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Anthony Pugsley

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Publications

TOP 3
Nicholas N Nickerson; Sophie S Abby; Mohamed Chami; Eduardo P C Rocha; Anthony P Pugsley
A Single Amino Acid Substitution Changes the Self-Assembly Status of a Type IV Piliation Secretin.
David A Cisneros; Peter J Bond; Manuel Campos; Anthony P Pugsley; Olivera Francetic
Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation.
Anthony P Pugsley
Why study Escherichia coli?

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All Publications

2012: Nicholas N Nickerson; Sophie S Abby; Mohamed Chami; Eduardo P C Rocha; Anthony P Pugsley
A Single Amino Acid Substitution Changes the Self-Assembly Status of a Type IV Piliation Secretin.
Journal of bacteriology 2012;194(18):4951-8.
2012: David A Cisneros; Peter J Bond; Manuel Campos; Anthony P Pugsley; Olivera Francetic
Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation.
The EMBO journal 2012;31(4):1041-53.
2012: Anthony P Pugsley
Why study Escherichia coli?
Research in microbiology 2012;163(2):81-2.
2011: Nicholas N Nickerson; Bruno Baron; Andréa Dessen; Patrick England; Bertrand Raynal; Tommaso Tosi; Anthony P Pugsley
Outer membrane targeting of secretin PulD protein relies on disordered domain recognition by a dedicated chaperone.
The Journal of biological chemistry 2011;286(45):38833-43.
2011: Séverine Collin; Ingrid Guilvout; Nicholas N Nickerson; Anthony P Pugsley
Sorting of an integral outer membrane protein via the lipoprotein-specific Lol pathway and a dedicated lipoprotein pilotin.
Molecular microbiology 2011;80(3):655-65.
2011: Ingrid Guilvout; Nicholas N Nickerson; MOUNIA CHAMI; Anthony P Pugsley
Multimerization-defective variants of dodecameric secretin PulD.
Research in microbiology 2011;162(2):180-90.
2011: Catherine Berrier; Ingrid Guilvout; Nicolas Bayan; Kyu-Ho Park; Agnes Mesneau; MOUNIA CHAMI; Anthony P Pugsley; Alexandre Ghazi
Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL MscL does not need the insertase YidC for insertion in vitro.
Biochimica et biophysica acta 2011;1808(1):41-6.
2008: Martin Krehenbrink; Mohamed Chami; Ingrid Guilvout; Pedro Alzari; Frédéric Pécorari; Anthony P Pugsley
Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD.
Journal of molecular biology 2008;383(5):1058-68.
2008: Ingrid Guilvout; Mohamed Chami; Catherine Berrier; Alexandre Ghazi; Andreas Engel; Anthony P Pugsley; Nicolas Bayan
In vitro multimerization and membrane insertion of bacterial outer membrane secretin PulD.
Journal of molecular biology 2008;382(1):13-23.
2008: Shawn Lewenza; Musa M Mhlanga; Anthony P Pugsley
Novel inner membrane retention signals in Pseudomonas aeruginosa lipoproteins.
Journal of bacteriology 2008;190(18):6119-25.
2007: Barbara Mouratou; Francis Schaeffer; Ingrid Guilvout; Diana Tello-Manigne; Anthony P Pugsley; Pedro Alzari; Frédéric Pecorari
Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(46):17983-8.
2007: Séverine Collin; Ingrid Guilvout; Mohamed Chami; Anthony P Pugsley
YaeT-independent multimerization and outer membrane association of secretin PulD.
Molecular microbiology 2007;64(5):1350-7.
2007: Olivera Francetic; Nienke Buddelmeijer; Shawn Lewenza; Carol A Kumamoto; Anthony P Pugsley
Signal recognition particle-dependent inner membrane targeting of the PulG Pseudopilin component of a type II secretion system.
Journal of bacteriology 2007;189(5):1783-93.
2006: Ingrid Guilvout; Mohamed Chami; Andreas Engel; Anthony P Pugsley; Nicolas Bayan
Bacterial outer membrane secretin PulD assembles and inserts into the inner membrane in the absence of its pilotin.
The EMBO journal 2006;25(22):5241-9.
2006: Anastassios Economou; Peter J Christie; Rachel C Fernandez; Tracy Palmer; Gregory V Plano; Anthony P Pugsley
Secretion by numbers: Protein traffic in prokaryotes.
