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Thomas Pollard

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Thomas D Pollard; Enrique M De La Cruz
Take advantage of time in your experiments: a guide to simple, informative kinetics assays.
Thomas D Pollard
Political advocacy by the American Society for Cell Biology and its partners.
Thomas D Pollard
The obligation for biologists to commit to political advocacy.

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All Publications

2013: Thomas D Pollard; Enrique M De La Cruz
Take advantage of time in your experiments: a guide to simple, informative kinetics assays.
Molecular biology of the cell 2013;24(8):1103-10.
2012: Thomas D Pollard
Political advocacy by the American Society for Cell Biology and its partners.
Molecular biology of the cell 2012;23(21):4171-4.
2012: Thomas D Pollard
The obligation for biologists to commit to political advocacy.
Cell 2012;151(2):239-43.
2012: Thomas D Pollard; David R Burgess; Issei Mabuchi
Remembrance of Ray Rappaport, pioneer in the study of cytokinesis.
Cytoskeleton (Hoboken, N.J.) 2012;69(10):659-69.
2012: Shambaditya Saha; Thomas D Pollard
Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast.
Molecular biology of the cell 2012;23(20):3993-4007.
2012: Shambaditya Saha; Thomas D Pollard
Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast.
Molecular biology of the cell 2012;23(20):3982-92.
2012: Qing Yang; Xiao-Feng Zhang; Thomas D Pollard; Paul Forscher
Arp2/3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow.
The Journal of cell biology 2012;197(7):939-56.
2012: Jim Pfaendtner; Thomas D Pollard; Niels Volkmann; Paul Dalhaimer; Dorit Hanein; Gregory A Voth
Key structural features of the actin filament Arp2/3 complex branch junction revealed by molecular simulation.
Journal of molecular biology 2012;416(1):148-61.
2012: Qian Chen; Shalini Nag; Thomas D Pollard
Formins filter modified actin subunits during processive elongation.
Journal of structural biology 2012;177(1):32-9.
2011: Qian Chen; Thomas D Pollard
Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring.
The Journal of cell biology 2011;195(3):485-98.
2011: Rajesh Arasada; Thomas D Pollard
Distinct roles for F-BAR proteins Cdc15p and Bzz1p in actin polymerization at sites of endocytosis in fission yeast.
Current biology : CB 2011;21(17):1450-9.
2011: Shih-Chieh Ti; Christopher T Jurgenson; Bradley Nolen; Thomas D Pollard
Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(33):E463-71.
2011: Thomas D Pollard
Cell biology. Formin tip tracking.
Science (New York, N.Y.) 2011;331(6013):39-41.
2010: Thomas D Pollard
A guide to simple and informative binding assays.
Molecular biology of the cell 2010;21(23):4061-7.
2010: Paul Dalhaimer; Thomas D Pollard
Molecular dynamics simulations of Arp2/3 complex activation.
Biophysical journal 2010;99(8):2568-76.
2010: Thomas D Pollard; Jian-Qiu Wu
Understanding cytokinesis: lessons from fission yeast.
Nature reviews. Molecular cell biology 2010;11(2):149-55.
2010: Thomas D Pollard
Mechanics of cytokinesis in eukaryotes.
Current opinion in cell biology 2010;22(1):50-6.
2009: Thomas D Pollard; John A Cooper
Actin, a central player in cell shape and movement.
Science (New York, N.Y.) 2009;326(5957):1208-12.
2009: Bradley Nolen; N Tomasevic; A Russell; D W Pierce; Z Jia; C D McCormick; J Hartman; R Sakowicz; Thomas D Pollard
Characterization of two classes of small molecule inhibitors of Arp2/3 complex.
Nature 2009;460(7258):1031-4.
2009: Aditya S Paul; Thomas D Pollard
Review of the mechanism of processive actin filament elongation by formins.
Cell motility and the cytoskeleton 2009;66(8):606-17.
2009: Aditya S Paul; Thomas D Pollard
Energetic requirements for processive elongation of actin filaments by FH1FH2-formins.
The Journal of biological chemistry 2009;284(18):12533-40.
2009: Chikio Chan; Christopher C Beltzner; Thomas D Pollard
Cofilin dissociates Arp2/3 complex and branches from actin filaments.
Current biology : CB 2009;19(7):537-45.
2009: Thomas D Pollard; Julien Berro
Mathematical models and simulations of cellular processes based on actin filaments.
