Username

Password

Forgot Password?

Preview

Sign-in free and Explore the Exciting World of BiomedExperts:

Over 1.500.000 Profiles
More than 1.800 Organizations worldwide
State of the Art Network Visualizations

Manage your own Profile
Locate Experts in your Country/Region
Locate Experts in your 1. and 2. Level Network
Connect to Experts Worldwide

NetworkView

Alfred Pingoud

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

Research Profile (preview)

Chemicals & Drugs

Concepts & Ideas

Living Beings

Escherichia coli

Genes & Molecular Sequences

Amino Acid Sequence

Physiology

Site-Directed Mutagenesis

Phenomena

Procedures

Polymerase Chain Reaction

Sign-in to see full Profile

Network (preview)

Co-Publications
Name
34
Jeltsch, Albert
27
Wende, Wolfgang
22
Meiss, Gregor
21
Friedhoff, Peter
18
Pingoud, Vera
17
Wolfes, Heiner
17
Gimadutdinow, Oleg
16
Alves, Jürgen
14
Urbanke, Claus
11
Fliess, Anja
11
Gast, Frank-Ulrich
11
Pieper, Uwe
9
Lanio, Thomas
9
Franke, I
9
Wilhelm, Jochen

Sign-in to see all Coauthors

Publications

TOP 3
Pingoud Vera; Wende Wolfgang; Friedhoff Peter; Reuter Monika; Alves Jürgen; Jeltsch Albert; Mones Letif; Fuxreiter Monika; Pingoud Alfred
On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Khien Le Tkhi; Shirling B; Ryazanova A Yu; Zatsepin T S; Volkov E M; Kubareva E A; Velichko T I; Pingoud A; Oretskaya T S
[New derivatives of azobenzene for the directed modification of proteins.]
Biebricher Andreas; Wende Wolfgang; Escudé Christophe; Pingoud Alfred; Desbiolles Pierre
Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.

Sign-in to see all Publications

Sign in free and see...

Visualized networks:

See your personal network in
sophisticated graphical views

GeoTargeted Searches:

Locate experts around the world
and connect with global collaborators

Research Profiles:

See the visualized research activity
of experts around the globe

Sign-in to see more

Geonetwork of Alfred Pingoud (preview)

Cities where this author has publications
Cities where co-authors of this author have publications