Molecular microbiology 2006;62(2):308-19.
2006: Shawn Lewenza; Dominique Vidal-Ingigliardi; Anthony P Pugsley
Direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family Enterobacteriaceae.
Journal of bacteriology 2006;188(10):3516-24.
2006: Nienke Buddelmeijer; Olivera Francetic; Anthony P Pugsley
Green fluorescent chimeras indicate nonpolar localization of pullulanase secreton components PulL and PulM.
Journal of bacteriology 2006;188(8):2928-35.
2006: Nicolas Bayan; Ingrid Guilvout; Anthony P Pugsley
Secretins take shape.
Molecular microbiology 2006;60(1):1-4.
2005: Mohamed Chami; Ingrid Guilvout; Marco Gregorini; Hervé-W Rémigy; Shirley A Müller; Marielle Valerio; Andreas Engel; Anthony P Pugsley; Nicolas Bayan
Structural insights into the secretin PulD and its trypsin-resistant core.
The Journal of biological chemistry 2005;280(45):37732-41.
2005: Olivera Francetić; Anthony P Pugsley
Towards the identification of type II secretion signals in a nonacylated variant of pullulanase from Klebsiella oxytoca.
Journal of bacteriology 2005;187(20):7045-55.
2005: Carine Robichon; Dominique Vidal-Ingigliardi; Anthony P Pugsley
Depletion of apolipoprotein N-acyltransferase causes mislocalization of outer membrane lipoproteins in Escherichia coli.
The Journal of biological chemistry 2005;280(2):974-83.
2004: Rolf Köhler; Karsten Schäfer; Shirley Müller; Guillaume Vignon; Kay Diederichs; Ansgar Philippsen; Philippe Ringler; Anthony P Pugsley; Andreas Engel; Wolfram Welte
Structure and assembly of the pseudopilin PulG.
Molecular microbiology 2004;54(3):647-64.
2004: Anthony P Pugsley; Nienke Buddelmeijer
Traffic spotting: poles apart.
Molecular microbiology 2004;53(6):1559-62.
2004: Anthony P Pugsley; Olivera Francetic; Arnold J Driessen; Victor de Lorenzo
Getting out: protein traffic in prokaryotes.
Molecular microbiology 2004;52(1):3-11.
2003: Christopher R Peabody; Yong Joon Chung; Ming-Ren Yen; Dominique Vidal-Ingigliardi; Anthony P Pugsley; Milton H Saier
Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella.
Microbiology (Reading, England) 2003;149(Pt 11):3051-72.
2003: Carine Robichon; Melanie BONHIVERS; Anthony P Pugsley
An intramolecular disulphide bond reduces the efficacy of a lipoprotein plasma membrane sorting signal.
Molecular microbiology 2003;49(4):1145-54.
2003: Charalambos Pozidis; Aggeliki Chalkiadaki; Amalia Gomez-Serrano; Henning Stahlberg; Ian Brown; Anastasia P Tampakaki; Ariel Lustig; Giorgos Sianidis; Anastasia S Politou; Andreas Engel; Nickolas J Panopoulos; John Mansfield; Anthony P Pugsley; Spyridoula Karamanou; Anastassios Economou
Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization.
The Journal of biological chemistry 2003;278(28):25816-24.
2003: Guillaume Vignon; Rolf Köhler; Eric Larquet; Stéphanie Giroux; Marie-Christine Prévost; Pascal Roux; Anthony P Pugsley
Type IV-like pili formed by the type II secreton: specificity, composition, bundling, polar localization, and surface presentation of peptides.
Journal of bacteriology 2003;185(11):3416-28.
2001: Anthony P Pugsley; Nicolas Bayan; Nathalie Sauvonnet
Disulfide bond formation in secreton component PulK provides a possible explanation for the role of DsbA in pullulanase secretion.
Journal of bacteriology 2001;183(4):1312-9.
2000: Olivera Francetic; D Belin; cyril badaut; Anthony P Pugsley
Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion.
The EMBO journal 2000;19(24):6697-703.
2000: N Nouwen; Henning Stahlberg; Anthony P Pugsley; Andreas Engel
Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy.
The EMBO journal 2000;19(10):2229-36.
