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Alfred Pühler

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Co-Publications
Name
76
Kalinowski, Jörn
52
Tauch, Andreas
35
Becker, Anke
32
Goesmann, Alexander
24
Küster, Helge
24
Schlüter, Andreas
22
Meyer, Folker
19
Niehaus, Karsten
18
Selbitschka, Werner
18
Szczepanowski, Rafael
18
Bekel, Thomas
17
Schneiker, Susanne
17
Perlick, Andreas
14
Rückert, Christian
14
Linke, Burkhard

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Publications

TOP 3
Szczepanowski Rafael; Linke Burkhard; Krahn Irene; Gartemann Karl-Heinz; Gützkow Tim; Eichler Wolfgang; Pühler Alfred; Schlüter Andreas
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
Kröber Magdalena; Bekel Thomas; Diaz Naryttza N; Goesmann Alexander; Jaenicke Sebastian; Krause Lutz; Miller Dimitri; Runte Kai J; Viehöver Prisca; Pühler Alfred; Schlüter Andreas
Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing.
Jochmann Nina; Kurze Anna-Katharina; Czaja Lisa F; Brinkrolf Karina; Brune Iris; Hüser Andrea T; Hansmeier Nicole; Pühler Alfred; Borovok Ilya; Tauch Andreas
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.

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All Publications

2009: Szczepanowski Rafael; Linke Burkhard; Krahn Irene; Gartemann Karl-Heinz; Gützkow Tim; Eichler Wolfgang; Pühler Alfred; Schlüter Andreas
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
Microbiology (Reading, England) 2009;155(Pt 7):2306-19.
2009: Kröber Magdalena; Bekel Thomas; Diaz Naryttza N; Goesmann Alexander; Jaenicke Sebastian; Krause Lutz; Miller Dimitri; Runte Kai J; Viehöver Prisca; Pühler Alfred; Schlüter Andreas
Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing.
Journal of biotechnology 2009;142(1):38-49.
2009: Jochmann Nina; Kurze Anna-Katharina; Czaja Lisa F; Brinkrolf Karina; Brune Iris; Hüser Andrea T; Hansmeier Nicole; Pühler Alfred; Borovok Ilya; Tauch Andreas
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.
Microbiology (Reading, England) 2009;155(Pt 5):1459-77.
2009: Strittmatter Axel W; Liesegang Heiko; Rabus Ralf; Decker Iwona; Amann Judith; Andres Sönke; Henne Anke; Fricke Wolfgang Florian; Martinez-Arias Rosa; Bartels Daniela; Goesmann Alexander; Krause Lutz; Pühler Alfred; Klenk Hans-Peter; Richter Michael; Schüler Margarete; Glöckner Frank Oliver; Meyerdierks Anke; Gottschalk Gerhard; Amann Rudolf
Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide.
Environmental microbiology 2009;11(5):1038-55.
2009: Bekel Thomas; Henckel Kolja; Küster Helge; Meyer Folker; Mittard Runte Virginie; Neuweger Heiko; Paarmann Daniel; Rupp Oliver; Zakrzewski Martha; Pühler Alfred; Stoye Jens; Goesmann Alexander
The Sequence Analysis and Management System -- SAMS-2.0: data management and sequence analysis adapted to changing requirements from traditional sanger sequencing to ultrafast sequencing technologies.
Journal of biotechnology 2009;140(1-2):3-12.
2009: Pühler Alfred; Selbitschka Werner
Functional genome research on bacteria relevant for agriculture, environment and biotechnology.
Journal of biotechnology 2009;140(1-2):1-2.
2009: Becker Anke; Barnett Melanie J; Capela Delphine; Dondrup Michael; Kamp Paul-Bertram; Krol Elizaveta; Linke Burkhard; Rüberg Silvia; Runte Kai; Schroeder Brenda K; Weidner Stefan; Yurgel Svetlana N; Batut Jacques; Long Sharon R; Pühler Alfred; Goesmann Alexander
A portal for rhizobial genomes: RhizoGATE integrates a Sinorhizobium meliloti genome annotation update with postgenome data.
Journal of biotechnology 2009;140(1-2):45-50.
2009: Nentwich Svenja S; Brinkrolf Karina; Gaigalat Lars; Hüser Andrea T; Rey Daniel A; Mohrbach Tobias; Marin Kay; Pühler Alfred; Tauch Andreas; Kalinowski Jörn
Characterization of the LacI-type transcriptional repressor RbsR controlling ribose transport in Corynebacterium glutamicum ATCC 13032.
Microbiology (Reading, England) 2009;155(Pt 1):150-64.
2009: Blom Jochen; Albaum Stefan P; Doppmeier Daniel; Pühler Alfred; Vorhölter Frank-Jörg; Zakrzewski Martha; Goesmann Alexander
EDGAR: a software framework for the comparative analysis of prokaryotic genomes.
BMC bioinformatics 2009;10():154.
2009: Hellweg Christoph; Pühler Alfred; Weidner Stefan
The time course of the transcriptomic response of Sinorhizobium meliloti 1021 following a shift to acidic pH.
BMC microbiology 2009;9():37.
2009: Dondrup Michael; Albaum Stefan P; Griebel Thasso; Henckel Kolja; Jünemann Sebastian; Kahlke Tim; Kleindt Christiane K; Küster Helge; Linke Burkhard; Mertens Dominik; Mittard-Runte Virginie; Neuweger Heiko; Runte Kai J; Tauch Andreas; Tille Felix; Pühler Alfred; Goesmann Alexander
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
BMC bioinformatics 2009;10():50.
2008: Strauch Eckhard; Hammerl Jens Andre; Konietzny Antje; Schneiker-Bekel Susanne; Arnold Walter; Goesmann Alexander; Pühler Alfred; Beutin Lothar
Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7.
Infection and immunity 2008;76(12):5466-77.
2008: Pühler Alfred; Selbitschka Werner
Genome research in the light of ultrafast sequencing technologies.
Journal of biotechnology 2008;136(1-2):1-2.
2008: Krause Lutz; Diaz Naryttza N; Edwards Robert A; Gartemann Karl-Heinz; Krömeke Holger; Neuweger Heiko; Pühler Alfred; Runte Kai J; Schlüter Andreas; Stoye Jens; Szczepanowski Rafael; Tauch Andreas; Goesmann Alexander
Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor.
Journal of biotechnology 2008;136(1-2):91-101.
2008: Schlüter Andreas; Krause Lutz; Szczepanowski Rafael; Goesmann Alexander; Pühler Alfred
Genetic diversity and composition of a plasmid metagenome from a wastewater treatment plant.
Journal of biotechnology 2008;136(1-2):65-76.
2008: Schlüter Andreas; Bekel Thomas; Diaz Naryttza N; Dondrup Michael; Eichenlaub Rudolf; Gartemann Karl-Heinz; Krahn Irene; Krause Lutz; Krömeke Holger; Kruse Olaf; Mussgnug Jan H; Neuweger Heiko; Niehaus Karsten; Pühler Alfred; Runte Kai J; Szczepanowski Rafael; Tauch Andreas; Tilker Alexandra; Viehöver Prisca; Goesmann Alexander
The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology.
Journal of biotechnology 2008;136(1-2):77-90.
2008: Szczepanowski Rafael; Bekel Thomas; Goesmann Alexander; Krause Lutz; Krömeke Holger; Kaiser Olaf; Eichler Wolfgang; Pühler Alfred; Schlüter Andreas
Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology.
Journal of biotechnology 2008;136(1-2):54-64.
2008: Stiens Michael; Becker Anke; Bekel Thomas; Gödde Victoria; Goesmann Alexander; Niehaus Karsten; Schneiker-Bekel Susanne; Selbitschka Werner; Weidner Stefan; Schlüter Andreas; Pühler Alfred
Comparative genomic hybridisation and ultrafast pyrosequencing revealed remarkable differences between the Sinorhizobium meliloti genomes of the model strain Rm1021 and the field isolate SM11.
Journal of biotechnology 2008;136(1-2):31-7.
2008: Tauch Andreas; Schneider Jessica; Szczepanowski Rafael; Tilker Alexandra; Viehoever Prisca; Gartemann Karl-Heinz; Arnold Walter; Blom Jochen; Brinkrolf Karina; Brune Iris; Götker Susanne; Weisshaar Bernd; Goesmann Alexander; Dröge Marcus; Pühler Alfred
Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids.
Journal of biotechnology 2008;136(1-2):22-30.