Flash Player Required

Sign-in to see more

All Publications

2009: Pingoud Vera; Wende Wolfgang; Friedhoff Peter; Reuter Monika; Alves Jürgen; Jeltsch Albert; Mones Letif; Fuxreiter Monika; Pingoud Alfred
On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Journal of molecular biology 2009;393(1):140-60.
2009: Khien Le Tkhi; Shirling B; Ryazanova A Yu; Zatsepin T S; Volkov E M; Kubareva E A; Velichko T I; Pingoud A; Oretskaya T S
[New derivatives of azobenzene for the directed modification of proteins.]
Bioorganicheskaia khimiia 2009;35(5):610-7.
2009: Biebricher Andreas; Wende Wolfgang; Escudé Christophe; Pingoud Alfred; Desbiolles Pierre
Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.
Biophysical journal 2009;96(8):L50-2.
2008: Bonnet Isabelle; Biebricher Andreas; Porté Pierre-Louis; Loverdo Claude; Bénichou Olivier; Voituriez Raphaël; Escudé Christophe; Wende Wolfgang; Pingoud Alfred; Desbiolles Pierre
Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA.
Nucleic acids research 2008;36(12):4118-27.
2007: Pingoud Alfred; Silva George H
Precision genome surgery.
Nature biotechnology 2007;25(7):743-4.
2007: Pingoud Alfred; Wende Wolfgang
A sliding restriction enzyme pauses.
Structure (London, England : 1993) 2007;15(4):391-3.
2007: Ghosh Mahua; Meiss Gregor; Pingoud Alfred M; London Robert E; Pedersen Lars C
The nuclease a-inhibitor complex is characterized by a novel metal ion bridge.
The Journal of biological chemistry 2007;282(8):5682-90.
2007: Rathert Philipp; Raskó Tamás; Roth Markus; Slaska-Kiss Krystyna; Pingoud Alfred; Kiss Antal; Jeltsch Albert
Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group.
Chembiochem : a European journal of chemical biology 2007;8(2):202-7.
2007: Friedhoff Peter; Pingoud Alfred
Engineering site-specific endonucleases.
Methods in molecular biology (Clifton, N.J.) 2007;352():111-23.
2006: Silva George H; Belfort Marlene; Wende Wolfgang; Pingoud Alfred
From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes.
Journal of molecular biology 2006;361(4):744-54.
2005: Sud'ina A E; Zatsepin T S; Pingoud V; Pingoud A; Oretskaya T S; Kubareva E A
Affinity modification of the restriction endonuclease SsoII by 2'-aldehyde-containing double stranded DNAs.
Biochemistry. Biokhimii?a 2005;70(8):941-7.
2005: Pingoud Vera; Geyer Hildegard; Geyer Rudolf; Kubareva Elena; Bujnicki Janusz M; Pingoud Alfred
Identification of base-specific contacts in protein-DNA complexes by photocrosslinking and mass spectrometry: a case study using the restriction endonuclease SsoII.
Molecular bioSystems 2005;1(2):135-41.
2005: Ghosh Mahua; Meiss Gregor; Pingoud Alfred; London Robert E; Pedersen Lars C
Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena.
The Journal of biological chemistry 2005;280(30):27990-7.
2005: Pingoud A; Fuxreiter M; Pingoud V; Wende W
Type II restriction endonucleases: structure and mechanism.
Cellular and molecular life sciences : CMLS 2005;62(6):685-707.
2005: Korn Christian; Scholz Sebastian R; Gimadutdinow Oleg; Lurz Rudi; Pingoud Alfred; Meiss Gregor
Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
The Journal of biological chemistry 2005;280(7):6005-15.
2005: Pingoud Vera; Sudina Anna; Geyer Hildegard; Bujnicki Janusz M; Lurz Rudi; Lüder Gerhild; Morgan Richard; Kubareva Elena; Pingoud Alfred
Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences.
The Journal of biological chemistry 2005;280(6):4289-98.
2005: Eisenschmidt Kristin; Lanio Thomas; Simoncsits András; Jeltsch Albert; Pingoud Vera; Wende Wolfgang; Pingoud Alfred
Developing a programmed restriction endonuclease for highly specific DNA cleavage.
Nucleic acids research 2005;33(22):7039-47.
2004: Schäfer Patrick; Scholz Sebastian R; Gimadutdinow Oleg; Cymerman Iwona A; Bujnicki Janusz M; Ruiz-Carrillo Adolf; Pingoud Alfred; Meiss Gregor
Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis.
Journal of molecular biology 2004;338(2):217-28.
