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Juha Rouvinen

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Trichoderma

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Amino Acid Sequences

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Publications

TOP 3
Mirko Maksimainen; Sari Paavilainen; Nina Hakulinen; Juha Rouvinen
Structural analysis, enzymatic characterization, and catalytic mechanisms of β-galactosidase from Bacillus circulans sp. alkalophilus.
Johanna M Kallio; Juha Rouvinen
Amphiphilic nanotubes in the crystal structure of a biosurfactant protein hydrophobin HFBII.
Juha P Kallio; Anu Koivula; Kristiina Kruus; Juha Rouvinen; Martina Andberg; Harry Boer; Chiara Gasparetti; Nina Hakulinen
Crystal structure of an ascomycete fungal laccase from Thielavia arenaria--common structural features of asco-laccases.

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All Publications

2012: Mirko Maksimainen; Sari Paavilainen; Nina Hakulinen; Juha Rouvinen
Structural analysis, enzymatic characterization, and catalytic mechanisms of β-galactosidase from Bacillus circulans sp. alkalophilus.
The FEBS journal 2012;279(10):1788-98.
2011: Johanna M Kallio; Juha Rouvinen
Amphiphilic nanotubes in the crystal structure of a biosurfactant protein hydrophobin HFBII.
Chemical communications (Cambridge, England) 2011;47(35):9843-5.
2011: Juha P Kallio; Anu Koivula; Kristiina Kruus; Juha Rouvinen; Martina Andberg; Harry Boer; Chiara Gasparetti; Nina Hakulinen
Crystal structure of an ascomycete fungal laccase from Thielavia arenaria--common structural features of asco-laccases.
The FEBS journal 2011;278(13):2283-95.
2011: Heidi Kaljunen; Chiara Gasparetti; Kristiina Kruus; Juha Rouvinen; Nina Hakulinen
Crystallization and preliminary X-ray analysis of Aspergillus oryzae catechol oxidase.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 6):672-4.
2011: Juha P Kallio; Juha Rouvinen; Kristiina Kruus; Nina Hakulinen
Probing the dioxygen route in Melanocarpus albomyces laccase with pressurized xenon gas.
Biochemistry 2011;50(21):4396-8.
2011: Mirko Maksimainen; Nina Hakulinen; Johanna M Kallio; Tommi Timoharju; Ossi Turunen; Juha Rouvinen
Crystal structures of Trichoderma reesei β-galactosidase reveal conformational changes in the active site.
Journal of structural biology 2011;174(1):156-63.
2011: Merja H Niemi; Kristiina Takkinen; Lotta K Amundsen; Hans Söderlund; Juha Rouvinen; Matti Höyhtyä
The testosterone binding mechanism of an antibody derived from a naïve human scFv library.
Journal of molecular recognition : JMR 2011;24(2):209-19.
2010: Merja H Niemi; Laura Turunen; Timo Pulli; Tarja K Nevanen; Matti Höyhtyä; Hans Söderlund; Juha Rouvinen; Kristiina Takkinen
A structural insight into the molecular recognition of a (-)-Delta9-tetrahydrocannabinol and the development of a sensitive, one-step, homogeneous immunocomplex-based assay for its detection.
Journal of molecular biology 2010;400(4):803-14.
2010: Juha Rouvinen; Janne Jänis; Marja-Leena Laukkanen; Sirpa Jylhä; Merja Niemi; Tero Päivinen; Soili Mäkinen-Kiljunen; Tari Haahtela; Hans Söderlund; Kristiina Takkinen
Transient dimers of allergens.
PloS one 2010;5(2):e9037.
2009: Martina Andberg; Nina Hakulinen; Sanna Auer; Markku Saloheimo; Anu Koivula; Juha Rouvinen; Kristiina Kruus
Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure.
The FEBS journal 2009;276(21):6285-300.
2009: J P Kallio; S Auer; Janne Jänis; M Andberg; Kristiina Kruus; Juha Rouvinen; Anu Koivula; Nina Hakulinen
Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds.
Journal of molecular biology 2009;392(4):895-909.
2009: Juha Pekka Kallio; Janne Jänis; Antti Nyyssölä; Nina Hakulinen; Juha Rouvinen
Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 8):805-8.
