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Loredano Pollegioni

Research Profile

Chemicals & Drugs

Living Beings

Concepts & Ideas

Phenomena

Physiology

Mutagenesis, Site-Directed

Genes & Molecular Sequences

Amino Acid Sequences

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Publications

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Seth C Hopkins; Steven W Jones; Patrick Koch; Michael S Allen; Carrie A Bowen; Milan Chytil; Q Kevin Fang; Robert J Foglesong; Larry W Hardy; Michele L R Heffernan; Laurence Melnick; Gianluca Molla; Marco Nardini; Stephane H R Oliet; Michael A Orsini; Aude Panatier; Luciano Piubelli; Loredano Pollegioni; Lakshmi D Saraswat; Mustapha Soukri; Kerry L Spear; Mark A Varney; Thomas H Large
Structural, Kinetic, and Pharmacodynamic Mechanisms of d-Amino Acid Oxidase Inhibition by Small Molecules.
Yan Li; Loredano Pollegioni; Silvia Sacchi; Alo C Basu; Joseph T Coyle; Vadim Y Bolshakov
Identity of endogenous NMDAR glycine site agonist in amygdala is determined by synaptic activity level.
Emily Golden; Gianluca Molla; Rachel Paterson; Loredano Pollegioni; Elena Rosini; Wan Jun Tie; Anandhi Anandan; Gavin Flematti; Alice Vrielink
Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.

