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Vinod Bhakuni

Research Profile

Disorders

Protein Denaturation

Physiology

Protein Folding

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Fluorescence Spectrometry

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Amino Acid Sequences

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Publications

TOP 3
Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Novel protein-protein interactions between Entamoeba histolyticad-phosphoglycerate dehydrogenase and phosphoserine aminotransferase.
Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Role of conserved active site tryptophan-101 in functional activity and stability of phosphoserine aminotransferase from an enteric human parasite.
Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Glu-108 is essential for subunit assembly and dimer stability of D-phosphoglycerate dehydrogenase from Entamoeba histolytica.

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All Publications

2012: Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Novel protein-protein interactions between Entamoeba histolyticad-phosphoglycerate dehydrogenase and phosphoserine aminotransferase.
Biochimie 2012;94(8):1676-86.
2012: Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Role of conserved active site tryptophan-101 in functional activity and stability of phosphoserine aminotransferase from an enteric human parasite.
Amino acids 2012;43(1):483-91.
2012: Vibhor Mishra; Tomoyoshi Nozaki; Kam Y J Zhang; Vahab Ali; Ashutosh Kumar; Vinod Bhakuni
Glu-108 is essential for subunit assembly and dimer stability of D-phosphoglycerate dehydrogenase from Entamoeba histolytica.
Molecular and biochemical parasitology 2012;181(2):117-24.
2011: Ranjeet Kumar; Vinod Bhakuni
Comparative analysis of malate synthase G from Mycobacterium tuberculosis and E. coli: role of ionic interaction in modulation of structural and functional properties.
International journal of biological macromolecules 2011;49(5):917-22.
2011: Vibhor Mishra; Vahab Ali; Tomoyoshi Nozaki; Vinod Bhakuni
Biophysical characterization of Entamoeba histolytica phosphoserine aminotransferase (EhPSAT): role of cofactor and domains in stability and subunit assembly.
European biophysics journal : EBJ 2011;40(5):599-610.
2010: Ranjeet Kumar; Vinod Bhakuni
A functionally active dimer of mycobacterium tuberculosis malate synthase G.
European biophysics journal : EBJ 2010;39(11):1557-62.
2010: Anant Narayan Bhatt; Vinod Bhakuni; Ashutosh Kumar; M Yahiya Khan; Mohammad Imran Siddiqi
Alkaline pH-dependent differential unfolding characteristics of mesophilic and thermophilic homologs of dimeric serine hydroxymethyltransferase.
Biochimica et biophysica acta 2010;1804(6):1294-300.
2010: Timir Tripathi; Anne Röseler; Stefan Rahlfs; Katja Becker; Vinod Bhakuni
Conformational stability and energetics of Plasmodium falciparum glutaredoxin.
Biochimie 2010;92(3):284-91.
2010: Vibhor Mishra; Vahab Ali; Tomoyoshi Nozaki; Vinod Bhakuni
Entamoeba histolytica Phosphoserine aminotransferase (EhPSAT): insights into the structure-function relationship.
BMC research notes 2010;3():52.
2009: Timir Tripathi; Byoung-Kuk Na; Woon-Mok Sohn; Katja Becker; Vinod Bhakuni
Structural, functional and unfolding characteristics of glutathione S-transferase of Plasmodium vivax.
Archives of biochemistry and biophysics 2009;487(2):115-22.
2009: Parul Mishra; R Prem Kumar; Abdul S Ethayathulla; Nagendra Singh; Sujata Sharma; Markus Perbandt; Christian Betzel; Punit Kaur; Alagiri Srinivasan; Vinod Bhakuni; Tej P Singh
Polysaccharide binding sites in hyaluronate lyase--crystal structures of native phage-encoded hyaluronate lyase and its complexes with ascorbic acid and lactose.
The FEBS journal 2009;276(12):3392-402.
2009: Kulwant Singh; Vinod Bhakuni
Guanidine hydrochloride- and urea-induced unfolding of Toxoplasma gondii ferredoxin-NADP+ reductase: stabilization of a functionally inactive holo-intermediate.
Journal of biochemistry 2009;145(6):721-31.
2009: Parul Mishra; Vinod Bhakuni
Self-assembly of bacteriophage-associated hyaluronate lyase (HYLP2) into an enzymatically active fibrillar film.
The Journal of biological chemistry 2009;284(8):5240-9.
2009: Gitanjali Yadav; Ramachandra L A Prasad; Babal Kant Jha; VIVEK RAI; Vinod Bhakuni; Kasturi Datta