2000: Nathalie Sauvonnet; Guillaume Vignon; Anthony P Pugsley; Pierre GOUNON
Pilus formation and protein secretion by the same machinery in Escherichia coli.
The EMBO journal 2000;19(10):2221-8.
2000: OM Possot; Guillaume Vignon; N Bomchil; Frank Ebel; Anthony P Pugsley
Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE.
Journal of bacteriology 2000;182(8):2142-52.
2000: Olivera Francetic; cyril badaut; Sylvie Rimsky; Anthony P Pugsley
The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS.
Molecular microbiology 2000;35(6):1506-17.
2000: Nathalie Sauvonnet; Pierre GOUNON; Anthony P Pugsley
PpdD type IV pilin of Escherichia coli K-12 can Be assembled into pili in Pseudomonas aeruginosa.
Journal of bacteriology 2000;182(3):848-54.
1999: Ingrid Guilvout; Kim Hardie; Nathalie Sauvonnet; Anthony P Pugsley
Genetic dissection of the outer membrane secretin PulD: are there distinct domains for multimerization and secretion specificity?
Journal of bacteriology 1999;181(23):7212-20.
1999: A Seydel; Pierre GOUNON; Anthony P Pugsley
Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection.
Molecular microbiology 1999;34(4):810-21.
1999: N Nouwen; N Ranson; H Saibil; B Wolpensinger; Andreas Engel; A Ghazi; Anthony P Pugsley
Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(14):8173-7.
1999: OM Possot; M Gérard-Vincent; Anthony P Pugsley
Membrane association and multimerization of secreton component pulC.
Journal of bacteriology 1999;181(13):4004-11.
1998: Anthony P Pugsley; Olivera Francetic
Protein secretion in Escherichia coli K-12: dead or alive?
Cellular and molecular life sciences : CMLS 1998;54(4):347-52.
1998: O Francetić; S Lory; Anthony P Pugsley
A second prepilin peptidase gene in Escherichia coli K-12.
Molecular microbiology 1998;27(4):763-75.
1998: Nathalie Sauvonnet; Anthony P Pugsley
The requirement for DsbA in pullulanase secretion is independent of disulphide bond formation in the enzyme.
Molecular microbiology 1998;27(3):661-7.
1997: P Mourrain; Iñigo Lasa; A Gautreau; Edith Gouin; Anthony P Pugsley; Pascale Cossart
ActA is a dimer.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(19):10034-9.
1997: OM Possot; Anthony P Pugsley
The conserved tetracysteine motif in the general secretory pathway component PulE is required for efficient pullulanase secretion.
Gene 1997;192(1):45-50.
1997: Anthony P Pugsley; Olivera Francetic; OM Possot; Nathalie Sauvonnet; Kim Hardie
Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria--a review.
Gene 1997;192(1):13-9.
1997: S Daefler; Ingrid Guilvout; Kim Hardie; Anthony P Pugsley; Marjorie Russel
The C-terminal domain of the secretin PulD contains the binding site for its cognate chaperone, PulS, and confers PulS dependence on pIVf1 function.
Molecular microbiology 1997;24(3):465-75.
1997: OM Possot; L Letellier; Anthony P Pugsley
Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway.
Molecular microbiology 1997;24(3):457-64.
1997: Anthony P Pugsley; Olivera Francetic; Kim Hardie; OM Possot; Nathalie Sauvonnet; A Seydel
Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria.
Folia microbiologica 1997;42(3):184-92.
1996: Kim Hardie; A Seydel; Ingrid Guilvout; Anthony P Pugsley
The secretin-specific, chaperone-like protein of the general secretory pathway: separation of proteolytic protection and piloting functions.
Molecular microbiology 1996;22(5):967-76.
1996: Nathalie Sauvonnet; Anthony P Pugsley
Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting beta-lactamase secretion by the general secretory pathway.
Molecular microbiology 1996;22(1):1-7.
1996: Anthony P Pugsley
Bacterial toxins deliver the goods.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(16):8155-6.
1996: Anthony P Pugsley
Multimers of the precursor of a type IV pilin-like component of the general secretory pathway are unrelated to pili.
Molecular microbiology 1996;20(6):1235-45.
1996: Olivera Francetic; Anthony P Pugsley
The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins.
Journal of bacteriology 1996;178(12):3544-9.