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All Publications

2013: Seth C Hopkins; Steven W Jones; Patrick Koch; Michael S Allen; Carrie A Bowen; Milan Chytil; Q Kevin Fang; Robert J Foglesong; Larry W Hardy; Michele L R Heffernan; Laurence Melnick; Gianluca Molla; Marco Nardini; Stephane H R Oliet; Michael A Orsini; Aude Panatier; Luciano Piubelli; Loredano Pollegioni; Lakshmi D Saraswat; Mustapha Soukri; Kerry L Spear; Mark A Varney; Thomas H Large
Structural, Kinetic, and Pharmacodynamic Mechanisms of d-Amino Acid Oxidase Inhibition by Small Molecules.
Journal of medicinal chemistry 2013;56(9):3710-24.
2013: Yan Li; Loredano Pollegioni; Silvia Sacchi; Alo C Basu; Joseph T Coyle; Vadim Y Bolshakov
Identity of endogenous NMDAR glycine site agonist in amygdala is determined by synaptic activity level.
Nature communications 2013;4():1760.
2013: Emily Golden; Gianluca Molla; Rachel Paterson; Loredano Pollegioni; Elena Rosini; Wan Jun Tie; Anandhi Anandan; Gavin Flematti; Alice Vrielink
Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.
The Biochemical journal 2013;451(2):217-26.
2013: Livia Curcio; Pamela Cappelletti; Claudio Grassi; Lucia Leone; Alessia Mastrodonato; Roberto Piacentini; Maria V Podda; Loredano Pollegioni; Silvia Sacchi; Marcello D'Ascenzo
Reduced D-serine levels in the nucleus accumbens of cocaine-treated rats hinder the induction of NMDA receptor-dependent synaptic plasticity.
Brain : a journal of neurology 2013;136(Pt 4):1216-30.
2013: Loredano Pollegioni; Elena Rosini; Gianluca Molla
Cephalosporin C acylase: dream and(/or) reality.
Applied microbiology and biotechnology 2013;97(6):2341-55.
2013: Laura Caldinelli; Gianluca Molla; Marco Nardini; Silvia Sacchi; Loredano Pollegioni
Characterization of human DAAO variants potentially related to an increased risk of schizophrenia.
Biochimica et biophysica acta 2013;1832(3):400-10.
2013: Laura Caldinelli; Diego Albani; Loredano Pollegioni
One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli.
BMC biotechnology 2013;13():32.
2013: Elisa Binda; Giorgia Letizia Marcone; Francesca Berini; Loredano Pollegioni; Flavia Marinelli
Streptomyces spp. as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance.
BMC biotechnology 2013;13():24.
2013: Silvia Sacchi; Loredano Pollegioni; Elena Rosini; Gianluca Molla
D-amino acid oxidase inhibitors as a novel class of drugs for schizophrenia therapy.
Current pharmaceutical design 2013;19(14):2499-511.
2012: Silvia Sacchi; Loredano Pollegioni; Laura Caldinelli; Pamela Cappelletti; Gianluca Molla
Structure-function relationships in human D-amino acid oxidase.
Amino acids 2012;43(5):1833-50.
2012: Elisa Binda; Giorgia Letizia Marcone; Loredano Pollegioni; Flavia Marinelli
Characterization of VanYn, a novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance in Nonomuraea sp. ATCC 39727.
The FEBS journal 2012;279(17):3203-13.
2012: Thomas Papouin; Loredano Pollegioni; Jérôme Ruel; Silvia Sacchi; Laurent Groc; Marwa Hanini; Marilyne Labasque; Laurent Ladépêche; Jean-Pierre Mothet; Stéphane H R Oliet
Synaptic and extrasynaptic NMDA receptors are gated by different endogenous coagonists.
Cell 2012;150(3):633-46.
2012: Simona Esposito; Armando Felsani; Fulvio Florenzano; Luigi Manni; Giovanna Maresca; Loredano Pollegioni; Andrea Pristerà; Borsello Tiziana; Sebastiano Cavallaro; Maria T Ciotti; Nadia Canu
Contribution of serine racemase/d-serine pathway to neuronal apoptosis.
Aging cell 2012;11(4):588-98.
2012: Pierre Pernot; Loredano Pollegioni; Yannick Tholance; Natalia Vasylieva; Raymond Cespuglio; Gabriel Debilly; Caroline Maucler; Stéphane Marinesco
d-Serine diffusion through the blood-brain barrier: effect on d-serine compartmentalization and storage.
Neurochemistry international 2012;60(8):837-45.
2012: Elena Tallarita; Loredano Pollegioni; Stefano Servi; Gianluca Molla
Expression in Escherichia coli of the catalytic domain of human proline oxidase.
Protein expression and purification 2012;82(2):345-51.
2012: Pascal Fossat; Mark J Millan; Jérôme Dulong; Stéphane H R Oliet; Loredano Pollegioni; Jean-Michel Rivet; Silvia Sacchi; Ting Shi; Jonathan V Sweedler; Fabrice R Turpin; Jean-Pierre Mothet
Glial D-serine gates NMDA receptors at excitatory synapses in prefrontal cortex.
