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Barbara Rehermann

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Eui-Cheol Shin; Charles M Rice; Laura Caggiari; Valli De Re; Stephen M Feinstone; Marian Major; Michelina Nascimbeni; Su-Hyung Park; Barbara Rehermann
The frequency of CD127(+) hepatitis C virus (HCV)-specific T cells but not the expression of exhaustion markers predicts the outcome of acute HCV infection.
Ludmila Prokunina-Olsson; Barbara Rehermann; Faruk Sheikh; Wei Tang; McAnthony Tarway; David L Thomas; Heiyoung Park; Ruth M Pfeiffer; Patricia Porter-Gill; Jacquie Astemborski; Herbert L Bonkovsky; Nathan Brand; Sabrina Chen; Harold Dickensheets; Raymond P Donnelly; Brian R Edlin; Dianna Hergott; Charles D Howell; Indu kohaar; Luyang Liu; Timothy R Morgan; Brian Muchmore; Adam Mumy; Thomas R O'Brien
A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus.
Heiyoung Park; Ludmila Prokunina-Olsson; Elisavet Serti; Stefania Capone; Onyinyechi Eke; Antonella Folgori; Brian Muchmore; Barbara Rehermann
IL-29 is the dominant type III interferon produced by hepatocytes during acute hepatitis C virus infection.

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2013: Eui-Cheol Shin; Charles M Rice; Laura Caggiari; Valli De Re; Stephen M Feinstone; Marian Major; Michelina Nascimbeni; Su-Hyung Park; Barbara Rehermann
The frequency of CD127(+) hepatitis C virus (HCV)-specific T cells but not the expression of exhaustion markers predicts the outcome of acute HCV infection.
Journal of virology 2013;87(8):4772-7.
2013: Ludmila Prokunina-Olsson; Barbara Rehermann; Faruk Sheikh; Wei Tang; McAnthony Tarway; David L Thomas; Heiyoung Park; Ruth M Pfeiffer; Patricia Porter-Gill; Jacquie Astemborski; Herbert L Bonkovsky; Nathan Brand; Sabrina Chen; Harold Dickensheets; Raymond P Donnelly; Brian R Edlin; Dianna Hergott; Charles D Howell; Indu kohaar; Luyang Liu; Timothy R Morgan; Brian Muchmore; Adam Mumy; Thomas R O'Brien
A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus.
Nature genetics 2013;45(2):164-71.
2012: Heiyoung Park; Ludmila Prokunina-Olsson; Elisavet Serti; Stefania Capone; Onyinyechi Eke; Antonella Folgori; Brian Muchmore; Barbara Rehermann
IL-29 is the dominant type III interferon produced by hepatocytes during acute hepatitis C virus infection.
Hepatology (Baltimore, Md.) 2012;56(6):2060-70.
2012: Lauren E Holz; Susan Moir; Sukanya Raghuraman; Michael C Sneller; Joo Chun Yoon; Barbara Rehermann
B cell homeostasis in chronic hepatitis C virus-related mixed cryoglobulinemia is maintained through naïve B cell apoptosis.
Hepatology (Baltimore, Md.) 2012;56(5):1602-10.
2012: Su-Hyung Park; Eui-Cheol Shin; Laura Caggiari; Stefania Capone; Valli De Re; Antonella Folgori; Alfredo Nicosia; Barbara Rehermann
Successful vaccination induces multifunctional memory T-cell precursors associated with early control of hepatitis C virus.
Gastroenterology 2012;143(4):1048-60.e4.
2012: Addisalem T Makuria; Sukanya Raghuraman; Barbara Rehermann; Robert D Allison; Peter D Burbelo; Cathy C Cantilena; JOAN WHITEHOUSE-GIBBLE; Harvey J Alter
The clinical relevance of persistent recombinant immunoblot assay-indeterminate reactions: insights into the natural history of hepatitis C virus infection and implications for donor counseling.
Transfusion 2012;52(9):1940-8.
2012: Michael P Busch; Eric L Delwart; Belinda L Herring; Dale Hirschkorn; Steven H Kleinman; Krishna K Murthy; Vito Racanelli; Barbara Rehermann; Joo Chun Yoon; Harvey J Alter
Infectivity in chimpanzees (Pan troglodytes) of plasma collected before HCV RNA detectability by FDA-licensed assays: implications for transfusion safety and HCV infection outcomes.