2008: Tauch Andreas; Trost Eva; Tilker Alexandra; Ludewig Ulrike; Schneiker Susanne; Goesmann Alexander; Arnold Walter; Bekel Thomas; Brinkrolf Karina; Brune Iris; Götker Susanne; Kalinowski Jörn; Kamp Paul-Bertram; Lobo Francisco Pereira; Viehoever Prisca; Weisshaar Bernd; Soriano Francisco; Dröge Marcus; Pühler Alfred
The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing.
Journal of biotechnology 2008;136(1-2):11-21.
2008: Kohl Thomas A; Baumbach Jan; Jungwirth Britta; Pühler Alfred; Tauch Andreas
The GlxR regulon of the amino acid producer Corynebacterium glutamicum: in silico and in vitro detection of DNA binding sites of a global transcription regulator.
Journal of biotechnology 2008;135(4):340-50.
2008: Serrania Javier; Vorhölter Frank-Jörg; Niehaus Karsten; Pühler Alfred; Becker Anke
Identification of Xanthomonas campestris pv. campestris galactose utilization genes from transcriptome data.
Journal of biotechnology 2008;135(3):309-17.
2008: Brinkrolf Karina; Plöger Svenja; Solle Sandra; Brune Iris; Nentwich Svenja S; Hüser Andrea T; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences.
Microbiology (Reading, England) 2008;154(Pt 4):1068-81.
2008: Jungwirth Britta; Emer Denise; Brune Iris; Hansmeier Nicole; Pühler Alfred; Eikmanns Bernhard J; Tauch Andreas
Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR.
FEMS microbiology letters 2008;281(2):190-7.
2008: Meschini Eitel Peltzer; Blanco Flavio Antonio; Zanetti María Eugenia; Beker María Pía; Küster Helge; Pühler Alfred; Aguilar O Mario
Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-rhizobium etli symbiosis revealed by suppressive subtractive hybridization.
Molecular plant-microbe interactions : MPMI 2008;21(4):459-68.
2008: Vorhölter Frank-Jörg; Schneiker Susanne; Goesmann Alexander; Krause Lutz; Bekel Thomas; Kaiser Olaf; Linke Burkhard; Patschkowski Thomas; Rückert Christian; Schmid Joachim; Sidhu Vishaldeep Kaur; Sieber Volker; Tauch Andreas; Watt Steven Alexander; Weisshaar Bernd; Becker Anke; Niehaus Karsten; Pühler Alfred
The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis.
Journal of biotechnology 2008;134(1-2):33-45.
2008: Gartemann Karl-Heinz; Abt Birte; Bekel Thomas; Burger Annette; Engemann Jutta; Flügel Monika; Gaigalat Lars; Goesmann Alexander; Gräfen Ines; Kalinowski Jörn; Kaup Olaf; Kirchner Oliver; Krause Lutz; Linke Burkhard; McHardy Alice; Meyer Folker; Pohle Sandra; Rückert Christian; Schneiker Susanne; Zellermann Eva-Maria; Pühler Alfred; Eichenlaub Rudolf; Kaiser Olaf; Bartels Daniela
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity.
Journal of bacteriology 2008;190(6):2138-49.
2008: Kuhn Stefanie; Stiens Michael; Pühler Alfred; Schlüter Andreas
Prevalence of pSmeSM11a-like plasmids in indigenous Sinorhizobium meliloti strains isolated in the course of a field release experiment with genetically modified S. meliloti strains.
FEMS microbiology ecology 2008;63(1):118-31.
2008: Rückert Christian; Milse Johanna; Albersmeier Andreas; Koch Daniel J; Pühler Alfred; Kalinowski Jörn
The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules.
BMC genomics 2008;9():483.
2007: Rinas U; Pühler A
Merging process engineering and systems biology.
Journal of biotechnology 2007;132(4):341.
2007: Schlüter Andreas; Krahn Irene; Kollin Florian; Bönemann Gabriele; Stiens Michael; Szczepanowski Rafael; Schneiker Susanne; Pühler Alfred
IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes.
Applied and environmental microbiology 2007;73(20):6345-50.
2007: Schlüter Andreas; Szczepanowski Rafael; Pühler Alfred; Top Eva M
Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool.
FEMS microbiology reviews 2007;31(4):449-77.
2007: Nakunst Diana; Larisch Christof; Hüser Andrea T; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes.
Journal of bacteriology 2007;189(13):4696-707.
2007: Stiens Michael; Schneiker Susanne; Pühler Alfred; Schlüter Andreas
Sequence analysis of the 181-kb accessory plasmid pSmeSM11b, isolated from a dominant Sinorhizobium meliloti strain identified during a long-term field release experiment.
FEMS microbiology letters 2007;271(2):297-309.
2007: Hansmeier Nicole; Chao Tzu-Chiao; Daschkey Svenja; Müsken Mathias; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411.
Proteomics 2007;7(7):1076-96.
2007: Brune Iris; Jochmann Nina; Brinkrolf Karina; Hüser Andrea T; Gerstmeir Robert; Eikmanns Bernhard J; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
The IclR-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum.
Journal of bacteriology 2007;189(7):2720-33.
2007: Schlüter A; Szczepanowski R; Kurz N; Schneiker S; Krahn I; Pühler A
Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function.
Applied and environmental microbiology 2007;73(6):1952-60.
2007: Szczepanowski Rafael; Krahn Irene; Bohn Nadine; Pühler Alfred; Schlüter Andreas
Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant.
Antimicrobial agents and chemotherapy 2007;51(2):673-8.
2007: Pühler Alfred; Strack Dieter
Molecular basics of mycorrhizal symbioses.
Phytochemistry 2007;68(1):6-7.
2007: Küster Helge; Becker Anke; Firnhaber Christian; Hohnjec Natalija; Manthey Katja; Perlick Andreas M; Bekel Thomas; Dondrup Michael; Henckel Kolja; Goesmann Alexander; Meyer Folker; Wipf Daniel; Requena Natalia; Hildebrandt Ulrich; Hampp Rüdiger; Nehls Uwe; Krajinski Franziska; Franken Philipp; Pühler Alfred
Development of bioinformatic tools to support EST-sequencing, in silico- and microarray-based transcriptome profiling in mycorrhizal symbioses.
Phytochemistry 2007;68(1):19-32.
2007: Neuweger Heiko; Baumbach Jan; Albaum Stefan; Bekel Thomas; Dondrup Michael; Hüser Andrea T; Kalinowski Jörn; Oehm Sebastian; Pühler Alfred; Rahmann Sven; Weile Jochen; Goesmann Alexander
CoryneCenter - an online resource for the integrated analysis of corynebacterial genome and transcriptome data.
BMC systems biology 2007;1():55.
2007: Gaigalat Lars; Schlüter Jan-Philip; Hartmann Michelle; Mormann Sascha; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum.
BMC molecular biology 2007;8():104.
2007: Krause Lutz; McHardy Alice C; Nattkemper Tim W; Pühler Alfred; Stoye Jens; Meyer Folker
GISMO--gene identification using a support vector machine for ORF classification.
Nucleic acids research 2007;35(2):540-9.
2006: Brune Iris; Becker Anke; Paarmann Daniel; Albersmeier Andreas; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
Under the influence of the active deodorant ingredient 4-hydroxy-3-methoxybenzyl alcohol, the skin bacterium Corynebacterium jeikeium moderately responds with differential gene expression.
Journal of biotechnology 2006;127(1):21-33.
2006: Krause Andrea; Ramakumar Adarsh; Bartels Daniela; Battistoni Federico; Bekel Thomas; Boch Jens; Böhm Melanie; Friedrich Frauke; Hurek Thomas; Krause Lutz; Linke Burkhard; McHardy Alice C; Sarkar Abhijit; Schneiker Susanne; Syed Arshad Ali; Thauer Rudolf; Vorhölter Frank-Jörg; Weidner Stefan; Pühler Alfred; Reinhold-Hurek Barbara; Kaiser Olaf; Goesmann Alexander
Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72.
Nature biotechnology 2006;24(11):1385-91.
2006: Hain Torsten; Steinweg Christiane; Kuenne Carsten Tobias; Billion André; Ghai Rohit; Chatterjee Som Subhra; Domann Eugen; Kärst Uwe; Goesmann Alexander; Bekel Thomas; Bartels Daniela; Kaiser Olaf; Meyer Folker; Pühler Alfred; Weisshaar Bernd; Wehland Jürgen; Liang Chunguang; Dandekar Thomas; Lampidis Robert; Kreft Jürgen; Goebel Werner; Chakraborty Trinad
Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes.
