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Eric Phizicky
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Network (preview)
17
Grayhack, Elizabeth
9
Jackman, Jane
8
Spinelli, Sherry
6
Alexandrov, Andrei
5
Consaul, Sandra
5
Martzen, Mark
4
Kierzek, Ryszard
4
deTitta, George
4
Quartley, Erin
4
Turner, Douglas
3
Xing, Feng
3
Steiger, Michelle
3
Shull, Neil
3
Fields, Stanley
3
Gu, Weifeng
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Geonetwork of Eric Phizicky (preview)
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All Publications
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2009: Quartley Erin; Alexandrov Andrei; Mikucki Maryann; Buckner Frederick S; Hol Wim G; DeTitta George T; Phizicky Eric M; Grayhack Elizabeth J
Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.
Journal of structural and functional genomics 2009;10(3):233-47.
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2008: Phizicky Eric M
Waste not, want not: a case for tRNA repair.
Nature structural & molecular biology 2008;15(8):783-4.
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2008: Merritt Ethan A; Holmes Margaret; Buckner Frederick S; Van Voorhis Wesley C; Quartly Erin; Phizicky Eric M; Lauricella Angela; Luft Joseph; DeTitta George; Neely Helen; Zucker Frank; Hol Wim G J
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 6):474-8.
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2008: Chernyakov Irina; Whipple Joseph M; Kotelawala Lakmal; Grayhack Elizabeth J; Phizicky Eric M
Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.
Genes & development 2008;22(10):1369-80.
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2008: Jackman Jane E; Phizicky Eric M
Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase.
Biochemistry 2008;47(16):4817-25.
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2008: Kotelawala Lakmal; Grayhack Elizabeth J; Phizicky Eric M
Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.
RNA (New York, N.Y.) 2008;14(1):158-69.
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2008: Chernyakov Irina; Baker Melanie A; Grayhack Elizabeth J; Phizicky Eric M
Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.
Methods in enzymology 2008;449():221-37.
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2008: Jackman Jane E; Grayhack Elizabeth J; Phizicky Eric M
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.
Methods in molecular biology (Clifton, N.J.) 2008;488():383-93.
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2007: Malkowski Michael G; Quartley Erin; Friedman Alan E; Babulski Julie; Kon Yoshiko; Wolfley Jennifer; Said Meriem; Luft Joseph R; Phizicky Eric M; DeTitta George T; Grayhack Elizabeth J
Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(16):6678-83.
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2007: Wilkinson Martha L; Crary Sharon M; Jackman Jane E; Grayhack Elizabeth J; Phizicky Eric M
The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.
RNA (New York, N.Y.) 2007;13(3):404-13.
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2007: Jackman Jane E; Kotelawala Lakmal; Grayhack Elizabeth J; Phizicky Eric M
Identification and characterization of modification enzymes by biochemical analysis of the proteome.
Methods in enzymology 2007;425():139-52.
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2006: Phizicky Eric M; Grayhack Elizabeth J
Proteome-scale analysis of biochemical activity.
Critical reviews in biochemistry and molecular biology 2006;41(5):315-27.
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2006: Jackman Jane E; Phizicky Eric M
tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(23):8640-5.
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2006: Jackman Jane E; Phizicky Eric M
tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases.
RNA (New York, N.Y.) 2006;12(6):1007-14.
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2006: Arakaki Tracy; Le Trong Isolde; Phizicky Eric; Quartley Erin; DeTitta George; Luft Joseph; Lauricella Angela; Anderson Lori; Kalyuzhniy Oleksandr; Worthey Elizabeth; Myler Peter J; Kim David; Baker David; Hol Wim G J; Merritt Ethan A
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 3):175-9.
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2006: Alexandrov Andrei; Chernyakov Irina; Gu Weifeng; Hiley Shawna L; Hughes Timothy R; Grayhack Elizabeth J; Phizicky Eric M
Rapid tRNA decay can result from lack of nonessential modifications.
Molecular cell 2006;21(1):87-96.