Blood 2012;119(26):6326-34.
2012: Sukanya Raghuraman; Brian R Edlin; William O Osburn; Heiyoung Park; Emily Winkelstein; Barbara Rehermann
Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
The Journal of infectious diseases 2012;205(5):763-71.
2011: Golo Ahlenstiel; Birgit Edlich; Jordan J Feld; Leah J Hogdal; Lauren E Holz; TSANYANG LIANG; Mazen Noureddin; Yaron Rotman; Rachel H Titerence; Barbara Rehermann
Early changes in natural killer cell function indicate virologic response to interferon therapy for hepatitis C.
Gastroenterology 2011;141(4):1231-9, 1239.e1-2.
2011: Marija Zeremski; Leslie H Tobler; Emily Winkelstein; Queenie Brown; Michael P Busch; Don C Des Jarlais; Brian R Edlin; Giles Hooker; Barbara Rehermann; Marla A Shu; Andrew H Talal
Induction of CXCR3- and CCR5-associated chemokines during acute hepatitis C virus infection.
Journal of hepatology 2011;55(3):545-53.
2011: Eui-Cheol Shin; Naga S Veerapu; Charles M Rice; Mary Demino; Stephen M Feinstone; Theo Heller; Marian E Major; Kathleen Mihalik; Michelina Nascimbeni; Su-Hyung Park; Barbara Rehermann
Delayed induction, not impaired recruitment, of specific CD8⁺ T cells causes the late onset of acute hepatitis C.
Gastroenterology 2011;141(2):686-95, 695.e1.
2011: Naga Suresh Veerapu; Sukanya Raghuraman; TSANYANG LIANG; Theo Heller; Barbara Rehermann
Sporadic reappearance of minute amounts of hepatitis C virus RNA after successful therapy stimulates cellular immune responses.
Gastroenterology 2011;140(2):676-685.e1.
2010: Birgit Edlich; Leah J Hogdal; Barbara Rehermann; Sven-Erik Behrens
Dendritic cells transfected with Her2 antigen-encoding RNA replicons cross-prime CD8 T cells and protect mice against tumor challenge.
Vaccine 2010;28(49):7764-73.
2010: Laura Caggiari; M De Zorzi; Barbara Rehermann; Valli De Re
Identification of a new Patr-B*01 variant, Patr-B*0102, by sequence-based typing in a chimpanzee (Pan troglodytes).
Tissue antigens 2010;75(2):186-8.
2010: Golo Ahlenstiel; Rachel H Titerence; Christopher Koh; Birgit Edlich; Jordan J Feld; Yaron Rotman; Marc G Ghany; Jay H Hoofnagle; TSANYANG LIANG; Theo Heller; Barbara Rehermann
Natural killer cells are polarized toward cytotoxicity in chronic hepatitis C in an interferon-alfa-dependent manner.
Gastroenterology 2010;138(1):325-35.e1-2.
2009: Barbara Rehermann
Hepatitis C virus versus innate and adaptive immune responses: a tale of coevolution and coexistence.
The Journal of clinical investigation 2009;119(7):1745-54.
2009: Brian R Edlin; Marla A Shu; Emily Winkelstein; Don C Des Jarlais; Michael P Busch; Barbara Rehermann; Thomas R O'Brien; Andrew H Talal; Leslie H Tobler; Marija Zeremski; Ann B Beeder
More rare birds, and the occasional swan.
Gastroenterology 2009;136(7):2412-4.
2009: Theo Heller; Jay Hoofnagle; TSANYANG LIANG; Barbara Rehermann
Acute hepatitis C.
Gastroenterology 2009;136(7):2411.
2009: Joo Chun Yoon; Masaaki Shiina; Golo Ahlenstiel; Barbara Rehermann
Natural killer cell function is intact after direct exposure to infectious hepatitis C virions.
Hepatology (Baltimore, Md.) 2009;49(1):12-21.