Journal of bacteriology 2006;188(21):7405-15.
2006: Selbitschka W; Keller M; Miethling-Graff R; Dresing U; Schwieger F; Krahn I; Homann I; Dammann-Kalinowski T; Pühler A; Tebbe C C
Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.
Microbial ecology 2006;52(3):583-95.
2006: Bönemann Gabriele; Stiens Michael; Pühler Alfred; Schlüter Andreas
Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant.
Antimicrobial agents and chemotherapy 2006;50(9):3075-80.
2006: Schneiker Susanne; Martins dos Santos Vítor A P; Bartels Daniela; Bekel Thomas; Brecht Martina; Buhrmester Jens; Chernikova Tatyana N; Denaro Renata; Ferrer Manuel; Gertler Christoph; Goesmann Alexander; Golyshina Olga V; Kaminski Filip; Khachane Amit N; Lang Siegmund; Linke Burkhard; McHardy Alice C; Meyer Folker; Nechitaylo Taras; Pühler Alfred; Regenhardt Daniela; Rupp Oliver; Sabirova Julia S; Selbitschka Werner; Yakimov Michail M; Timmis Kenneth N; Vorhölter Frank-Jörg; Weidner Stefan; Kaiser Olaf; Golyshin Peter N
Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis.
Nature biotechnology 2006;24(8):997-1004.
2006: Krause Lutz; Diaz Naryttza N; Bartels Daniela; Edwards Robert A; Pühler Alfred; Rohwer Forest; Meyer Folker; Stoye Jens
Finding novel genes in bacterial communities isolated from the environment.
Bioinformatics (Oxford, England) 2006;22(14):e281-9.
2006: Mahne Martina; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
The Corynebacterium glutamicum gene pmt encoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins.
FEMS microbiology letters 2006;259(2):226-33.
2006: Stiens M; Schneiker S; Keller M; Kuhn S; Pühler A; Schlüter A
Sequence analysis of the 144-kilobase accessory plasmid pSmeSM11a, isolated from a dominant Sinorhizobium meliloti strain identified during a long-term field release experiment.
Applied and environmental microbiology 2006;72(5):3662-72.
2006: Hansmeier Nicole; Albersmeier Andreas; Tauch Andreas; Damberg Thomas; Ros Robert; Anselmetti Dario; Pühler Alfred; Kalinowski Jörn
The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032.
Microbiology (Reading, England) 2006;152(Pt 4):923-35.
2006: Hansmeier Nicole; Chao Tzu-Chiao; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
Mapping and comprehensive analysis of the extracellular and cell surface proteome of the human pathogen Corynebacterium diphtheriae.
Proteomics 2006;6(8):2465-76.
2006: Gaillard Muriel; Vallaeys Tatiana; Vorhölter Frank Jörg; Minoia Marco; Werlen Christoph; Sentchilo Vladimir; Pühler Alfred; van der Meer Jan Roelof
The clc element of Pseudomonas sp. strain B13, a genomic island with various catabolic properties.
Journal of bacteriology 2006;188(5):1999-2013.
2006: Hansmeier Nicole; Chao Tzu-Chiao; Pühler Alfred; Tauch Andreas; Kalinowski Jörn
The cytosolic, cell surface and extracellular proteomes of the biotechnologically important soil bacterium Corynebacterium efficiens YS-314 in comparison to those of Corynebacterium glutamicum ATCC 13032.
Proteomics 2006;6(1):233-50.
2006: Mormann Sascha; Lömker Alexander; Rückert Christian; Gaigalat Lars; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway.
BMC genomics 2006;7():205.
2006: Brune Iris; Werner Hendrikje; Hüser Andrea T; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
The DtxR protein acting as dual transcriptional regulator directs a global regulatory network involved in iron metabolism of Corynebacterium glutamicum.
BMC genomics 2006;7():21.
2005: Thieme Frank; Koebnik Ralf; Bekel Thomas; Berger Carolin; Boch Jens; Büttner Daniela; Caldana Camila; Gaigalat Lars; Goesmann Alexander; Kay Sabine; Kirchner Oliver; Lanz Christa; Linke Burkhard; McHardy Alice C; Meyer Folker; Mittenhuber Gerhard; Nies Dietrich H; Niesbach-Klösgen Ulla; Patschkowski Thomas; Rückert Christian; Rupp Oliver; Schneiker Susanne; Schuster Stephan C; Vorhölter Frank-Jörg; Weber Ernst; Pühler Alfred; Bonas Ulla; Bartels Daniela; Kaiser Olaf
Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence.
Journal of bacteriology 2005;187(21):7254-66.
2005: Koch D J; Rückert C; Rey D A; Mix A; Pühler A; Kalinowski J
Role of the ssu and seu genes of Corynebacterium glutamicum ATCC 13032 in utilization of sulfonates and sulfonate esters as sulfur sources.
Applied and environmental microbiology 2005;71(10):6104-14.
2005: Chao Tzu-Chiao; Buhrmester Jens; Hansmeier Nicole; Pühler Alfred; Weidner Stefan
Role of the regulatory gene rirA in the transcriptional response of Sinorhizobium meliloti to iron limitation.
Applied and environmental microbiology 2005;71(10):5969-82.
2005: Koch Daniel J; Rückert Christian; Albersmeier Andreas; Hüser Andrea T; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate.
Molecular microbiology 2005;58(2):480-94.
2005: Firnhaber Christian; Pühler Alfred; Küster Helge
EST sequencing and time course microarray hybridizations identify more than 700 Medicago truncatula genes with developmental expression regulation in flowers and pods.
Planta 2005;222(2):269-83.
2005: Silberbach Maike; Hüser Andrea; Kalinowski Jörn; Pühler Alfred; Walter Britta; Krämer Reinhard; Burkovski Andreas
DNA microarray analysis of the nitrogen starvation response of Corynebacterium glutamicum.
Journal of biotechnology 2005;119(4):357-67.
2005: Schlüter Andreas; Heuer Holger; Szczepanowski Rafael; Poler Stacey M; Schneiker Susanne; Pühler Alfred; Top Eva M
Plasmid pB8 is closely related to the prototype IncP-1beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein.
Plasmid 2005;54(2):135-48.
2005: Frenzel André; Manthey Katja; Perlick Andreas M; Meyer Folker; Pühler Alfred; Küster Helge; Krajinski Franziska
Combined transcriptome profiling reveals a novel family of arbuscular mycorrhizal-specific Medicago truncatula lectin genes.
Molecular plant-microbe interactions : MPMI 2005;18(8):771-82.
2005: Braun Sebastian G; Meyer Andreas; Holst Otto; Pühler Alfred; Niehaus Karsten
Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells.
Molecular plant-microbe interactions : MPMI 2005;18(7):674-81.
2005: Tauch Andreas; Kaiser Olaf; Hain Torsten; Goesmann Alexander; Weisshaar Bernd; Albersmeier Andreas; Bekel Thomas; Bischoff Nicole; Brune Iris; Chakraborty Trinad; Kalinowski Jörn; Meyer Folker; Rupp Oliver; Schneiker Susanne; Viehoever Prisca; Pühler Alfred
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Journal of bacteriology 2005;187(13):4671-82.
2005: Hüser Andrea T; Chassagnole Christophe; Lindley Nic D; Merkamm Muriel; Guyonvarch Armel; Elisáková Veronika; Pátek Miroslav; Kalinowski Jörn; Brune Iris; Pühler Alfred; Tauch Andreas
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling.
Applied and environmental microbiology 2005;71(6):3255-68.
2005: Silberbach Maike; Schäfer Mathias; Hüser Andrea T; Kalinowski Jörn; Pühler Alfred; Krämer Reinhard; Burkovski Andreas
Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques.
Applied and environmental microbiology 2005;71(5):2391-402.
2005: Rey Daniel A; Nentwich Svenja S; Koch Daniel J; Rückert Christian; Pühler Alfred; Tauch Andreas; Kalinowski Jörn
The McbR repressor modulated by the effector substance S-adenosylhomocysteine controls directly the transcription of a regulon involved in sulphur metabolism of Corynebacterium glutamicum ATCC 13032.
Molecular microbiology 2005;56(4):871-87.
2005: Tennstedt Thomas; Szczepanowski Rafael; Krahn Irene; Pühler Alfred; Schlüter Andreas
Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements.
Plasmid 2005;53(3):218-38.