The Journal of biological chemistry 2009;284(9):5433-7.
2009: Obidimma C Ezezika; Noah S Younger; Jia Lu; Don Kaiser; Zachary A Corbin; Bradley Nolen; David R Kovar; Thomas D Pollard
Incompatibility with formin Cdc12p prevents human profilin from substituting for fission yeast profilin: insights from crystal structures of fission yeast profilin.
The Journal of biological chemistry 2009;284(4):2088-97.
2008: Bradley Nolen; Thomas D Pollard
Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
The Journal of biological chemistry 2008;283(39):26490-8.
2008: Rachel Mahaffy; Thomas D Pollard
Influence of phalloidin on the formation of actin filament branches by Arp2/3 complex.
Biochemistry 2008;47(24):6460-7.
2008: Matthew Lord; Thomas E Sladewski; Thomas D Pollard
Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(23):8014-9.
2008: Thomas D Pollard
Progress towards understanding the mechanism of cytokinesis in fission yeast.
Biochemical Society transactions 2008;36(Pt 3):425-30.
2008: Christopher C Beltzner; Thomas D Pollard
Pathway of actin filament branch formation by Arp2/3 complex.
The Journal of biological chemistry 2008;283(11):7135-44.
2008: Paul Dalhaimer; Thomas D Pollard; Bradley Nolen
Nucleotide-mediated conformational changes of monomeric actin and Arp3 studied by molecular dynamics simulations.
Journal of molecular biology 2008;376(1):166-83.
2008: Dimitrios Vavylonis; Jian-Qiu Wu; Steven Hao; Ben O'Shaughnessy; Thomas D Pollard
Assembly mechanism of the contractile ring for cytokinesis by fission yeast.
Science (New York, N.Y.) 2008;319(5859):97-100.
2007: Jeffrey R Kuhn; Thomas D Pollard
Single molecule kinetic analysis of actin filament capping. Polyphosphoinositides do not dissociate capping proteins.
The Journal of biological chemistry 2007;282(38):28014-24.
2007: Ikuko Fujiwara; Dimitrios Vavylonis; Thomas D Pollard
Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(21):8827-32.
2007: Bradley Nolen; Thomas D Pollard
Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex.
Molecular cell 2007;26(3):449-57.
2007: M Yusuf Ali; Elena B Krementsova; Guy G Kennedy; Rachel Mahaffy; Thomas D Pollard; Kathleen M Trybus; David M Warshaw
Myosin Va maneuvers through actin intersections and diffuses along microtubules.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(11):4332-6.
2007: Janna G Kiselar; Rachel Mahaffy; Thomas D Pollard; Steven C Almo; Mark R Chance
Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(5):1552-7.
2007: Thomas D Pollard
Regulation of actin filament assembly by Arp2/3 complex and formins.
Annual review of biophysics and biomolecular structure 2007;36():451-77.
2006: Rachel Mahaffy; Thomas D Pollard
Kinetics of the formation and dissociation of actin filament branches mediated by Arp2/3 complex.
Biophysical journal 2006;91(9):3519-28.
2006: Ernesto Andrianantoandro; Thomas D Pollard
Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin.
Molecular cell 2006;24(1):13-23.
2006: Jian-Qiu Wu; Vladimir Sirotkin; David R Kovar; Matthew Lord; Christopher C Beltzner; Jeffrey R Kuhn; Thomas D Pollard
Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast.
The Journal of cell biology 2006;174(3):391-402.
2006: Lior Haviv; Yifat Brill-Karniely; Rachel Mahaffy; Frederic Backouche; Avinoam Ben-Shaul; Thomas D Pollard; Anne Bernheim-Groswasser
Reconstitution of the transition from lamellipodium to filopodium in a membrane-free system.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(13):4906-11.
2006: Dimitrios Vavylonis; David R Kovar; Ben O'Shaughnessy; Thomas D Pollard
Model of formin-associated actin filament elongation.
Molecular cell 2006;21(4):455-66.
2006: David R Kovar; Elizabeth S Harris; Rachel Mahaffy; Henry N Higgs; Thomas D Pollard
Control of the assembly of ATP- and ADP-actin by formins and profilin.
Cell 2006;124(2):423-35.
2005: Matthew Lord; Ellen Laves; Thomas D Pollard
Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast.
Molecular biology of the cell 2005;16(11):5346-55.