Cerebral cortex (New York, N.Y. : 1991) 2012;22(3):595-606.
2012: Gabriella Tedeschi; Loredano Pollegioni; Armando Negri
Assays of D-amino acid oxidases.
Methods in molecular biology (Clifton, N.J.) 2012;794():381-95.
2012: Silvia Sacchi; Elena Rosini; Laura Caldinelli; Loredano Pollegioni
Biosensors for D-amino acid detection.
Methods in molecular biology (Clifton, N.J.) 2012;794():313-24.
2011: Federica Volontè; Stefano Servi; Fiorenza Viani; Paola D'Arrigo; Gianluca Molla; Ines Pisanelli; Loredano Pollegioni
Overexpression of a bacterial chymotrypsin: application for L-amino acid ester hydrolysis.
Enzyme and microbial technology 2011;49(6-7):560-6.
2011: Sandro Ghisla; Loredano Pollegioni; Gianluca Molla
Revisitation of the βCl-elimination reaction of D-amino acid oxidase: new interpretation of the reaction that sparked flavoprotein dehydrogenation mechanisms.
The Journal of biological chemistry 2011;286(47):40987-98.
2011: Luca F Frattini; Gianluca Molla; Luciano Piubelli; Silvia Sacchi; Loredano Pollegioni
Is rat an appropriate animal model to study the involvement of D-serine catabolism in schizophrenia? Insights from characterization of D-amino acid oxidase.
The FEBS journal 2011;278(22):4362-73.
2011: Luca Frattini; Loredano Pollegioni; Elena Rosini; Mirella S Pilone
Analyzing the D-amino acid content in biological samples by engineered enzymes.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2011;879(29):3235-9.
2011: Silvia Sacchi; Pamela Cappelletti; Stefano Giovannardi; Loredano Pollegioni
Evidence for the interaction of D-amino acid oxidase with pLG72 in a glial cell line.
Molecular and cellular neurosciences 2011;48(1):20-8.
2011: Loredano Pollegioni; Ernst Schonbrunn; Daniel Siehl
Molecular basis of glyphosate resistance-different approaches through protein engineering.
The FEBS journal 2011;278(16):2753-66.
2011: Natalia Vasylieva; Bogdan Barnych; Anne Meiller; Caroline Maucler; Loredano Pollegioni; Jian-Sheng Lin; Daniel Barbier; Stéphane Marinesco
Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation.
Biosensors & bioelectronics 2011;26(10):3993-4000.
2011: Loredano Pollegioni; Gianluca Molla
New biotech applications from evolved D-amino acid oxidases.
Trends in biotechnology 2011;29(6):276-83.
2011: Elena Rosini; Gianluca Molla; Sandro Ghisla; Loredano Pollegioni
On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity.
The FEBS journal 2011;278(3):482-92.
2011: Federica Volontè; Luciano Piubelli; Loredano Pollegioni
Optimizing HIV-1 protease production in Escherichia coli as fusion protein.
Microbial cell factories 2011;10():53.
2010: Jan Saam; Elena Rosini; Gianluca Molla; Klaus Schulten; Loredano Pollegioni; Sandro Ghisla
O2 reactivity of flavoproteins: dynamic access of dioxygen to the active site and role of a H+ relay system in D-amino acid oxidase.
The Journal of biological chemistry 2010;285(32):24439-46.
2010: Laura Caldinelli; Gianluca Molla; Luisa Bracci; Barbara Lelli; Silvia Pileri; Pamela Cappelletti; Silvia Sacchi; Loredano Pollegioni
Effect of ligand binding on human D-amino acid oxidase: implications for the development of new drugs for schizophrenia treatment.
Protein science : a publication of the Protein Society 2010;19(8):1500-12.
2010: Loredano Pollegioni; Silvia Sacchi
Metabolism of the neuromodulator D-serine.
Cellular and molecular life sciences : CMLS 2010;67(14):2387-404.
2010: Federica Volontè; Loredano Pollegioni; Gianluca Molla; Luca Frattini; Flavia Marinelli; Luciano Piubelli
Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli.
BMC biotechnology 2010;10():33.
2009: Mattia Pedotti; Elena Rosini; Gianluca Molla; Tommaso Moschetti; Carmelinda Savino; beatrice vallone; Loredano Pollegioni
Glyphosate resistance by engineering the flavoenzyme glycine oxidase.
The Journal of biological chemistry 2009;284(52):36415-23.
2009: Loredano Pollegioni
Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool.
The FEBS journal 2009;276(23):6825.
2009: Loredano Pollegioni; Luciano Piubelli; Gianluca Molla
Cholesterol oxidase: biotechnological applications.
The FEBS journal 2009;276(23):6857-70.
2009: Laura Caldinelli; Mattia Pedotti; Laura Motteran; Gianluca Molla; Loredano Pollegioni
FAD binding in glycine oxidase from Bacillus subtilis.