2009: Robert E Lanford; Matthew J Evans; Volker Lohmann; Brett Lindenbach; Michael Gale; Barbara Rehermann; Kyong-Mi Chang; Stanley M Lemon
The accelerating pace of HCV research: a summary of the 15th International Symposium on Hepatitis C Virus And Related Viruses.
Gastroenterology 2009;136(1):9-16.
2009: Masaaki Shiina; Barbara Rehermann
Analysis of HCV-specific T cells by flow cytometry.
Methods in molecular biology (Clifton, N.J.) 2009;510():415-26.
2009: Joo Chun Yoon; Barbara Rehermann
Determination of HCV-specific T-cell activity.
Methods in molecular biology (Clifton, N.J.) 2009;510():403-13.
2008: Eui-Cheol Shin; Stefania Capone; Riccardo Cortese; Stefano Colloca; Alfredo Nicosia; Antonella Folgori; Barbara Rehermann
The kinetics of hepatitis C virus-specific CD8 T-cell responses in the blood mirror those in the liver in acute hepatitis C virus infection.
Journal of virology 2008;82(19):9782-8.
2008: Eishiro Mizukoshi; Christoph Eisenbach; Brian R Edlin; Kimberly P Newton; Sukanya Raghuraman; Christina Weiler-Normann; Leslie H Tobler; Michael P Busch; Mary Carrington; Jane A McKeating; Thomas R O'Brien; Barbara Rehermann
Hepatitis C virus (HCV)-specific immune responses of long-term injection drug users frequently exposed to HCV.
The Journal of infectious diseases 2008;198(2):203-12.
2008: Laura Caggiari; M De Zorzi; Golo Ahlenstiel; Barbara Rehermann; Valli De Re
Identification of new major histocompatibility complex-A, -B, -C alleles in chimpanzees (Pan troglodytes).
Tissue antigens 2008;72(1):79-83.
2008: M De Zorzi; Laura Caggiari; Golo Ahlenstiel; Barbara Rehermann; Valli De Re
Description of two new major histocompatibility complex (MHC) class II DRB1 [Pan troglodytes (Patr)-DRB1] alleles.
Tissue antigens 2008;71(5):490-2.
2008: Golo Ahlenstiel; Maureen P Martin; Xiaojiang Gao; Mary Carrington; Barbara Rehermann
Distinct KIR/HLA compound genotypes affect the kinetics of human antiviral natural killer cell responses.
The Journal of clinical investigation 2008;118(3):1017-26.
2008: Masaaki Shiina; Barbara Rehermann
Cell culture-produced hepatitis C virus impairs plasmacytoid dendritic cell function.
Hepatology (Baltimore, Md.) 2008;47(2):385-95.
2007: Eui-Cheol Shin; Ulrike Seifert; Sabrina Urban; Kim-Thuy Truong; Stephen M Feinstone; Charles M Rice; Peter-M Kloetzel; Barbara Rehermann
Proteasome activator and antigen-processing aminopeptidases are regulated by virus-induced type I interferon in the hepatitis C virus-infected liver.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2007;27(12):985-90.
2007: Flavien Bernardin; Susan L Stramer; Barbara Rehermann; Kimberly Page-Shafer; Stewart Cooper; david bangsberg; Judith Hahn; Leslie Tobler; Michael Busch; Eric L Delwart
High levels of subgenomic HCV plasma RNA in immunosilent infections.
Virology 2007;365(2):446-56.
2007: Laura Caggiari; Barbara Rehermann; A Folgori; Valli De Re
Identification of four novel MHC-C alleles in chimpanzees.
Tissue antigens 2007;70(1):78-9.
2007: Barbara Rehermann
Chronic infections with hepatotropic viruses: mechanisms of impairment of cellular immune responses.
Seminars in liver disease 2007;27(2):152-60.
2007: Barbara Rehermann; Nikolai Naoumov
Immunological techniques in viral hepatitis.
Journal of hepatology 2007;46(3):508-20.
2007: Thomas von Hahn; Joo Chun Yoon; Harvey Alter; Charles M Rice; Barbara Rehermann; Peter Balfe; Jane A McKeating
Hepatitis C virus continuously escapes from neutralizing antibody and T-cell responses during chronic infection in vivo.