2005: Jian-Qiu Wu; Thomas D Pollard
Counting cytokinesis proteins globally and locally in fission yeast.
Science (New York, N.Y.) 2005;310(5746):310-4.
2005: Vladimir Sirotkin; Christopher C Beltzner; Jean-Baptiste Marchand; Thomas D Pollard
Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
The Journal of cell biology 2005;170(4):637-48.
2005: David R Kovar; Jian-Qiu Wu; Thomas D Pollard
Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.
Molecular biology of the cell 2005;16(5):2313-24.
2005: Jeffrey R Kuhn; Thomas D Pollard
Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy.
Biophysical journal 2005;88(2):1387-402.
2005: Kirsi Turbedsky; Thomas D Pollard; Mark Yeager
Assembly of Acanthamoeba myosin-II minifilaments. Model of anti-parallel dimers based on EM and X-ray diffraction of 2D and 3D crystals.
Journal of molecular biology 2005;345(2):363-73.
2005: Kirsi Turbedsky; Thomas D Pollard
Assembly of Acanthamoeba myosin-II minifilaments. Definition of C-terminal residues required to form coiled-coils, dimers, and octamers.
Journal of molecular biology 2005;345(2):351-61.
2004: Bradley Nolen; Ryan S Littlefield; Thomas D Pollard
Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(44):15627-32.
2004: Matthew Lord; Thomas D Pollard
UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II.
The Journal of cell biology 2004;167(2):315-25.
2004: David R Kovar; Thomas D Pollard
Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(41):14725-30.
2004: Thomas D Pollard
Formins coming into focus.
Developmental cell 2004;6(3):312-4.
2004: Christopher C Beltzner; Thomas D Pollard
Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species.
Journal of molecular biology 2004;336(2):551-65.
2004: Dasantila Golemi-Kotra; Rachel Mahaffy; Matthew J Footer; Jennifer H Holtzman; Thomas D Pollard; Julie A Theriot; Alanna Schepartz
High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein.
Journal of the American Chemical Society 2004;126(1):4-5.
2004: Thomas D Pollard
Ray Rappaport chronology: Twenty-five years of seminal papers on cytokinesis in the Journal of Experimental Zoology.
Journal of experimental zoology. Part A, Comparative experimental biology 2004;301(1):9-14.
2003: Jian-Qiu Wu; Jeffrey R Kuhn; David R Kovar; Thomas D Pollard
Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.
Developmental cell 2003;5(5):723-34.
2003: David R Kovar; Jeffrey R Kuhn; Andrea L Tichy; Thomas D Pollard
The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin.
The Journal of cell biology 2003;161(5):875-87.
2003: Thomas D Pollard
The cytoskeleton, cellular motility and the reductionist agenda.
Nature 2003;422(6933):741-5.
2003: Thomas D Pollard; Gary G Borisy
Cellular motility driven by assembly and disassembly of actin filaments.
Cell 2003;112(4):453-65.
2003: Thomas D Pollard
Functional genomics of cell morphology using RNA interference: pick your style, broad or deep.
Journal of biology 2003;2(4):25.
2002: Hyun-Hee Kong; Thomas D Pollard
Intracellular localization and dynamics of myosin-II and myosin-IC in live Acanthamoeba by transient transfection of EGFP fusion proteins.
Journal of cell science 2002;115(Pt 24):4993-5002.
2002: Thomas D Pollard; Christopher C Beltzner
Structure and function of the Arp2/3 complex.
Current opinion in structural biology 2002;12(6):768-74.
2002: Laurent Blanchoin; Thomas D Pollard
Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin.
Biochemistry 2002;41(2):597-602.
2002: Thomas D Pollard
Cellular motility powered by actin filament assembly and disassembly.
Harvey lectures 2002;98():1-17.
2001: Kurt Amann; Thomas D Pollard
Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(26):15009-13.
2001: Robert Robinson; Kirsi Turbedsky; Don Kaiser; JB Marchand; HN Higgs; Senyon Choe; Thomas D Pollard
Crystal structure of Arp2/3 complex.
Science (New York, N.Y.) 2001;294(5547):1679-84.
2001: Ernesto Andrianantoandro; Laurent Blanchoin; D Sept; J A McCammon; Thomas D Pollard
Kinetic mechanism of end-to-end annealing of actin filaments.
Journal of molecular biology 2001;312(4):721-30.
2001: Laurent Blanchoin; Thomas D Pollard; S E Hitchcock-DeGregori
Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin.