Biochimie 2009;91(11-12):1499-508.
2009: Diego Romano; Gianluca Molla; Loredano Pollegioni; Flavia Marinelli
Optimization of human D-amino acid oxidase expression in Escherichia coli.
Protein expression and purification 2009;68(1):72-8.
2009: Elena Rosini; Loredano Pollegioni; Sandro Ghisla; Roberto Orru; Gianluca Molla
Optimization of D-amino acid oxidase for low substrate concentrations--towards a cancer enzyme therapy.
The FEBS journal 2009;276(17):4921-32.
2009: Stefano Donini; Manuela Ferrari; Chiara Fedeli; Marco Faini; Ilaria Lamberto; Ada Serena Marletta; Lara Mellini; Michela Panini; Riccardo Percudani; Loredano Pollegioni; Laura Caldinelli; Stefania Petrucco; Alessio Peracchi
Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism.
The Biochemical journal 2009;422(2):265-72.
2009: Mattia Pedotti; Sandro Ghisla; Laura Motteran; Gianluca Molla; Loredano Pollegioni
Catalytic and redox properties of glycine oxidase from Bacillus subtilis.
Biochimie 2009;91(5):604-12.
2009: Laura Caldinelli; Gianluca Molla; Silvia Sacchi; Mirella S Pilone; Loredano Pollegioni
Relevance of weak flavin binding in human D-amino acid oxidase.
Protein science : a publication of the Protein Society 2009;18(4):801-10.
2008: Federica Volontè; Flavia Marinelli; Luciano Gastaldo; Silvia Sacchi; Mirella S Pilone; Loredano Pollegioni; Gianluca Molla
Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli.
Protein expression and purification 2008;61(2):131-7.
2008: Luciano Piubelli; Mattia Pedotti; Gianluca Molla; Susanne Feindler-Boeckh; Sandro Ghisla; Mirella S Pilone; Loredano Pollegioni
On the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in brevibacterium sterolicum cholesterol oxidase.
The Journal of biological chemistry 2008;283(36):24738-47.
2008: Silvia Sacchi; Mariagrazia Bernasconi; Magalie Martineau; Jean-Pierre Mothet; Maria Ruzzene; Mirella S Pilone; Loredano Pollegioni; Gianluca Molla
pLG72 modulates intracellular D-serine levels through its interaction with D-amino acid oxidase: effect on schizophrenia susceptibility.
The Journal of biological chemistry 2008;283(32):22244-56.
2008: Elena Rosini; Gianluca Molla; Carlo Rossetti; Mirella S Pilone; Loredano Pollegioni; Silvia Sacchi
A biosensor for all D-amino acids using evolved D-amino acid oxidase.
Journal of biotechnology 2008;135(4):377-84.
2008: Pierre Pernot; Jean-Pierre Mothet; Oleg Schuvailo; Alexey Soldatkin; Loredano Pollegioni; Mirella Pilone; Marie-Thérèse Adeline; Raymond Cespuglio; Stéphane Marinesco
Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system.
Analytical chemistry 2008;80(5):1589-97.
2008: Laura Caldinelli; Stefania Iametti; Alberto Barbiroli; Dimitrios Fessas; Francesco Bonomi; Luciano Piubelli; Gianluca Molla; Loredano Pollegioni
Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD.
Protein science : a publication of the Protein Society 2008;17(3):409-19.
2008: Loredano Pollegioni; Gianluca Molla; Silvia Sacchi; Elena Rosini; Roberto Verga; Mirella S Pilone
Properties and applications of microbial D-amino acid oxidases: current state and perspectives.
Applied microbiology and biotechnology 2008;78(1):1-16.
2007: Christopher W M Kay; Hassane El-Mkami; Gianluca Molla; Loredano Pollegioni; Rona R Ramsay
Characterization of the covalently bound anionic flavin radical in monoamine oxidase a by electron paramagnetic resonance.
Journal of the American Chemical Society 2007;129(51):16091-7.
2007: Loredano Pollegioni; Silvia Sacchi; Laura Caldinelli; Angelo Boselli; Mirella S Pilone; Luciano Piubelli; Gianluca Molla
Engineering the properties of D-amino acid oxidases by a rational and a directed evolution approach.
Current protein & peptide science 2007;8(6):600-18.
2007: Angelo Boselli; Elena Rosini; Giorgia Letizia Marcone; Silvia Sacchi; Laura Motteran; Mirella S Pilone; Loredano Pollegioni; Gianluca Molla
Glycine oxidase from Bacillus subtilis: role of histidine 244 and methionine 261.
Biochimie 2007;89(11):1372-80.
2007: Loredano Pollegioni; Luciano Piubelli; Silvia Sacchi; Mirella S Pilone; Gianluca Molla
Physiological functions of D-amino acid oxidases: from yeast to humans.
Cellular and molecular life sciences : CMLS 2007;64(11):1373-94.
