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John Blanchard

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Clarissa M Czekster; John S Blanchard
One substrate, five products: reactions catalyzed by the dihydroneopterin aldolase from Mycobacterium tuberculosis.
Hua Xu; Saugata Hazra; John S Blanchard
NXL104 irreversibly inhibits the β-lactamase from Mycobacterium tuberculosis.
Clarissa M Czekster; An Vandemeulebroucke; John S Blanchard
Two parallel pathways in the kinetic sequence of the dihydrofolate reductase from Mycobacterium tuberculosis.

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All Publications

2012: Clarissa M Czekster; John S Blanchard
One substrate, five products: reactions catalyzed by the dihydroneopterin aldolase from Mycobacterium tuberculosis.
Journal of the American Chemical Society 2012;134(48):19758-71.
2012: Hua Xu; Saugata Hazra; John S Blanchard
NXL104 irreversibly inhibits the β-lactamase from Mycobacterium tuberculosis.
Biochemistry 2012;51(22):4551-7.
2011: Clarissa M Czekster; An Vandemeulebroucke; John S Blanchard
Two parallel pathways in the kinetic sequence of the dihydrofolate reductase from Mycobacterium tuberculosis.
Biochemistry 2011;50(32):7045-56.
2011: Christine E Quartararo; John S Blanchard
Kinetic and chemical mechanism of malate synthase from Mycobacterium tuberculosis.
Biochemistry 2011;50(32):6879-87.
2011: Matthew W Vetting; Minghua Wang; Subray S Hegde; David C Hooper; George A Jacoby; John S Blanchard
Structure of QnrB1, a plasmid-mediated fluoroquinolone resistance factor.
The Journal of biological chemistry 2011;286(28):25265-73.
2011: Hua Xu; Subray S Hegde; John S Blanchard
Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP.
Biochemistry 2011;50(26):5883-92.
2011: Matthew W Vetting; Subray S Hegde; Yong Zhang; John S Blanchard
Pentapeptide-repeat proteins that act as topoisomerase poison resistance factors have a common dimer interface.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 3):296-302.
2011: Clarissa M Czekster; An Vandemeulebroucke; John S Blanchard
Kinetic and chemical mechanism of the dihydrofolate reductase from Mycobacterium tuberculosis.
Biochemistry 2011;50(3):367-75.
2011: Subray S Hegde; Matthew W Vetting; Lesley A Mitchenall; Anthony Maxwell; John S Blanchard
Structural and biochemical analysis of the pentapeptide repeat protein EfsQnr, a potent DNA gyrase inhibitor.
Antimicrobial agents and chemotherapy 2011;55(1):110-7.
2010: Lee W Tremblay; Hua Xu; John S Blanchard
Structures of the Michaelis complex (1.2 Å) and the covalent acyl intermediate (2.0 Å) of cefamandole bound in the active sites of the Mycobacterium tuberculosis β-lactamase K73A and E166A mutants.
Biochemistry 2010;49(45):9685-7.
2010: Derrick R Seiner; Subray S Hegde; John S Blanchard
Kinetics and inhibition of nicotinamidase from Mycobacterium tuberculosis.
Biochemistry 2010;49(44):9613-9.
2010: Matthew W Vetting; Subray S Hegde; John S Blanchard
The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase.
Nature chemical biology 2010;6(11):797-9.
2010: Clifton E Barry; John S Blanchard
The chemical biology of new drugs in the development for tuberculosis.
Current opinion in chemical biology 2010;14(4):456-66.
2010: Patrick A Frantom; James K Coward; John S Blanchard
UDP-(5F)-GlcNAc acts as a slow-binding inhibitor of MshA, a retaining glycosyltransferase.
Journal of the American Chemical Society 2010;132(19):6626-7.
2010: Alison L Sikora; Daniel J Wilson; Courtney C Aldrich; John S Blanchard
Kinetic and inhibition studies of dihydroxybenzoate-AMP ligase from Escherichia coli.
Biochemistry 2010;49(17):3648-57.
2010: Lee W Tremblay; Fan Fan; John S Blanchard
Biochemical and structural characterization of Mycobacterium tuberculosis beta-lactamase with the carbapenems ertapenem and doripenem.
Biochemistry 2010;49(17):3766-73.
2009: Martin Noble; John S Blanchard
Catalysis and regulation.
Current opinion in structural biology 2009;19(6):641-2.
2009: Alison L Sikora; Sean M Cahill; John S Blanchard
Enterobactin synthetase-catalyzed formation of P(1),P(3)-diadenosine-5'-tetraphosphate.
Biochemistry 2009;48(46):10827-9.
2009: L W Tremblay; John S Blanchard
The 1.9 A structure of the branched-chain amino-acid transaminase (IlvE) from Mycobacterium tuberculosis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 11):1071-7.
2009: Fan Fan; Matthew W Vetting; Patrick A Frantom; John S Blanchard
Structures and mechanisms of the mycothiol biosynthetic enzymes.
Current opinion in chemical biology 2009;13(4):451-9.