Current biology : CB 2001;11(16):1300-4.
2001: Enrique M De La Cruz; Thomas D Pollard
Structural biology. Actin' up.
Science (New York, N.Y.) 2001;293(5530):616-8.
2001: Kurt Amann; Thomas D Pollard
The Arp2/3 complex nucleates actin filament branches from the sides of pre-existing filaments.
Nature cell biology 2001;3(3):306-10.
2001: Thomas D Pollard
Genomics, the cytoskeleton and motility.
Nature 2001;409(6822):842-3.
2001: HN Higgs; Thomas D Pollard
Regulation of actin filament network formation through ARP2/3 complex: activation by a diverse array of proteins.
Annual review of biochemistry 2001;70():649-76.
2001: JB Marchand; Don Kaiser; Thomas D Pollard; HN Higgs
Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex.
Nature cell biology 2001;3(1):76-82.
2001: Thomas D Pollard; Laurent Blanchoin; Dyche R D Mullins
Actin dynamics.
Journal of cell science 2001;114(Pt 1):3-4.
2000: Thomas D Pollard
Reflections on a quarter century of research on contractile systems.
Trends in biochemical sciences 2000;25(12):607-11.
2000: W L Lee; M Bezanilla; Thomas D Pollard
Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp.
The Journal of cell biology 2000;151(4):789-800.
2000: Y J Kim; Don Kaiser; Thomas D Pollard; Yoshitaka Ichikawa
Synthesis of (3R)-carboxy pyrrolidine (a beta-proline analogue) and its oligomer.
Bioorganic & medicinal chemistry letters 2000;10(21):2417-9.
2000: Laurent Blanchoin; Thomas D Pollard; Dyche R D Mullins
Interactions of ADF/cofilin, Arp2/3 complex, capping protein and profilin in remodeling of branched actin filament networks.
Current biology : CB 2000;10(20):1273-82.
2000: Kurt Amann; Thomas D Pollard
Cellular regulation of actin network assembly.
Current biology : CB 2000;10(20):R728-30.
2000: HN Higgs; Thomas D Pollard
Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex.
The Journal of cell biology 2000;150(6):1311-20.
2000: Laurent Blanchoin; Kurt Amann; HN Higgs; JB Marchand; Don Kaiser; Thomas D Pollard
Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins.
Nature 2000;404(6781):1007-11.
2000: M Bezanilla; J M Wilson; Thomas D Pollard
Fission yeast myosin-II isoforms assemble into contractile rings at distinct times during mitosis.
Current biology : CB 2000;10(7):397-400.
2000: E M De La Cruz; A Mandinova; Michel O Steinmetz; Daniel Stoffler; Ueli Aebi; Thomas D Pollard
Polymerization and structure of nucleotide-free actin filaments.
Journal of molecular biology 2000;295(3):517-26.
2000: Laurent Blanchoin; Robert Robinson; Senyon Choe; Thomas D Pollard
Phosphorylation of Acanthamoeba actophorin (ADF/cofilin) blocks interaction with actin without a change in atomic structure.
Journal of molecular biology 2000;295(2):203-11.
2000: Thomas D Pollard; Laurent Blanchoin; Dyche R D Mullins
Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
Annual review of biophysics and biomolecular structure 2000;29():545-76.
2000: M Bezanilla; Thomas D Pollard
Myosin-II tails confer unique functions in Schizosaccharomyces pombe: characterization of a novel myosin-II tail.
Molecular biology of the cell 2000;11(1):79-91.
1999: W L Lee; E M Ostap; H G Zot; Thomas D Pollard
Organization and ligand binding properties of the tail of Acanthamoeba myosin-IA. Identification of an actin-binding site in the basic (tail homology-1) domain.
The Journal of biological chemistry 1999;274(49):35159-71.
1999: HN Higgs; Laurent Blanchoin; Thomas D Pollard
Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization.
Biochemistry 1999;38(46):15212-22.
1999: HN Higgs; Thomas D Pollard
Regulation of actin polymerization by Arp2/3 complex and WASp/Scar proteins.
The Journal of biological chemistry 1999;274(46):32531-4.
1999: Don Kaiser; V K Vinson; Douglas B. Murphy; Thomas D Pollard
Profilin is predominantly associated with monomeric actin in Acanthamoeba.
Journal of cell science 1999;112 ( Pt 21)():3779-90.
