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Cristina Purcarea

Research Profile

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Elena Popa; David R Evans; Hedeel Guy-Evans; Csaba Z Kibédi-Szabó; Nirosha Perera; Cristina Purcarea
The smallest active carbamoyl phosphate synthetase was identified in the human gut archaeon Methanobrevibacter smithii.
Pengfei Zhang; Philip D Martin; Cristina Purcarea; Asmita Vaishnav; Joseph S. Brunzelle; Roshini Fernando; Hedeel I Guy-Evans; David R Evans; Brian F P Edwards
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.
Cristina Purcarea; Roshini Fernando; Hedeel Guy Evans; David R Evans
The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis.

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All Publications

2012: Elena Popa; David R Evans; Hedeel Guy-Evans; Csaba Z Kibédi-Szabó; Nirosha Perera; Cristina Purcarea
The smallest active carbamoyl phosphate synthetase was identified in the human gut archaeon Methanobrevibacter smithii.
Journal of molecular microbiology and biotechnology 2012;22(5):287-99.
2009: Pengfei Zhang; Philip D Martin; Cristina Purcarea; Asmita Vaishnav; Joseph S. Brunzelle; Roshini Fernando; Hedeel I Guy-Evans; David R Evans; Brian F P Edwards
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.
Biochemistry 2009;48(4):766-78.
2008: Cristina Purcarea; Roshini Fernando; Hedeel Guy Evans; David R Evans
The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis.
Molecular and cellular biochemistry 2008;311(1-2):199-213.
2005: Philip D Martin; Cristina Purcarea; Pengfei Zhang; Asmita Vaishnav; Sharon Sadecki; Hedeel I Guy-Evans; David R Evans; Brian F P Edwards
The crystal structure of a novel, latent dihydroorotase from Aquifex aeolicus at 1.7A resolution.
Journal of molecular biology 2005;348(3):535-47.
2005: Michael Kothe; Cristina Purcarea; Hedeel I Guy; David R Evans; Susan G Powers-Lee
Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase.
Protein science : a publication of the Protein Society 2005;14(1):37-44.
2004: Anupama Ahuja; Cristina Purcarea; Richard Ebert; Sharon Sadecki; Hedeel I Guy; David R Evans
Aquifex aeolicus dihydroorotase: association with aspartate transcarbamoylase switches on catalytic activity.
The Journal of biological chemistry 2004;279(51):53136-44.
2003: Cristina Purcarea; Anupama Ahuja; Tun Lu; Ladislau Christopher Kovari; Hedeel I Guy; David R Evans
Aquifex aeolicus aspartate transcarbamoylase, an enzyme specialized for the efficient utilization of unstable carbamoyl phosphate at elevated temperature.
The Journal of biological chemistry 2003;278(52):52924-34.
2002: Cristina Purcarea; Philip D Martin; John F Vickrey; Hedeel I Guy; Brian F P Edwards; David R Evans
Cloning, expression and preliminary X-ray analysis of the dihydroorotase from the hyperthermophilic eubacterium Aquifex aeolicus.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 1):154-6.
2001: Anupama Ahuja; Cristina Purcarea; Hedeel I Guy; David R Evans
A novel carbamoyl-phosphate synthetase from Aquifex aeolicus.
The Journal of biological chemistry 2001;276(49):45694-703.
2001: Cristina Purcarea; Guy Hervé; Raymond Cunin; David R Evans
Cloning, expression, and structure analysis of carbamate kinase-like carbamoyl phosphate synthetase from Pyrococcus abyssi.
Extremophiles : life under extreme conditions 2001;5(4):229-39.
2001: Cristina Purcarea
Aspartate transcarbamoylase from Pyrococcus abyssi.
Methods in enzymology 2001;331():248-70.
1999: Bernard Labedan; A Boyen; M Baetens; Daniel Charlier; P Chen; Raymond Cunin; V Durbeco; Nicolas Glansdorff; G Herve; Christianne Legrain; Z Liang; Cristina Purcarea; Martine Roovers; R Sanchez; T L Toong; Mark Van de Casteele; F Van Vliet; Y Xu; Y F Zhang
The evolutionary history of carbamoyltransferases: A complex set of paralogous genes was already present in the last universal common ancestor.
Journal of molecular evolution 1999;49(4):461-73.
1999: L Watrin; S Lucas; Cristina Purcarea; Christianne Legrain; Daniel Prieur
Isolation and characterization of pyrimidine auxotrophs, and molecular cloning of the pyrE gene from the hyperthermophilic archaeon Pyrococcus abyssi.
Molecular & general genetics : MGG 1999;262(2):378-81.
1999: Cristina Purcarea; David R Evans; Guy Hervé
Channeling of carbamoyl phosphate to the pyrimidine and arginine biosynthetic pathways in the deep sea hyperthermophilic archaeon Pyrococcus abyssi.
The Journal of biological chemistry 1999;274(10):6122-9.
1997: Cristina Purcarea; Guy Hervé; M M Ladjimi; Raymond Cunin
Aspartate transcarbamylase from the deep-sea hyperthermophilic archaeon Pyrococcus abyssi: genetic organization, structure, and expression in Escherichia coli.
Journal of bacteriology 1997;179(13):4143-57.
1996: Cristina Purcarea; V Simon; Daniel Prieur; Guy Hervé
Purification and characterization of carbamoyl-phosphate synthetase from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi.
European journal of biochemistry / FEBS 1996;236(1):189-99.