2007: Angelo Boselli; Luciano Piubelli; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni; Silvia Sacchi
Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity.
Biochimie 2007;89(3):360-8.
2006: Louis Lim; Gianluca Molla; Nicole Guinn; Sandro Ghisla; Loredano Pollegioni; Alice Vrielink
Structural and kinetic analyses of the H121A mutant of cholesterol oxidase.
The Biochemical journal 2006;400(1):13-22.
2006: Aude Panatier; Dionysia T Theodosis; Jean-Pierre Mothet; Bastien Touquet; Loredano Pollegioni; Dominique A Poulain; Stéphane H R Oliet
Glia-derived D-serine controls NMDA receptor activity and synaptic memory.
Cell 2006;125(4):775-84.
2006: Gianluca Molla; Mariagrazia Bernasconi; Silvia Sacchi; Mirella S Pilone; Loredano Pollegioni
Expression in Escherichia coli and in vitro refolding of the human protein pLG72.
Protein expression and purification 2006;46(1):150-5.
2006: Laura Caldinelli; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis.
The FEBS journal 2006;273(3):504-12.
2005: Loredano Pollegioni; Simona Lorenzi; Elena Rosini; Giorgia Letizia Marcone; Gianluca Molla; Roberto Verga; Walter Cabri; Mirella S Pilone
Evolution of an acylase active on cephalosporin C.
Protein science : a publication of the Protein Society 2005;14(12):3064-76.
2005: Laura Caldinelli; Stefania Iametti; Alberto Barbiroli; Francesco Bonomi; Dimitrios Fessas; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin.
The Journal of biological chemistry 2005;280(24):22572-81.
2005: Jean-Pierre Mothet; Loredano Pollegioni; Gilles Ouanounou; Magalie Martineau; Philippe Fossier; Gérard Baux
Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(15):5606-11.
2004: Angelo Boselli; Luciano Piubelli; Gianluca Molla; Silvia Sacchi; Mirella S Pilone; Sandro Ghisla; Loredano Pollegioni
On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335.
Biochimica et biophysica acta 2004;1702(1):19-32.
2004: Mario Mörtl; Kay Diederichs; Wolfram Welte; Gianluca Molla; Laura Motteran; Gabriella Andriolo; Mirella S Pilone; Loredano Pollegioni
Structure-function correlation in glycine oxidase from Bacillus subtilis.
The Journal of biological chemistry 2004;279(28):29718-27.
2004: Laura Caldinelli; Stefania Iametti; Alberto Barbiroli; Francesco Bonomi; Luciano Piubelli; Pasquale Ferranti; Gianluca Picariello; Mirella S Pilone; Loredano Pollegioni
Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase.
The Journal of biological chemistry 2004;279(27):28426-34.
2004: Silvia Sacchi; Elena Rosini; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Modulating D-amino acid oxidase substrate specificity: production of an enzyme for analytical determination of all D-amino acids by directed evolution.
Protein engineering, design & selection : PEDS 2004;17(6):517-25.
2004: Loredano Pollegioni; Laura Caldinelli; Gianluca Molla; Silvia Sacchi; Mirella S Pilone
Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources.
Biotechnology progress 2004;20(2):467-73.
2003: Luciano Piubelli; Gianluca Molla; Laura Caldinelli; Mirella S Pilone; Loredano Pollegioni
Dissection of the structural determinants involved in formation of the dimeric form of D-amino acid oxidase from Rhodotorula gracilis: role of the size of the betaF5-betaF6 loop.
Protein engineering 2003;16(12):1063-9.
2003: Gianluca Molla; Laura Motteran; Luciano Piubelli; Mirella S Pilone; Loredano Pollegioni
Regulation of D-amino acid oxidase expression in the yeast Rhodotorula gracilis.
Yeast (Chichester, England) 2003;20(12):1061-9.
2003: Loredano Pollegioni; Stefania Iametti; Dimitrios Fessas; Laura Caldinelli; Luciano Piubelli; Alberto Barbiroli; Mirella S Pilone; Francesco Bonomi
Contribution of the dimeric state to the thermal stability of the flavoprotein D-amino acid oxidase.
Protein science : a publication of the Protein Society 2003;12(5):1018-29.
2003: Gianluca Molla; Laura Motteran; Viviana Job; Mirella S Pilone; Loredano Pollegioni
Kinetic mechanisms of glycine oxidase from Bacillus subtilis.
European journal of biochemistry / FEBS 2003;270(7):1474-82.
2002: Loredano Pollegioni; Kay Diederichs; Gianluca Molla; Stephan Umhau; Wolfram Welte; Sandro Ghisla; Mirella S Pilone
Yeast D-amino acid oxidase: structural basis of its catalytic properties.
Journal of molecular biology 2002;324(3):535-46.