1999: Robin C May; Maggie Hall; HN Higgs; Thomas D Pollard; Trinad Chakraborty; Jürgen Wehland; Laura M Machesky; Antonio S Sechi
The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes.
Current biology : CB 1999;9(14):759-62.
1999: Laurent Blanchoin; Thomas D Pollard
Mechanism of interaction of Acanthamoeba actophorin (ADF/Cofilin) with actin filaments.
The Journal of biological chemistry 1999;274(22):15538-46.
1999: Dyche R D Mullins; Thomas D Pollard
Rho-family GTPases require the Arp2/3 complex to stimulate actin polymerization in Acanthamoeba extracts.
Current biology : CB 1999;9(8):405-15.
1999: Dyche R D Mullins; Thomas D Pollard
Structure and function of the Arp2/3 complex.
Current opinion in structural biology 1999;9(2):244-9.
1999: Laura M Machesky; Dyche R D Mullins; HN Higgs; Don Kaiser; Laurent Blanchoin; Robin C May; Maggie Hall; Thomas D Pollard
Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(7):3739-44.
1998: Laurent Blanchoin; Thomas D Pollard
Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin.
The Journal of biological chemistry 1998;273(39):25106-11.
1998: S Liu; Alexander A Fedorov; Thomas D Pollard; Eaton E Lattman; Steven C Almo; K A Magnus
Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii.
Journal of structural biology 1998;123(1):22-9.
1998: V K Vinson; E M De La Cruz; H N Higgs; Thomas D Pollard
Interactions of Acanthamoeba profilin with actin and nucleotides bound to actin.
Biochemistry 1998;37(31):10871-80.
1998: Dyche R D Mullins; John Edward Heuser; Thomas D Pollard
The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(11):6181-6.
1998: J Xu; W H Schwarz; Josef Käs; Thomas P Stossel; Paul A Janmey; Thomas D Pollard
Mechanical properties of actin filament networks depend on preparation, polymerization conditions, and storage of actin monomers.
Biophysical journal 1998;74(5):2731-40.
1998: J Xu; D Wirtz; Thomas D Pollard
Dynamic cross-linking by alpha-actinin determines the mechanical properties of actin filament networks.
The Journal of biological chemistry 1998;273(16):9570-6.
1998: Dyche R D Mullins; J F Kelleher; J Xu; Thomas D Pollard
Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments.
Molecular biology of the cell 1998;9(4):841-52.
1997: M Bezanilla; Susan Forsburg; Thomas D Pollard
Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesis.
Molecular biology of the cell 1997;8(12):2693-705.
1997: S A Leonard; Apostolos G Gittis; E C Petrella; Thomas D Pollard; Eaton E Lattman
Crystal structure of the actin-binding protein actophorin from Acanthamoeba.
Nature structural biology 1997;4(5):369-73.
1997: Kirsi Turbedsky; Thomas D Pollard; Anne R Bresnick
A subset of protein kinase C phosphorylation sites on the myosin II regulatory light chain inhibits phosphorylation by myosin light chain kinase.
Biochemistry 1997;36(8):2063-7.
1997: R D Mullins; W F Stafford; Thomas D Pollard
Structure, subunit topology, and actin-binding activity of the Arp2/3 complex from Acanthamoeba.
The Journal of cell biology 1997;136(2):331-43.
1996: E C Petrella; L M Machesky; Don Kaiser; Thomas D Pollard
Structural requirements and thermodynamics of the interaction of proline peptides with profilin.
Biochemistry 1996;35(51):16535-43.
1996: E M De La Cruz; Thomas D Pollard
Kinetics and thermodynamics of phalloidin binding to actin filaments from three divergent species.
Biochemistry 1996;35(45):14054-61.
1996: Thomas D Pollard; E M Ostap
The chemical mechanism of myosin-I: implications for actin-based motility and the evolution of the myosin family of motor proteins.
Cell structure and function 1996;21(5):351-6.
1996: Dyche R D Mullins; J F Kelleher; Thomas D Pollard
Actin' like actin?
Trends in cell biology 1996;6(6):208-12.
1996: E M Ostap; Thomas D Pollard
Overlapping functions of myosin-I isoforms?
The Journal of cell biology 1996;133(2):221-4.
1996: E M Ostap; Thomas D Pollard
Biochemical kinetic characterization of the Acanthamoeba myosin-I ATPase.
The Journal of cell biology 1996;132(6):1053-60.