2004: Steuer Shawn; Pingoud Vera; Pingoud Alfred; Wende Wolfgang
Chimeras of the homing endonuclease PI-SceI and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity.
Chembiochem : a European journal of chemical biology 2004;5(2):206-13.
2004: Noël Ann-Josée; Wende Wolfgang; Pingoud Alfred
DNA recognition by the homing endonuclease PI-SceI involves a divalent metal ion cofactor-induced conformational change.
The Journal of biological chemistry 2004;279(8):6794-804.
2003: Wilhelm Jochen; Pingoud Alfred
Real-time polymerase chain reaction.
Chembiochem : a European journal of chemical biology 2003;4(11):1120-8.
2003: Tews Björn; Wilhelm Jochen; Summerer Daniel; Strerath Michael; Marx Andreas; Friedhoff Peter; Pingoud Alfred; Hahn Meinhard
Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants.
Biological chemistry 2003;384(10-11):1533-41.
2003: Scholz Sebastian R; Korn Christian; Bujnicki Janusz M; Gimadutdinow Oleg; Pingoud Alfred; Meiss Gregor
Experimental evidence for a beta beta alpha-Me-finger nuclease motif to represent the active site of the caspase-activated DNase.
Biochemistry 2003;42(31):9288-94.
2003: Spyridaki Aspasia; Matzen Claudia; Lanio Thomas; Jeltsch Albert; Simoncsits Andras; Athanasiadis Alekos; Scheuring-Vanamee Eva; Kokkinidis Michael; Pingoud Alfred
Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII.
Journal of molecular biology 2003;331(2):395-406.
2003: Pingoud Vera; Conzelmann Charlotte; Kinzebach Steffen; Sudina Anna; Metelev Valeri; Kubareva Elena; Bujnicki Janusz M; Lurz Rudi; Lüder Gerhild; Xu Shuang-Yong; Pingoud Alfred
PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes.
Journal of molecular biology 2003;329(5):913-29.
2003: Wilhelm Jochen; Pingoud Alfred; Hahn Meinhard
Validation of an algorithm for automatic quantification of nucleic acid copy numbers by real-time polymerase chain reaction.
Analytical biochemistry 2003;317(2):218-25.
2003: Wilhelm Jochen; Pingoud Alfred; Hahn Meinhard
Real-time PCR-based method for the estimation of genome sizes.
Nucleic acids research 2003;31(10):e56.
2003: Wilhelm Jochen; Pingoud Alfred; Hahn Meinhard
SoFAR: software for fully automatic evaluation of real-time PCR data.
BioTechniques 2003;34(2):324-32.
2003: Königshoff Melanie; Wilhelm Jochen; Bohle Rainer M; Pingoud Alfred; Hahn Meinhard
HER-2/neu gene copy number quantified by real-time PCR: comparison of gene amplification, heterozygosity, and immunohistochemical status in breast cancer tissue.
Clinical chemistry 2003;49(2):219-29.
2003: Friedhoff Peter; Thomas Evangelos; Pingoud Alfred
Tyr212: a key residue involved in strand discrimination by the DNA mismatch repair endonuclease MutH.
Journal of molecular biology 2003;325(2):285-97.
2003: Lanio Thomas; Jeltsch Albert; Pingoud Alfred
High-throughput purification of polyHis-tagged recombinant fusion proteins.
Methods in molecular biology (Clifton, N.J.) 2003;205():199-203.
2002: Thomas Evangelos; Pingoud Alfred; Friedhoff Peter
An efficient method for the preparation of long heteroduplex DNA as substrate for mismatch repair by the Escherichia coli MutHLS system.
Biological chemistry 2002;383(9):1459-62.
2002: Wilhelm Jochen; Reuter Hendrik; Tews Björn; Pingoud Alfred; Hahn Meinhard
Detection and quantification of insertion/deletion variations by allele-specific real-time PCR: application for genotyping and chimerism analysis.
Biological chemistry 2002;383(9):1423-33.
2002: Werner Erik; Wende Wolfgang; Pingoud Alfred; Heinemann Udo
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.
Nucleic acids research 2002;30(18):3962-71.
2002: Scholz Sebastian Richard; Korn Christian; Gimadutdinow Oleg; Knoblauch Michael; Pingoud Alfred; Meiss Gregor
The effect of ICAD-S on the formation and intracellular distribution of a nucleolytically active caspase-activated DNase.
Nucleic acids research 2002;30(14):3045-51.