2009: Mirko Maksimainen; Tommi Timoharju; Johanna M Kallio; Nina Hakulinen; Ossi Turunen; Juha Rouvinen
Crystallization and preliminary diffraction analysis of a beta-galactosidase from Trichoderma reesei.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 8):767-9.
2009: Johanna M Kallio; Nina Hakulinen; Juha P Kallio; Merja H Niemi; Susanna Kärkkäinen; Juha Rouvinen
The contribution of polystyrene nanospheres towards the crystallization of proteins.
PloS one 2009;4(1):e4198.
2008: Janne Jänis; Salla Pasanen; Juha Rouvinen; Pirjo Vainiotalo
Characterization of the pH-dependent dissociation of a multimeric metalloprotein Streptomyces rubiginosus xylose isomerase by ESI FT-ICR mass spectrometry.
Journal of mass spectrometry : JMS 2008;43(10):1376-80.
2008: Tarja Parkkinen; Anu Koivula; Jari Vehmaanperä; Juha Rouvinen
Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding.
Protein science : a publication of the Protein Society 2008;17(8):1383-94.
2008: Nina Hakulinen; M Andberg; Johanna M Kallio; Anu Koivula; Kristiina Kruus; Juha Rouvinen
A near atomic resolution structure of a Melanocarpus albomyces laccase.
Journal of structural biology 2008;162(1):29-39.
2008: Soili Saarelainen; Marja Rytkönen-Nissinen; Juha Rouvinen; Antti Taivainen; Seppo Auriola; A Kauppinen; Tuure Kinnunen; Tuomas Virtanen
Animal-derived lipocalin allergens exhibit immunoglobulin E cross-reactivity.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2008;38(2):374-81.
2008: Merja Niemi; Janne Jänis; Sirpa Jylhä; Johanna M Kallio; Nina Hakulinen; Marja-Leena Laukkanen; Kristiina Takkinen; Juha Rouvinen
Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine beta-lactoglobulin allergen.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 1):25-8.
2008: Janne Jänis; Juha Rouvinen; Pirjo Vainiotalo; Ossi Turunen; Valery L Shnyrov
Irreversible thermal denaturation of Trichoderma reesei endo-1,4-beta-xylanase II and its three disulfide mutants characterized by differential scanning calorimetry.
International journal of biological macromolecules 2008;42(1):75-80.
2007: Merja Niemi; Sirpa Jylhä; Marja-Leena Laukkanen; Hans Söderlund; Soili Mäkinen-Kiljunen; Johanna M Kallio; Nina Hakulinen; Tari Haahtela; Kristiina Takkinen; Juha Rouvinen
Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen.
Structure (London, England : 1993) 2007;15(11):1413-21.
2007: Mikko Purmonen; Jarkko Valjakka; Kristiina Takkinen; Tuomo Laitinen; Juha Rouvinen
Molecular dynamics studies on the thermostability of family 11 xylanases.
Protein engineering, design & selection : PEDS 2007;20(11):551-9.
2007: Johanna M Kallio; Markus B Linder; Juha Rouvinen
Crystal structures of hydrophobin HFBII in the presence of detergent implicate the formation of fibrils and monolayer films.
The Journal of biological chemistry 2007;282(39):28733-9.
2007: Tarja Parkkinen; Anu Koivula; Jari Vehmaanperä; Juha Rouvinen
Preliminary X-ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 9):754-7.
2007: Janne Jänis; Petri Pulkkinen; Juha Rouvinen; Pirjo Vainiotalo
Determination of steady-state kinetic parameters for a xylanase-catalyzed hydrolysis of neutral underivatized xylooligosaccharides by mass spectrometry.
Analytical biochemistry 2007;365(2):165-73.
2006: Nina Hakulinen; Kristiina Kruus; Anu Koivula; Juha Rouvinen
A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase.
Biochemical and biophysical research communications 2006;350(4):929-34.
2006: Johanna Hakanpää; Géza R Szilvay; Heidi Kaljunen; Mirko Maksimainen; Markus Linder; Juha Rouvinen
Two crystal structures of Trichoderma reesei hydrophobin HFBI--the structure of a protein amphiphile with and without detergent interaction.
Protein science : a publication of the Protein Society 2006;15(9):2129-40.
2006: Tuija Teerinen; Jarkko Valjakka; Juha Rouvinen; Kristiina Takkinen
Structure-based stability engineering of the mouse IgG1 Fab fragment by modifying constant domains.
