisted refolding of mature and precursor adrenodoxin: the role of the precursor sequence.
Archives of biochemistry and biophysics 1999;367(1):89-94.
1999: PR Cao; Rita Bernhardt
Interaction of CYP11B1 (cytochrome P-45011 beta) with CYP11A1 (cytochrome P-450scc) in COS-1 cells.
European journal of biochemistry / FEBS 1999;262(3):720-6.
1999: Aloke Bera; A Grinberg; Rita Bernhardt
A step toward understanding the folding mechanism of bovine adrenodoxin.
Archives of biochemistry and biophysics 1999;361(2):315-22.
1998: AV Grinberg; Rita Bernhardt
Effect of replacing a conserved proline residue on the function and stability of bovine adrenodoxin.
Protein engineering 1998;11(11):1057-64.
1998: V Goder; Vita Beckert; W Pfeil; Rita Bernhardt
Impact of the presequence of a mitochondrium-targeted precursor, preadrenodoxin, on folding, catalytic activity, and stability of the protein in vitro.
Archives of biochemistry and biophysics 1998;359(1):31-41.
1998: A Grinberg; Rita Bernhardt
Structural and functional consequences of substitutions at the Pro108-Arg14 hydrogen bond in bovine adrenodoxin.
Biochemical and biophysical research communications 1998;249(3):933-7.
1998: Mathias Hampf; J Widimský; Rita Bernhardt
Aldosterone synthase gene in patients suffering from hyperaldosteronism.
Endocrine research 1998;24(3-4):877-80.
1998: Rita Bernhardt; A Müller; H Uhlmann; A Grinberg; Jürgen J Müller; Udo Heinemann
Structure of adrenodoxin and function in mitochondrial steroid hydroxylation.
Endocrine research 1998;24(3-4):531-9.
1998: A Müller; Jürgen J Müller; Yves A Muller; H Uhlmann; Rita Bernhardt; Udo Heinemann
New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108).
Structure (London, England : 1993) 1998;6(3):269-80.
1998: B Böttner; K Denner; Rita Bernhardt
Conferring aldosterone synthesis to human CYP11B1 by replacing key amino acid residues with CYP11B2-specific ones.
European journal of biochemistry / FEBS 1998;252(3):458-66.
1998: Stefania Iametti; H Uhlmann; Enzio Ragg; N Sala; A Grinberg; Vita Beckert; Rita Bernhardt; Francesco Bonomi
Cluster-iron substitution is related to structural and functional features of adrenodoxin mutants and to their redox states.
European journal of biochemistry / FEBS 1998;251(3):673-81.
1997: H Uhlmann; Stefania Iametti; G Vecchio; Francesco Bonomi; Rita Bernhardt
Pro108 is important for folding and stabilization of adrenal ferredoxin, but does not influence the functional properties of the protein.
European journal of biochemistry / FEBS 1997;248(3):897-902.
1997: Vita Beckert; Rita Bernhardt
Specific aspects of electron transfer from adrenodoxin to cytochromes p450scc and p45011beta.
The Journal of biological chemistry 1997;272(8):4883-8.
1996: D Lenz; Rita Bernhardt
Activity of 5'-deletion mutants of the human CYP11B1 promoter in H295R cells.
Endocrine research 1996;22(4):501-2.
1996: Hannes Buelow; Klaus Möbius; Volker Bähr; Rita Bernhardt
Functional expression of the guinea pig 11b-hydroxylase in COS-1 cells.
Endocrine research 1996;22(4):479-84.
1996: B Böttner; Rita Bernhardt
Changed ratios of glucocorticoids/mineralocorticoids caused by point mutations in the putative I-helix regions of CYP11B1 and CYP11B2.
Endocrine research 1996;22(4):455-61.
1996: B Erdmann; H Gerst; Andrea Lippoldt; Hannes Buelow; Detlev Ganten; Kjell Fuxe; Rita Bernhardt
Expression of cytochrome P45011B1 mRNA in the brain of normal and hypertensive transgenic rats.
Brain research 1996;733(1):73-82.
1996: Tatiana V Burova; Vita Beckert; H Uhlmann; Otto Ristau; Rita Bernhardt; W Pfeil
Conformational stability of adrenodoxin mutant proteins.
Protein science : a publication of the Protein Society 1996;5(9):1890-7.
1996: Stefania Iametti; H Uhlmann; N Sala; Rita Bernhardt; Enzio Ragg; Francesco Bonomi
Reversible, non-denaturing metal substitution in bovine adrenodoxin and spinach ferredoxin and the different reactivities of [2Fe-2S]-cluster-containing proteins.