2009: Patrick A Frantom; Hui-Min Zhang; Mark R Emmett; Alan G Marshall; John S Blanchard
Mapping of the allosteric network in the regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis by the feedback inhibitor L-leucine: solution-phase H/D exchange monitored by FT-ICR mass spectrometry.
Biochemistry 2009;48(31):7457-64.
2009: Fan Fan; John S Blanchard
Toward the catalytic mechanism of a cysteine ligase (MshC) from Mycobacterium smegmatis: an enzyme involved in the biosynthetic pathway of mycothiol.
Biochemistry 2009;48(30):7150-9.
2009: Ronit Reich-Slotky; Christina A Kabbash; Phyllis Della-Latta; John S Blanchard; Steven J Feinmark; Sherry Freeman; Gilla Kaplan; Howard A Shuman; Samuel C. Silverstein
Gemfibrozil inhibits Legionella pneumophila and Mycobacterium tuberculosis enoyl coenzyme A reductases and blocks intracellular growth of these bacteria in macrophages.
Journal of bacteriology 2009;191(16):5262-71.
2009: Matthew W Vetting; Subray S Hegde; John S Blanchard
Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.
Acta crystallographica. Section D, Biological crystallography 2009;65(Pt 5):462-9.
2009: Luiz Pedro Carvalho; Patrick A Frantom; Argyrides Argyrou; John S Blanchard
Kinetic evidence for interdomain communication in the allosteric regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis.
Biochemistry 2009;48(9):1996-2004.
2009: Jean-Emmanuel Hugonnet; Lee W Tremblay; Helena I Boshoff; Clifton E Barry; John S Blanchard
Meropenem-clavulanate is effective against extensively drug-resistant Mycobacterium tuberculosis.
Science (New York, N.Y.) 2009;323(5918):1215-8.
2009: Vincent S Stoll; John S Blanchard
Buffers: principles and practice.
Methods in enzymology 2009;463():43-56.
2008: Sergei Khrapunov; Huiyong Cheng; Subray S Hegde; John S Blanchard; Michael Brenowitz
Solution structure and refolding of the Mycobacterium tuberculosis pentapeptide repeat protein MfpA.
The Journal of biological chemistry 2008;283(52):36290-9.
2008: L W Tremblay; F Fan; Matthew W Vetting; John S Blanchard
The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway.
Biochemistry 2008;47(50):13326-35.
2008: Matthew W Vetting; James Errey; John S Blanchard
Rv0802c from Mycobacterium tuberculosis: the first structure of a succinyltransferase with the GNAT fold.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 11):978-85.
2008: Alison L Sikora; Brenda A Frankel; John S Blanchard
Kinetic and chemical mechanism of arylamine N-acetyltransferase from Mycobacterium tuberculosis.
Biochemistry 2008;47(40):10781-9.
2008: Matthew W Vetting; David C Bareich; Michael Yu; John S Blanchard
Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18.
Protein science : a publication of the Protein Society 2008;17(10):1781-90.
2008: Matthew W Vetting; Chi Hye Park; Subray S Hegde; George Jacoby; David C Hooper; John S Blanchard
Mechanistic and structural analysis of aminoglycoside N-acetyltransferase AAC(6')-Ib and its bifunctional, fluoroquinolone-active AAC(6')-Ib-cr variant.
Biochemistry 2008;47(37):9825-35.
2008: Brenda A Frankel; John S Blanchard
Mechanistic analysis of Mycobacterium tuberculosis Rv1347c, a lysine Nepsilon-acyltransferase involved in mycobactin biosynthesis.
Archives of biochemistry and biophysics 2008;477(2):259-66.
2008: Maria L B Magalhães; Argyrides Argyrou; Sean M Cahill; John S Blanchard
Kinetic and mechanistic analysis of the Escherichia coli ribD-encoded bifunctional deaminase-reductase involved in riboflavin biosynthesis.
Biochemistry 2008;47(24):6499-507.
2008: Matthew W Vetting; Patrick A Frantom; John S Blanchard
Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis.
The Journal of biological chemistry 2008;283(23):15834-44.
2008: Lee W Tremblay; Jean-Emmanuel Hugonnet; John S Blanchard
Structure of the covalent adduct formed between Mycobacterium tuberculosis beta-lactamase and clavulanate.
Biochemistry 2008;47(19):5312-6.
2008: LaKenya Williams; Fan Fan; John S Blanchard; Frank M Raushel
Positional isotope exchange analysis of the Mycobacterium smegmatis cysteine ligase (MshC).
Biochemistry 2008;47(16):4843-50.
2008: Maria L B Magalhães; Matthew W Vetting; Feng Gao; Lee Freiburger; Karine Auclair; John S Blanchard
Kinetic and structural analysis of bisubstrate inhibition of the Salmonella enterica aminoglycoside 6'-N-acetyltransferase.
Biochemistry 2008;47(2):579-84.
2007: Bindu Pillai; Maia M Cherney; Christopher M Diaper; Andrew Sutherland; John S Blanchard; John C Vederas; Michael N G James
Dynamics of catalysis revealed from the crystal structures of mutants of diaminopimelate epimerase.
Biochemical and biophysical research communications 2007;363(3):547-53.
