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Marina Porcelli

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Giovanna Cacciapuoti; Pompea Del Vecchio; F Fuccio; Luigi Petraccone; Marina Porcelli
Role of disulfide bonds in conformational stability and folding of 5'-deoxy-5'-methylthioadenosine phosphorylase II from the hyperthermophilic archaeon Sulfolobus solfataricus.
Claudia Minici; Marina Porcelli; Giovanna Cacciapuoti; Ester De Leo; Massimo Degano
New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.
Marina Porcelli; Ester De Leo; Pompea Del Vecchio; F Fuccio; Giovanna Cacciapuoti
Thermal unfolding of nucleoside hydrolases from the hyperthermophilic archaeon Sulfolobus solfataricus: role of disulfide bonds.

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All Publications

2012: Giovanna Cacciapuoti; Pompea Del Vecchio; F Fuccio; Luigi Petraccone; Marina Porcelli
Role of disulfide bonds in conformational stability and folding of 5'-deoxy-5'-methylthioadenosine phosphorylase II from the hyperthermophilic archaeon Sulfolobus solfataricus.
Biochimica et biophysica acta 2012;1824(10):1136-43.
2012: Claudia Minici; Marina Porcelli; Giovanna Cacciapuoti; Ester De Leo; Massimo Degano
New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.
Biochemistry 2012;51(22):4590-9.
2012: Marina Porcelli; Ester De Leo; Pompea Del Vecchio; F Fuccio; Giovanna Cacciapuoti
Thermal unfolding of nucleoside hydrolases from the hyperthermophilic archaeon Sulfolobus solfataricus: role of disulfide bonds.
Protein and peptide letters 2012;19(3):369-74.
2011: Giovanna Cacciapuoti; F Fuccio; Anna Marabotti; Marina Porcelli
Unraveling the structural and functional differences between purine nucleoside phosphorylase and 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus.
Biochimica et biophysica acta 2011;1814(10):1358-66.
2009: Giovanna Cacciapuoti; Iolanda Peluso; F Fuccio; Marina Porcelli
Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding.
The FEBS journal 2009;276(20):5799-805.
2009: Caterina Manna; Daniela Napoli; Giovanna Cacciapuoti; Marina Porcelli; Vincenzo Zappia
Olive oil phenolic compounds inhibit homocysteine-induced endothelial cell adhesion regardless of their different antioxidant activity.
Journal of agricultural and food chemistry 2009;57(9):3478-82.
2008: Marina Porcelli; Luigi Concilio; Iolanda Peluso; Anna Marabotti; Angelo Facchiano; Giovanna Cacciapuoti
Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus--biochemical characterization and homology modeling.
The FEBS journal 2008;275(8):1900-14.
2007: Giovanna Cacciapuoti; Caterina Manna; Daniela Napoli; Vincenzo Zappia; Marina Porcelli
Homocysteine-induced endothelial cell adhesion is related to adenosine lowering and is not mediated by S-adenosylhomocysteine.
FEBS letters 2007;581(24):4567-70.
2007: Giovanna Cacciapuoti; Marina Porcelli; Maria Angela Moretti; Francesca Sorrentino; Luigi Concilio; Vincenzo Zappia; Zhi-Jie Liu; Wolfram Tempel; Florian D Schubot; John P Rose; Bi-Cheng Wang; Phillip S Brereton; Francis E Jenney; Michael W W Adams
The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus.
Journal of bacteriology 2007;189(16):6057-67.
2007: Giovanna Cacciapuoti; Sabrina Gorassini; Maria Fiorella Mazzeo; Rosa Anna Siciliano; Virginia Carbone; Vincenzo Zappia; Marina Porcelli
Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus.
The FEBS journal 2007;274(10):2482-95.
2006: Yan Zhang; Marina Porcelli; Giovanna Cacciapuoti; Steven E Ealick
The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds.
Journal of molecular biology 2006;357(1):252-62.
2005: Giovanna Cacciapuoti; Sabrina Forte; Maria Angela Moretti; Assunta Brio; Vincenzo Zappia; Marina Porcelli
A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus.
The FEBS journal 2005;272(8):1886-99.
2005: Marina Porcelli; Maria Angela Moretti; Luigi Concilio; Sabrina Forte; Antonello Merlino; Giuseppe Graziano; Giovanna Cacciapuoti
S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: biochemical characterization and analysis of protein structure by comparative molecular modeling.
Proteins 2005;58(4):815-25.
2004: Giovanna Cacciapuoti; Maria Angela Moretti; Sabrina Forte; Assunta Brio; Laura Camardella; Vincenzo Zappia; Marina Porcelli
Methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus. Mechanism of the reaction and assignment of disulfide bonds.
European journal of biochemistry / FEBS 2004;271(23-24):4834-44.
