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Nigel Scrutton

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Co-Publications
Name
48
Sutcliffe, Michael
38
Munro, Andrew
36
Leys, David
33
Basran, Jaswir
22
hay, sam
15
Perham, Richard
13
Bruce, Neil
13
Hothi, Parvinder
10
Heyes, Derren
9
Pudney, Christopher
9
Moody, Peter
8
Packman, Len
8
McLean, Kirsty
7
Toogood, Helen
7
Rigby, Stephen

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Publications

TOP 3
Hay Sam; Evans Rhiannon M; Levy Colin; Loveridge E Joel; Wang Xi; Leys David; Allemann Rudolf K; Scrutton Nigel S
Are the catalytic properties of enzymes from piezophilic organisms pressure adapted?
Pudney Christopher R; Hay Sam; Scrutton Nigel S
Bipartite recognition and conformational sampling mechanisms for hydride transfer from nicotinamide coenzyme to FMN in pentaerythritol tetranitrate reductase.
Brenner Sibylle; Heyes Derren J; Hay Sam; Hough Michael A; Eady Robert R; Hasnain S Samar; Scrutton Nigel S
Demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases.

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All Publications

2009: Hay Sam; Evans Rhiannon M; Levy Colin; Loveridge E Joel; Wang Xi; Leys David; Allemann Rudolf K; Scrutton Nigel S
Are the catalytic properties of enzymes from piezophilic organisms pressure adapted?
Chembiochem : a European journal of chemical biology 2009;10(14):2348-53.
2009: Pudney Christopher R; Hay Sam; Scrutton Nigel S
Bipartite recognition and conformational sampling mechanisms for hydride transfer from nicotinamide coenzyme to FMN in pentaerythritol tetranitrate reductase.
The FEBS journal 2009;276(17):4780-9.
2009: Brenner Sibylle; Heyes Derren J; Hay Sam; Hough Michael A; Eady Robert R; Hasnain S Samar; Scrutton Nigel S
Demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases.
The Journal of biological chemistry 2009;284(38):25973-83.
2009: Hay Sam; Pudney Christopher R; Scrutton Nigel S
Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling.
The FEBS journal 2009;276(15):3930-41.
2009: Dunford Adrian J; Girvan Hazel M; Scrutton Nigel S; Munro Andrew W
Probing the molecular determinants of coenzyme selectivity in the P450 BM3 FAD/NADPH domain.
Biochimica et biophysica acta 2009;1794(8):1181-9.
2009: Menon Binuraj R K; Waltho Jonathan P; Scrutton Nigel S; Heyes Derren J
Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase.
The Journal of biological chemistry 2009;284(27):18160-6.
2009: Heuts Dominic P H M; Scrutton Nigel S; McIntire William S; Fraaije Marco W
What's in a covalent bond? On the role and formation of covalently bound flavin cofactors.
The FEBS journal 2009;276(13):3405-27.
2009: Tralau Tewes; Lafite Pierre; Levy Colin; Combe John P; Scrutton Nigel S; Leys David
An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.
The Journal of biological chemistry 2009;284(26):17826-34.
2009: Pudney Christopher R; McGrory Tom; Lafite Pierre; Pang Jiayun; Hay Sam; Leys David; Sutcliffe Michael J; Scrutton Nigel S
Parallel pathways and free-energy landscapes for enzymatic hydride transfer probed by hydrostatic pressure.
Chembiochem : a European journal of chemical biology 2009;10(8):1379-84.
2009: Woodward Jonathan R; Foster Timothy J; Jones Alex R; Salaoru Adrian T; Scrutton Nigel S
Time-resolved studies of radical pairs.
Biochemical Society transactions 2009;37(Pt 2):358-62.
2009: Heyes Derren J; Scrutton Nigel S
Conformational changes in the catalytic cycle of protochlorophyllide oxidoreductase: what lessons can be learnt from dihydrofolate reductase?
Biochemical Society transactions 2009;37(Pt 2):354-7.
2009: Munro Andrew W; Scrutton Nigel S
Enzyme mechanisms: fast reaction and computational approaches.
Biochemical Society transactions 2009;37(Pt 2):333-5.
2009: Wolthers Kirsten R; Scrutton Nigel S
Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex.