2007: Jean-Emmanuel Hugonnet; John S Blanchard
Irreversible inhibition of the Mycobacterium tuberculosis beta-lactamase by clavulanate.
Biochemistry 2007;46(43):11998-2004.
2007: Fan Fan; Andreas Luxenburger; Gavin F Painter; John S Blanchard
Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).
Biochemistry 2007;46(40):11421-9.
2007: Argyrides Argyrou; Matthew W Vetting; John S Blanchard
New insight into the mechanism of action of and resistance to isoniazid: interaction of Mycobacterium tuberculosis enoyl-ACP reductase with INH-NADP.
Journal of the American Chemical Society 2007;129(31):9582-3.
2007: Subray S Hegde; Jonathan Chandler; Matthew W Vetting; Michael Yu; John S Blanchard
Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase.
Biochemistry 2007;46(24):7187-95.
2007: Matthew W Vetting; Subray S Hegde; Keith Z Hazleton; John S Blanchard
Structural characterization of the fusion of two pentapeptide repeat proteins, Np275 and Np276, from Nostoc punctiforme: resurrection of an ancestral protein.
Protein science : a publication of the Protein Society 2007;16(4):755-60.
2006: Michael Yu; Maria L B Magalhães; Paul F Cook; John S Blanchard
Bisubstrate inhibition: Theory and application to N-acetyltransferases.
Biochemistry 2006;45(49):14788-94.
2006: Michael Yu; Luiz Pedro Carvalho; Guangxing Sun; John S Blanchard
Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-coenzyme A to identify protein substrates.
Journal of the American Chemical Society 2006;128(48):15356-7.
2006: Argyrides Argyrou; Lianji Jin; Linda Siconilfi-Baez; Ruth Hogue Angeletti; John S Blanchard
Proteome-wide profiling of isoniazid targets in Mycobacterium tuberculosis.
Biochemistry 2006;45(47):13947-53.
2006: corin wing; James Errey; Balaram Mukhopadhyay; John S Blanchard; Robert A Field
Expression and initial characterization of WbbI, a putative D-Galf:alpha-D-Glc beta-1,6-galactofuranosyltransferase from Escherichia coli K-12.
Organic & biomolecular chemistry 2006;4(21):3945-50.
2006: Rafael Silva; Luiz Pedro Carvalho; John S Blanchard; Diogenes Santos; Luiz Augusto Basso
Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein (ACP) reductase: kinetic and chemical mechanisms.
Biochemistry 2006;45(43):13064-73.
2006: Luiz Pedro Carvalho; John S Blanchard
Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis.
Biochemistry 2006;45(29):8988-99.
2006: Luiz Pedro Carvalho; John S Blanchard
Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase.
Archives of biochemistry and biophysics 2006;451(2):141-8.
2006: Bindu Pillai; Maia M Cherney; Christopher M Diaper; Andrew Sutherland; John S Blanchard; John C Vederas; Michael N G James
Structural insights into stereochemical inversion by diaminopimelate epimerase: an antibacterial drug target.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(23):8668-73.
2006: Argyrides Argyrou; Matthew W Vetting; Bola Aladegbami; John S Blanchard
Mycobacterium tuberculosis dihydrofolate reductase is a target for isoniazid.
Nature structural & molecular biology 2006;13(5):408-13.
2006: James Errey; John S Blanchard
Functional annotation and kinetic characterization of PhnO from Salmonella enterica.
Biochemistry 2006;45(9):3033-9.
2006: Matthew W Vetting; Michael Yu; Phillip M Rendle; John S Blanchard
The substrate-induced conformational change of Mycobacterium tuberculosis mycothiol synthase.
The Journal of biological chemistry 2006;281(5):2795-802.
2006: Matthew W Vetting; Subray S Hegde; Jorge Fajardo; András Fiser; Steven L Roderick; Howard Takiff; John S Blanchard
Pentapeptide repeat proteins.
Biochemistry 2006;45(1):1-10.
2005: Christopher M Diaper; Andrew Sutherland; Bindu Pillai; Michael N G James; Paul Semchuk; John S Blanchard; John C Vederas
The stereoselective synthesis of aziridine analogues of diaminopimelic acid (DAP) and their interaction with dap epimerase.
Organic & biomolecular chemistry 2005;3(24):4402-11.
2005: Maria L B Magalhaes; John S Blanchard
The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli.
Biochemistry 2005;44(49):16275-83.
2005: John S Blanchard
Old approach yields new antibiotic.
Nature medicine 2005;11(10):1045-6.
2005: Luiz Pedro Carvalho; Argyrides Argyrou; John S Blanchard
Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine.
Journal of the American Chemical Society 2005;127(28):10004-5.
2005: Matthew W Vetting; Luiz Pedro Carvalho; Steven L Roderick; John S Blanchard
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium.
The Journal of biological chemistry 2005;280(23):22108-14.
2005: Subray S Hegde; Matthew W Vetting; Steven L Roderick; Lesley Mitchenall; Anthony Maxwell; Howard Takiff; John S Blanchard
A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA.
Science (New York, N.Y.) 2005;308(5727):1480-3.