2005: Szczepanowski R; Braun S; Riedel V; Schneiker S; Krahn I; Pühler A; Schlüter A
The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions.
Microbiology (Reading, England) 2005;151(Pt 4):1095-111.
2005: Hohnjec Natalija; Vieweg Martin F; Pühler Alfred; Becker Anke; Küster Helge
Overlaps in the transcriptional profiles of Medicago truncatula roots inoculated with two different Glomus fungi provide insights into the genetic program activated during arbuscular mycorrhiza.
Plant physiology 2005;137(4):1283-301.
2005: Bartels Daniela; Kespohl Sebastian; Albaum Stefan; Drüke Tanja; Goesmann Alexander; Herold Julia; Kaiser Olaf; Pühler Alfred; Pfeiffer Friedhelm; Raddatz Günter; Stoye Jens; Meyer Folker; Schuster Stephan C
BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison.
Bioinformatics (Oxford, England) 2005;21(7):853-9.
2005: Fehlberg Vera; Vieweg Martin F; Dohmann Esther M N; Hohnjec Natalija; Pühler Alfred; Perlick Andreas M; Küster Helge
The promoter of the leghaemoglobin gene VfLb29: functional analysis and identification of modules necessary for its activation in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots.
Journal of experimental botany 2005;56(413):799-806.
2005: Rückert Christian; Koch Daniel J; Rey Daniel A; Albersmeier Andreas; Mormann Sascha; Pühler Alfred; Kalinowski Jörn
Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction.
BMC genomics 2005;6():121.
2005: Brune Iris; Brinkrolf Karina; Kalinowski Jörn; Pühler Alfred; Tauch Andreas
The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and Corynebacterium jeikeium deduced from the complete genome sequences.
BMC genomics 2005;6(1):86.
2004: Szczepanowski Rafael; Krahn Irene; Pühler Alfred; Schlüter Andreas
Different molecular rearrangements in the integron of the IncP-1 beta resistance plasmid pB10 isolated from a wastewater treatment plant result in elevated beta-lactam resistance levels.
Archives of microbiology 2004;182(6):429-35.
2004: Szczepanowski Rafael; Krahn Irene; Linke Burkhard; Goesmann Alexander; Pühler Alfred; Schlüter Andreas
Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multidrug transport system.
Microbiology (Reading, England) 2004;150(Pt 11):3613-30.
2004: Heuer H; Szczepanowski R; Schneiker S; Pühler A; Top E M; Schlüter A
The complete sequences of plasmids pB2 and pB3 provide evidence for a recent ancestor of the IncP-1beta group without any accessory genes.
Microbiology (Reading, England) 2004;150(Pt 11):3591-9.
2004: Manthey Katja; Krajinski Franziska; Hohnjec Natalija; Firnhaber Christian; Pühler Alfred; Perlick Andreas M; Küster Helge
Transcriptome profiling in root nodules and arbuscular mycorrhiza identifies a collection of novel genes induced during Medicago truncatula root endosymbioses.
Molecular plant-microbe interactions : MPMI 2004;17(10):1063-77.
2004: Hartmann Michael; Barsch Aiko; Niehaus Karsten; Pühler Alfred; Tauch Andreas; Kalinowski Jörn
The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum.
Archives of microbiology 2004;182(4):299-312.
2004: El Yahyaoui Fikri; Küster Helge; Ben Amor Besma; Hohnjec Natalija; Pühler Alfred; Becker Anke; Gouzy Jérôme; Vernié Tatiana; Gough Clare; Niebel Andreas; Godiard Laurence; Gamas Pascal
Expression profiling in Medicago truncatula identifies more than 750 genes differentially expressed during nodulation, including many potential regulators of the symbiotic program.
Plant physiology 2004;136(2):3159-76.
2004: Tauch Andreas; Bischoff Nicole; Pühler Alfred; Kalinowski Jörn
Comparative genomics identified two conserved DNA modules in a corynebacterial plasmid family present in clinical isolates of the opportunistic human pathogen Corynebacterium jeikeium.
Plasmid 2004;52(2):102-18.
2004: Hansmeier Nicole; Bartels Frank W; Ros Robert; Anselmetti Dario; Tauch Andreas; Pühler Alfred; Kalinowski Jörn
Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy.
Journal of biotechnology 2004;112(1-2):177-93.
2004: Kleine H; Wilke R; Pelargus Ch; Rott K; Pühler A; Reiss G; Ros R; Anselmetti D
Absence of intrinsic electric conductivity in single dsDNA molecules.
Journal of biotechnology 2004;112(1-2):91-5.
2004: Brzeska M; Panhorst M; Kamp P B; Schotter J; Reiss G; Pühler A; Becker A; Brückl H
Detection and manipulation of biomolecules by magnetic carriers.
Journal of biotechnology 2004;112(1-2):25-33.
2004: Ros Robert; Eckel Rainer; Bartels Frank; Sischka Andy; Baumgarth Birgit; Wilking Sven David; Pühler Alfred; Sewald Norbert; Becker Anke; Anselmetti Dario
Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications.
Journal of biotechnology 2004;112(1-2):5-12.
2004: Schiene K; Donath S; Brecht M; Pühler A; Niehaus K
A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa.
Molecular genetics and genomics : MGG 2004;272(1):57-66.
2004: McHardy Alice C; Goesmann Alexander; Pühler Alfred; Meyer Folker
Development of joint application strategies for two microbial gene finders.
Bioinformatics (Oxford, England) 2004;20(10):1622-31.
2004: Chao Tzu-Chiao; Becker Anke; Buhrmester Jens; Pühler Alfred; Weidner Stefan
The Sinorhizobium meliloti fur gene regulates, with dependence on Mn(II), transcription of the sitABCD operon, encoding a metal-type transporter.
Journal of bacteriology 2004;186(11):3609-20.
2004: Schotter J; Kamp P B; Becker A; Pühler A; Reiss G; Brückl H
Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection.
Biosensors & bioelectronics 2004;19(10):1149-56.
2004: Pühler Alfred; Arlat Matthieu; Becker Anke; Göttfert Michael; Morrissey John P; O'Gara Fergal
What can bacterial genome research teach us about bacteria-plant interactions?
Current opinion in plant biology 2004;7(2):137-47.
2004: Küster Helge; Hohnjec Natalija; Krajinski Franziska; El Yahyaoui Fikri; Manthey Katja; Gouzy Jéôme; Dondrup Michael; Meyer Folker; Kalinowski Jörn; Brechenmacher Laurent; van Tuinen Diederik; Gianinazzi-Pearson Vivienne; Pühler Alfred; Gamas Pascal; Becker Anke
Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula.
Journal of biotechnology 2004;108(2):95-113.
2004: Becker Anke; Bergès Hélène; Krol Elizaveta; Bruand Claude; Rüberg Silvia; Capela Delphine; Lauber Emmanuelle; Meilhoc Eliane; Ampe Frédéric; de Bruijn Frans J; Fourment Joëlle; Francez-Charlot Anne; Kahn Daniel; Küster Helge; Liebe Carine; Pühler Alfred; Weidner Stefan; Batut Jacques
Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions.
Molecular plant-microbe interactions : MPMI 2004;17(3):292-303.
2004: McHardy Alice C; Pühler Alfred; Kalinowski Jörn; Meyer Folker
Comparing expression level-dependent features in codon usage with protein abundance: an analysis of 'predictive proteomics'.
Proteomics 2004;4(1):46-58.
2004: Vieweg Martin F; Frühling Martin; Quandt Hans-Joachim; Heim Ute; Bäumlein Helmut; Pühler Alfred; Küster Helge; Andreas M Perlick
The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.
Molecular plant-microbe interactions : MPMI 2004;17(1):62-9.
2003: Hüser Andrea T; Becker Anke; Brune Iris; Dondrup Michael; Kalinowski Jörn; Plassmeier Jens; Pühler Alfred; Wiegräbe Iris; Tauch Andreas
Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source.
Journal of biotechnology 2003;106(2-3):269-86.
2003: Rüberg Silvia; Tian Zhe-Xian; Krol Elizaveta; Linke Burkhard; Meyer Folker; Wang Yiping; Pühler Alfred; Weidner Stefan; Becker Anke
Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: genome-wide profiling of osmoadaptive gene expression.
Journal of biotechnology 2003;106(2-3):255-68.