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2005: Shull Neil P; Spinelli Sherry L; Phizicky Eric M
We are writing to express our concerns regarding the recently published article by Kumaran et al.
Protein science : a publication of the Protein Society 2005;14(12):3145; author reply 3145.
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2005: Gelperin Daniel M; White Michael A; Wilkinson Martha L; Kon Yoshiko; Kung Li A; Wise Kevin J; Lopez-Hoyo Nelson; Jiang Lixia; Piccirillo Stacy; Yu Haiyuan; Gerstein Mark; Dumont Mark E; Phizicky Eric M; Snyder Michael; Grayhack Elizabeth J
Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.
Genes & development 2005;19(23):2816-26.
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2005: Gu Weifeng; Hurto Rebecca L; Hopper Anita K; Grayhack Elizabeth J; Phizicky Eric M
Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.
Molecular and cellular biology 2005;25(18):8191-201.
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2005: Phizicky Eric M
Have tRNA, will travel.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(32):11127-8.
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2005: Cartlidge Robert A; Knebel Axel; Peggie Mark; Alexandrov Andrei; Phizicky Eric M; Cohen Philip
The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells.
The EMBO journal 2005;24(9):1696-705.
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2005: Alexandrov Andrei; Grayhack Elizabeth J; Phizicky Eric M
tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.
RNA (New York, N.Y.) 2005;11(5):821-30.
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2005: Steiger Michelle A; Jackman Jane E; Phizicky Eric M
Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
RNA (New York, N.Y.) 2005;11(1):99-106.
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2005: Shull Neil P; Spinelli Sherry L; Phizicky Eric M
A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae.
Nucleic acids research 2005;33(2):650-60.
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2005: Hiley Shawna L; Jackman Jane; Babak Tomas; Trochesset Miles; Morris Quaid D; Phizicky Eric; Hughes Timothy R
Detection and discovery of RNA modifications using microarrays.
Nucleic acids research 2005;33(1):e2.
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2004: Alexandrov Andrei; Vignali Marissa; LaCount Douglas J; Quartley Erin; de Vries Christina; De Rosa Daniela; Babulski Julie; Mitchell Sarah F; Schoenfeld Lori W; Fields Stanley; Hol Wim G; Dumont Mark E; Phizicky Eric M; Grayhack Elizabeth J
A facile method for high-throughput co-expression of protein pairs.
Molecular & cellular proteomics : MCP 2004;3(9):934-8.
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2004: Xing Feng; Hiley Shawna L; Hughes Timothy R; Phizicky Eric M
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs.
The Journal of biological chemistry 2004;279(17):17850-60.
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2003: Gu Weifeng; Jackman Jane E; Lohan Amanda J; Gray Michael W; Phizicky Eric M
tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis.
Genes & development 2003;17(23):2889-901.
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2003: Jackman Jane E; Montange Rebecca K; Malik Harmit S; Phizicky Eric M
Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9.
RNA (New York, N.Y.) 2003;9(5):574-85.
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2003: Phizicky Eric; Bastiaens Philippe I H; Zhu Heng; Snyder Michael; Fields Stanley
Protein analysis on a proteomic scale.
Nature 2003;422(6928):208-15.
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2003: Hopper Anita K; Phizicky Eric M
tRNA transfers to the limelight.
Genes & development 2003;17(2):162-80.
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2002: Alexandrov Andrei; Martzen Mark R; Phizicky Eric M
Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA.
RNA (New York, N.Y.) 2002;8(10):1253-66.
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2002: Xing Feng; Martzen Mark R; Phizicky Eric M
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA.
RNA (New York, N.Y.) 2002;8(3):370-81.
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2002: Phizicky Eric M; Martzen Mark R; McCraith Stephen M; Spinelli Sherry L; Xing Feng; Shull Neil P; Van Slyke Ceri; Montagne Rebecca K; Torres Francy M; Fields Stanley; Grayhack Elizabeth J
Biochemical genomics approach to map activities to genes.
Methods in enzymology 2002;350():546-59.