2002: Kirby Thomas W; Mueller Geoffrey A; DeRose Eugene F; Lebetkin Mark S; Meiss Gregor; Pingoud Alfred; London Robert E
The nuclease A inhibitor represents a new variation of the rare PR-1 fold.
Journal of molecular biology 2002;320(4):771-82.
2002: Eisenschmidt Kristin; Lanio Thomas; Jeltsch Albert; Pingoud Alfred
A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleases.
Journal of biotechnology 2002;96(2):185-91.
2002: Pieper Uwe; Groll Detlef H; Wünsch Sandra; Gast Frank-Ulrich; Speck Christian; Mücke Norbert; Pingoud Alfred
The GTP-dependent restriction enzyme McrBC from Escherichia coli forms high-molecular mass complexes with DNA and produces a cleavage pattern with a characteristic 10-base pair repeat.
Biochemistry 2002;41(16):5245-54.
2002: Pieper Uwe; Pingoud Alfred
A mutational analysis of the PD...D/EXK motif suggests that McrC harbors the catalytic center for DNA cleavage by the GTP-dependent restriction enzyme McrBC from Escherichia coli.
Biochemistry 2002;41(16):5236-44.
2002: Pingoud Vera; Kubareva Elena; Stengel Gudrun; Friedhoff Peter; Bujnicki Janusz M; Urbanke Claus; Sudina Anna; Pingoud Alfred
Evolutionary relationship between different subgroups of restriction endonucleases.
The Journal of biological chemistry 2002;277(16):14306-14.
2002: Korn Christian; Scholz Sebastian Richard; Gimadutdinow Oleg; Pingoud Alfred; Meiss Gregor
Involvement of conserved histidine, lysine and tyrosine residues in the mechanism of DNA cleavage by the caspase-3 activated DNase CAD.
Nucleic acids research 2002;30(6):1325-32.
2002: Friedhoff Peter; Sheybani Babak; Thomas Evangelos; Merz Christian; Pingoud Alfred
Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli.
FEMS microbiology letters 2002;208(1):123-8.
2001: Meiss G; Scholz S R; Korn C; Gimadutdinow O; Pingoud A
Identification of functionally relevant histidine residues in the apoptotic nuclease CAD.
Nucleic acids research 2001;29(19):3901-9.
2001: Pingoud A; Jeltsch A
Structure and function of type II restriction endonucleases.
Nucleic acids research 2001;29(18):3705-27.
2001: Gast F U; Pingoud A
Nucleic acid quantification by delta pH measurement.
Journal of biotechnology 2001;88(2):107-17.
2001: Friedhoff P; Lurz R; Lüder G; Pingoud A
Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops.
The Journal of biological chemistry 2001;276(26):23581-8.
2001: Wilhelm J; Pingoud A; Hahn M
Comparison between Taq DNA polymerase and its Stoffel fragment for quantitative real-time PCR with hybridization probes.
BioTechniques 2001;30(5):1052-6, 1058, 1060 passim.
2001: Gast F U; Franke I; Meiss G; Pingoud A
Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction.
Journal of biotechnology 2001;87(2):131-41.
2001: Meiss G; Gimadutdinow O; Friedhoff P; Pingoud A M
Microtiter-plate assay and related assays for nonspecific endonucleases.
Methods in molecular biology (Clifton, N.J.) 2001;160():37-48.
2001: Jeltsch A; Pingoud A M
Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases.
Methods in molecular biology (Clifton, N.J.) 2001;160():287-308.
2000: Schöttler S; Wende W; Pingoud V; Pingoud A
Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI.
Biochemistry 2000;39(51):15895-900.
2000: Wende W; Schöttler S; Grindl W; Christ F; Steuer S; Nöel A J; Pingoud V; Pingoud A
[Analysis of binding and cleavage of DNA by the gene conversion PI-SCEI endonuclease using site-directed mutagenesis]
Molekuliarnaia biologiia 2000;34(6):1054-64.
2000: Wilhelm J; Hahn M; Pingoud A
Influence of DNA target melting behavior on real-time PCR quantification.
Clinical chemistry 2000;46(11):1738-43.
2000: Meiss G; Böttcher U; Korn C; Gimadutdinow O; Pingoud A
Vectors for dual expression of target genes in bacterial and mammalian cells.
BioTechniques 2000;29(3):476-8, 480.
2000: Lanio T; Jeltsch A; Pingoud A
Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis.
BioTechniques 2000;29(2):338-42.
2000: Korn C; Meiss G; Gast F; Gimadutdinow O; Urbanke C; Pingoud A
Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.
Gene 2000;253(2):221-9.
2000: Christ F; Steuer S; Thole H; Wende W; Pingoud A; Pingoud V
A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links.
Journal of molecular biology 2000;300(4):867-75.
2000: Lanio T; Jeltsch A; Pingoud A
On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target.
Protein engineering 2000;13(4):275-81.
2000: Meiss G; Gimadutdinow O; Haberland B; Pingoud A
Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA.
Journal of molecular biology 2000;297(2):521-34.
2000: Kubareva E A; Thole H; Karyagina A S; Oretskaya T S; Pingoud A; Pingoud V
Identification of a base-specific contact between the restriction endonuclease SsoII and its recognition sequence by photocross-linking.
Nucleic acids research 2000;28(5):1085-91.
1999: Christ F; Schoettler S; Wende W; Steuer S; Pingoud A; Pingoud V
The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate.
The EMBO journal 1999;18(24):6908-16.
1999: Pieper U; Schweitzer T; Groll D H; Pingoud A
Defining the location and function of domains of McrB by deletion mutagenesis.
Biological chemistry 1999;380(10):1225-30.
1999: Srivastava T K; Friedhoff P; Pingoud A; Katti S B
Application of oligonucleoside methylphosphonates in the studies on phosphodiester hydrolysis by Serratia endonuclease.
Nucleosides & nucleotides 1999;18(9):1945-60.
1999: Pieper U; Schweitzer T; Groll D H; Gast F U; Pingoud A
The GTP-binding domain of McrB: more than just a variation on a common theme?
Journal of molecular biology 1999;292(3):547-56.
1999: Meiss G; Gast F U; Pingoud A M
The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates.
Journal of molecular biology 1999;288(3):377-90.
1999: Pingoud V; Thole H; Christ F; Grindl W; Wende W; Pingoud A
Photocross-linking of the homing endonuclease PI-SceI to its recognition sequence.
The Journal of biological chemistry 1999;274(15):10235-43.
1999: Friedhoff P; Franke I; Meiss G; Wende W; Krause K L; Pingoud A
A similar active site for non-specific and specific endonucleases.
Nature structural biology 1999;6(2):112-3.
1999: Franke I; Pingoud A
Synthesis and biochemical characterization of obligatory dimers of the sugar non-specific nuclease from Serratia marcescens using specifically designed bismaleimidoalkanes as SH-specific crosslinking reagents.
Journal of protein chemistry 1999;18(1):137-46.
1999: Friedhoff P; Franke I; Krause K L; Pingoud A
Cleavage experiments with deoxythymidine 3',5'-bis-(p-nitrophenyl phosphate) suggest that the homing endonuclease I-PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non-specific Serratia nuclease.
FEBS letters 1999;443(2):209-14.
1999: Franke I; Meiss G; Pingoud A
On the advantage of being a dimer, a case study using the dimeric Serratia nuclease and the monomeric nuclease from Anabaena sp. strain PCC 7120.
The Journal of biological chemistry 1999;274(2):825-32.
1998: Schöttler S; Wenz C; Lanio T; Jeltsch A; Pingoud A
Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site.
European journal of biochemistry / FEBS 1998;258(1):184-91.
1998: Schulze C; Jeltsch A; Franke I; Urbanke C; Pingoud A
Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover.
The EMBO journal 1998;17(22):6757-66.
1998: Pingoud V; Grindl W; Wende W; Thole H; Pingoud A
Structural and functional analysis of the homing endonuclease PI-sceI by limited proteolytic cleavage and molecular cloning of partial digestion products.
Biochemistry 1998;37(22):8233-43.
1998: Stahl F; Wende W; Jeltsch A; Pingoud A
The mechanism of DNA cleavage by the type II restriction enzyme EcoRV: Asp36 is not directly involved in DNA cleavage but serves to couple indirect readout to catalysis.
Biological chemistry 1998;379(4-5):467-73.
1998: Franke I; Meiss G; Blecher D; Gimadutdinow O; Urbanke C; Pingoud A
Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease.