Evidence for inhibitory interaction of hyaluronan-binding protein 1 (HABP1/p32/gC1qR) with Streptococcus pneumoniae hyaluronidase.
The Journal of biological chemistry 2009;284(6):3897-905.
2008: Anant Narayan Bhatt; Vinod Bhakuni
Characterization of pyridoxal 5'- phosphate-binding domain and folding intermediate of Bacillus subtilis serine hydroxymethyltransferase: an autonomous folding domain.
Journal of biochemistry 2008;144(3):295-303.
2008: Ranjeet Kumar; Vinod Bhakuni
Mycobacterium tuberculosis isocitrate lyase (MtbIcl): role of divalent cations in modulation of functional and structural properties.
Proteins 2008;72(3):892-900.
2008: Timir Tripathi; Stefan Rahlfs; Katja Becker; Vinod Bhakuni
Structural and stability characteristics of a monothiol glutaredoxin: glutaredoxin-like protein 1 from Plasmodium falciparum.
Biochimica et biophysica acta 2008;1784(6):946-52.
2008: Kulwant Singh; Vinod Bhakuni
Toxoplasma gondii ferredoxin-NADP+ reductase: Role of ionic interactions in stabilization of native conformation and structural cooperativity.
Proteins 2008;71(4):1879-88.
2007: Sarita Sharma; Vinod Bhakuni
Cloning and structural analysis of Mycobacterium leprae serine hydroxymethyltransferase.
Protein expression and purification 2007;55(1):189-97.
2007: Md Sohail Akhtar; Vinod Bhakuni
Role of ionic interactions and linker in the domain interaction and modulation of functional activity of hyaluronate lyases.
Biochemical and biophysical research communications 2007;353(2):286-92.
2007: Timir Tripathi; Stefan Rahlfs; Katja Becker; Vinod Bhakuni
Glutathione mediated regulation of oligomeric structure and functional activity of Plasmodium falciparum glutathione S-transferase.
BMC structural biology 2007;7():67.
2007: Kulwant Singh; Vinod Bhakuni
Cation induced differential effect on structural and functional properties of Mycobacterium tuberculosis alpha-isopropylmalate synthase.
BMC structural biology 2007;7():39.
2006: Md Sohail Akhtar; Manju Yasoda Krishnan; Vinod Bhakuni
Insights into the mechanism of action of hyaluronate lyase: role of C-terminal domain and Ca2+ in the functional regulation of enzyme.
The Journal of biological chemistry 2006;281(38):28336-44.
2006: Parul Mishra; Md Sohail Akhtar; Vinod Bhakuni
Unusual structural features of the bacteriophage-associated hyaluronate lyase (hylp2).
The Journal of biological chemistry 2006;281(11):7143-50.
2005: Nidhi Shukla; Anant Narayan Bhatt; Alessandro Aliverti; Giuliana Zanetti; Vinod Bhakuni
Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase: stabilization of an apo-protein by GdmCl.
The FEBS journal 2005;272(9):2216-24.
2005: Anant Narayan Bhatt; Nidhi Shukla; Alessandro Aliverti; Giuliana Zanetti; Vinod Bhakuni
Modulation of cooperativity in Mycobacterium tuberculosis NADPH-ferredoxin reductase: cation-and pH-induced alterations in native conformation and destabilization of the NADP+-binding domain.
Protein science : a publication of the Protein Society 2005;14(4):980-92.
2004: Anant Narayan Bhatt; Mohammad Khan; Vinod Bhakuni
The C-terminal domain of dimeric serine hydroxymethyltransferase plays a key role in stabilization of the quaternary structure and cooperative unfolding of protein: domain swapping studies with enzymes having high sequence identity.
Protein science : a publication of the Protein Society 2004;13(8):2184-95.
2004: Aradhana Awasthi Tiwari; Guru P Dutta; Vinod Bhakuni; Renu Tripathi
Resistance reversal action of ketoconazole against mefloquine resistance of Plasmodium yoelii nigeriensis.
Experimental parasitology 2004;107(3-4):115-9.
2003: Sarita Chaturvedi; Vinod Bhakuni
Unusual structural, functional, and stability properties of serine hydroxymethyltransferase from Mycobacterium tuberculosis.
The Journal of biological chemistry 2003;278(42):40793-805.
2003: Md Sohail Akhtar; Vinod Bhakuni
Streptococcus pneumoniae hyaluronate lyase contains two non-cooperative independent folding/unfolding structural domains: characterization of functional domain and inhibitors of enzyme.
The Journal of biological chemistry 2003;278(28):25509-16.
2003: Md Sohail Akhtar; Vinod Bhakuni
Alkaline treatment has contrasting effects on the structure of deglycosylated and glycosylated forms of glucose oxidase.
Archives of biochemistry and biophysics 2003;413(2):221-8.
2003: Sandeep K Misra; Vinod Bhakuni