1996: Kim Hardie; S Lory; Anthony P Pugsley
Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein.
The EMBO journal 1996;15(5):978-88.
1995: Nathalie Sauvonnet; I Poquet; Anthony P Pugsley
Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N- or C-terminal end.
Journal of bacteriology 1995;177(18):5238-46.
1995: Ingrid Guilvout; Elisabeth Carniel; Anthony P Pugsley
Yersinia spp. HMWP2, a cytosolic protein with a cryptic internal signal sequence which can promote alkaline phosphatase export.
Journal of bacteriology 1995;177(7):1780-7.
1994: OM Possot; Anthony P Pugsley
Molecular characterization of PulE, a protein required for pullulanase secretion.
Molecular microbiology 1994;12(2):287-99.
1994: B Dupuy; Anthony P Pugsley
Type IV prepilin peptidase gene of Neisseria gonorrhoeae MS11: presence of a related gene in other piliated and nonpiliated Neisseria strains.
Journal of bacteriology 1994;176(5):1323-31.
1993: Anthony P Pugsley; OM Possot
The general secretory pathway of Klebsiella oxytoca: no evidence for relocalization or assembly of pilin-like PulG protein into a multiprotein complex.
Molecular microbiology 1993;10(3):665-74.
1993: Ingrid Guilvout; Odile Mercereau-Puijalon; Serge Bonnefoy; Anthony P Pugsley; Elisabeth Carniel
High-molecular-weight protein 2 of Yersinia enterocolitica is homologous to AngR of Vibrio anguillarum and belongs to a family of proteins involved in nonribosomal peptide synthesis.
Journal of bacteriology 1993;175(17):5488-504.
1993: I Poquet; M G Kornacker; Anthony P Pugsley
The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion.
Molecular microbiology 1993;9(5):1061-9.
1993: Anthony P Pugsley
Processing and methylation of PuIG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca.
Molecular microbiology 1993;9(2):295-308.
1993: Anthony P Pugsley
A mutation in the dsbA gene coding for periplasmic disulfide oxidoreductase reduces transcription of the Escherichia coli ompF gene.
Molecular & general genetics : MGG 1993;237(3):407-11.
1993: Anthony P Pugsley
The complete general secretory pathway in gram-negative bacteria.
Microbiological reviews 1993;57(1):50-108.
1993: I Poquet; D Faucher; Anthony P Pugsley
Stable periplasmic secretion intermediate in the general secretory pathway of Escherichia coli.
The EMBO journal 1993;12(1):271-8.
1992: Anthony P Pugsley
Translocation of a folded protein across the outer membrane in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(24):12058-62.
1992: B Dupuy; Muhamed-Kheir Taha; OM Possot; C Marchal; Anthony P Pugsley
PulO, a component of the pullulanase secretion pathway of Klebsiella oxytoca, correctly and efficiently processes gonococcal type IV prepilin in Escherichia coli.
Molecular microbiology 1992;6(14):1887-94.
1992: Anthony P Pugsley; B Dupuy
An enzyme with type IV prepilin peptidase activity is required to process components of the general extracellular protein secretion pathway of Klebsiella oxytoca.
Molecular microbiology 1992;6(6):751-60.
1992: OM Possot; Christophe d'Enfert; I Reyss; Anthony P Pugsley
Pullulanase secretion in Escherichia coli K-12 requires a cytoplasmic protein and a putative polytopic cytoplasmic membrane protein.
Molecular microbiology 1992;6(1):95-105.
1991: B Dupuy; Muhamed-Kheir Taha; Anthony P Pugsley; C Marchal
Neisseria gonorrhoeae prepilin export studied in Escherichia coli.
Journal of bacteriology 1991;173(23):7589-98.
1991: M G Kornacker; D Faucher; Anthony P Pugsley
Outer membrane translocation of the extracellular enzyme pullulanase in Escherichia coli K12 does not require a fatty acylated N-terminal cysteine.
The Journal of biological chemistry 1991;266(21):13842-8.
1991: Anthony P Pugsley; M G Kornacker
Secretion of the cell surface lipoprotein pullulanase in Escherichia coli. Cooperation or competition between the specific secretion pathway and the lipoprotein sorting pathway.
The Journal of biological chemistry 1991;266(21):13640-5.