2003: Golyshin Peter N; Martins Dos Santos Vitor A P; Kaiser Olaf; Ferrer Manuel; Sabirova Yulia S; Lünsdorf H; Chernikova Tatyana N; Golyshina Olga V; Yakimov Michail M; Pühler Alfred; Timmis Kenneth N
Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems.
Journal of biotechnology 2003;106(2-3):215-20.
2003: Vorhölter F-J; Thias T; Meyer F; Bekel T; Kaiser O; Pühler A; Niehaus K
Comparison of two Xanthomonas campestris pathovar campestris genomes revealed differences in their gene composition.
Journal of biotechnology 2003;106(2-3):193-202.
2003: Goesmann Alexander; Linke Burkhard; Rupp Oliver; Krause Lutz; Bartels Daniela; Dondrup Michael; McHardy Alice C; Wilke Andreas; Pühler Alfred; Meyer Folker
Building a BRIDGE for the integration of heterogeneous data from functional genomics into a platform for systems biology.
Journal of biotechnology 2003;106(2-3):157-67.
2003: Wilke Andreas; Rückert Christian; Bartels Daniela; Dondrup Michael; Goesmann Alexander; Hüser Andrea T; Kespohl Sebastian; Linke Burkhard; Mahne Martina; McHardy Alice; Pühler Alfred; Meyer Folker
Bioinformatics support for high-throughput proteomics.
Journal of biotechnology 2003;106(2-3):147-56.
2003: Dondrup Michael; Goesmann Alexander; Bartels Daniela; Kalinowski Jörn; Krause Lutz; Linke Burkhard; Rupp Oliver; Sczyrba Alexander; Pühler Alfred; Meyer Folker
EMMA: a platform for consistent storage and efficient analysis of microarray data.
Journal of biotechnology 2003;106(2-3):135-46.
2003: Kaiser Olaf; Bartels Daniela; Bekel Thomas; Goesmann Alexander; Kespohl Sebastian; Pühler Alfred; Meyer Folker
Whole genome shotgun sequencing guided by bioinformatics pipelines--an optimized approach for an established technique.
Journal of biotechnology 2003;106(2-3):121-33.
2003: Schlüter A; Heuer H; Szczepanowski R; Forney L J; Thomas C M; Pühler A; Top E M
The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group.
Microbiology (Reading, England) 2003;149(Pt 11):3139-53.
2003: Hohnjec Natalija; Perlick Andreas M; Pühler Alfred; Küster Helge
The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi.
Molecular plant-microbe interactions : MPMI 2003;16(10):903-15.
2003: McHardy Alice C; Tauch Andreas; Rückert Christian; Pühler Alfred; Kalinowski Jörn
Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum.
Journal of biotechnology 2003;104(1-3):229-40.
2003: Rückert C; Pühler A; Kalinowski J
Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation.
Journal of biotechnology 2003;104(1-3):213-28.
2003: Hartmann Michael; Tauch Andreas; Eggeling Lothar; Bathe Brigitte; Möckel Bettina; Pühler Alfred; Kalinowski Jörn
Identification and characterization of the last two unknown genes, dapC and dapF, in the succinylase branch of the L-lysine biosynthesis of Corynebacterium glutamicum.
Journal of biotechnology 2003;104(1-3):199-211.
2003: Tauch Andreas; Pühler Alfred; Kalinowski Jörn; Thierbach Georg
Plasmids in Corynebacterium glutamicum and their molecular classification by comparative genomics.
Journal of biotechnology 2003;104(1-3):27-40.
2003: Kalinowski Jörn; Bathe Brigitte; Bartels Daniela; Bischoff Nicole; Bott Michael; Burkovski Andreas; Dusch Nicole; Eggeling Lothar; Eikmanns Bernhard J; Gaigalat Lars; Goesmann Alexander; Hartmann Michael; Huthmacher Klaus; Krämer Reinhard; Linke Burkhard; McHardy Alice C; Meyer Folker; Möckel Bettina; Pfefferle Walter; Pühler Alfred; Rey Daniel A; Rückert Christian; Rupp Oliver; Sahm Hermann; Wendisch Volker F; Wiegräbe Iris; Tauch Andreas
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Journal of biotechnology 2003;104(1-3):5-25.
2003: Tennstedt Thomas; Szczepanowski Rafael; Braun Sebastian; Pühler Alfred; Schlüter Andreas
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
FEMS microbiology ecology 2003;45(3):239-52.
2003: Brand Sven; Niehaus Karsten; Pühler Alfred; Kalinowski Jörn
Identification and functional analysis of six mycolyltransferase genes of Corynebacterium glutamicum ATCC 13032: the genes cop1, cmt1, and cmt2 can replace each other in the synthesis of trehalose dicorynomycolate, a component of the mycolic acid layer of the cell envelope.
Archives of microbiology 2003;180(1):33-44.
2003: Rey Daniel Alexander; Pühler Alfred; Kalinowski Jörn
The putative transcriptional repressor McbR, member of the TetR-family, is involved in the regulation of the metabolic network directing the synthesis of sulfur containing amino acids in Corynebacterium glutamicum.
Journal of biotechnology 2003;103(1):51-65.
2003: Weidner Stefan; Pühler Alfred; Küster Helge
Genomics insights into symbiotic nitrogen fixation.
Current opinion in biotechnology 2003;14(2):200-5.
2003: Meyer Folker; Goesmann Alexander; McHardy Alice C; Bartels Daniela; Bekel Thomas; Clausen Jörn; Kalinowski Jörn; Linke Burkhard; Rupp Oliver; Giegerich Robert; Pühler Alfred
GenDB--an open source genome annotation system for prokaryote genomes.
Nucleic acids research 2003;31(8):2187-95.
2003: Tauch A; Schlüter A; Bischoff N; Goesmann A; Meyer F; Pühler A
The 79,370-bp conjugative plasmid pB4 consists of an IncP-1beta backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene bla(NPS-1), and a tripartite antibiotic efflux system of the resistance-nodulation-division family.
Molecular genetics and genomics : MGG 2003;268(5):570-84.
2002: Tauch Andreas; Kirchner Oliver; Löffler Britta; Götker Susanne; Pühler Alfred; Kalinowski Jörn
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Current microbiology 2002;45(5):362-7.
2002: Tauch Andreas; Götker Susanne; Pühler Alfred; Kalinowski Jörn; Thierbach Georg
The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains.
Journal of biotechnology 2002;99(1):79-91.
2002: Tauch Andreas; Götker Susanne; Pühler Alfred; Kalinowski Jörn; Thierbach Georg
The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100.
Plasmid 2002;48(2):117-29.
2002: Pühler Alfred
Highlights from ECB10-Biotechnology and Society.
Journal of biotechnology 2002;98(1):1-2.
2002: Tauch Andreas; Schneiker Susanne; Selbitschka Werner; Pühler Alfred; van Overbeek Leo S; Smalla Kornelia; Thomas Christopher M; Bailey Mark J; Forney Larry J; Weightman Andrew; Ceglowski Piotr; Pembroke Tony; Tietze Erhard; Schröder Gunnar; Lanka Erich; van Elsas Jan Dirk
The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere.
Microbiology (Reading, England) 2002;148(Pt 6):1637-53.
2002: Claes Wilfried A; Pühler Alfred; Kalinowski Jörn
Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle.
Journal of bacteriology 2002;184(10):2728-39.
2002: Becker Anke; Rüberg Silvia; Baumgarth Birgit; Bertram-Drogatz Peter Alexander; Quester Ingmar; Pühler Alfred
Regulation of succinoglycan and galactoglucan biosynthesis in Sinorhizobium meliloti.
Journal of molecular microbiology and biotechnology 2002;4(3):187-90.
2002: Tauch Andreas; Homann Iris; Mormann Sascha; Rüberg Silvia; Billault Alain; Bathe Brigitte; Brand Sven; Brockmann-Gretza Olaf; Rückert Christian; Schischka Natalie; Wrenger Carsten; Hoheisel Jörg; Möckel Bettina; Huthmacher Klaus; Pfefferle Walter; Pühler Alfred; Kalinowski Jörn
Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library.
Journal of biotechnology 2002;95(1):25-38.
2001: Schneiker S; Keller M; Dröge M; Lanka E; Pühler A; Selbitschka W
The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa.
Nucleic acids research 2001;29(24):5169-81.
2001: Becker J D; Moreira L M; Kapp D; Frosch S C; Pühler A; Perlic A M
The nodulin vfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules.
Plant molecular biology 2001;47(6):749-59.
2001: Vorhölter F J; Niehaus K; Pühler A
Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core.