2005: James Errey; John S Blanchard
Functional characterization of a novel ArgA from Mycobacterium tuberculosis.
Journal of bacteriology 2005;187(9):3039-44.
2005: Sophie Magnet; John S Blanchard
Molecular insights into aminoglycoside action and resistance.
Chemical reviews 2005;105(2):477-98.
2005: Matthew W Vetting; Luiz Pedro S de Carvalho; Michael Yu; Subray S Hegde; Sophie Magnet; Steven L Roderick; John S Blanchard
Structure and functions of the GNAT superfamily of acetyltransferases.
Archives of biochemistry and biophysics 2005;433(1):212-26.
2004: Argyrides Argyrou; Matthew W Vetting; John S Blanchard
Characterization of a new member of the flavoprotein disulfide reductase family of enzymes from Mycobacterium tuberculosis.
The Journal of biological chemistry 2004;279(50):52694-702.
2004: Didier Guillemot; Stephane Bonacorsi; John S Blanchard; Philippe Weber; Sylvie Simon; Bruno Guesnon; Edouard Bingen; Claude Carbon
Amoxicillin-clavulanate therapy increases childhood nasal colonization by methicillin-susceptible Staphylococcus aureus strains producing high levels of penicillinase.
Antimicrobial agents and chemotherapy 2004;48(12):4618-23.
2004: Renjian Zheng; Tarun K Dam; Curtis Brewer; John S Blanchard
Active site residues in Mycobacterium tuberculosis pantothenate synthetase required in the formation and stabilization of the adenylate intermediate.
Biochemistry 2004;43(22):7171-8.
2004: Argyrides Argyrou; John S Blanchard
Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase.
Biochemistry 2004;43(14):4375-84.
2004: Matthew W Vetting; Sophie Magnet; Edward Nieves; Steven L Roderick; John S Blanchard
A bacterial acetyltransferase capable of regioselective N-acetylation of antibiotics and histones.
Chemistry & biology 2004;11(4):565-73.
2004: Matthew D Disney; Sophie Magnet; John S Blanchard; Peter H Seeberger
Aminoglycoside microarrays to study antibiotic resistance.
Angewandte Chemie (International ed. in English) 2004;43(12):1591-4.
2004: Sophie Magnet; John S Blanchard
Mechanistic and kinetic study of the ATP-dependent DNA ligase of Neisseria meningitidis.
Biochemistry 2004;43(3):710-7.
2004: Argyrides Argyrou; John S Blanchard
Flavoprotein disulfide reductases: advances in chemistry and function.
Progress in nucleic acid research and molecular biology 2004;78():89-142.
2003: Renjian Zheng; John S Blanchard
Substrate specificity and kinetic isotope effect analysis of the Eschericia coli ketopantoate reductase.
Biochemistry 2003;42(38):11289-96.
2003: Maurizio Cirilli; Renjian Zheng; Giovanna Scapin; John S Blanchard
The three-dimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and -NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity.
Biochemistry 2003;42(36):10644-50.
2003: Ryan N Vogt; Daniel J Steenkamp; Renjian Zheng; John S Blanchard
The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase.
The Biochemical journal 2003;374(Pt 3):657-66.
2003: Matthew W Vetting; Steven L Roderick; Michael Yu; John S Blanchard
Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases.
Protein science : a publication of the Protein Society 2003;12(9):1954-9.
2003: Subray S Hegde; John S Blanchard
Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase).
The Journal of biological chemistry 2003;278(25):22861-7.
2003: Matthew W Vetting; Steven L Roderick; Subray S Hegde; Sophie Magnet; John S Blanchard
What can structure tell us about in vivo function? The case of aminoglycoside-resistance genes.
Biochemical Society transactions 2003;31(Pt 3):520-2.
2003: LaKenya Williams; Renjian Zheng; John S Blanchard; Frank M Raushel
Positional isotope exchange analysis of the pantothenate synthetase reaction.
Biochemistry 2003;42(17):5108-13.
2003: Sophie Magnet; Terry-Ann Smith; Renjian Zheng; patrice nordmann; John S Blanchard
Aminoglycoside resistance resulting from tight drug binding to an altered aminoglycoside acetyltransferase.
Antimicrobial agents and chemotherapy 2003;47(5):1577-83.
2003: Argyrides Argyrou; Guangxing Sun; Bruce A Palfey; John S Blanchard
Catalysis of diaphorase reactions by Mycobacterium tuberculosis lipoamide dehydrogenase occurs at the EH4 level.
Biochemistry 2003;42(7):2218-28.
2003: John S Blanchard
Resisting bacterial drug resistance.
Chemistry & biology 2003;10(2):104-6.
2003: Michele Sugantino; Renjian Zheng; Michael Yu; John S Blanchard
Mycobacterium tuberculosis ketopantoate hydroxymethyltransferase: tetrahydrofolate-independent hydroxymethyltransferase and enolization reactions with alpha-keto acids.
Biochemistry 2003;42(1):191-9.
2002: Argyrides Argyrou; John S Blanchard; Bruce A Palfey
The lipoamide dehydrogenase from Mycobacterium tuberculosis permits the direct observation of flavin intermediates in catalysis.