Journal of molecular biology 2006;361(4):687-97.
2006: Johanna Hakanpää; Markus Linder; Alexander Popov; Andrea Schmidt; Juha Rouvinen
Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 A.
Acta crystallographica. Section D, Biological crystallography 2006;62(Pt 4):356-67.
2006: Tarja Parkkinen; Tarja K Nevanen; Anu Koivula; Juha Rouvinen
Crystal structures of an enantioselective fab-fragment in free and complex forms.
Journal of molecular biology 2006;357(2):471-80.
2005: Janne Jänis; Johanna Hakanpää; Nina Hakulinen; Farid M Ibatullin; Antuan Hoxha; Peter J Derrick; Juha Rouvinen; Pirjo Vainiotalo
Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography.
The FEBS journal 2005;272(9):2317-33.
2004: Johanna Karimäki; Tarja Parkkinen; Harri Santa; Ossi Pastinen; Matti Leisola; Juha Rouvinen; Ossi Turunen
Engineering the substrate specificity of xylose isomerase.
Protein engineering, design & selection : PEDS 2004;17(12):861-9.
2004: Janne Jänis; Ossi Turunen; Matti Leisola; Peter J Derrick; Juha Rouvinen; Pirjo Vainiotalo
Characterization of mutant xylanases using fourier transform ion cyclotron resonance mass spectrometry: stabilizing contributions of disulfide bridges and N-terminal extensions.
Biochemistry 2004;43(29):9556-66.
2004: Tarja Parkkinen; Nina Hakulinen; Maija Tenkanen; Matti Siika-Aho; Juha Rouvinen
Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 3):542-4.
2004: Janna Saarela; Carita Oinonen; Anu Jalanko; Juha Rouvinen; Leena Peltonen
Autoproteolytic activation of human aspartylglucosaminidase.
The Biochemical journal 2004;378(Pt 2):363-71.
2004: Johanna Hakanpää; Arja Paananen; Sanna Askolin; Tiina Nakari-Setälä; Tarja Parkkinen; Merja Penttilä; Markus B Linder; Juha Rouvinen
Atomic resolution structure of the HFBII hydrophobin, a self-assembling amphiphile.
The Journal of biological chemistry 2004;279(1):534-9.
2004: Maarit Jokela; Anitta Eskelinen; Helmut Pospiech; Juha Rouvinen; Juhani E Syväoja
Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D.
Nucleic acids research 2004;32(8):2430-40.
2004: Johanna Hakanpaa; Tarja Parkkinen; Nina Hakulinen; Markus Linder; Juha Rouvinen
Crystallization and preliminary X-ray characterization of Trichoderma reesei hydrophobin HFBII.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 1):163-5.
2003: Tuomo Laitinen; Juha Rouvinen; Mikael Peräkylä
MM-PBSA free energy analysis of endo-1,4-xylanase II (XynII)-substrate complexes: binding of the reactive sugar in a skew boat and chair conformation.
Organic & biomolecular chemistry 2003;1(20):3535-40.
2003: Jarkko Valjakka; Juha Rouvinen
Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 4):765-8.
2003: Nina Hakulinen; Ossi Turunen; Janne Jänis; Matti Leisola; Juha Rouvinen
Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Comparison of twelve xylanases in relation to their thermal stability.
European journal of biochemistry / FEBS 2003;270(7):1399-412.
2002: Jarkko Valjakka; Ari Hemminki; Seija Niemi; Hans Söderlund; Kristiina Takkinen; Juha Rouvinen
Crystal structure of an in vitro affinity- and specificity-matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within the variable domains.
The Journal of biological chemistry 2002;277(46):44021-7.
2002: Nina Hakulinen; Laura-Leena Kiiskinen; Kristiina Kruus; Markku Saloheimo; Arja Paananen; Anu Koivula; Juha Rouvinen
Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site.
Nature structural biology 2002;9(8):601-5.
2002: Jarkko Valjakka; Kristiina Takkinenz; Tuija Teerinen; Hans Söderlund; Juha Rouvinen
Structural insights into steroid hormone binding: the crystal structure of a recombinant anti-testosterone Fab fragment in free and testosterone-bound forms.
The Journal of biological chemistry 2002;277(6):4183-90.