2002: Angelo Boselli; Silvia Sacchi; Viviana Job; Mirella S Pilone; Loredano Pollegioni
Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study.
European journal of biochemistry / FEBS 2002;269(19):4762-71.
2002: Luciano Piubelli; Laura Caldinelli; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Conversion of the dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A rational mutagenesis approach.
FEBS letters 2002;526(1-3):43-8.
2002: Silvia Sacchi; Simona Lorenzi; Gianluca Molla; Mirella S Pilone; Carlo Rossetti; Loredano Pollegioni
Engineering the substrate specificity of D-amino-acid oxidase.
The Journal of biological chemistry 2002;277(30):27510-6.
2002: Elena Ardini; Barbara Sporchia; Loredano Pollegioni; Michele Modugno; Cristina Ghirelli; Fabio Castiglioni; Elda Tagliabue; Sylvie Ménard
Identification of a novel function for 67-kDa laminin receptor: increase in laminin degradation rate and release of motility fragments.
Cancer research 2002;62(5):1321-5.
2002: Viviana Job; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli.
European journal of biochemistry / FEBS 2002;269(5):1456-63.
2002: Viviana Job; Giorgia Letizia Marcone; Mirella S Pilone; Loredano Pollegioni
Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein.
The Journal of biological chemistry 2002;277(9):6985-93.
2002: Davide Vigetti; Loredano Pollegioni; Claudio Monetti; Mariangela Prati; Giovanni Bernardini; Rosalba Gornati
Property comparison of recombinant amphibian and mammalian allantoicases.
FEBS letters 2002;512(1-3):323-8.
2001: Loredano Pollegioni; CM Harris; Gianluca Molla; Mirella S Pilone; Sandro Ghisla
Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase.
FEBS letters 2001;507(3):323-6.
2001: CM Harris; Loredano Pollegioni; Sandro Ghisla
pH and kinetic isotope effects in d-amino acid oxidase catalysis.
European journal of biochemistry / FEBS 2001;268(21):5504-20.
2001: Laura Motteran; Mirella S Pilone; Gianluca Molla; Sandro Ghisla; Loredano Pollegioni
Cholesterol oxidase from Brevibacterium sterolicum. The relationship between covalent flavinylation and redox properties.
The Journal of biological chemistry 2001;276(21):18024-30.
2000: Stephan Umhau; Loredano Pollegioni; Gianluca Molla; Kay Diederichs; Wolfram Welte; Mirella S Pilone; Sandro Ghisla
The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(23):12463-8.
2000: Loredano Pollegioni; D Porrini; Gianluca Molla; Mirella S Pilone
Redox potentials and their pH dependence of D-amino-acid oxidase of Rhodotorula gracilis and Trigonopsis variabilis.
European journal of biochemistry / FEBS 2000;267(22):6624-32.
2000: Gianluca Molla; D Porrini; Viviana Job; Laura Motteran; C Vegezzi; S Campaner; Mirella S Pilone; Loredano Pollegioni
Role of arginine 285 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study.
The Journal of biological chemistry 2000;275(32):24715-21.
2000: Davide Vigetti; Claudio Monetti; Loredano Pollegioni; Roberto Taramelli; Giovanni Bernardini
Xenopus allantoicase: molecular cloning, enzymatic activity and developmental expression.
Archives of biochemistry and biophysics 2000;379(1):90-6.
1999: CM Harris; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Studies on the reaction mechanism of Rhodotorula gracilis D-amino-acid oxidase. Role of the highly conserved Tyr-223 on substrate binding and catalysis.
The Journal of biological chemistry 1999;274(51):36233-40.
1999: Loredano Pollegioni; giovanni gadda; D Ambrosius; Sandro Ghisla; Mirella S Pilone
Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum: effect of surfactants and organic solvents on activity.
Biotechnology and applied biochemistry 1999;30 ( Pt 1)():27-33.
1999: Loredano Pollegioni; G Wels; Mirella S Pilone; Sandro Ghisla
Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum.
European journal of biochemistry / FEBS 1999;264(1):140-51.
1998: Gianluca Molla; C Vegezzi; Mirella S Pilone; Loredano Pollegioni
Overexpression in Escherichia coli of a recombinant chimeric Rhodotorula gracilis d-amino acid oxidase.
Protein expression and purification 1998;14(2):289-94.
1998: S Campaner; Loredano Pollegioni; Brian D Ross; Mirella S Pilone
Limited proteolysis and site-directed mutagenesis reveal the origin of microheterogeneity in Rhodotorula gracilis D-amino acid oxidase.