Molecular genetics and genomics : MGG 2001;266(1):79-95.
2001: Finan T M; Weidner S; Wong K; Buhrmester J; Chain P; Vorhölter F J; Hernandez-Lucas I; Becker A; Cowie A; Gouzy J; Golding B; Pühler A
The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(17):9889-94.
2001: Capela D; Barloy-Hubler F; Gouzy J; Bothe G; Ampe F; Batut J; Boistard P; Becker A; Boutry M; Cadieu E; Dréano S; Gloux S; Godrie T; Goffeau A; Kahn D; Kiss E; Lelaure V; Masuy D; Pohl T; Portetelle D; Pühler A; Purnelle B; Ramsperger U; Renard C; Thébault P; Vandenbol M; Weidner S; Galibert F
Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(17):9877-82.
2001: Tauch A; Wehmeier L; Götker S; Pühler A; Kalinowski J
Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism.
FEMS microbiology letters 2001;201(1):53-8.
2001: Schröter K; Flaschel E; Pühler A; Becker A
Xanthomonas campestris pv. campestris secretes the endoglucanases ENGXCA and ENGXCB: construction of an endoglucanase-deficient mutant for industrial xanthan production.
Applied microbiology and biotechnology 2001;55(6):727-33.
2001: Meyer A; Pühler A; Niehaus K
The lipopolysaccharides of the phytopathogen Xanthomonas campestris pv. campestris induce an oxidative burst reaction in cell cultures of Nicotiana tabacum.
Planta 2001;213(2):214-22.
2001: Hermann T; Pfefferle W; Baumann C; Busker E; Schaffer S; Bott M; Sahm H; Dusch N; Kalinowski J; Pühler A; Bendt A K; Krämer R; Burkovski A
Proteome analysis of Corynebacterium glutamicum.
Electrophoresis 2001;22(9):1712-23.
2001: Wehmeier L; Brockmann-Gretza O; Pisabarro A; Tauch A; Pühler A; Martin J F; Kalinowski J
A Corynebacterium glutamicum mutant with a defined deletion within the rplK gene is impaired in (p)ppGpp accumulation upon amino acid starvation.
Microbiology (Reading, England) 2001;147(Pt 3):691-700.
2000: Tauch A; Pühler A; Kalinowski J; Thierbach G
TetZ, a new tetracycline resistance determinant discovered in gram-positive bacteria, shows high homology to gram-negative regulated efflux systems.
Plasmid 2000;44(3):285-91.
2000: Hohnjec N; Küster H; Albus U; Frosch S C; Becker J D; Pühler A; Perlick A M; Frühling M
The broad bean nodulin VfENOD18 is a member of a novel family of plant proteins with homologies to the bacterial MJ0577 superfamily.
Molecular & general genetics : MGG 2000;264(3):241-50.
2000: Moreira L M; Becker J D; Pühler A; Becker A
The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan.
Microbiology (Reading, England) 2000;146 ( Pt 9)():2237-48.
2000: Schiene K; Pühler A; Niehaus K
Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltration.
Molecular & general genetics : MGG 2000;263(5):761-70.
2000: Wiggerich H G; Pühler A
The exbD2 gene as well as the iron-uptake genes tonB, exbB and exbD1 of Xanthomonas campestris pv. campestris are essential for the induction of a hypersensitive response on pepper (Capsicum annuum).
Microbiology (Reading, England) 2000;146 ( Pt 5)():1053-60.
2000: Dröge M; Pühler A; Selbitschka W
Phenotypic and molecular characterization of conjugative antibiotic resistance plasmids isolated from bacterial communities of activated sludge.
Molecular & general genetics : MGG 2000;263(3):471-82.
2000: Tauch A; Krieft S; Kalinowski J; Pühler A
The 51,409-bp R-plasmid pTP10 from the multiresistant clinical isolate Corynebacterium striatum M82B is composed of DNA segments initially identified in soil bacteria and in plant, animal, and human pathogens.
Molecular & general genetics : MGG 2000;263(1):1-11.
1999: Schneiker S; Kosier B; Pühler A; Selbitschka W
The Sinorhizobium meliloti insertion sequence (IS) element ISRm14 is related to a previously unrecognized IS element located adjacent to the Escherichia coli locus of enterocyte effacement (LEE) pathogenicity island.
Current microbiology 1999;39(5):274-81.
1999: Quast K; Bathe B; Pühler A; Kalinowski J
The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism.
Molecular & general genetics : MGG 1999;262(3):568-78.
1999: Elischewski F; Pühler A; Kalinowski J
Pantothenate production in Escherichia coli K12 by enhanced expression of the panE gene encoding ketopantoate reductase.
Journal of biotechnology 1999;75(2-3):135-46.
1999: Niemann S; Dammann-Kalinowski T; Nagel A; Pühler A; Selbitschka W
Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S. meliloti employing PCR primers derived from an ERIC-PCR fragment.
Archives of microbiology 1999;172(1):22-30.
1999: Michel K P; Krüger F; Pühler A; Pistorius E K
Molecular characterization of idiA and adjacent genes in the cyanobacteria Synechococcus sp. strains PCC 6301 and PCC 7942.
Microbiology (Reading, England) 1999;145 ( Pt 6)():1473-84.
1999: Hohnjec N; Becker J D; Pühler A; Perlick A M; Küster H
Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula.
Molecular & general genetics : MGG 1999;261(3):514-22.
1999: Tauch A; Krieft S; Pühler A; Kalinowski J
The tetAB genes of the Corynebacterium striatum R-plasmid pTP10 encode an ABC transporter and confer tetracycline, oxytetracycline and oxacillin resistance in Corynebacterium glutamicum.
FEMS microbiology letters 1999;173(1):203-9.
1999: Dusch N; Pühler A; Kalinowski J
Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli.
Applied and environmental microbiology 1999;65(4):1530-9.
1999: Rüberg S; Pühler A; Becker A
Biosynthesis of the exopolysaccharide galactoglucan in Sinorhizobium meliloti is subject to a complex control by the phosphate-dependent regulator PhoB and the proteins ExpG and MucR.
Microbiology (Reading, England) 1999;145 ( Pt 3)():603-11.
1999: Selbitschka W; Zekri S; Schröder G; Pühler A; Toro N
The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements.
FEMS microbiology letters 1999;172(1):1-7.
1998: Dröge M; Pühler A; Selbitschka W
Horizontal gene transfer as a biosafety issue: a natural phenomenon of public concern.
Journal of biotechnology 1998;64(1):75-90.
1998: Tauch A; Zheng Z; Pühler A; Kalinowski J
Corynebacterium striatum chloramphenicol resistance transposon Tn5564: genetic organization and transposition in Corynebacterium glutamicum.
Plasmid 1998;40(2):126-39.
1998: Becker A; Katzen F; Pühler A; Ielpi L
Xanthan gum biosynthesis and application: a biochemical/genetic perspective.
Applied microbiology and biotechnology 1998;50(2):145-52.
1998: Wehmeier L; Schäfer A; Burkovski A; Krämer R; Mechold U; Malke H; Pühler A; Kalinowski J
The role of the Corynebacterium glutamicum rel gene in (p)ppGpp metabolism.
Microbiology (Reading, England) 1998;144 ( Pt 7)():1853-62.
1998: Katzen F; Ferreiro D U; Oddo C G; Ielmini M V; Becker A; Pühler A; Ielpi L
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.
Journal of bacteriology 1998;180(7):1607-17.
1998: Tauch A; Hermann T; Burkovski A; Krämer R; Pühler A; Kalinowski J
Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the brnQ gene product.
Archives of microbiology 1998;169(4):303-12.
1998: Bertram-Drogatz P A; Quester I; Becker A; Pühler A
The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY.
Molecular & general genetics : MGG 1998;257(4):433-41.
1998: Becker A; Pühler A
Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan.
Journal of bacteriology 1998;180(2):395-9.
1997: Kohring B; Baier R; Niehaus K; Pühler A; Flaschel E
Production of nodulation factors by Rhizobium meliloti: fermentation, purification and characterization of glycolipids.
Glycoconjugate journal 1997;14(8):963-71.
1997: Schäfer A; Tauch A; Droste N; Pühler A; Kalinowski J
The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine methyltransferase enzyme confers a specific DNA methylation pattern in an McrBC-deficient Escherichia coli strain.
Gene 1997;203(2):95-101.