Unique holoenzyme dimers of the tetrameric enzyme Escherichia coli methylenetetrahydrofolate reductase: characterization of structural features associated with modulation of the enzyme's function.
Biochemistry 2003;42(13):3921-8.
2002: Anant Narayan Bhatt; Koodathingal Prakash; Hosahalli S Subramanya; Vinod Bhakuni
Different unfolding pathways for mesophilic and thermophilic homologues of serine hydroxymethyltransferase.
Biochemistry 2002;41(40):12115-23.
2002: Md Sohail Akhtar; Atta Ahmad; Vinod Bhakuni
Divalent cation induced changes in structural properties of the dimeric enzyme glucose oxidase: dual effect of dimer stabilization and dissociation with loss of cooperative interactions in enzyme monomer.
Biochemistry 2002;41(22):7142-9.
2002: Md Sohail Akhtar; Atta Ahmad; Vinod Bhakuni
Guanidinium chloride- and urea-induced unfolding of the dimeric enzyme glucose oxidase.
Biochemistry 2002;41(11):3819-27.
2002: Koodathingal Prakash; Shashi Prajapati; Atta Ahmad; S K Jain; Vinod Bhakuni
Unique oligomeric intermediates of bovine liver catalase.
Protein science : a publication of the Protein Society 2002;11(1):46-57.
2001: Atta Ahmad; Md Sohail Akhtar; Vinod Bhakuni
Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme.
Biochemistry 2001;40(7):1945-55.
2000: Atta Ahmad; Kunnath P Madhusudanan; Vinod Bhakuni
Trichloroacetic acid and trifluoroacetic acid-induced unfolding of cytochrome c: stabilization of a native-like folded intermediate(1).
Biochimica et biophysica acta 2000;1480(1-2):201-10.
1999: Vahab Ali; Koodathingal Prakash; S Kulkarni; Atta Ahmad; K P Madhusudan; Vinod Bhakuni
8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.
Biochemistry 1999;38(41):13635-42.
1999: Vinod Bhakuni; S Kulkarni; Vahab Ali; U K Singh; H B Levy; RK Maheshwari
Immunochemotherapy for Leishmania donovani infection in golden hamsters: combinatorial action of poly ICLC plus L-arginine and sodium stibogluconate (Stibanate).
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 1999;19(10):1103-6.
1999: N Gupta; Neena Goyal; U K Singha; Vinod Bhakuni; R Roy; AK Rastogi
Characterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy.
Acta tropica 1999;73(2):121-33.
1998: Vinod Bhakuni
Alcohol-induced molten globule intermediates of proteins: are they real folding intermediates or off pathway products?
Archives of biochemistry and biophysics 1998;357(2):274-84.
1998: S Prajapati; Vinod Bhakuni; K R Babu; S K Jain
Alkaline unfolding and salt-induced folding of bovine liver catalase at high pH.
European journal of biochemistry / FEBS 1998;255(1):178-84.
1997: Aradhana Awasthi Tiwari; S Mehrotra; Vinod Bhakuni; Guru P Dutta; H B Levy; RK Maheshwari
Poly ICLC enhances the antimalarial activity of chloroquine against multidrug-resistant Plasmodium yoelii nigeriensis in mice.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 1997;17(7):419-23.
1997: K R Babu; Vinod Bhakuni
Ionic-strength-dependent transition of hen egg-white lysozyme at low pH to a compact state and its aggregation on thermal denaturation.
European journal of biochemistry / FEBS 1997;245(3):781-9.
1996: U K Singha; Vinod Bhakuni; Vahab Ali; R Roy
Leishmania donovani: metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy.
Molecular and cellular biochemistry 1996;162(1):17-22.
1996: Vinod Bhakuni; U K Singha; Guru P Dutta; H B Levy; RK Maheshwari
Killing of Leishmania donovani amastigotes by poly ICLC in hamsters.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 1996;16(4):321-5.
1994: Vinod Bhakuni; R Roy
Interaction of trichloroethylene with phosphatidylcholine and its localization in the phosphatidylcholine vesicles: a 1H-NMR study.
Biochemical pharmacology 1994;47(8):1461-4.
1990: A Kumar; S R Gudi; S M Gokhale; Vinod Bhakuni; Chhitar M Gupta
Heat-induced alterations in monkey erythrocyte membrane phospholipid organization and skeletal protein structure and interactions.
Biochimica et biophysica acta 1990;1030(2):269-78.
1990: S R Gudi; A Kumar; Vinod Bhakuni; S M Gokhale; Chhitar M Gupta
Membrane skeleton-bilayer interaction is not the major determinant of membrane phospholipid asymmetry in human erythrocytes.
Biochimica et biophysica acta 1990;1023(1):63-72.
1989: Vinod Bhakuni; Chhitar M Gupta
Carbamyl phosphatidylcholine--cholesterol interactions in unilamellar vesicles.
Biochimica et biophysica acta 1989;982(2):216-22.
1986: Vinod Bhakuni; C M Gupta
Interactions of tuftsin with bovine serum albumin.
FEBS letters 1986;205(2):347-50.