The Biochemical journal 1998;330 ( Pt 2)():615-21.
1998: Lauren D Stegman; H Zheng; E R Neal; Oded Ben-Yoseph; Loredano Pollegioni; Mirella S Pilone; Brian D Ross
Induction of cytotoxic oxidative stress by D-alanine in brain tumor cells expressing Rhodotorula gracilis D-amino acid oxidase: a cancer gene therapy strategy.
Human gene therapy 1998;9(2):185-93.
1997: giovanni gadda; G Wels; Loredano Pollegioni; S Zucchelli; D Ambrosius; Mirella S Pilone; Sandro Ghisla
Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum.
European journal of biochemistry / FEBS 1997;250(2):369-76.
1997: Loredano Pollegioni; Gianluca Molla; S Campaner; E Martegani; Mirella S Pilone
Cloning, sequencing and expression in E. coli of a D-amino acid oxidase cDNA from Rhodotorula gracilis active on cephalosporin C.
Journal of biotechnology 1997;58(2):115-23.
1997: Loredano Pollegioni; S Campaner; A A Raibekas; Mirella S Pilone
Identification of a reactive cysteine in the flavin-binding domain of Rhodotorula gracilis D-amino acid oxidase.
Archives of biochemistry and biophysics 1997;343(1):1-5.
1997: Loredano Pollegioni; W Blodig; Sandro Ghisla
On the mechanism of D-amino acid oxidase. Structure/linear free energy correlations and deuterium kinetic isotope effects using substituted phenylglycines.
The Journal of biological chemistry 1997;272(8):4924-34.
1996: Loredano Pollegioni; Mirella S Pilone
On the holoenzyme reconstitution process in native and truncated Rhodotorula gracilis D-amino acid oxidase.
Archives of biochemistry and biophysics 1996;332(1):58-62.
1996: Loredano Pollegioni; S Campaner; A A Raibekas; Mirella S Pilone
Reactivity of sulfhydryl groups of Rhodotorula gracilis D-amino acid oxidase.
Biochemical Society transactions 1996;24(1):21S.
1995: Loredano Pollegioni; Fabrizio Ceciliani; Bruno Curti; Severino Ronchi; Mirella S Pilone
Studies on the structural and functional aspects of Rhodotorula gracilis D-amino acid oxidase by limited trypsinolysis.
The Biochemical journal 1995;310 ( Pt 2)():577-83.
1994: Loredano Pollegioni; K Fukui; Vincent Massey
Studies on the kinetic mechanism of pig kidney D-amino acid oxidase by site-directed mutagenesis of tyrosine 224 and tyrosine 228.
The Journal of biological chemistry 1994;269(50):31666-73.
1993: Loredano Pollegioni; S Butò; W Tischer; Sandro Ghisla; Mirella S Pilone
Characterization of D-amino acid oxidase from Trigonopsis variabilis.
Biochemistry and molecular biology international 1993;31(4):709-17.
1993: Loredano Pollegioni; B Langkau; W Tischer; Sandro Ghisla; Mirella S Pilone
Kinetic mechanism of D-amino acid oxidases from Rhodotorula gracilis and Trigonopsis variabilis.
The Journal of biological chemistry 1993;268(19):13850-7.
1992: Loredano Pollegioni; Sandro Ghisla; Mirella S Pilone
Studies on the active centre of Rhodotorula gracilis D-amino acid oxidase and comparison with pig kidney enzyme.
The Biochemical journal 1992;286 ( Pt 2)():389-94.
1992: Loredano Pollegioni; Mirella S Pilone
Purification of Rhodotorula gracilis D-amino acid oxidase.
Protein expression and purification 1992;3(2):165-7.
1992: Loredano Pollegioni; A Falbo; Mirella S Pilone
Specificity and kinetics of Rhodotorula gracilis D-amino acid oxidase.
Biochimica et biophysica acta 1992;1120(1):11-6.
1991: Maria-Elisa Perotti; Loredano Pollegioni; Mirella S Pilone
Expression of D-amino acid oxidase in Rhodotorula gracilis under induction conditions: a biochemical and cytochemical study.
European journal of cell biology 1991;55(1):104-13.
1991: P Casalin; Loredano Pollegioni; Bruno Curti; M Pilone Simonetta
A study on apoenzyme from Rhodotorula gracilis D-amino acid oxidase.
European journal of biochemistry / FEBS 1991;197(2):513-7.
1991: Loredano Pollegioni; M P Simonetta
Immunochemical studies on Rhodotorula gracilis D-amino acid oxidase.
Experientia 1991;47(3):232-5.
1989: M Pilone Simonetta; P Casalin; Loredano Pollegioni; Severino Ronchi; Bruno Curti
D-amino-acid oxidase from yeast.
The Italian journal of biochemistry 1989;38(4):296A-297A.
1989: M Pilone Simonetta; Loredano Pollegioni; P Casalin; Bruno Curti; Severino Ronchi
Properties of D-amino-acid oxidase from Rhodotorula gracilis.
European journal of biochemistry / FEBS 1989;180(1):199-204.