The FEBS journal 2009;276(7):1942-51.
2009: Heyes Derren J; Quinn Anne-Marie; Cullis Paul M; Lee Michael; Munro Andrew W; Scrutton Nigel S
Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS).
Chemical communications (Cambridge, England) 2009;(9):1124-6.
2009: Heyes Derren J; Sakuma Michiyo; de Visser Sam P; Scrutton Nigel S
Nuclear quantum tunneling in the light-activated enzyme protochlorophyllide oxidoreductase.
The Journal of biological chemistry 2009;284(6):3762-7.
2009: Sabri Muna; Dunford Adrian J; McLean Kirsty J; Neeli Rajasekhar; Scrutton Nigel S; Leys David; Munro Andrew W
Characterization of coenzyme binding and selectivity determinants in Mycobacterium tuberculosis flavoprotein reductase A: analysis of Arg(199) and Arg(200) mutants at the NADP(H) 2'-phosphate binding site.
The Biochemical journal 2009;417(1):103-12.
2009: Heyes Derren J; Sakuma Michiyo; Scrutton Nigel S
Solvent-slaved protein motions accompany proton but not hydride tunneling in light-activated protochlorophyllide oxidoreductase.
Angewandte Chemie (International ed. in English) 2009;48(21):3850-3.
2009: Hay Sam; Pudney Christopher R; McGrory Tom A; Pang Jiayun; Sutcliffe Michael J; Scrutton Nigel S
Barrier compression enhances an enzymatic hydrogen-transfer reaction.
Angewandte Chemie (International ed. in English) 2009;48(8):1452-4.
2008: Nivinskas Henrikas; Sarlauskas Jonas; Anusevicius Zilvinas; Toogood Helen S; Scrutton Nigel S; Cenas Narimantas
Reduction of aliphatic nitroesters and N-nitramines by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships.
The FEBS journal 2008;275(24):6192-203.
2008: Wolthers Kirsten R; Rigby Stephen E J; Scrutton Nigel S
Mechanism of radical-based catalysis in the reaction catalyzed by adenosylcobalamin-dependent ornithine 4,5-aminomutase.
The Journal of biological chemistry 2008;283(50):34615-25.
2008: Hay Sam; Pudney Christopher R; Hothi Parvinder; Scrutton Nigel S
Correction of pre-steady-state KIEs for isotopic impurities and the consequences of kinetic isotope fractionation.
The journal of physical chemistry. A 2008;112(50):13109-15.
2008: Johannissen Linus O; Scrutton Nigel S; Sutcliffe Michael J
The enzyme aromatic amine dehydrogenase induces a substrate conformation crucial for promoting vibration that significantly reduces the effective potential energy barrier to proton transfer.
Journal of the Royal Society, Interface / the Royal Society 2008;5 Suppl 3():S225-32.
2008: Hothi Parvinder; Hay Sam; Roujeinikova Anna; Sutcliffe Michael J; Lee Michael; Leys David; Cullis Paul M; Scrutton Nigel S
Driving force analysis of proton tunnelling across a reactivity series for an enzyme-substrate complex.
Chembiochem : a European journal of chemical biology 2008;9(17):2839-45.
2008: Heyes Derren J; Menon Binuraj R K; Sakuma Michiyo; Scrutton Nigel S
Conformational events during ternary enzyme-substrate complex formation are rate limiting in the catalytic cycle of the light-driven enzyme protochlorophyllide oxidoreductase.
Biochemistry 2008;47(41):10991-8.
2008: Hay Sam; Scrutton Nigel S
H-transfers in Photosystem II: what can we learn from recent lessons in the enzyme community?
Photosynthesis research 2008;98(1-3):169-77.
2008: Hay Sam; Scrutton Nigel S
Incorporation of hydrostatic pressure into models of hydrogen tunneling highlights a role for pressure-modulated promoting vibrations.
Biochemistry 2008;47(37):9880-7.
2008: Brenner Sibylle; Hay Sam; Munro Andrew W; Scrutton Nigel S
Inter-flavin electron transfer in cytochrome P450 reductase - effects of solvent and pH identify hidden complexity in mechanism.
The FEBS journal 2008;275(18):4540-57.