Biochemistry 2002;41(49):14580-90.
2002: E K Schroeder; N de Souza; Diogenes Santos; John S Blanchard; Luiz Augusto Basso
Drugs that inhibit mycolic acid biosynthesis in Mycobacterium tuberculosis.
Current pharmaceutical biotechnology 2002;3(3):197-225.
2002: Matthew W Vetting; Subray S Hegde; Farah Javid-Majd; John S Blanchard; Steven L Roderick
Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates.
Nature structural biology 2002;9(9):653-8.
2002: Subray S Hegde; Tarun K Dam; Curtis Brewer; John S Blanchard
Thermodynamics of aminoglycoside and acyl-coenzyme A binding to the Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase.
Biochemistry 2002;41(23):7519-27.
2002: Todd W Beaman; Kurt W Vogel; Dale G Drueckhammer; John S Blanchard; Steven L Roderick
Acyl group specificity at the active site of tetrahydridipicolinate N-succinyltransferase.
Protein science : a publication of the Protein Society 2002;11(4):974-9.
2001: Subray S Hegde; Farah Javid-Majd; John S Blanchard
Overexpression and mechanistic analysis of chromosomally encoded aminoglycoside 2'-N-acetyltransferase (AAC(2')-Ic) from Mycobacterium tuberculosis.
The Journal of biological chemistry 2001;276(49):45876-81.
2001: Renjian Zheng; John S Blanchard
Steady-state and pre-steady-state kinetic analysis of Mycobacterium tuberculosis pantothenate synthetase.
Biochemistry 2001;40(43):12904-12.
2001: Argyrides Argyrou; John S Blanchard
Mycobacterium tuberculosis lipoamide dehydrogenase is encoded by Rv0462 and not by the lpdA or lpdB genes.
Biochemistry 2001;40(38):11353-63.
2001: Wuxian Shi; Luiz A Basso; Diogenes Santos; Peter C Tyler; Richard H Furneaux; John S Blanchard; Steven C Almo; Vern L Schramm
Structures of purine nucleoside phosphorylase from Mycobacterium tuberculosis in complexes with immucillin-H and its pieces.
Biochemistry 2001;40(28):8204-15.
2001: Luiz A Basso; Diogenes Santos; Wuxian Shi; Richard H Furneaux; Peter C Tyler; Vern L Schramm; John S Blanchard
Purine nucleoside phosphorylase from Mycobacterium tuberculosis. Analysis of inhibition by a transition-state analogue and dissection by parts.
Biochemistry 2001;40(28):8196-203.
2001: MP Patel; John S Blanchard
Mycobacterium tuberculosis mycothione reductase: pH dependence of the kinetic parameters and kinetic isotope effects.
Biochemistry 2001;40(17):5119-26.
2001: Sophie Magnet; Thierry Lambert; Patrice Courvalin; John S Blanchard
Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase.
Biochemistry 2001;40(12):3700-9.
2001: A M Paiva; Dana E Vanderwall; John S Blanchard; John W Kozarich; Joanne M Williamson; Theresa M Kelly
Inhibitors of dihydrodipicolinate reductase, a key enzyme of the diaminopimelate pathway of Mycobacterium tuberculosis.
Biochimica et biophysica acta 2001;1545(1-2):67-77.
2000: Renjian Zheng; John S Blanchard
Identification of active site residues in E. coli ketopantoate reductase by mutagenesis and chemical rescue.
Biochemistry 2000;39(51):16244-51.
2000: J F Caplan; Renjian Zheng; John S Blanchard; John C Vederas
Vinylogous amide analogues of diaminopimelic acid (DAP) as inhibitors of enzymes involved in bacterial lysine biosynthesis.
Organic letters 2000;2(24):3857-60.
2000: J N Barlow; John S Blanchard
Enzymatic synthesis of UDP-(3-deoxy-3-fluoro)-D-galactose and UDP-(2-deoxy-2-fluoro)-D-galactose and substrate activity with UDP-galactopyranose mutase.
Carbohydrate research 2000;328(4):473-80.
2000: Maurizio Cirilli; Giovanna Scapin; Andrew Sutherland; John C Vederas; John S Blanchard
The three-dimensional structure of the ternary complex of Corynebacterium glutamicum diaminopimelate dehydrogenase-NADPH-L-2-amino-6-methylene-pimelate.
Protein science : a publication of the Protein Society 2000;9(10):2034-7.
2000: N M Brunhuber; James B Thoden; John S Blanchard; Janeen L Vanhooke
Rhodococcus L-phenylalanine dehydrogenase: kinetics, mechanism, and structural basis for catalytic specificity.
Biochemistry 2000;39(31):9174-87.
2000: TL Born; M Franklin; John S Blanchard
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Haemophilus influenzae met2-encoded homoserine transacetylase.
Biochemistry 2000;39(29):8556-64.
2000: Renjian Zheng; John S Blanchard
Kinetic and mechanistic analysis of the E. coli panE-encoded ketopantoate reductase.
Biochemistry 2000;39(13):3708-17.