1995: Anne R Bresnick; V L Wolff-Long; O Baumann; Thomas D Pollard
Phosphorylation on threonine-18 of the regulatory light chain dissociates the ATPase and motor properties of smooth muscle myosin II.
Biochemistry 1995;34(39):12576-83.
1995: J F Kelleher; S J Atkinson; Thomas D Pollard
Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba.
The Journal of cell biology 1995;131(2):385-97.
1995: Thomas D Pollard
Actin cytoskeleton. Missing link for intracellular bacterial motility?
Current biology : CB 1995;5(8):837-40.
1995: S K Doberstein; GW Wiegand; L M Machesky; Thomas D Pollard
Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry.
Cytometry 1995;20(1):14-8.
1995: E M De La Cruz; Thomas D Pollard
Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics.
Biochemistry 1995;34(16):5452-61.
1994: E M De La Cruz; Thomas D Pollard
Transient kinetic analysis of rhodamine phalloidin binding to actin filaments.
Biochemistry 1994;33(48):14387-92.
1994: Trina A Schroer; EA Fyrberg; John A Cooper; R H Waterston; David M Helfman; Thomas D Pollard; D I Meyer
Actin-related protein nomenclature and classification.
The Journal of cell biology 1994;127(6 Pt 2):1777-8.
1994: P Maupin; C L Phillips; R S Adelstein; Thomas D Pollard
Differential localization of myosin-II isozymes in human cultured cells and blood cells.
Journal of cell science 1994;107 ( Pt 11)():3077-90.
1994: L M Machesky; S J Atkinson; Christophe Ampe; J Vandekerckhove; Thomas D Pollard
Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose.
The Journal of cell biology 1994;127(1):107-15.
1994: L M Machesky; N B Cole; Bernard Moss; Thomas D Pollard
Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin.
Biochemistry 1994;33(35):10815-24.
1994: D H Wachsstock; Thomas D Pollard
Transient state kinetics tutorial using the kinetics simulation program, KINSIM.
Biophysical journal 1994;67(3):1260-73.
1994: Alexander A Fedorov; K A Magnus; M H Graupe; Eaton E Lattman; Thomas D Pollard; Steven C Almo
X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(18):8636-40.
1994: Alexander A Fedorov; Thomas D Pollard; Steven C Almo
Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli.
Journal of molecular biology 1994;241(3):480-2.
1994: D H Wachsstock; W H Schwarz; Thomas D Pollard
Cross-linker dynamics determine the mechanical properties of actin gels.
Biophysical journal 1994;66(3 Pt 1):801-9.
1994: Steven C Almo; Thomas D Pollard; M Way; Eaton E Lattman
Purification, characterization and crystallization of Acanthamoeba profilin expressed in Escherichia coli.
Journal of molecular biology 1994;236(3):950-2.
1994: S J Archer; V K Vinson; Thomas D Pollard; Dennis A Torchia
Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy.
FEBS letters 1994;337(2):145-51.
1994: Thomas D Pollard; S Quirk
Profilins, ancient actin binding proteins with highly divergent primary structures.
Society of General Physiologists series 1994;49():117-28.
1994: Thomas D Pollard; Steven C Almo; S Quirk; V Vinson; Eaton E Lattman
Structure of actin binding proteins: insights about function at atomic resolution.
Annual review of cell biology 1994;10():207-49.
1993: JA Porter; M Yu; S K Doberstein; Thomas D Pollard; Craig Montell
Dependence of calmodulin localization in the retina on the NINAC unconventional myosin.
Science (New York, N.Y.) 1993;262(5136):1038-42.
1993: S K Doberstein; I C Baines; GW Wiegand; E D Korn; Thomas D Pollard
Inhibition of contractile vacuole function in vivo by antibodies against myosin-I.
Nature 1993;365(6449):841-3.
1993: V K Vinson; S J Archer; Eaton E Lattman; Thomas D Pollard; Dennis A Torchia
Three-dimensional solution structure of Acanthamoeba profilin-I.
The Journal of cell biology 1993;122(6):1277-83.
1993: S J Archer; V K Vinson; Thomas D Pollard; Dennis A Torchia
Secondary structure and topology of Acanthamoeba profilin I as determined by heteronuclear nuclear magnetic resonance spectroscopy.
Biochemistry 1993;32(26):6680-7.
1993: D H Wachsstock; W H Schwartz; Thomas D Pollard
Affinity of alpha-actinin for actin determines the structure and mechanical properties of actin filament gels.