2008: Hay Sam; Pudney Christopher R; Sutcliffe Michael J; Scrutton Nigel S
Solvent as a probe of active site motion and chemistry during the hydrogen tunnelling reaction in morphinone reductase.
Chemphyschem : a European journal of chemical physics and physical chemistry 2008;9(13):1875-81.
2008: Hay Sam; Pang Jiayun; Monaghan Phillip J; Wang Xi; Evans Rhiannon M; Sutcliffe Michael J; Allemann Rudolf K; Scrutton Nigel S
Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions.
Chemphyschem : a European journal of chemical physics and physical chemistry 2008;9(11):1536-9.
2008: Dunn Rachel V; Marshall Ker R; Munro Andrew W; Scrutton Nigel S
The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme-substrate complex.
The FEBS journal 2008;275(15):3850-8.
2008: Pang Jiayun; Hay Sam; Scrutton Nigel S; Sutcliffe Michael J
Deep tunneling dominates the biologically important hydride transfer reaction from NADH to FMN in morphinone reductase.
Journal of the American Chemical Society 2008;130(22):7092-7.
2008: Burgess Selena G; Messiha Hanan Latif; Katona Gergely; Rigby Stephen E J; Leys David; Scrutton Nigel S
Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair.
Biochemistry 2008;47(18):5168-81.
2008: Neeli Rajasekhar; Sabri Muna; McLean Kirsty J; Dunford Adrian J; Scrutton Nigel S; Leys David; Munro Andrew W
Trp(359) regulates flavin thermodynamics and coenzyme selectivity in Mycobacterium tuberculosis FprA.
The Biochemical journal 2008;411(3):563-70.
2008: Hay Sam; Pudney Christopher; Hothi Parvinder; Johannissen Linus O; Masgrau Laura; Pang Jiayun; Leys David; Sutcliffe Michael J; Scrutton Nigel S
Atomistic insight into the origin of the temperature-dependence of kinetic isotope effects and H-tunnelling in enzyme systems is revealed through combined experimental studies and biomolecular simulation.
Biochemical Society transactions 2008;36(Pt 1):16-21.
2008: Hothi Parvinder; Lee Michael; Cullis Paul M; Leys David; Scrutton Nigel S
Catalysis by the isolated tryptophan tryptophylquinone-containing subunit of aromatic amine dehydrogenase is distinct from native enzyme and synthetic model compounds and allows further probing of TTQ mechanism.
Biochemistry 2008;47(1):183-94.
2008: Hay Sam; Pudney Christopher R; Sutcliffe Michael J; Scrutton Nigel S
Are environmentally coupled enzymatic hydrogen tunneling reactions influenced by changes in solution viscosity?
Angewandte Chemie (International ed. in English) 2008;47(3):537-40.
2007: Jones Alex R; Hay Sam; Woodward Jonathan R; Scrutton Nigel S
Magnetic field effect studies indicate reduced geminate recombination of the radical pair in substrate-bound adenosylcobalamin-dependent ethanolamine ammonia lyase.
Journal of the American Chemical Society 2007;129(50):15718-27.
2007: Toogood Helen S; Leys David; Scrutton Nigel S
Dynamics driving function: new insights from electron transferring flavoproteins and partner complexes.
The FEBS journal 2007;274(21):5481-504.
2007: Pudney Christopher R; Hay Sam; Pang Jiayun; Costello Claire; Leys David; Sutcliffe Michael J; Scrutton Nigel S
Mutagenesis of morphinone reductase induces multiple reactive configurations and identifies potential ambiguity in kinetic analysis of enzyme tunneling mechanisms.
Journal of the American Chemical Society 2007;129(45):13949-56.
2007: Heyes Derren J; Sakuma Michiyo; Scrutton Nigel S
Laser excitation studies of the product release steps in the catalytic cycle of the light-driven enzyme, protochlorophyllide oxidoreductase.
The Journal of biological chemistry 2007;282(44):32015-20.
2007: Wolthers Kirsten R; Lou Xiaodong; Toogood Helen S; Leys David; Scrutton Nigel S
Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry.
Biochemistry 2007;46(42):11833-44.