2000: Farah Javid-Majd; John S Blanchard
Mechanistic analysis of the argE-encoded N-acetylornithine deacetylase.
Biochemistry 2000;39(6):1285-93.
1999: TL Born; John S Blanchard
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase.
Biochemistry 1999;38(43):14416-23.
1999: TL Born; John S Blanchard
Structure/function studies on enzymes in the diaminopimelate pathway of bacterial cell wall biosynthesis.
Current opinion in chemical biology 1999;3(5):607-13.
1999: MP Patel; John S Blanchard
Expression, purification, and characterization of Mycobacterium tuberculosis mycothione reductase.
Biochemistry 1999;38(36):11827-33.
1999: C W Koo; John S Blanchard
Chemical mechanism of Haemophilus influenzae diaminopimelate epimerase.
Biochemistry 1999;38(14):4416-22.
1999: R Ledwidge; John S Blanchard
The dual biosynthetic capability of N-acetylornithine aminotransferase in arginine and lysine biosynthesis.
Biochemistry 1999;38(10):3019-24.
1999: Janeen L Vanhooke; James B Thoden; N M Brunhuber; John S Blanchard; Hazel M Holden
Phenylalanine dehydrogenase from Rhodococcus sp. M4: high-resolution X-ray analyses of inhibitory ternary complexes reveal key features in the oxidative deamination mechanism.
Biochemistry 1999;38(8):2326-39.
1998: Maurizio Cirilli; Renjian Zheng; Giovanna Scapin; John S Blanchard
Structural symmetry: the three-dimensional structure of Haemophilus influenzae diaminopimelate epimerase.
Biochemistry 1998;37(47):16452-8.
1998: Pan-Fen Wang; JA Marcinkeviciene; Charles H Williams; John S Blanchard
Thioredoxin reductase-thioredoxin fusion enzyme from Mycobacterium leprae: comparison with the separately expressed thioredoxin reductase.
Biochemistry 1998;37(46):16378-89.
1998: T Wilson; G W de Lisle; JA Marcinkeviciene; John S Blanchard; Desmond M Collins
Antisense RNA to ahpC, an oxidative stress defence gene involved in isoniazid resistance, indicates that AhpC of Mycobacterium bovis has virulence properties.
Microbiology (Reading, England) 1998;144 ( Pt 10)():2687-95.
1998: MP Patel; JA Marcinkeviciene; John S Blanchard
Enterococcus faecalis glutathione reductase: purification, characterization and expression under normal and hyperbaric O2 conditions.
FEMS microbiology letters 1998;166(1):155-63.
1998: L A Basso; Renjian Zheng; J M Musser; William R. Jacobs Jr.; John S Blanchard
Mechanisms of isoniazid resistance in Mycobacterium tuberculosis: enzymatic characterization of enoyl reductase mutants identified in isoniazid-resistant clinical isolates.
The Journal of infectious diseases 1998;178(3):769-75.
1998: TL Born; Renjian Zheng; John S Blanchard
Hydrolysis of N-succinyl-L,L-diaminopimelic acid by the Haemophilus influenzae dapE-encoded desuccinylase: metal activation, solvent isotope effects, and kinetic mechanism.
Biochemistry 1998;37(29):10478-87.
1998: Todd W Beaman; John S Blanchard; Steven L Roderick
The conformational change and active site structure of tetrahydrodipicolinate N-succinyltransferase.
Biochemistry 1998;37(29):10363-9.
1998: Giovanna Scapin; Maurizio Cirilli; S G Reddy; Y Gao; John C Vederas; John S Blanchard
Substrate and inhibitor binding sites in Corynebacterium glutamicum diaminopimelate dehydrogenase.
Biochemistry 1998;37(10):3278-85.
1998: F Wang; Giovanna Scapin; John S Blanchard; Ruth Hogue Angeletti
Substrate binding and conformational changes of Clostridium glutamicum diaminopimelate dehydrogenase revealed by hydrogen/deuterium exchange and electrospray mass spectrometry.
Protein science : a publication of the Protein Society 1998;7(2):293-9.
1998: L A Basso; John S Blanchard
Resistance to antitubercular drugs.
Advances in experimental medicine and biology 1998;456():115-44.
1998: Giovanna Scapin; John S Blanchard
Enzymology of bacterial lysine biosynthesis.
Advances in enzymology and related areas of molecular biology 1998;72():279-324.
1998: John S Blanchard
The ying and yang of rifampicin.
Nature medicine 1998;4(1):14-5.
1997: Giovanna Scapin; S G Reddy; Renjian Zheng; John S Blanchard
Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate.
Biochemistry 1997;36(49):15081-8.
1997: JA Marcinkeviciene; John S Blanchard
Catalytic properties of lipoamide dehydrogenase from Mycobacterium smegmatis.
Archives of biochemistry and biophysics 1997;340(2):168-76.
1997: F Wang; John S Blanchard; X J Tang
Hydrogen exchange/electrospray ionization mass spectrometry studies of substrate and inhibitor binding and conformational changes of Escherichia coli dihydrodipicolinate reductase.