1997: Steinmann D; Köplin R; Pühler A; Niehaus K
Xanthomonas campestris pv. campestris lpsI and lpsJ genes encoding putative proteins with sequence similarity to the alpha- and beta-subunits of 3-oxoacid CoA-transferases are involved in LPS biosynthesis.
Archives of microbiology 1997;168(6):441-7.
1997: Wiggerich H G; Klauke B; Köplin R; Priefer U B; Pühler A
Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not.
Journal of bacteriology 1997;179(22):7103-10.
1997: Uhde C; Schmidt R; Jording D; Selbitschka W; Pühler A
Stationary-phase mutants of Sinorhizobium meliloti are impaired in stationary-phase survival or in recovery to logarithmic growth.
Journal of bacteriology 1997;179(20):6432-40.
1997: Bertram-Drogatz P A; Rüberg S; Becker A; Pühler A
The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti.
Molecular & general genetics : MGG 1997;254(5):529-38.
1997: Niemann S; Pühler A; Tichy H V; Simon R; Selbitschka W
Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population.
Journal of applied microbiology 1997;82(4):477-84.
1997: Jäger W; Kalinowski J; Pühler A
A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.
Journal of bacteriology 1997;179(7):2449-51.
1997: Becker A; Rüberg S; Küster H; Roxlau A A; Keller M; Ivashina T; Cheng H P; Walker G C; Pühler A
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Journal of bacteriology 1997;179(4):1375-84.
1997: Frühling M; Roussel H; Gianinazzi-Pearson V; Pühler A; Perlick A M
The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum.
Molecular plant-microbe interactions : MPMI 1997;10(1):124-31.
1996: Fernández-González C; Gil J A; Mateos L M; Schwarzer A; Schäfer A; Kalinowski J; Pühler A; Martín J F
Construction of L-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes.
Applied microbiology and biotechnology 1996;46(5-6):554-8.
1996: Perlick A M; Albus U; Stavridis T; Frühling M; Küster H; Pühler A
The Vicia faba lipoxygenase gene VfLOX1 is expressed in the root nodule parenchyma.
Molecular plant-microbe interactions : MPMI 1996;9(9):860-3.
1996: Küster H; Frühling M; Pühler A; Perlick A M
The modular nodulins Nvf-28/32 of broad bean (Vicia faba L.): alternative exon combinations account for different modular structures.
Molecular & general genetics : MGG 1996;252(6):648-57.
1996: Mateos L M; Schäfer A; Kalinowski J; Martin J F; Pühler A
Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation.
Journal of bacteriology 1996;178(19):5768-75.
1996: Bathe B; Kalinowski J; Pühler A
A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.
Molecular & general genetics : MGG 1996;252(3):255-65.
1996: Jäger W; Peters-Wendisch P G; Kalinowski J; Pühler A
A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins.
Archives of microbiology 1996;166(2):76-82.
1996: Katzen F; Becker A; Zorreguieta A; Pühler A; Ielpi L
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
Journal of bacteriology 1996;178(14):4313-8.
1996: Kriete G; Niehaus K; Perlick A M; Pühler A; Broer I
Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin.
The Plant journal : for cell and molecular biology 1996;9(6):809-18.
1996: Dammann-Kalinowski T; Niemann S; Keller M; Selbitschka W; Tebbe C C; Pühler A
Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases.
Applied microbiology and biotechnology 1996;45(4):509-12.
1996: Perlick A M; Frühling M; Schröder G; Frosch S C; Pühler A
The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)8-barrel-type seed proteins.
Plant physiology 1996;110(1):147-54.
1995: Becker A; Küster H; Niehaus K; Pühler A
Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis.
Molecular & general genetics : MGG 1995;249(5):487-97.
1995: Küster H; Quandt H J; Broer I; Perlick A M; Pühler A
The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules.
Plant molecular biology 1995;29(4):759-72.
1995: Tauch A; Kassing F; Kalinowski J; Pühler A
The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX.
Plasmid 1995;34(2):119-31.
1995: Selbitschka W; Arnold W; Jording D; Kosier B; Toro N; Pühler A
The insertion sequence element ISRm2011-2 belongs to the IS630-Tc1 family of transposable elements and is abundant in Rhizobium meliloti.
Gene 1995;163(1):59-64.
1995: Riedel K U; Jouanneau Y; Masepohl B; Pühler A; Klipp W
A Rhizobium meliloti ferredoxin (FdxN) purified from Escherichia coli donates electrons to Rhodobacter capsulatus nitrogenase.
European journal of biochemistry / FEBS 1995;231(3):742-6.
1995: Becker A; Schmidt M; Jäger W; Pühler A
New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.
Gene 1995;162(1):37-9.
1995: Küster H; Schröder G; Frühling M; Pich U; Rieping M; Schubert I; Perlick A M; Pühler A
The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules.
Plant molecular biology 1995;28(3):405-21.
1995: Selbitschka W; Jording D; Nieman S; Schmidt R; Pühler A; Mendum T; Hirsch P
Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment.
FEMS microbiology letters 1995;128(3):255-63.
1995: Tauch A; Kassing F; Kalinowski J; Pühler A
The erythromycin resistance gene of the Corynebacterium xerosis R-plasmid pTP10 also carrying chloramphenicol, kanamycin, and tetracycline resistances is capable of transposition in Corynebacterium glutamicum.
Plasmid 1995;33(3):168-79.
1995: Becker A; Niehaus K; Pühler A
Low-molecular-weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal domain and a missing C-terminal domain.
Molecular microbiology 1995;16(2):191-203.
1995: Keller M; Roxlau A; Weng W M; Schmidt M; Quandt J; Niehaus K; Jording D; Arnold W; Pühler A
Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharides succinoglycan and galactoglucan.
Molecular plant-microbe interactions : MPMI 1995;8(2):267-77.
1995: Jäger W; Schäfer A; Kalinowski J; Pühler A
Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker.
FEMS microbiology letters 1995;126(1):1-6.
1995: Broer I; Dröge-Laser W; Barker R F; Neumann K; Klipp W; Pühler A
Identification of the Agrobacterium tumefaciens C58 T-DNA genes e and f and their impact on crown gall tumour formation.
Plant molecular biology 1995;27(1):41-57.
1994: Schäfer A; Schwarzer A; Kalinowski J; Pühler A
Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli.
Journal of bacteriology 1994;176(23):7309-19.
1994: Tauch A; Kirchner O; Wehmeier L; Kalinowski J; Pühler A
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
FEMS microbiology letters 1994;123(3):343-7.
1994: Schäfer A; Tauch A; Jäger W; Kalinowski J; Thierbach G; Pühler A
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
Gene 1994;145(1):69-73.
1994: Miksch G; Arnold W; Lentzsch P; Priefer U B; Pühler A
A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureA, ureB, ureC) interrupted by other open reading frames.
Molecular & general genetics : MGG 1994;242(5):539-50.
1994: Schäfer A; Kalinowski J; Pühler A
Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure.
Applied and environmental microbiology 1994;60(2):756-9.
1994: Küster H; Perlick A M; Pühler A
Members of a broadbean nodulin family with partial homologies to the alfalfa nodulin 25 are composed of two types of amino acid repeats flanked by unique amino acid sequence termini.
Plant molecular biology 1994;24(1):143-57.
1993: Köplin R; Wang G; Hötte B; Priefer U B; Pühler A
A 3.9-kb DNA region of Xanthomonas campestris pv. campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose.
Journal of bacteriology 1993;175(24):7786-92.
1993: Becker A; Kleickmann A; Keller M; Arnold W; Pühler A
Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment.
Molecular & general genetics : MGG 1993;241(3-4):367-79.
1993: Becker A; Kleickmann A; Küster H; Keller M; Arnold W; Pühler A
Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT, and exoI involved in exopolysaccharide biosynthesis and nodule invasion: exoU and exoW probably encode glucosyltransferases.
Molecular plant-microbe interactions : MPMI 1993;6(6):735-44.
1993: Steinmann D; Wiggerich H G; Klauke B; Schramm U; Pühler A; Priefer U B
Saturation mutagenesis in Escherichia coli of a cloned Xanthomonas campestris DNA fragment with the lux transposon Tn4431 using the delivery plasmid pDS1, thermosensitive in replication.
Applied microbiology and biotechnology 1993;40(2-3):356-60.
1993: Jording D; Pühler A
The membrane topology of the Rhizobium meliloti C4-dicarboxylate permease (DctA) as derived from protein fusions with Escherichia coli K12 alkaline phosphatase (PhoA) and beta-galactosidase (LacZ).