2007: Gong Min; Hay Sam; Marshall Ker R; Munro Andrew W; Scrutton Nigel S
DNA binding suppresses human AIF-M2 activity and provides a connection between redox chemistry, reactive oxygen species, and apoptosis.
The Journal of biological chemistry 2007;282(41):30331-40.
2007: Ranaghan Kara E; Masgrau Laura; Scrutton Nigel S; Sutcliffe Michael J; Mulholland Adrian J
Analysis of classical and quantum paths for deprotonation of methylamine by methylamine dehydrogenase.
Chemphyschem : a European journal of chemical physics and physical chemistry 2007;8(12):1816-35.
2007: Hothi Parvinder; Roujeinikova Anna; Khadra Khalid Abu; Lee Michael; Cullis Paul; Leys David; Scrutton Nigel S
Isotope effects reveal that para-substituted benzylamines are poor reactivity probes of the quinoprotein mechanism for aromatic amine dehydrogenase.
Biochemistry 2007;46(32):9250-9.
2007: Dunford Adrian J; McLean Kirsty J; Sabri Muna; Seward Harriet E; Heyes Derren J; Scrutton Nigel S; Munro Andrew W
Rapid P450 heme iron reduction by laser photoexcitation of Mycobacterium tuberculosis CYP121 and CYP51B1. Analysis of CO complexation reactions and reversibility of the P450/P420 equilibrium.
The Journal of biological chemistry 2007;282(34):24816-24.
2007: Roujeinikova Anna; Hothi Parvinder; Masgrau Laura; Sutcliffe Michael J; Scrutton Nigel S; Leys David
New insights into the reductive half-reaction mechanism of aromatic amine dehydrogenase revealed by reaction with carbinolamine substrates.
The Journal of biological chemistry 2007;282(33):23766-77.
2007: Brenner Sibylle; Hay Sam; Girvan Hazel M; Munro Andrew W; Scrutton Nigel S
Conformational dynamics of the cytochrome P450 BM3/N-palmitoylglycine complex: the proposed "proximal-distal" transition probed by temperature-jump spectroscopy.
The journal of physical chemistry. B 2007;111(27):7879-86.
2007: Wolthers Kirsten R; Scrutton Nigel S
Protein interactions in the human methionine synthase-methionine synthase reductase complex and implications for the mechanism of enzyme reactivation.
Biochemistry 2007;46(23):6696-709.
2007: Girvan Hazel M; Heyes Derren J; Scrutton Nigel S; Munro Andrew W
Laser photoexcitation of NAD(P)H induces reduction of P450 BM3 heme domain on the microsecond time scale.
Journal of the American Chemical Society 2007;129(20):6647-53.
2007: Dunford Adrian J; Rigby Stephen E J; Hay Sam; Munro Andrew W; Scrutton Nigel S
Conformational and thermodynamic control of electron transfer in neuronal nitric oxide synthase.
Biochemistry 2007;46(17):5018-29.
2007: Monaghan Phillip J; Leys David; Scrutton Nigel S
Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase/oxidase.
The FEBS journal 2007;274(8):2070-87.
2007: Masgrau Laura; Ranaghan Kara E; Scrutton Nigel S; Mulholland Adrian J; Sutcliffe Michael J
Tunneling and classical paths for proton transfer in an enzyme reaction dominated by tunneling: oxidation of tryptamine by aromatic amine dehydrogenase.
The journal of physical chemistry. B 2007;111(11):3032-47.
2007: Johannissen Linus O; Hay Sam; Scrutton Nigel S; Sutcliffe Michael J
Proton tunneling in aromatic amine dehydrogenase is driven by a short-range sub-picosecond promoting vibration: consistency of simulation and theory with experiment.
The journal of physical chemistry. B 2007;111(10):2631-8.
2007: Tejero Jesús; Peregrina José Ramón; Martínez-Júlvez Marta; Gutiérrez Aldo; Gómez-Moreno Carlos; Scrutton Nigel S; Medina Milagros
Catalytic mechanism of hydride transfer between NADP+/H and ferredoxin-NADP+ reductase from Anabaena PCC 7119.
Archives of biochemistry and biophysics 2007;459(1):79-90.
2007: Wolthers Kirsten R; Toogood Helen S; Jowitt Thomas A; Marshall Ker R; Leys David; Scrutton Nigel S
Crystal structure and solution characterization of the activation domain of human methionine synthase.