Gastroenterology 2007;132(2):667-78.
2006: Eui-Cheol Shin; Ulrike Seifert; Takanobu Kato; Charles M Rice; Stephen M Feinstone; Peter-M Kloetzel; Barbara Rehermann
Virus-induced type I IFN stimulates generation of immunoproteasomes at the site of infection.
The Journal of clinical investigation 2006;116(11):3006-14.
2006: Masaaki Shiina; Barbara Rehermann
Hepatitis C vaccines: Inducing and challenging memory T cells.
Hepatology (Baltimore, Md.) 2006;43(6):1395-8.
2006: Tobias Manigold; Eui-Cheol Shin; Eishiro Mizukoshi; Kathleen Mihalik; Krishna K Murthy; Charles M Rice; Ciriaco Piccirillo; Barbara Rehermann
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Blood 2006;107(11):4424-32.
2006: Eui-Cheol Shin; Barbara Rehermann
Taking the brake off T cells in chronic viral infection.
Nature medicine 2006;12(3):276-7.
2006: Vito Racanelli; Barbara Rehermann
The liver as an immunological organ.
Hepatology (Baltimore, Md.) 2006;43(2 Suppl 1):S54-62.
2006: Laura Caggiari; Maria Paola Simula; Alessandra Marzotto; Masaaki Shiina; Barbara Rehermann; Valli De Re
Identification of novel chimpanzee MHC class I and II alleles using an improved sequence-based typing strategy.
Human immunology 2006;67(1-2):63-72.
2005: Eui-Cheol Shin; Ulrike Protzer; Andreas Untergasser; Stephen M Feinstone; Charles M Rice; Dana Hasselschwert; Barbara Rehermann
Liver-directed gamma interferon gene delivery in chronic hepatitis C.
Journal of virology 2005;79(21):13412-20.
2005: Pierre Vandepapelière; Barbara Rehermann; Marguerite Koutsoukos; Philippe Moris; Nathalie Garçon; Martine A Wettendorff; Geert Leroux-Roels
Potent enhancement of cellular and humoral immune responses against recombinant hepatitis B antigens using AS02A adjuvant in healthy adults.
Vaccine 2005;23(20):2591-601.
2005: Barbara Rehermann; Eui-Cheol Shin
Private aspects of heterologous immunity.
The Journal of experimental medicine 2005;201(5):667-70.
2005: Barbara Rehermann; Michelina Nascimbeni
Immunology of hepatitis B virus and hepatitis C virus infection.
Nature reviews. Immunology 2005;5(3):215-29.
2005: Golo Ahlenstiel; Barbara Rehermann
Hepatitis C virus and the threshold of natural killer cell inhibition.
Hepatology (Baltimore, Md.) 2005;41(3):675-7.
2005: Theo Heller; Barbara Rehermann
Acute hepatitis C: a multifaceted disease.
Seminars in liver disease 2005;25(1):7-17.
2004: Eishiro Mizukoshi; John Sidney; Brian Livingston; Marc G Ghany; Jay H Hoofnagle; Alessandro Sette; Barbara Rehermann
Cellular immune responses to the hepatitis B virus polymerase.
Journal of immunology (Baltimore, Md. : 1950) 2004;173(9):5863-71.
2004: Michelina Nascimbeni; Eui-Cheol Shin; Luis Chiriboga; David E Kleiner; Barbara Rehermann
Peripheral CD4(+)CD8(+) T cells are differentiated effector memory cells with antiviral functions.
Blood 2004;104(2):478-86.
2004: Ulrike Seifert; Heike Liermann; Vito Racanelli; Anne Halenius; Manfred Wiese; Heiner Wedemeyer; Thomas Ruppert; Kay Rispeter; Peter Henklein; Alice J A M Sijts; Hartmut Hengel; Peter-M Kloetzel; Barbara Rehermann
Hepatitis C virus mutation affects proteasomal epitope processing.
The Journal of clinical investigation 2004;114(2):250-9.
2004: Fareed Rahman; Theo Heller; Yuji Sobao; Eishiro Mizukoshi; Michelina Nascimbeni; Harvey Alter; Steven Herrine; Jay H Hoofnagle; TSANYANG LIANG; Barbara Rehermann
Effects of antiviral therapy on the cellular immune response in acute hepatitis C.