FEBS letters 1998;425(3):517-22.
1998: Stahl F; Wende W; Wenz C; Jeltsch A; Pingoud A
Intra- vs intersubunit communication in the homodimeric restriction enzyme EcoRV: Thr 37 and Lys 38 involved in indirect readout are only important for the catalytic activity of their own subunit.
Biochemistry 1998;37(16):5682-8.
1998: Grindl W; Wende W; Pingoud V; Pingoud A
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.
Nucleic acids research 1998;26(8):1857-62.
1998: Meiss G; Franke I; Gimadutdinow O; Urbanke C; Pingoud A
Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA.
European journal of biochemistry / FEBS 1998;251(3):924-34.
1998: Wenz C; Hahn M; Pingoud A
Engineering of variants of the restriction endonuclease EcoRV that depend in their cleavage activity on the flexibility of sequences flanking the recognition site.
Biochemistry 1998;37(8):2234-42.
1998: Jeltsch A; Pingoud A
Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA.
Biochemistry 1998;37(8):2160-9.
1998: Lanio T; Jeltsch A; Pingoud A
Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude.
Journal of molecular biology 1998;283(1):59-69.
1997: Gast F U; Brinkmann T; Pieper U; Krüger T; Noyer-Weidner M; Pingoud A
The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB.
Biological chemistry 1997;378(9):975-82.
1997: Pieper U; Brinkmann T; Krüger T; Noyer-Weidner M; Pingoud A
Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP.
Journal of molecular biology 1997;272(2):190-9.
1997: Groll D H; Jeltsch A; Selent U; Pingoud A
Does the restriction endonuclease EcoRV employ a two-metal-Ion mechanism for DNA cleavage?
Biochemistry 1997;36(38):11389-401.
1997: Pingoud A; Jeltsch A
Recognition and cleavage of DNA by type-II restriction endonucleases.
European journal of biochemistry / FEBS 1997;246(1):1-22.
1996: Lanio T; Selent U; Wenz C; Wende W; Schulz A; Adiraj M; Katti S B; Pingoud A
EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodeoxynucleotides.
Protein engineering 1996;9(11):1005-10.
1996: Kolmes B; Franke I; Friedhoff P; Pingoud A
Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial minimal substrate.
FEBS letters 1996;397(2-3):343-6.
1996: Wende W; Grindl W; Christ F; Pingoud A; Pingoud V
Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI.
Nucleic acids research 1996;24(21):4123-32.
1996: Wende W; Stahl F; Pingoud A
The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV.
Biological chemistry 1996;377(10):625-32.
1996: Friedhoff P; Meiss G; Kolmes B; Pieper U; Gimadutdinow O; Urbanke C; Pingoud A
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease.
European journal of biochemistry / FEBS 1996;241(2):572-80.
1996: Jeltsch A; Wenz C; Stahl F; Pingoud A
Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme.
The EMBO journal 1996;15(18):5104-11.
1996: Friedhoff P; Matzen S E; Meiss G; Pingoud A
A quantitative microtiter plate nuclease assay based on ethidium/DNA fluorescence.
Analytical biochemistry 1996;240(2):283-8.
1996: Jeltsch A; Wenz C; Wende W; Selent U; Pingoud A
Engineering novel restriction endonucleases: principles and applications.
Trends in biotechnology 1996;14(7):235-8.
1996: Friedhoff P; Kolmes B; Gimadutdinow O; Wende W; Krause K L; Pingoud A
Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.
Nucleic acids research 1996;24(14):2632-9.
1996: Stahl F; Wende W; Jeltsch A; Pingoud A
Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(12):6175-80.
1996: Jeltsch A; Sobotta T; Pingoud A
Structure prediction of the EcoRV DNA methyltransferase based on mutant profiling, secondary structure analysis, comparison with known structures of methyltransferases and isolation of catalytically inactive single mutants.
Protein engineering 1996;9(5):413-23.
1996: Wenz C; Jeltsch A; Pingoud A
Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone.
The Journal of biological chemistry 1996;271(10):5565-73.
1996: Jeltsch A; Pingoud A
Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems.
Journal of molecular evolution 1996;42(2):91-6.
1995: Meiss G; Friedhoff P; Hahn M; Gimadutdinow O; Pingoud A
Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease.
Biochemistry 1995;34(37):11979-88.
1995: Hahn M; Dörsam V; Friedhoff P; Fritz A; Pingoud A
Quantitative polymerase chain reaction with enzyme-linked immunosorbent assay detection of selectively digested amplified sample and control DNA.
Analytical biochemistry 1995;229(2):236-48.
1995: Jeltsch A; Kröger M; Pingoud A
Evidence for an evolutionary relationship among type-II restriction endonucleases.
Gene 1995;160(1):7-16.
1995: Jeltsch A; Maschke H; Selent U; Wenz C; Köhler E; Connolly B A; Thorogood H; Pingoud A
DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme.
Biochemistry 1995;34(18):6239-46.
1995: Jeltsch A; Pleckaityte M; Selent U; Wolfes H; Siksnys V; Pingoud A
Evidence for substrate-assisted catalysis in the DNA cleavage of several restriction endonucleases.
Gene 1995;157(1-2):157-62.
1995: Jeltsch A; Alves J; Urbanke C; Maass G; Eckstein H; Lianshan Z; Bayer E; Pingoud A
A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.
The Journal of biological chemistry 1995;270(10):5122-9.
1994: Wenz C; Selent U; Wende W; Jeltsch A; Wolfes H; Pingoud A
Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates.
Biochimica et biophysica acta 1994;1219(1):73-80.
1994: Friedhoff P; Gimadutdinow O; Pingoud A
Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis.
Nucleic acids research 1994;22(16):3280-7.
1994: Jeltsch A; Alves J; Wolfes H; Maass G; Pingoud A
Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.
Biochemistry 1994;33(34):10215-9.
1994: Friedhoff P; Gimadutdinow O; Rüter T; Wende W; Urbanke C; Thole H; Pingoud A
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli.
Protein expression and purification 1994;5(1):37-43.
1993: Friedhoff P; Hahn M; Wolfes H; Pingoud A
Quantitative polymerase chain reaction with oligodeoxynucleotide ligation assay/enzyme-linked immunosorbent assay detection.
Analytical biochemistry 1993;215(1):9-16.
1993: Jeltsch A; Alves J; Wolfes H; Maass G; Pingoud A
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(18):8499-503.
1993: Jeltsch A; Fritz A; Alves J; Wolfes H; Pingoud A
A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases.
Analytical biochemistry 1993;213(2):234-40.
1993: Stöver T; Köhler E; Fagin U; Wende W; Wolfes H; Pingoud A
Determination of the DNA bend angle induced by the restriction endonuclease EcoRV in the presence of Mg2+.
The Journal of biological chemistry 1993;268(12):8645-50.
1993: Jeltsch A; Alves J; Oelgeschläger T; Wolfes H; Maass G; Pingoud A
Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.
Journal of molecular biology 1993;229(1):221-34.
1992: Serth J; Weber W; Frech M; Wittinghofer A; Pingoud A
Binding of the H-ras p21 GTPase activating protein by the activated epidermal growth factor receptor leads to inhibition of the p21 GTPase activity in vitro.
Biochemistry 1992;31(28):6361-5.
1992: Jeltsch A; Alves J; Maass G; Pingoud A
On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling.
FEBS letters 1992;304(1):4-8.
1992: Selent U; Rüter T; Köhler E; Liedtke M; Thielking V; Alves J; Oelgeschläger T; Wolfes H; Peters F; Pingoud A
A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV.
Biochemistry 1992;31(20):4808-15.
1992: Thielking V; Selent U; Köhler E; Landgraf A; Wolfes H; Alves J; Pingoud A
Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease.
Biochemistry 1992;31(15):3727-32.
1991: Landgraf A; Reckmann B; Pingoud A
Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.
Analytical biochemistry 1991;198(1):86-91.
1991: Bensch K; Pieper U; Ott G; Schirmer N; Sprinzl M; Pingoud A
How many EF-Tu molecules participate in aminoacyl-tRNA binding?
Biochimie 1991;73(7-8):1045-50.
1991: Thielking V; Selent U; Köhler E; Wolfes H; Pieper U; Geiger R; Urbanke C; Winkler F K; Pingoud A
Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.
Biochemistry 1991;30(26):6416-22.
1991: Serth J; Lautwein A; Frech M; Wittinghofer A; Pingoud A
The inhibition of the GTPase activating protein-Ha-ras interaction by acidic lipids is due to physical association of the C-terminal domain of the GTPase activating protein with micellar structures.
The EMBO journal 1991;10(6):1325-30.
1991: Landgraf A; Reckmann B; Pingoud A
Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye.
Analytical biochemistry 1991;193(2):231-5.
1990: Pieper U; Ehbrecht H J; Fliess A; Schick B; Jurnak F; Pingoud A
Genetic engineering, isolation and characterization of a truncated Escherichia coli elongation factor Tu comprising domains 2 and 3.
Biochimica et biophysica acta 1990;1087(2):147-56.
1990: Pingoud A; Gast F U; Peters F
The influence of the concentrations of elongation factors and tRNAs on the dynamics and accuracy of protein biosynthesis.
Biochimica et biophysica acta 1990;1050(1-3):252-8.
1990: Thielking V; Alves J; Fliess A; Maass G; Pingoud A
Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences.
Biochemistry 1990;29(19):4682-91.
1990: Oelgeschläger T; Geiger R; Rüter T; Alves J; Fliess A; Pingoud A
Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.
Gene 1990;89(1):19-27.
1989: Alves J; Urbanke C; Fliess A; Maass G; Pingoud A
Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease.
Biochemistry 1989;28(19):7879-88.
1989: Alves J; Rüter T; Geiger R; Fliess A; Maass G; Pingoud A
Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
Biochemistry 1989;28(6):2678-84.
1989: Geiger R; Rüter T; Alves J; Fliess A; Wolfes H; Pingoud V; Urbanke C; Maass G; Pingoud A; Düsterhöft A
Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
Biochemistry 1989;28(6):2667-77.
1988: Pingoud A; Willumsen B M; Gallwitz D; Masters S B; Hershey J W
The guanine-nucleotide binding proteins. EMBO-NATO-CEC advanced research workshop organized by L. Bosch, B. Kraal and A. Parmeggiani in Renesse, The Netherlands, August 6-11, 1988.
FEBS letters 1988;242(1):1-3.
1988: Fliess A; Wolfes H; Seela F; Pingoud A
Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.
Nucleic acids research 1988;16(24):11781-93.
1988: Pingoud A; Wehrmann M; Pieper U; Gast F U; Urbanke C; Alves J; Feuerstein J; Wittinghofer A
Spectroscopic and hydrodynamic studies reveal structural differences in normal and transforming H-ras gene products.
Biochemistry 1988;27(13):4735-40.
1987: Gast F U; Peters F; Pingoud A
The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes.
The Journal of biological chemistry 1987;262(25):11920-6.
1986: Wolfes H; Alves J; Fliess A; Geiger R; Pingoud A
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.
Nucleic acids research 1986;14(22):9063-80.
1986: Wolfes H; Fliess A; Winkler F; Pingoud A
Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases.
European journal of biochemistry / FEBS 1986;159(2):267-73.
1986: Fliess A; Wolfes H; Rosenthal A; Schwellnus K; Blöcker H; Frank R; Pingoud A
Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.
Nucleic acids research 1986;14(8):3463-74.
1985: Wolfes H; Fliess A; Pingoud A
A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI.
European journal of biochemistry / FEBS 1985;150(1):105-10.
1985: Ehbrecht H J; Pingoud A; Urbanke C; Maass G; Gualerzi C
Linear diffusion of restriction endonucleases on DNA.
The Journal of biological chemistry 1985;260(10):6160-6.
1984: Pingoud A; Urbanke C; Alves J; Ehbrecht H J; Zabeau M; Gualerzi C
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases.
Biochemistry 1984;23(24):5697-703.
1983: Pingoud A; Gast F U; Block W; Peters F
The elongation factor Tu from Escherichia coli, aminoacyl-tRNA, and guanosine tetraphosphate form a ternary complex which is bound by programmed ribosomes.
The Journal of biological chemistry 1983;258(23):14200-5.

Sign-in to see more