The FEBS journal 2007;274(3):738-50.
2007: Hay Sam; Sutcliffe Michael J; Scrutton Nigel S
Promoting motions in enzyme catalysis probed by pressure studies of kinetic isotope effects.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(2):507-12.
2007: Miseviciene Lina; Anusevicius Zilvinas; Sarlauskas Jonas; Harris Richard J; Scrutton Nigel S; Cenas Narimantas
Two-electron reduction of quinones by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships.
Acta biochimica Polonica 2007;54(2):379-85.
2006: Girvan H M; Waltham T N; Neeli R; Collins H F; McLean K J; Scrutton N S; Leys D; Munro A W
Flavocytochrome P450 BM3 and the origin of CYP102 fusion species.
Biochemical Society transactions 2006;34(Pt 6):1173-7.
2006: Roujeinikova Anna; Scrutton Nigel S; Leys David
Atomic level insight into the oxidative half-reaction of aromatic amine dehydrogenase.
The Journal of biological chemistry 2006;281(52):40264-72.
2006: Pudney Christopher R; Hay Sam; Sutcliffe Michael J; Scrutton Nigel S
Alpha-secondary isotope effects as probes of "tunneling-ready" configurations in enzymatic H-tunneling: insight from environmentally coupled tunneling models.
Journal of the American Chemical Society 2006;128(43):14053-8.
2006: Sutcliffe Michael J; Scrutton Nigel S
Computational studies of enzyme mechanism: linking theory with experiment in the analysis of enzymic H-tunnelling.
Physical chemistry chemical physics : PCCP 2006;8(39):4510-6.
2006: Basran Jaswir; Fullerton Stephen; Leys David; Scrutton Nigel S
Mechanism of FAD reduction and role of active site residues His-225 and Tyr-259 in Arthrobacter globiformis dimethylglycine oxidase: analysis of mutant structure and catalytic function.
Biochemistry 2006;45(37):11151-61.
2006: Sutcliffe Michael J; Masgrau Laura; Roujeinikova Anna; Johannissen Linus O; Hothi Parvinder; Basran Jaswir; Ranaghan Kara E; Mulholland Adrian J; Leys David; Scrutton Nigel S
Hydrogen tunnelling in enzyme-catalysed H-transfer reactions: flavoprotein and quinoprotein systems.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2006;361(1472):1375-86.
2006: Jones Alex R; Scrutton Nigel S; Woodward Jonathan R
Magnetic field effects and radical pair mechanisms in enzymes: a reappraisal of the horseradish peroxidase system.
Journal of the American Chemical Society 2006;128(26):8408-9.
2006: Scrutton Nigel S; Sutcliffe Michael J; Leslie Dutton P
Quantum catalysis in enzymes: beyond the transition state theory paradigm. A Discussion Meeting held at the Royal Society on 14 and 15 November 2005.
Journal of the Royal Society, Interface / the Royal Society 2006;3(8):465-9.
2006: Combe Jonathan P; Basran Jaswir; Hothi Parvinder; Leys David; Rigby Stephen E J; Munro Andrew W; Scrutton Nigel S
Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis.
The Journal of biological chemistry 2006;281(26):17977-88.
2006: Masgrau Laura; Roujeinikova Anna; Johannissen Linus O; Hothi Parvinder; Basran Jaswir; Ranaghan Kara E; Mulholland Adrian J; Sutcliffe Michael J; Scrutton Nigel S; Leys David
Atomic description of an enzyme reaction dominated by proton tunneling.
Science (New York, N.Y.) 2006;312(5771):237-41.
2005: Polticelli Fabio; Basran Jaswir; Faso Carmen; Cona Alessandra; Minervini Giovanni; Angelini Riccardo; Federico Rodolfo; Scrutton Nigel S; Tavladoraki Paraskevi
Lys300 plays a major role in the catalytic mechanism of maize polyamine oxidase.
Biochemistry 2005;44(49):16108-20.
2005: Hothi Parvinder; Khadra Khalid Abu; Combe Jonathan P; Leys David; Scrutton Nigel S
Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis. Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans.
The FEBS journal 2005;272(22):5894-909.