Biophysical journal 1993;65(1):205-14.
1993: Thomas D Pollard; D Bhandari; P Maupin; D Wachsstock; A G Weeds; H G Zot
Direct visualization by electron microscopy of the weakly bound intermediates in the actomyosin adenosine triphosphatase cycle.
Biophysical journal 1993;64(2):454-71.
1992: Thomas D Pollard
Graduate training in cellular biophysics.
Biophysical journal 1992;63(5):1450-4.
1992: Thomas D Pollard; I Goldberg; W H Schwarz
Nucleotide exchange, structure, and mechanical properties of filaments assembled from ATP-actin and ADP-actin.
The Journal of biological chemistry 1992;267(28):20339-45.
1992: Pascal J Goldschmidt-Clermont; M I Furman; D Wachsstock; Daniel Safer; VT Nachmias; Thomas D Pollard
The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells.
Molecular biology of the cell 1992;3(9):1015-24.
1992: L L Satterwhite; M J Lohka; Katherine L Wilson; T Y Scherson; L J Cisek; Jeffry L Corden; Thomas D Pollard
Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis.
The Journal of cell biology 1992;118(3):595-605.
1992: S Yonemura; Thomas D Pollard
The localization of myosin I and myosin II in Acanthamoeba by fluorescence microscopy.
Journal of cell science 1992;102 ( Pt 3)():629-42.
1992: S J Atkinson; S K Doberstein; Thomas D Pollard
Moving off the beaten track.
Current biology : CB 1992;2(6):326-8.
1992: S K Doberstein; Thomas D Pollard
Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC.
The Journal of cell biology 1992;117(6):1241-9.
1992: L L Satterwhite; Thomas D Pollard
Cytokinesis.
Current opinion in cell biology 1992;4(1):43-52.
1992: H G Zot; S K Doberstein; Thomas D Pollard
Myosin-I moves actin filaments on a phospholipid substrate: implications for membrane targeting.
The Journal of cell biology 1992;116(2):367-76.
1991: S K Maciver; D H Wachsstock; W H Schwarz; Thomas D Pollard
The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin.
The Journal of cell biology 1991;115(6):1621-8.
1991: S K Maciver; H G Zot; Thomas D Pollard
Characterization of actin filament severing by actophorin from Acanthamoeba castellanii.
The Journal of cell biology 1991;115(6):1611-20.
1991: Pascal J Goldschmidt-Clermont; L M Machesky; S K Doberstein; Thomas D Pollard
Mechanism of the interaction of human platelet profilin with actin.
The Journal of cell biology 1991;113(5):1081-9.
1991: Pascal J Goldschmidt-Clermont; J W Kim; L M Machesky; S G Rhee; Thomas D Pollard
Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation.
Science (New York, N.Y.) 1991;251(4998):1231-3.
1991: P Sampath; Thomas D Pollard
Effects of cytochalasin, phalloidin, and pH on the elongation of actin filaments.
Biochemistry 1991;30(7):1973-80.
1991: Thomas D Pollard; S K Doberstein; H G Zot
Myosin-I.
Annual review of physiology 1991;53():653-81.
1991: Thomas D Pollard; David L Rimm
Analysis of cDNA clones for Acanthamoeba profilin-I and profilin-II shows end to end homology with vertebrate profilins and a small family of profilin genes.
Cell motility and the cytoskeleton 1991;20(2):169-77.
1990: John H Sinard; David L Rimm; Thomas D Pollard
Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. II. Assembly properties of tails with NH2- and COOH-terminal deletions.
The Journal of cell biology 1990;111(6 Pt 1):2417-26.
1990: David L Rimm; Don Kaiser; D Bhandari; P Maupin; D P Kiehart; Thomas D Pollard
Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.
The Journal of cell biology 1990;111(6 Pt 1):2405-16.
1990: Thomas D Pollard; P Maupin; John H Sinard; H E Huxley
A stopped-flow/rapid-freezing machine with millisecond time resolution to prepare intermediates in biochemical reactions for electron microscopy.
Journal of electron microscopy technique 1990;16(2):160-6.
1990: Pascal J Goldschmidt-Clermont; L M Machesky; Joseph J Baldassare; Thomas D Pollard
The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C.
Science (New York, N.Y.) 1990;247(4950):1575-8.