Molecular & general genetics : MGG 1993;241(1-2):106-14.
1993: Perlick A M; Pühler A
A survey of transcripts expressed specifically in root nodules of broadbean (Vicia faba L.).
Plant molecular biology 1993;22(6):957-70.
1993: Küster H; Frühling M; Perlick A M; Pühler A
The sucrose synthase gene is predominantly expressed in the root nodule tissue of Vicia faba.
Molecular plant-microbe interactions : MPMI 1993;6(4):507-14.
1993: Becker A; Kleickmann A; Arnold W; Pühler A
Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins.
Molecular & general genetics : MGG 1993;238(1-2):145-54.
1993: Müller P; Keller M; Weng W M; Quandt J; Arnold W; Pühler A
Genetic analysis of the Rhizobium meliloti exoYFQ operon: ExoY is homologous to sugar transferases and ExoQ represents a transmembrane protein.
Molecular plant-microbe interactions : MPMI 1993;6(1):55-65.
1992: Schmidt M; Arnold W; Niemann A; Kleickmann A; Pühler A
The Rhizobium meliloti pmi gene encodes a new type of phosphomannose isomerase.
Gene 1992;122(1):35-43.
1992: Walter C; Broer I; Hillemann D; Pühler A
High frequency, heat treatment-induced inactivation of the phosphinothricin resistance gene in transgenic single cell suspension cultures of Medicago sativa.
Molecular & general genetics : MGG 1992;235(2-3):189-96.
1992: Krey R; Pühler A; Klipp W
A defined amino acid exchange close to the putative nucleotide binding site is responsible for an oxygen-tolerant variant of the Rhizobium meliloti NifA protein.
Molecular & general genetics : MGG 1992;234(3):433-41.
1992: Jäger W; Schäfer A; Pühler A; Labes G; Wohlleben W
Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.
Journal of bacteriology 1992;174(16):5462-5.
1992: Riedel K U; Masepohl B; Klipp W; Pühler A
Synthesis of the ferredoxin-like protein FdxN from Rhizobium meliloti bacteroids as a fusion protein in Escherichia coli.
Canadian journal of microbiology 1992;38(6):534-40.
1992: Masepohl B; Kutsche M; Riedel K U; Schmehl M; Klipp W; Pühler A
Functional analysis of the cysteine motifs in the ferredoxin-like protein FdxN of Rhizobium meliloti involved in symbiotic nitrogen fixation.
Molecular & general genetics : MGG 1992;233(1-2):33-41.
1992: Rossol I; Pühler A
The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine.
Journal of bacteriology 1992;174(9):2968-77.
1992: Köplin R; Arnold W; Hötte B; Simon R; Wang G; Pühler A
Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis.
Journal of bacteriology 1992;174(1):191-9.
1991: Seep-Feldhaus A H; Kalinowski J; Pühler A
Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake.
Molecular microbiology 1991;5(12):2995-3005.
1991: Sonnen H; Thierbach G; Kautz S; Kalinowski J; Schneider J; Pühler A; Kutzner H J
Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6.
Gene 1991;107(1):69-74.
1991: Selbitschka W; Arnold W; Priefer U B; Rottschäfer T; Schmidt M; Simon R; Pühler A
Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae.
Molecular & general genetics : MGG 1991;229(1):86-95.
1991: Schrumpf B; Schwarzer A; Kalinowski J; Pühler A; Eggeling L; Sahm H
A functionally split pathway for lysine synthesis in Corynebacterium glutamicium.
Journal of bacteriology 1991;173(14):4510-6.
1991: Buendia A M; Enenkel B; Köplin R; Niehaus K; Arnold W; Pühler A
The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM.
Molecular microbiology 1991;5(6):1519-30.
1991: Kalinowski J; Cremer J; Bachmann B; Eggeling L; Sahm H; Pühler A
Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum.
Molecular microbiology 1991;5(5):1197-204.
1991: Alijah R; Dorendorf J; Talay S; Pühler A; Wohlleben W
Genetic analysis of the phosphinothricin-tripeptide biosynthetic pathway of Streptomyces viridochromogenes Tü494.
Applied microbiology and biotechnology 1991;34(6):749-55.
1991: Hillemann D; Pühler A; Wohlleben W
Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors.
Nucleic acids research 1991;19(4):727-31.
1991: Schwarzer A; Pühler A
Manipulation of Corynebacterium glutamicum by gene disruption and replacement.
Bio/technology (Nature Publishing Company) 1991;9(1):84-7.
1990: Kalinowski J; Bachmann B; Thierbach G; Pühler A
Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspartate beta-semialdehyde dehydrogenase gene asd in Corynebacterium glutamicum.
Molecular & general genetics : MGG 1990;224(3):317-24.
1990: Behrmann I; Hillemann D; Pühler A; Strauch E; Wohlleben W
Overexpression of a Streptomyces viridochromogenes gene (glnII) encoding a glutamine synthetase similar to those of eucaryotes confers resistance against the antibiotic phosphinothricyl-alanyl-alanine.
Journal of bacteriology 1990;172(9):5326-34.
1990: Colonna-Romano S; Arnold W; Schlüter A; Boistard P; Pühler A; Priefer U B
An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.
Molecular & general genetics : MGG 1990;223(1):138-47.
1990: Hötte B; Rath-Arnold I; Pühler A; Simon R
Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
Journal of bacteriology 1990;172(5):2804-7.
1990: Labes M; Pühler A; Simon R
A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria.
Gene 1990;89(1):37-46.
1990: Schäfer A; Kalinowski J; Simon R; Seep-Feldhaus A H; Pühler A
High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.
Journal of bacteriology 1990;172(3):1663-6.
1990: Thierbach G; Kalinowski J; Bachmann B; Pühler A
Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
Applied microbiology and biotechnology 1990;32(4):443-8.
1989: Engelke T; Jording D; Kapp D; Pühler A
Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier.
Journal of bacteriology 1989;171(10):5551-60.
1989: Moreno-Vivian C; Hennecke S; Pühler A; Klipp W
Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus.
Journal of bacteriology 1989;171(5):2591-8.
1989: Klipp W; Reiländer H; Schlüter A; Krey R; Pühler A
The Rhizobium meliloti fdxN gene encoding a ferredoxin-like protein is necessary for nitrogen fixation and is cotranscribed with nifA and nifB.
Molecular & general genetics : MGG 1989;216(2-3):293-302.
1989: Priefer U B; Kalinowski J; Rüger B; Heumann W; Pühler A
ISR1, a transposable DNA sequence resident in Rhizobium class IV strains, shows structural characteristics of classical insertion elements.
Plasmid 1989;21(2):120-8.
1988: Wohlleben W; Arnold W; Broer I; Hillemann D; Strauch E; Pühler A
Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum.
Gene 1988;70(1):25-37.
1988: Arnold W; Rump A; Klipp W; Priefer U B; Pühler A
Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae.
Journal of molecular biology 1988;203(3):715-38.
1988: Arnold W; Pühler A
A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing.
Gene 1988;70(1):171-9.
1988: Masepohl B; Klipp W; Pühler A
Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus.
Molecular & general genetics : MGG 1988;212(1):27-37.
1988: Klipp W; Masepohl B; Pühler A
Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region.
Journal of bacteriology 1988;170(2):693-9.
1988: Strauch E; Wohlleben W; Pühler A
Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli.
Gene 1988;63(1):65-74.
1987: Aguilar O M; Reiländer H; Arnold W; Pühler A
Rhizobium meliloti nifN (fixF) gene is part of an operon regulated by a nifA-dependent promoter and codes for a polypeptide homologous to the nifK gene product.
Journal of bacteriology 1987;169(12):5393-400.
1987: Wohlleben W; Pühler A
The Streptomyces ghanaensis low copy plasmid pSG2 and its use for vector construction.
Archives of microbiology 1987;148(4):298-304.
1987: Colonna-Romano S; Riccio A; Guida M; Defez R; Lamberti A; Iaccarino M; Arnold W; Priefer U; Pühler A
Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum.
Nucleic acids research 1987;15(5):1951-64.
1987: Strauch E; Wohlleben W; Pühler A
Development of a plasmid-cloning system for Streptomyces viridochromogenes Tü494.
Journal of basic microbiology 1987;27(8):449-55.
1981: Priefer U B; Burkardt H J; Klipp W; Pühler A
ISR1: an insertion element isolated from the soil bacterium Rhizobium lupini.
Cold Spring Harbor symposia on quantitative biology 1981;45 Pt 1():87-91.

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