2005: Neeli Rajasekhar; Girvan Hazel M; Lawrence Andrew; Warren Martin J; Leys David; Scrutton Nigel S; Munro Andrew W
The dimeric form of flavocytochrome P450 BM3 is catalytically functional as a fatty acid hydroxylase.
FEBS letters 2005;579(25):5582-8.
2005: Khan Huma; Barna Terez; Bruce Neil C; Munro Andrew W; Leys David; Scrutton Nigel S
Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase. Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181, His184.
The FEBS journal 2005;272(18):4660-71.
2005: Marshall Ker R; Gong Min; Wodke Leigh; Lamb John H; Jones Donald J L; Farmer Peter B; Scrutton Nigel S; Munro Andrew W
The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity.
The Journal of biological chemistry 2005;280(35):30735-40.
2005: Monaghan Phillip J; Leys David; Scrutton Nigel S
Crystallization and preliminary X-ray diffraction analysis of a flavoenzyme amine dehydrogenase/oxidase from Pyrococcus furiosus DSM 3638.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 8):756-8.
2005: McLean K J; Sabri M; Marshall K R; Lawson R J; Lewis D G; Clift D; Balding P R; Dunford A J; Warman A J; McVey J P; Quinn A-M; Sutcliffe M J; Scrutton N S; Munro A W
Biodiversity of cytochrome P450 redox systems.
Biochemical Society transactions 2005;33(Pt 4):796-801.
2005: Scrutton N S; Leys D
Crystal structure of DMGO provides a prototype for a new tetrahydrofolate-binding fold.
Biochemical Society transactions 2005;33(Pt 4):776-9.
2005: Rigby S E; Basran J; Combe J P; Mohsen A W; Toogood H; van Thiel A; Sutcliffe M J; Leys D; Munro A W; Scrutton N S
Flavoenzyme catalysed oxidation of amines: roles for flavin and protein-based radicals.
Biochemical Society transactions 2005;33(Pt 4):754-7.
2005: Warman A J; Roitel O; Neeli R; Girvan H M; Seward H E; Murray S A; McLean K J; Joyce M G; Toogood H; Holt R A; Leys D; Scrutton N S; Munro A W
Flavocytochrome P450 BM3: an update on structure and mechanism of a biotechnologically important enzyme.
Biochemical Society transactions 2005;33(Pt 4):747-53.
2005: Toogood Helen S; van Thiel Adam; Scrutton Nigel S; Leys David
Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein.
The Journal of biological chemistry 2005;280(34):30361-6.
2005: Messiha Hanan L; Bruce Neil C; Sattelle Benedict M; Sutcliffe Michael J; Munro Andrew W; Scrutton Nigel S
Role of active site residues and solvent in proton transfer and the modulation of flavin reduction potential in bacterial morphinone reductase.
The Journal of biological chemistry 2005;280(29):27103-10.
2005: Neeli Rajasekhar; Roitel Olivier; Scrutton Nigel S; Munro Andrew W
Switching pyridine nucleotide specificity in P450 BM3: mechanistic analysis of the W1046H and W1046A enzymes.
The Journal of biological chemistry 2005;280(18):17634-44.
2005: Hothi Parvinder; Sutcliffe Michael J; Scrutton Nigel S
Kinetic isotope effects and ligand binding in PQQ-dependent methanol dehydrogenase.
The Biochemical journal 2005;388(Pt 1):123-33.
2005: Messiha Hanan Latif; Munro Andrew W; Bruce Neil C; Barsukov Igor; Scrutton Nigel S
Reaction of morphinone reductase with 2-cyclohexen-1-one and 1-nitrocyclohexene: proton donation, ligand binding, and the role of residues Histidine 186 and Asparagine 189.
The Journal of biological chemistry 2005;280(11):10695-709.
2005: Rigby Stephen E J; Hynson Robert M G; Ramsay Rona R; Munro Andrew W; Scrutton Nigel S
A stable tyrosyl radical in monoamine oxidase A.
The Journal of biological chemistry 2005;280(6):4627-31.
2004: Leys David; Scrutton Nigel S
Electrical circuitry in biology: emerging principles from protein structure.
Current opinion in structural biology 2004;14(6):642-7.