Hepatology (Baltimore, Md.) 2004;40(1):87-97.
2004: Sook-Hyang Jeong; Ming Qiao; Michelina Nascimbeni; Zongyi Hu; Barbara Rehermann; Krishna K Murthy; TSANYANG LIANG
Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.
Journal of virology 2004;78(13):6995-7003.
2004: Fareed Rahman; Barbara Rehermann
Ethnicity and hepatitis C virus infection.
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 2004;2(6):456-8.
2004: Michelina Nascimbeni; Barbara Rehermann
Determination of hepatitis B virus-specific CD8+ T-cell activity in the liver.
Methods in molecular medicine 2004;96():65-83.
2004: Vito Racanelli; Sven Erik Behrens; Julio Aliberti; Barbara Rehermann
Dendritic cells transfected with cytopathic self-replicating RNA induce crosspriming of CD8+ T cells and antiviral immunity.
Immunity 2004;20(1):47-58.
2003: Vito Racanelli; Barbara Rehermann
Hepatitis C virus infection: when silence is deception.
Trends in immunology 2003;24(8):456-64.
2003: Michelina Nascimbeni; Barbara Rehermann
Chronic HCV infection and the clonality of intrahepatic T cells.
Journal of hepatology 2003;38(5):677-80.
2003: Michelina Nascimbeni; Eishiro Mizukoshi; Markus Bosmann; Marian E Major; Kathleen Mihalik; Charles M Rice; Stephen M Feinstone; Barbara Rehermann
Kinetics of CD4+ and CD8+ memory T-cell responses during hepatitis C virus rechallenge of previously recovered chimpanzees.
Journal of virology 2003;77(8):4781-93.
2003: Tobias Manigold; Barbara Rehermann
Chronic hepatitis B and hepatocarcinogenesis: does prevention of "collateral damage" bring the cure?
Hepatology (Baltimore, Md.) 2003;37(3):707-10.
2003: Barbara Rehermann
Immune responses in hepatitis B virus infection.
Seminars in liver disease 2003;23(1):21-38.
2003: Michael Thomson; Michelina Nascimbeni; Michael B Havert; Marian E Major; Sophia Gonzales; Harvey Alter; Stephen M Feinstone; Krishna K Murthy; Barbara Rehermann; TSANYANG LIANG
The clearance of hepatitis C virus infection in chimpanzees may not necessarily correlate with the appearance of acquired immunity.
Journal of virology 2003;77(2):862-70.
2002: Heiner Wedemeyer; Xiaosong He; Michelina Nascimbeni; Anthony R Davis; Harry B Greenberg; Jay H Hoofnagle; TSANYANG LIANG; Harvey Alter; Barbara Rehermann
Impaired effector function of hepatitis C virus-specific CD8+ T cells in chronic hepatitis C virus infection.
Journal of immunology (Baltimore, Md. : 1950) 2002;169(6):3447-58.
2002: Marian E Major; Kathleen Mihalik; Montserrat Puig; Barbara Rehermann; Michelina Nascimbeni; Charles M Rice; Stephen M Feinstone
Previously infected and recovered chimpanzees exhibit rapid responses that control hepatitis C virus replication upon rechallenge.
Journal of virology 2002;76(13):6586-95.
2002: Eishiro Mizukoshi; Michelina Nascimbeni; Joshua B Blaustein; Kathleen Mihalik; Charles M Rice; TSANYANG LIANG; Stephen M Feinstone; Barbara Rehermann
Molecular and immunological significance of chimpanzee major histocompatibility complex haplotypes for hepatitis C virus immune response and vaccination studies.
Journal of virology 2002;76(12):6093-103.
2001: Eishiro Mizukoshi; Barbara Rehermann
Immune responses and immunity in hepatitis C virus infection.
Journal of gastroenterology 2001;36(12):799-808.
2001: J Satoi; Kazumoto Murata; Martin Lechmann; E Manickan; Zhensheng Zhang; Heiner Wedemeyer; Barbara Rehermann; TSANYANG LIANG
Genetic immunization of wild-type and hepatitis C virus transgenic mice reveals a hierarchy of cellular immune response and tolerance induction against hepatitis C virus structural proteins.