2002: Marjaana Nousiainen; Peter J Derrick; Mari T Kaartinen; Pekka H Mäenpää; Juha Rouvinen; Pirjo Vainiotalo
A mass spectrometric study of metal binding to osteocalcin.
Chemistry & biology 2002;9(2):195-202.
2001: M Suihko; Markku Ahlgrén; Paula Aulaskari; Juha Rouvinen
Improved synthesis and characterization of 1,3,4,6-tetra-O-acetyl-2-(N-acetylacetamido)-2-deoxy-beta-D-glucopyranose.
Carbohydrate research 2001;334(4):337-41.
2001: Ossi Turunen; K Etuaho; Fred Fenel; Jari Vehmaanperä; X Wu; Juha Rouvinen; Matti Leisola
A combination of weakly stabilizing mutations with a disulfide bridge in the alpha-helix region of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermal stability through synergism.
Journal of biotechnology 2001;88(1):37-46.
2001: Janne Jänis; Juha Rouvinen; Matti Leisola; Ossi Turunen; Pirjo Vainiotalo
Thermostability of endo-1,4-beta-xylanase II from Trichoderma reesei studied by electrospray ionization Fourier-transform ion cyclotron resonance MS, hydrogen/deuterium-exchange reactions and dynamic light scattering.
The Biochemical journal 2001;356(Pt 2):453-60.
2001: Juha Rouvinen; Tuomas Virtanen; Rauno Mäntyjärvi
Search for the determinants of allergenicity in proteins of the lipocalin family.
Journal of chromatography. B, Biomedical sciences and applications 2001;756(1-2):199-206.
2001: Janna Saarela; M Laine; Carita Oinonen; C von Schantz; Anu Jalanko; Juha Rouvinen; Leena Peltonen
Molecular pathogenesis of a disease: structural consequences of aspartylglucosaminuria mutations.
Human molecular genetics 2001;10(9):983-95.
2001: Pekka H Mäenpää; Sami Väisänen; Tiina Jääskeläinen; Sanna Ryhänen; Juha Rouvinen; C Duchier; Anitta Mahonen
Vitamin D(3) analogs (MC 1288, KH 1060, EB 1089, GS 1558, and CB 1093): studies on their mechanism of action.
Steroids 2001;66(3-5):223-5.
2000: Nina Hakulinen; Maija Tenkanen; Juha Rouvinen
Three-dimensional structure of the catalytic core of acetylxylan esterase from Trichoderma reesei: insights into the deacetylation mechanism.
Journal of structural biology 2000;132(3):180-90.
2000: Carita Oinonen; Juha Rouvinen
Structural comparison of Ntn-hydrolases.
Protein science : a publication of the Protein Society 2000;9(12):2329-37.
2000: Tuomo Laitinen; Juha Rouvinen; Mikael Peräkylä
Inversion of the roles of the nucleophile and acid/base catalysts in the covalent binding of epoxyalkyl xyloside inhibitor to the catalytic glutamates of endo-1,4-beta-xylanase (XYNII): a molecular dynamics study.
Protein engineering 2000;13(4):247-52.
2000: Nina Hakulinen; S Paavilainen; Timo Korpela; Juha Rouvinen
The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form long polymeric assemblies.
Journal of structural biology 2000;129(1):69-79.
2000: Jarkko Valjakka; A Hemminki; Tuija Teerinen; Kristiina Takkinen; Juha Rouvinen
X-ray studies of recombinant anti-testosterone Fab fragments: the use of PEG 3350 in crystallization.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 2):218-21.
1999: Sami Väisänen; C Duchier; Juha Rouvinen; Pekka H Mäenpää
The importance of the putative helices 4 and 5 of human vitamin D(3) receptor for conformation and ligand binding.
Biochemical and biophysical research communications 1999;264(2):478-82.
1999: Juha Rouvinen; Jaakko Rautiainen; Tuomas Virtanen; Thomas Zeiler; Juha Kauppinen; Antti Taivainen; Rauno Mäntyjärvi
Probing the molecular basis of allergy. three-dimensional structure of the bovine lipocalin allergen Bos d 2.
The Journal of biological chemistry 1999;274(4):2337-43.
1998: Sami Väisänen; Juha Rouvinen; Pekka H Mäenpää
Putative helices 3 and 5 of the human vitamin D3 receptor are important for the binding of calcitriol.