2004: Scrutton Nigel S
Chemical aspects of amine oxidation by flavoprotein enzymes.
Natural product reports 2004;21(6):722-30.
2004: Masgrau Laura; Basran Jaswir; Hothi Parvinder; Sutcliffe Michael J; Scrutton Nigel S
Hydrogen tunneling in quinoproteins.
Archives of biochemistry and biophysics 2004;428(1):41-51.
2004: Toogood Helen S; van Thiel Adam; Basran Jaswir; Sutcliffe Mike J; Scrutton Nigel S; Leys David
Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex.
The Journal of biological chemistry 2004;279(31):32904-12.
2004: Khan Huma; Barna Terez; Harris Richard J; Bruce Neil C; Barsukov Igor; Munro Andrew W; Moody Peter C E; Scrutton Nigel S
Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation.
The Journal of biological chemistry 2004;279(29):30563-72.
2004: Williams Richard E; Rathbone Deborah A; Scrutton Nigel S; Bruce Neil C
Biotransformation of explosives by the old yellow enzyme family of flavoproteins.
Applied and environmental microbiology 2004;70(6):3566-74.
2004: Dunford Adrian J; Marshall Ker R; Munro Andrew W; Scrutton Nigel S
Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395.
European journal of biochemistry / FEBS 2004;271(12):2548-60.
2004: Mohsen Al-Walid A; Rigby Stephen E J; Jensen Kaj Frank; Munro Andrew W; Scrutton Nigel S
Thermodynamic basis of electron transfer in dihydroorotate dehydrogenase B from Lactococcus lactis: analysis by potentiometry, EPR spectroscopy, and ENDOR spectroscopy.
Biochemistry 2004;43(21):6498-510.
2004: Perry Ashlee; Tambyrajah Winston; Grossmann J Günter; Lian Lu-Yun; Scrutton Nigel S
Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by NMR spectroscopy and solution X-ray scattering and interactions with rubredoxin reductase.
Biochemistry 2004;43(11):3167-82.
2004: Olteanu Horatiu; Wolthers Kirsten R; Munro Andrew W; Scrutton Nigel S; Banerjee Ruma
Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase.
Biochemistry 2004;43(7):1988-97.
2004: Wolthers Kirsten R; Scrutton Nigel S
Electron transfer in human methionine synthase reductase studied by stopped-flow spectrophotometry.
Biochemistry 2004;43(2):490-500.
2004: Leys David; Basran Jaswir; Talfournier François; Chohan Kamaldeep K; Munro Andrew W; Sutcliffe Michael J; Scrutton Nigel S
Flavin radicals, conformational sampling and robust design principles in interprotein electron transfer: the trimethylamine dehydrogenase-electron-transferring flavoprotein complex.
Biochemical Society symposium 2004;(71):1-14.
2003: Basran Jaswir; Harris Richard J; Sutcliffe Michael J; Scrutton Nigel S
H-tunneling in the multiple H-transfers of the catalytic cycle of morphinone reductase and in the reductive half-reaction of the homologous pentaerythritol tetranitrate reductase.
The Journal of biological chemistry 2003;278(45):43973-82.
2003: Roitel Olivier; Scrutton Nigel S; Munro Andrew W
Electron transfer in flavocytochrome P450 BM3: kinetics of flavin reduction and oxidation, the role of cysteine 999, and relationships with mammalian cytochrome P450 reductase.
Biochemistry 2003;42(36):10809-21.
2003: Leys David; Basran Jaswir; Scrutton Nigel S
Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase.
The EMBO journal 2003;22(16):4038-48.
2003: Gutierrez Aldo; Munro Andrew W; Grunau Alex; Wolf C Roland; Scrutton Nigel S; Roberts Gordon C K
Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding. Relaxation kinetic studies with coenzyme analogues.
European journal of biochemistry / FEBS 2003;270(12):2612-21.
2003: Munro A W; McLean K J; Marshall K R; Warman A J; Lewis G; Roitel O; Sutcliffe M J; Kemp C A; Modi S; Scrutton N S; Leys D
Cytochromes P450: novel drug targets in the war against multidrug-resistant Mycobacterium tuberculosis.
Biochemical Society transactions 2003;31(Pt 3):625-30.