1990: John H Sinard; Thomas D Pollard
Acanthamoeba myosin-II minifilaments assemble on a millisecond time scale with rate constants greater than those expected for a diffusion limited reaction.
The Journal of biological chemistry 1990;265(7):3654-60.
1990: Thomas D Pollard
Actin.
Current opinion in cell biology 1990;2(1):33-40.
1989: John H Sinard; W F Stafford; Thomas D Pollard
The mechanism of assembly of Acanthamoeba myosin-II minifilaments: minifilaments assemble by three successive dimerization steps.
The Journal of cell biology 1989;109(4 Pt 1):1537-47.
1989: John H Sinard; Thomas D Pollard
The effect of heavy chain phosphorylation and solution conditions on the assembly of Acanthamoeba myosin-II.
The Journal of cell biology 1989;109(4 Pt 1):1529-35.
1989: Robert J Adams; Thomas D Pollard
Binding of myosin I to membrane lipids.
Nature 1989;340(6234):565-8.
1989: David L Rimm; Thomas D Pollard
Purification and characterization of an Acanthamoeba nuclear actin-binding protein.
The Journal of cell biology 1989;109(2):585-91.
1989: David L Rimm; John H Sinard; Thomas D Pollard
Location of the head-tail junction of myosin.
The Journal of cell biology 1989;108(5):1783-9.
1989: David L Rimm; Thomas D Pollard
New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli.
Gene 1989;75(2):323-7.
1989: Robert J Adams; Thomas D Pollard
Membrane-bound myosin-I provides new mechanisms in cell motility.
Cell motility and the cytoskeleton 1989;14(2):178-82.
1989: Don Kaiser; Pascal J Goldschmidt-Clermont; B A Levine; Thomas D Pollard
Characterization of renatured profilin purified by urea elution from poly-L-proline agarose columns.
Cell motility and the cytoskeleton 1989;14(2):251-62.
1989: John H Sinard; Thomas D Pollard
Microinjection into Acanthamoeba castellanii of monoclonal antibodies to myosin-II slows but does not stop cell locomotion.
Cell motility and the cytoskeleton 1989;12(1):42-52.
1988: K A Magnus; S K Maciver; Thomas D Pollard
Crystallization of actophorin, an actin filament-severing protein from Acanthamoeba.
The Journal of biological chemistry 1988;263(34):18143-4.
1988: David L Rimm; Thomas D Pollard; P Hieter
Resolution of Acanthamoeba castellanii chromosomes by pulsed field gel electrophoresis and construction of the initial linkage map.
Chromosoma 1988;97(3):219-23.
1988: Douglas B. Murphy; R O Gray; W A Grasser; Thomas D Pollard
Direct demonstration of actin filament annealing in vitro.
The Journal of cell biology 1988;106(6):1947-54.
1988: M Sato; W H Schwartz; S C Selden; Thomas D Pollard
Mechanical properties of brain tubulin and microtubules.
The Journal of cell biology 1988;106(4):1205-11.
1988: S Lee; Min Li; Thomas D Pollard
Evaluation of the binding of Acanthamoeba profilin to pyrene-labeled actin by fluorescence enhancement.
Analytical biochemistry 1988;168(1):148-55.
1986: S J Hagen; D P Kiehart; Don Kaiser; Thomas D Pollard
Characterization of monoclonal antibodies to Acanthamoeba myosin-I that cross-react with both myosin-II and low molecular mass nuclear proteins.
The Journal of cell biology 1986;103(6 Pt 1):2121-8.
1986: K A Magnus; Eaton E Lattman; M Sato; Thomas D Pollard
Crystallization of Acanthamoeba profilin-I.
The Journal of biological chemistry 1986;261(28):13360-1.
1986: D P Kiehart; Don Kaiser; Thomas D Pollard
Antibody inhibitors of nonmuscle myosin function and assembly.
Methods in enzymology 1986;134():423-53.
1986: Thomas D Pollard; P C Tseng; D L Rimm; D P Bichell; R C Williams; John H Sinard; M Sato
Characterization of alpha-actinin from Acanthamoeba.
Cell motility and the cytoskeleton 1986;6(6):649-61.
1984: D P Kiehart; Don Kaiser; Thomas D Pollard
Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail.
The Journal of cell biology 1984;99(3):1015-23.
1984: D P Kiehart; Don Kaiser; Thomas D Pollard
Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba.
The Journal of cell biology 1984;99(3):1002-14.