European journal of biochemistry / FEBS 1996;239(3):818-26.
1996: K Denner; William E Rainey; Vincenzo Pezzi; I M Bird; Rita Bernhardt; J Michael Mathis
Differential regulation of 11 beta-hydroxylase and aldosterone synthase in human adrenocortical H295R cells.
Molecular and cellular endocrinology 1996;121(1):87-91.
1996: Hannes Buelow; Klaus Möbius; Volker Bähr; Rita Bernhardt
Molecular cloning and functional expression of the cytochrome P450 11B-hydroxylase of the guinea pig.
Biochemical and biophysical research communications 1996;221(2):304-12.
1996: B Böttner; H Schrauber; Rita Bernhardt
Engineering a mineralocorticoid- to a glucocorticoid-synthesizing cytochrome P450.
The Journal of biological chemistry 1996;271(14):8028-33.
1995: H Uhlmann; Rita Bernhardt
The role of threonine 54 in adrenodoxin for the properties of its iron-sulfur cluster and its electron transfer function.
The Journal of biological chemistry 1995;270(50):29959-66.
1995: B Erdmann; H Gerst; Hannes Buelow; D Lenz; Volker Bähr; Rita Bernhardt
Zone-specific localization of cytochrome P45011B1 in human adrenal tissue by PCR-derived riboprobes.
Histochemistry and cell biology 1995;104(4):301-7.
1995: Vita Beckert; H Schrauber; Rita Bernhardt; A A Van Dijk; C Kakoschke; Victor Wray
Mutational effects on the spectroscopic properties and biological activities of oxidized bovine adrenodoxin, and their structural implications.
European journal of biochemistry / FEBS 1995;231(1):226-35.
1995: Christoph Grunau; R Dettmer; Joachim Behlke; Rita Bernhardt
Bovine adrenodoxin--a mitochondrial iron-sulphur protein--binds to chaperonin GroEL.
Biochemical and biophysical research communications 1995;210(3):1001-8.
1995: Tatiana V Burova; Rita Bernhardt; W Pfeil
Conformational stability of bovine holo and apo adrenodoxin--a scanning calorimetric study.
Protein science : a publication of the Protein Society 1995;4(5):909-16.
1995: K Denner; R Vogel; W Schmalix; Johannes Doehmer; Rita Bernhardt
Cloning and stable expression of the human mitochondrial cytochrome P45011B1 cDNA in V79 Chinese hamster cells and their application for testing of potential inhibitors.
Pharmacogenetics 1995;5(2):89-96.
1995: Stephan Geley; Karin Jöhrer; Michael Peter; K Denner; Rita Bernhardt; Wolfgang G Sippell; Reinhard Kofler
Amino acid substitution R384P in aldosterone synthase causes corticosterone methyloxidase type I deficiency.
The Journal of clinical endocrinology and metabolism 1995;80(2):424-9.
1995: K Denner; Johannes Doehmer; Rita Bernhardt
Cloning of CYP11B1 and CYP11B2 from normal human adrenal and their functional expression in COS-7 and V79 Chinese hamster cells.
Endocrine research 1995;21(1-2):443-8.
1995: B Erdmann; K Denner; H Gerst; D Lenz; Rita Bernhardt
Human adrenal CYP11B1: localization by in situ-hybridization and functional expression in cell cultures.
Endocrine research 1995;21(1-2):425-35.
1995: H Uhlmann; Rita Bernhardt
Structure-function studies on mutants of adrenal ferredoxin.
Endocrine research 1995;21(1-2):307-12.
1994: B Erdmann; M Henning; H Uhlmann; Vita Beckert; Christoph Grunau; Rita Bernhardt
Immunocytochemical localization of heterologously expressed adrenodoxin and its electron acceptor cytochrome P45011B1 in Escherichia coli.
European journal of cell biology 1994;65(1):220-8.
1994: H Uhlmann; R Kraft; Rita Bernhardt
C-terminal region of adrenodoxin affects its structural integrity and determines differences in its electron transfer function to cytochrome P-450.
The Journal of biological chemistry 1994;269(36):22557-64.
1994: Vita Beckert; R Dettmer; Rita Bernhardt
Mutations of tyrosine 82 in bovine adrenodoxin that affect binding to cytochromes P45011A1 and P45011B1 but not electron transfer.
The Journal of biological chemistry 1994;269(4):2568-73.
1992: H Uhlmann; Vita Beckert; Dieter Schwarz; Rita Bernhardt
Expression of bovine adrenodoxin in E. coli and site-directed mutagenesis of /2 Fe-2S/ cluster ligands.
Biochemical and biophysical research communications 1992;188(3):1131-8.