FEBS letters 1998;440(1-2):203-7.
1998: Janna Saarela; M Laine; R Tikkanen; Carita Oinonen; Anu Jalanko; Juha Rouvinen; Leena Peltonen
Activation and oligomerization of aspartylglucosaminidase.
The Journal of biological chemistry 1998;273(39):25320-8.
1998: Jaakko Rautiainen; Seppo Auriola; Juha Rouvinen; Juha Kauppinen; Thomas Zeiler; D Novikov; Tuomas Virtanen; Rauno Mäntyjärvi
Molecular and crystal properties of Bos d 2, an allergenic protein of the lipocalin family.
Biochemical and biophysical research communications 1998;247(3):746-50.
1998: Anu Koivula; T Kinnari; V Harjunpää; Laura Ruohonen; A Teleman; T Drakenberg; Juha Rouvinen; T Alwyn Jones; Tuula T Teeri
Tryptophan 272: an essential determinant of crystalline cellulose degradation by Trichoderma reesei cellobiohydrolase Cel6A.
FEBS letters 1998;429(3):341-6.
1998: Juha Muilu; A Törrönen; Mikael Peräkylä; Juha Rouvinen
Functional conformational changes of endo-1,4-xylanase II from Trichoderma reesei: a molecular dynamics study.
Proteins 1998;31(4):434-44.
1998: Nina Hakulinen; Maija Tenkanen; Juha Rouvinen
Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reesei.
Acta crystallographica. Section D, Biological crystallography 1998;54(Pt 3):430-2.
1997: R Tikkanen; M Peltola; Carita Oinonen; Juha Rouvinen; Leena Peltonen
Several cooperating binding sites mediate the interaction of a lysosomal enzyme with phosphotransferase.
The EMBO journal 1997;16(22):6684-93.
1997: A Törrönen; Juha Rouvinen
Structural and functional properties of low molecular weight endo-1,4-beta-xylanases.
Journal of biotechnology 1997;57(1-3):137-49.
1996: A Riikonen; Juha Rouvinen; R Tikkanen; Ilkka Julkunen; Leena Peltonen; Anu Jalanko
Primary folding of aspartylglucosaminidase. Significance of disulfide bridges and evidence of early multimerization.
The Journal of biological chemistry 1996;271(35):21340-4.
1996: R Havukainen; A Törrönen; T Laitinen; Juha Rouvinen
Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei.
Biochemistry 1996;35(29):9617-24.
1996: R Tikkanen; Juha Rouvinen; A Törrönen; N Kalkkinen; Leena Peltonen
Large-scale purification and preliminary x-ray diffraction studies of human aspartylglucosaminidase.
Proteins 1996;24(2):253-8.
1995: Carita Oinonen; R Tikkanen; Juha Rouvinen; Leena Peltonen
Three-dimensional structure of human lysosomal aspartylglucosaminidase.
Nature structural biology 1995;2(12):1102-8.
1995: A Törrönen; Juha Rouvinen
Structural comparison of two major endo-1,4-xylanases from Trichoderma reesei.
Biochemistry 1995;34(3):847-56.
1994: Z Luo; Juha Rouvinen; Pekka H Mäenpää
A peptide C-terminal to the second Zn finger of human vitamin D receptor is able to specify nuclear localization.
European journal of biochemistry / FEBS 1994;223(2):381-7.
1994: A Törrönen; A Harkki; Juha Rouvinen
Three-dimensional structure of endo-1,4-beta-xylanase II from Trichoderma reesei: two conformational states in the active site.
The EMBO journal 1994;13(11):2493-501.
1993: A Törrönen; Juha Rouvinen; M Ahlgren; A Harkki; K Visuri
Crystallization and preliminary X-ray analysis of two major xylanases from Trichoderma reesei.
Journal of molecular biology 1993;233(2):313-6.
1993: Juha Rouvinen; A M Hoffrén; A Karjalainen; T A Pakkanen
Modelling of alpha 2-adrenergic ligands.
Drug design and discovery 1993;10(4):285-95.
1990: Jukka Gynther; H Konschin; H Tylli; Juha Rouvinen
Electrostatic requirements for high benzodiazepine receptor affinity among betacarboline-3-carboxylic acid derivatives.
Acta pharmaceutica Nordica 1990;2(1):45-52.