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2001: Steiger M A; Kierzek R; Turner D H; Phizicky E M
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog.
Biochemistry 2001;40(46):14098-105.
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2001: Grayhack E J; Phizicky E M
Genomic analysis of biochemical function.
Current opinion in chemical biology 2001;5(1):34-9.
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2000: Polevoda B; Martzen M R; Das B; Phizicky E M; Sherman F
Cytochrome c methyltransferase, Ctm1p, of yeast.
The Journal of biological chemistry 2000;275(27):20508-13.
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2000: Kierzek R; Steiger M A; Spinelli S L; Turner D H; Phizicky E M
The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates.
Nucleosides, nucleotides & nucleic acids 2000;19(5-6):917-33.
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1999: Martzen M R; McCraith S M; Spinelli S L; Torres F M; Fields S; Grayhack E J; Phizicky E M
A biochemical genomics approach for identifying genes by the activity of their products.
Science (New York, N.Y.) 1999;286(5442):1153-5.
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1999: Spinelli S L; Kierzek R; Turner D H; Phizicky E M
Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast.
The Journal of biological chemistry 1999;274(5):2637-44.
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1998: Spinelli S L; Malik H S; Consaul S A; Phizicky E M
A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(24):14136-41.
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1998: Robinson M K; Phizicky E M
Purification and assay of the Ptc1/Tpd1 protein phosphatase 2C from the yeast Saccharomyces cerevisiae.
Methods in molecular biology (Clifton, N.J.) 1998;93():235-42.
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1997: Spinelli S L; Consaul S A; Phizicky E M
A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction.
RNA (New York, N.Y.) 1997;3(12):1388-400.
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1997: Culver G M; McCraith S M; Consaul S A; Stanford D R; Phizicky E M
A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae.
The Journal of biological chemistry 1997;272(20):13203-10.
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1995: Phizicky E M; Fields S
Protein-protein interactions: methods for detection and analysis.
Microbiological reviews 1995;59(1):94-123.
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1994: Culver G M; Consaul S A; Tycowski K T; Filipowicz W; Phizicky E M
tRNA splicing in yeast and wheat germ. A cyclic phosphodiesterase implicated in the metabolism of ADP-ribose 1",2"-cyclic phosphate.
The Journal of biological chemistry 1994;269(40):24928-34.
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1994: Robinson M K; van Zyl W H; Phizicky E M; Broach J R
TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation.
Molecular and cellular biology 1994;14(6):3634-45.
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1993: Culver G M; McCraith S M; Zillmann M; Kierzek R; Michaud N; LaReau R D; Turner D H; Phizicky E M
An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate.
Science (New York, N.Y.) 1993;261(5118):206-8.
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1993: Phizicky E M; Greer C L
Pre-tRNA splicing: variation on a theme or exception to the rule?
Trends in biochemical sciences 1993;18(1):31-4.
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1992: Zillmann M; Gorovsky M A; Phizicky E M
HeLa cells contain a 2'-phosphate-specific phosphotransferase similar to a yeast enzyme implicated in tRNA splicing.
The Journal of biological chemistry 1992;267(15):10289-94.
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1992: Phizicky E M; Consaul S A; Nehrke K W; Abelson J
Yeast tRNA ligase mutants are nonviable and accumulate tRNA splicing intermediates.
The Journal of biological chemistry 1992;267(7):4577-82.
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1991: Zillmann M; Gorovsky M A; Phizicky E M
Conserved mechanism of tRNA splicing in eukaryotes.
Molecular and cellular biology 1991;11(11):5410-6.
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1991: McCraith S M; Phizicky E M
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
The Journal of biological chemistry 1991;266(18):11986-92.
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1990: McCraith S M; Phizicky E M
A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.
Molecular and cellular biology 1990;10(3):1049-55.
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1990: Xu Q; Phizicky E M; Greer C L; Abelson J N
Purification of yeast transfer RNA ligase.
Methods in enzymology 1990;181():463-71.
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