Biochemistry 1997;36(13):3755-9.
1997: Todd W Beaman; D A Binder; John S Blanchard; Steven L Roderick
Three-dimensional structure of tetrahydrodipicolinate N-succinyltransferase.
Biochemistry 1997;36(3):489-94.
1996: Giovanna Scapin; S G Reddy; John S Blanchard
Three-dimensional structure of meso-diaminopimelic acid dehydrogenase from Corynebacterium glutamicum.
Biochemistry 1996;35(42):13540-51.
1996: S G Reddy; Giovanna Scapin; John S Blanchard
Interaction of pyridine nucleotide substrates with Escherichia coli dihydrodipicolinate reductase: thermodynamic and structural analysis of binary complexes.
Biochemistry 1996;35(41):13294-302.
1996: D A Binder; John S Blanchard; Steven L Roderick
Crystallization and preliminary crystallographic analysis of tetrahydrodipicolinate-N-succinyltransferase.
Proteins 1996;26(1):115-7.
1996: S G Reddy; Giovanna Scapin; John S Blanchard
Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum.
Proteins 1996;25(4):514-6.
1996: R Kliukiené; A Maroziené; N Cénas; Katja Becker; John S Blanchard
Photoinactivation of trypanothione reductase and glutathione reductase by Al-phthalocyanine tetrasulfonate and hematoporphyrin.
Biochemical and biophysical research communications 1996;218(2):629-32.
1996: W Tao; Charles Grubmeyer; John S Blanchard
Transition state structure of Salmonella typhimurium orotate phosphoribosyltransferase.
Biochemistry 1996;35(1):14-21.
1996: John S Blanchard
Molecular mechanisms of drug resistance in Mycobacterium tuberculosis.
Annual review of biochemistry 1996;65():215-39.
1996: James C Sacchettini; John S Blanchard
The structure and function of the isoniazid target in M. tuberculosis.
Research in microbiology 1996;147(1-2):36-43.
1995: Renjian Zheng; Narimantas Cenas; John S Blanchard
Catalytic and potentiometric characterization of E201D and E201Q mutants of Trypanosoma congolense trypanothione reductase.
Biochemistry 1995;34(39):12697-703.
1995: JA Marcinkeviciene; Richard Magliozzo; John S Blanchard
Purification and characterization of the Mycobacterium smegmatis catalase-peroxidase involved in isoniazid activation.
The Journal of biological chemistry 1995;270(38):22290-5.
1995: A Quémard; James C Sacchettini; A Dessen; C Vilcheze; R Bittman; William R. Jacobs Jr.; John S Blanchard
Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis.
Biochemistry 1995;34(26):8235-41.
1995: JA Marcinkeviciene; John S Blanchard
Quinone reductase reaction catalyzed by Streptococcus faecalis NADH peroxidase.
Biochemistry 1995;34(20):6621-7.
1995: Giovanna Scapin; John S Blanchard; James C Sacchettini
Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase.
Biochemistry 1995;34(11):3502-12.
1995: S G Reddy; James C Sacchettini; John S Blanchard
Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase.
Biochemistry 1995;34(11):3492-501.
1995: A Dessen; A Quémard; John S Blanchard; William R. Jacobs Jr.; James C Sacchettini
Crystal structure and function of the isoniazid target of Mycobacterium tuberculosis.
Science (New York, N.Y.) 1995;267(5204):1638-41.
1994: Y Tanaka; W Tao; John S Blanchard; E J Hehre
Transition state structures for the hydrolysis of alpha-D-glucopyranosyl fluoride by retaining and inverting reactions of glycosylases.
The Journal of biological chemistry 1994;269(51):32306-12.
1994: B N Leichus; John S Blanchard
Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase.
Biochemistry 1994;33(48):14642-9.
1994: Narimantas Cenas; D Bironaite; E Dickancaite; Zilvinas Anusevicius; Jonas Sarlauskas; John S Blanchard
Chinifur, a selective inhibitor and "subversive substrate" for Trypanosoma congolense trypanothione reductase.
Biochemical and biophysical research communications 1994;204(1):224-9.
1994: N M Brunhuber; A Banerjee; William R. Jacobs Jr.; John S Blanchard
Cloning, sequencing, and expression of Rhodococcus L-phenylalanine dehydrogenase. Sequence comparisons to amino-acid dehydrogenases.
The Journal of biological chemistry 1994;269(23):16203-11.
1994: Narimantas Cenas; D Arscott; C H Williams; John S Blanchard
Mechanism of reduction of quinones by Trypanosoma congolense trypanothione reductase.
Biochemistry 1994;33(9):2509-15.
1994: N M Brunhuber; John S Blanchard
The biochemistry and enzymology of amino acid dehydrogenases.
Critical reviews in biochemistry and molecular biology 1994;29(6):415-67.
1992: B N Leichus; M Bradley; KC Nadeau; C T Walsh; John S Blanchard
Kinetic isotope effect analysis of the reaction catalyzed by Trypanosoma congolense trypanothione reductase.
Biochemistry 1992;31(28):6414-20.