Journal of virology 2001;75(24):12121-7.
2001: Heiner Wedemeyer; Eishiro Mizukoshi; Anthony R Davis; Jack R Bennink; Barbara Rehermann
Cross-reactivity between hepatitis C virus and Influenza A virus determinant-specific cytotoxic T cells.
Journal of virology 2001;75(23):11392-400.
2001: Michael Thomson; Michelina Nascimbeni; S Gonzales; Krishna K Murthy; Barbara Rehermann; TSANYANG LIANG
Emergence of a distinct pattern of viral mutations in chimpanzees infected with a homogeneous inoculum of hepatitis C virus.
Gastroenterology 2001;121(5):1226-33.
2001: Heiner Wedemeyer; Sara Gagneten; A Davis; R Bartenschlager; Stephen M Feinstone; Barbara Rehermann
Oral immunization with HCV-NS3-transformed Salmonella: induction of HCV-specific CTL in a transgenic mouse model.
Gastroenterology 2001;121(5):1158-66.
2000: A Takaki; M Wiese; Geert Maertens; Erik Depla; Ulrike Seifert; A Liebetrau; J L Miller; Michael P Manns; Barbara Rehermann
Cellular immune responses persist and humoral responses decrease two decades after recovery from a single-source outbreak of hepatitis C.
Nature medicine 2000;6(5):578-82.
2000: Barbara Rehermann
Intrahepatic T cells in hepatitis B: viral control versus liver cell injury.
The Journal of experimental medicine 2000;191(8):1263-8.
2000: TSANYANG LIANG; Barbara Rehermann; Leonard B Seeff; Jay H Hoofnagle
Pathogenesis, natural history, treatment, and prevention of hepatitis C.
Annals of internal medicine 2000;132(4):296-305.
2000: Alice J A M Sijts; Thomas Ruppert; Barbara Rehermann; Marion Schmidt; Ulrich H Koszinowski; Peter-M Kloetzel
Efficient generation of a hepatitis B virus cytotoxic T lymphocyte epitope requires the structural features of immunoproteasomes.
The Journal of experimental medicine 2000;191(3):503-14.
2000: Barbara Rehermann
Interaction between the hepatitis C virus and the immune system.
Seminars in liver disease 2000;20(2):127-41.
2000: Barbara Rehermann; F V Chisari
Cell mediated immune response to the hepatitis C virus.
Current topics in microbiology and immunology 2000;242():299-325.
1998: K Klugewitz; Barbara Rehermann; Ulrike Seifert; Klaus H W Böker; M Stolte; Peter N Meier; Siegfried Wagner; Michael P Manns
A rare case of bloody diarrhea: thrombosis of the V. mesenterica inferior following laparoscopic cholecystectomy.
Zeitschrift für Gastroenterologie 1998;36(1):35-9.
1997: Barbara Rehermann; K Klugewitz; Ulrike Seifert; Klaus H W Böker; Siegfried Wagner; Michael P Manns
[Rare initial manifestation and differential diagnosis of primary sclerosing cholangitis].
Medizinische Klinik (Munich, Germany : 1983) 1997;92(7):452-6.
1996: Barbara Rehermann; Ulrike Seifert; Hans L Tillmann; G Michel; Klaus H W Böker; Rudolf Pichlmayr; Michael P Manns
Serological pattern of hepatitis C virus recurrence after liver transplantation.
Journal of hepatology 1996;24(1):15-20.
1994: Barbara Rehermann; A Schneider; G Michel; H B Braun; Michael P Manns
GOR-antibodies in patients with chronic liver disease and hepatocellular carcinoma.
Zeitschrift für Gastroenterologie 1994;32(7):396-8.
1994: Barbara Rehermann; K Michitaka; Marilena Durazzo; K Mergener; P Velev; Michael P Manns
Viruses and auto-immune hepatitis.
European journal of clinical investigation 1994;24(1):11-9.
1992: Barbara Rehermann; Christian Trautwein; Klaus H W Böker; Michael P Manns
Interleukin-6 in liver diseases.
Journal of hepatology 1992;15(3):277-80.