2003: Giovanna Cacciapuoti; Costanzo Bertoldo; Assunta Brio; Vincenzo Zappia; Marina Porcelli
Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: substrate specificity and primary structure analysis.
Extremophiles : life under extreme conditions 2003;7(2):159-68.
2001: TC Appleby; Irimpan I Mathews; Marina Porcelli; Giovanna Cacciapuoti; Steven E Ealick
Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus.
The Journal of biological chemistry 2001;276(42):39232-42.
2001: Giovanna Cacciapuoti; L Servillo; Maria Angela Moretti; Marina Porcelli
Conformational changes and stabilization induced by phosphate binding to 5'-methylthioadenosine phosphorylase from the thermophilic archaeon Sulfolobus solfataricus.
Extremophiles : life under extreme conditions 2001;5(5):295-302.
2000: Marina Porcelli; S Fusco; T Inizio; Vincenzo Zappia; Giovanna Cacciapuoti
Expression, purification, and characterization of recombinant S-adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfataricus.
Protein expression and purification 2000;18(1):27-35.
1999: Giovanna Cacciapuoti; S Fusco; N Caiazzo; Vincenzo Zappia; Marina Porcelli
Heterologous expression of 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus: characterization of the recombinant protein and involvement of disulfide bonds in thermophilicity and thermostability.
Protein expression and purification 1999;16(1):125-35.
1997: Marina Porcelli; Giovanna Cacciapuoti; S Fusco; Rita Massa; Guglielmo d'Ambrosio; C Bertoldo; M De Rosa; Vincenzo Zappia
Non-thermal effects of microwaves on proteins: thermophilic enzymes as model system.
FEBS letters 1997;402(2-3):102-6.
1996: Marina Porcelli; Giovanna Cacciapuoti; S Fusco; C Bertoldo; M De Rosa; Vincenzo Zappia
Cloning and sequencing of the gene coding for S-adenosylhomocysteine hydrolase in the thermophilic archaeon Sulfolobus solfataricus.
Gene 1996;177(1-2):17-22.
1996: Giovanna Cacciapuoti; Marina Porcelli; C Bertoldo; S Fusco; M De Rosa; Vincenzo Zappia
Extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Gene cloning and amino acid sequence determination.
European journal of biochemistry / FEBS 1996;239(3):632-7.
1994: Giovanna Cacciapuoti; Marina Porcelli; C Bertoldo; M De Rosa; Vincenzo Zappia
Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds.
The Journal of biological chemistry 1994;269(40):24762-9.
1993: Marina Porcelli; Giovanna Cacciapuoti; S Fusco; Giuseppe Iacomino; Agata Gambacorta; M De Rosa; Vincenzo Zappia
S-adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfataricus: purification, physico-chemical and immunological properties.
Biochimica et biophysica acta 1993;1164(2):179-88.
1992: Raffaele Ragone; francesco facchiano; Giovanna Cacciapuoti; Marina Porcelli; Giovanni Colonna
Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 2. Denaturation and structural stability.
European journal of biochemistry / FEBS 1992;204(2):483-90.
1992: francesco facchiano; Raffaele Ragone; Marina Porcelli; Giovanna Cacciapuoti; Giovanni Colonna
Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 1. Conformational behavior of the oligomeric enzyme in solution.
European journal of biochemistry / FEBS 1992;204(2):473-82.
1991: Giovanna Cacciapuoti; Marina Porcelli; M De Rosa; Agata Gambacorta; C Bertoldo; Vincenzo Zappia
S-adenosylmethionine decarboxylase from the thermophilic archaebacterium Sulfolobus solfataricus. Purification, molecular properties and studies on the covalently bound pyruvate.
European journal of biochemistry / FEBS 1991;199(2):395-400.
1990: Raffaele Ragone; francesco facchiano; Giovanna Cacciapuoti; Marina Porcelli; Giovanni Colonna
Thermodynamic analysis of the temperature effect on the conformational behaviour of propylaminetransferase from Sulfolobus solfataricus.
The Italian journal of biochemistry 1990;39(3):186A-188A.
1989: Marina Porcelli; Giovanna Cacciapuoti; Guido Cimino; M Gavagnin; G Sodano; Vincenzo Zappia
Biosynthesis and metabolism of 9-[5'-deoxy-5'-(methylthio)-beta-D-xylofuranosyl]adenine, a novel natural analogue of methylthioadenosine.
The Biochemical journal 1989;263(3):635-40.
1988: Marina Porcelli; Giovanna Cacciapuoti; M Cartení-Farina; Agata Gambacorta
S-adenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus. Purification and characterization of two isoforms.
European journal of biochemistry / FEBS 1988;177(2):273-80.