1992: B N Leichus; John S Blanchard
Pig heart lipoamide dehydrogenase: solvent equilibrium and kinetic isotope effects.
Biochemistry 1992;31(12):3065-72.
1991: W L Sweet; John S Blanchard
Human erythrocyte glutathione reductase: chemical mechanism and structure of the transition state for hydride transfer.
Biochemistry 1991;30(35):8702-9.
1991: V S Stoll; John S Blanchard
Chemical mechanism and rate-limiting steps in the reaction catalyzed by Streptococcus faecalis NADH peroxidase.
Biochemistry 1991;30(4):942-8.
1990: Marco Vanoni; K K Wong; David P Ballou; John S Blanchard
Glutathione reductase: comparison of steady-state and rapid reaction primary kinetic isotope effects exhibited by the yeast, spinach, and Escherichia coli enzymes.
Biochemistry 1990;29(24):5790-6.
1990: W L Sweet; John S Blanchard
Fumarase: viscosity dependence of the kinetic parameters.
Archives of biochemistry and biophysics 1990;277(1):196-202.
1990: V S Stoll; John S Blanchard
Buffers: principles and practice.
Methods in enzymology 1990;182():24-38.
1989: N Schiering; V S Stoll; John S Blanchard; Emil F Pai
Crystallization and preliminary x-ray diffraction study of the flavoprotein NADH peroxidase from Streptococcus faecalis 10C1.
The Journal of biological chemistry 1989;264(35):21144-5.
1989: H Matsui; John S Blanchard; Curtis Brewer; E J Hehre
Alpha-secondary tritium kinetic isotope effects for the hydrolysis of alpha-D-glucopyranosyl fluoride by exo-alpha-glucanases.
The Journal of biological chemistry 1989;264(15):8714-6.
1989: K K Wong; John S Blanchard
Human erythrocyte glutathione reductase: pH dependence of kinetic parameters.
Biochemistry 1989;28(8):3586-90.
1988: K K Wong; Marco Vanoni; John S Blanchard
Glutathione reductase: solvent equilibrium and kinetic isotope effects.
Biochemistry 1988;27(18):7091-6.
1988: V S Stoll; John S Blanchard
Kinetic mechanism and nucleotide specificity of NADH peroxidase.
Archives of biochemistry and biophysics 1988;260(2):752-62.
1987: J F Cubells; John S Blanchard; M H Makman
The effects of in vivo inactivation of GABA-transaminase and glutamic acid decarboxylase on levels of GABA in the rat retina.
Brain research 1987;419(1-2):208-15.
1986: J F Cubells; John S Blanchard; D M Smith; M H Makman
In vivo action of enzyme-activated irreversible inhibitors of glutamic acid decarboxylase and gamma-aminobutyric acid transaminase in retina vs. brain.
The Journal of pharmacology and experimental therapeutics 1986;238(2):508-14.
1986: L Butera; S Englard; John S Blanchard; G Avigad
Structures of D-threo-2,5-hexodiulose 1-phosphate and D-threo-2,5-hexodiulose 1,6-bisphosphate (5-keto-D-fructose mono- and bis-phosphate) in solution by 13C-N.M.R. spectroscopy.
Carbohydrate research 1986;148(2):179-88.
1985: R M Burger; John S Blanchard; Susan B. Horwitz; Jack Peisach
The redox state of activated bleomycin.
The Journal of biological chemistry 1985;260(29):15406-9.
1985: S Englard; John S Blanchard; C F Midelfort
Gamma-butyrobetaine hydroxylase: stereochemical course of the hydroxylation reaction.
Biochemistry 1985;24(5):1110-6.
1984: G A Orr; John S Blanchard
High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides.
Analytical biochemistry 1984;142(1):232-4.
1984: John S Blanchard
Buffers for enzymes.
Methods in enzymology 1984;104():404-14.
1983: John S Blanchard; S Englard
gamma-butyrobetaine hydroxylase: primary and secondary tritium kinetic isotope effects.
Biochemistry 1983;22(25):5922-9.
1982: John S Blanchard; S Englard; A Kondo
gamma-Butyrobetaine hydroxylase: a unique protective effect of catalase.
Archives of biochemistry and biophysics 1982;219(2):327-34.
1982: John S Blanchard; Curtis Brewer; S Englard; G Avigad
Solution structure of 5-keto-D-fructose: relevance to the specificity of hexose kinases.
Biochemistry 1982;21(1):75-81.
1981: A Kondo; John S Blanchard; S Englard
Purification and properties of calf liver gamma-butyrobetaine hydroxylase.
Archives of biochemistry and biophysics 1981;212(2):338-46.
1980: P F Cook; John S Blanchard; W W Cleland
Primary and secondary deuterium isotope effects on equilibrium constants for enzyme-catalyzed reactions.
Biochemistry 1980;19(21):4853-8.
1980: John S Blanchard; W W Cleland
Use of isotope effects to deduce the chemical mechanism of fumarase.
Biochemistry 1980;19(19):4506-13.
1980: John S Blanchard; W W Cleland
Kinetic and chemical mechanisms of yeast formate dehydrogenase.
Biochemistry 1980;19(15):3543-50.