1991: Anthony P Pugsley; I Poquet; M G Kornacker
Two distinct steps in pullulanase secretion by Escherichia coli K12.
Molecular microbiology 1991;5(4):865-73.
1991: Anthony P Pugsley; M G Kornacker; I Poquet
The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12.
Molecular microbiology 1991;5(2):343-52.
1990: M G Kornacker; Anthony P Pugsley
The normally periplasmic enzyme beta-lactamase is specifically and efficiently translocated through the Escherichia coli outer membrane when it is fused to the cell-surface enzyme pullulanase.
Molecular microbiology 1990;4(7):1101-9.
1990: I Reyss; Anthony P Pugsley
Five additional genes in the pulC-O operon of the gram-negative bacterium Klebsiella oxytoca UNF5023 which are required for pullulanase secretion.
Molecular & general genetics : MGG 1990;222(2-3):176-84.
1990: Anthony P Pugsley; I Reyss
Five genes at the 3' end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion.
Molecular microbiology 1990;4(3):365-79.
1990: M G Kornacker; Anthony P Pugsley
Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023.
Molecular microbiology 1990;4(1):73-85.
1990: Anthony P Pugsley; M G Kornacker; A Ryter
Analysis of the subcellular location of pullulanase produced by Escherichia coli carrying the pulA gene from Klebsiella pneumoniae strain UNF5023.
Molecular microbiology 1990;4(1):59-72.
1990: Anthony P Pugsley; Christophe d'Enfert; I Reyss; M G Kornacker
Genetics of extracellular protein secretion by gram-negative bacteria.
Annual review of genetics 1990;24():67-90.
1989: Christophe d'Enfert; I Reyss; Cécile Wandersman; Anthony P Pugsley
Protein secretion by gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K-12.
The Journal of biological chemistry 1989;264(29):17462-8.
1989: Christophe d'Enfert; Anthony P Pugsley
Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion.
Journal of bacteriology 1989;171(7):3673-9.
1988: Anthony P Pugsley; C Dubreuil
Molecular characterization of malQ, the structural gene for the Escherichia coli enzyme amylomaltase.
Molecular microbiology 1988;2(4):473-9.
1988: Anthony P Pugsley
The immunity and lysis genes of ColN plasmid pCHAP4.
Molecular & general genetics : MGG 1988;211(2):335-41.
1987: Anthony P Pugsley; W Zimmerman; W Wehrli
Highly efficient uptake of a rifamycin derivative via the FhuA-TonB-dependent uptake route in Escherichia coli.
Journal of general microbiology 1987;133(12):3505-11.
1987: Christophe d'Enfert; A Ryter; Anthony P Pugsley
Cloning and expression in Escherichia coli of the Klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase.
The EMBO journal 1987;6(11):3531-8.
1987: Anthony P Pugsley
Nucleotide sequencing of the structural gene for colicin N reveals homology between the catalytic, C-terminal domains of colicins A and N.
Molecular microbiology 1987;1(3):317-25.
1987: Christophe d'Enfert; Anthony P Pugsley
A gene fusion approach to the study of pullulanase export and secretion in Escherichia coli.
Molecular microbiology 1987;1(2):159-68.
1987: Anthony P Pugsley; S T Cole
An unmodified form of the ColE2 lysis protein, an envelope lipoprotein, retains reduced ability to promote colicin E2 release and lysis of producing cells.
Journal of general microbiology 1987;133(9):2411-20.
1987: Christophe d'Enfert; C Chapon; Anthony P Pugsley
Export and secretion of the lipoprotein pullulanase by Klebsiella pneumoniae.
Molecular microbiology 1987;1(1):107-16.
1986: Anthony P Pugsley; F Moreno; V de Lorenzo
Microcin-E492-insensitive mutants of Escherichia coli K12.
Journal of general microbiology 1986;132(12):3253-9.
1985: S Michaelis; C Chapon; Christophe d'Enfert; Anthony P Pugsley; M Schwartz
Characterization and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae.
Journal of bacteriology 1985;164(2):633-8.
1984: Anthony P Pugsley
The ins and outs of colicins. Part II. Lethal action, immunity and ecological implications.
Microbiological sciences 1984;1(8):203-5.
1984: Anthony P Pugsley
The ins and outs of colicins. Part I: Production, and translocation across membranes.
Microbiological sciences 1984;1(7):168-75.