1988: Marina Porcelli; Giovanna Cacciapuoti; Adriana Oliva; Vincenzo Zappia
High-performance liquid chromatographic determination of a naturally occurring xylosyl thioether, 9-[5'-deoxy-5'(methylthio)-beta-D-xylofuranosyl]adenine.
Journal of chromatography 1988;440():151-5.
1988: Marina Porcelli; Giovanna Cacciapuoti; Guido Cimino; M Gavagnin; G Sodano; Vincenzo Zappia
Characterization and biogenesis of 5'-methylthioxylofuranosyl adenine, a new natural analog of 5'-methylthioadenosine.
Advances in experimental medicine and biology 1988;250():219-28.
1988: Giovanna Cacciapuoti; Marina Porcelli; Agata Gambacorta; T Romano; M De Rosa
S-adenosylmethionine decarboxylase from the thermophilic archaebacterium Sulfolobus solfataricus.
Advances in experimental medicine and biology 1988;250():91-100.
1986: Giovanna Cacciapuoti; Marina Porcelli; M Cartenì-Farina; Agata Gambacorta; Vincenzo Zappia
Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium.
European journal of biochemistry / FEBS 1986;161(2):263-71.
1985: Fulvio Della Ragione; Marina Porcelli; M Cartenì-Farina; Vincenzo Zappia; A E Pegg
Escherichia coli S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase. Purification, substrate specificity and mechanism of action.
The Biochemical journal 1985;232(2):335-41.
1984: M Cartenì-Farina; Giovanna Cacciapuoti; Marina Porcelli; Fulvio Della Ragione; M Lancieri; Giuseppe Geraci; Vincenzo Zappia
Studies on the metabolic effects of methylthioformycin.
Biochimica et biophysica acta 1984;805(2):158-64.
1984: P P Di Fiore; M Grieco; A Pinto; V Attadia; Marina Porcelli; Giovanna Cacciapuoti; M Cartenì-Farina
Inhibition of dimethyl sulfoxide-induced differentiation of Friend erythroleukemic cells by 5'-methylthioadenosine.
Cancer research 1984;44(9):4096-103.
1983: M Cartenì-Farina; Fulvio Della Ragione; Giovanna Cacciapuoti; Marina Porcelli; Vincenzo Zappia
Transport and metabolism of 5'-methylthioadenosine in human erythrocytes.
Biochimica et biophysica acta 1983;727(2):221-9.
1981: Vincenzo Zappia; Fulvio Della Ragione; Marina Porcelli; Giovanna Cacciapuoti; M Carteni-Farina
Studies on 5'-methylthioadenosine uptake by human erythrocytes.
Medical biology 1981;59(5-6):354-8.
1981: Fulvio Della Ragione; M Cartenì-Farina; Marina Porcelli; Giovanna Cacciapuoti; Vincenzo Zappia
High-performance liquid chromatographic analysis of 5'-methylthioadenosine in rat tissues.
Journal of chromatography 1981;226(1):243-9.
1981: Giovanna Cacciapuoti; Fulvio Della Ragione; Marina Porcelli; M Cartenì-Farina
[New method of synthesis of structural analogs of 5'-methylthioadenosine].
Bollettino della Società italiana di biologia sperimentale 1981;57(10):1082-8.
1980: Fulvio Della Ragione; G Ragosta; Giovanna Cacciapuoti; Marina Porcelli; M Cartenì-Farina
[A new method of measuring 5'-methylthioadenosine with high pressure liquid chromatography].
Bollettino della Società italiana di biologia sperimentale 1980;56(19):2030-6.
1980: Vincenzo Zappia; Patrizia Galletti; Marina Porcelli; Caterina Manna; Fulvio Della Ragione
High-performance liquid chromatographic separation of natural adenosyl-sulphur compounds.
Journal of chromatography 1980;189(3):399-405.
1980: Fulvio Della Ragione; Marina Porcelli; Caterina Manna; G Romeo; Patrizia Galletti
[A new method for determination of adenosylmethionine, adenosyl-homocysteine and decarboxylated adenosylmethionine].
Bollettino della Società italiana di biologia sperimentale 1980;56(3):250-6.
1980: Marina Porcelli; Fulvio Della Ragione; Giovanna Cacciapuoti; M Cartenì-Farina
[S-adenosyl(5')-3-methylthiopropylamine as substrate and inhibitor of S-adenosylmethionine lyase from rat liver].
Bollettino della Società italiana di biologia sperimentale 1980;56(3):245-9.
1980: Giovanna Cacciapuoti; Marina Porcelli; Fulvio Della Ragione; G Pontoni; M Cartenì-Farina
[New 5'-sulfurated nucleosides as inhibitors of the biosynthesis of polyamines].
Bollettino della Società italiana di biologia sperimentale 1980;56(3):238-44.