2003: Gutierrez A; Grunau A; Paine M; Munro A W; Wolf C R; Roberts G C K; Scrutton N S
Electron transfer in human cytochrome P450 reductase.
Biochemical Society transactions 2003;31(Pt 3):497-501.
2003: McLean Kirsty J; Scrutton Nigel S; Munro Andrew W
Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA.
The Biochemical journal 2003;372(Pt 2):317-27.
2003: Hothi Parvinder; Basran Jaswir; Sutcliffe Michael J; Scrutton Nigel S
Effects of multiple ligand binding on kinetic isotope effects in PQQ-dependent methanol dehydrogenase.
Biochemistry 2003;42(13):3966-78.
2003: Wolthers Kirsten R; Basran Jaswir; Munro Andrew W; Scrutton Nigel S
Molecular dissection of human methionine synthase reductase: determination of the flavin redox potentials in full-length enzyme and isolated flavin-binding domains.
Biochemistry 2003;42(13):3911-20.
2003: Finn Robert D; Basran Jaswir; Roitel Olivier; Wolf C Roland; Munro Andrew W; Paine Mark J I; Scrutton Nigel S
Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1.
European journal of biochemistry / FEBS 2003;270(6):1164-75.
2003: Leys David; Basran Jaswir; Talfournier François; Sutcliffe Michael J; Scrutton Nigel S
Extensive conformational sampling in a ternary electron transfer complex.
Nature structural biology 2003;10(3):219-25.
2003: Loechel Claudia; Basran Amrik; Basran Jaswir; Scrutton Nigel S; Hall Elizabeth A H
Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation.
The Analyst 2003;128(2):166-72.
2002: Knight Kirsty; Scrutton Nigel S
Stopped-flow kinetic studies of electron transfer in the reductase domain of neuronal nitric oxide synthase: re-evaluation of the kinetic mechanism reveals new enzyme intermediates and variation with cytochrome P450 reductase.
The Biochemical journal 2002;367(Pt 1):19-30.
2002: Barna Terez; Messiha Hanan Latif; Petosa Carlo; Bruce Neil C; Scrutton Nigel S; Moody Peter C E
Crystal structure of bacterial morphinone reductase and properties of the C191A mutant enzyme.
The Journal of biological chemistry 2002;277(34):30976-83.
2002: Lad Latesh; Mewies Martin; Basran Jaswir; Scrutton Nigel S; Raven Emma L
Role of histidine 42 in ascorbate peroxidase. Kinetic analysis of the H42A and H42E variants.
European journal of biochemistry / FEBS 2002;269(13):3182-92.
2002: Sutcliffe Michael J; Scrutton Nigel S
A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes.
European journal of biochemistry / FEBS 2002;269(13):3096-102.
2002: Khan Huma; Harris Richard J; Barna Terez; Craig Daniel H; Bruce Neil C; Munro Andrew W; Moody Peter C E; Scrutton Nigel S
Kinetic and structural basis of reactivity of pentaerythritol tetranitrate reductase with NADPH, 2-cyclohexenone, nitroesters, and nitroaromatic explosives.
The Journal of biological chemistry 2002;277(24):21906-12.
2002: Basran Jaswir; Bhanji Nina; Basran Amrik; Nietlispach Daniel; Mistry Sharad; Meskys Rolandas; Scrutton Nigel S
Mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of Arthrobacter globiformis studied by stopped-flow spectrophotometry.
Biochemistry 2002;41(14):4733-43.
2002: Gutierrez Aldo; Paine Mark; Wolf C Roland; Scrutton Nigel S; Roberts Gordon C K
Relaxation kinetics of cytochrome P450 reductase: internal electron transfer is limited by conformational change and regulated by coenzyme binding.
Biochemistry 2002;41(14):4626-37.
2002: Jones Matthew; Talfournier Francois; Bobrov Anton; Grossmann J Günter; Vekshin Nikolai; Sutcliffe Michael J; Scrutton Nigel S
Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.
The Journal of biological chemistry 2002;277(10):8457-65.
2001: Basran J; Sutcliffe M J; Scrutton N S
Optimizing the Michaelis complex of trimethylamine dehydrogenase: identification of interactions that perturb the ionization of substrate and facilitate catalysis with trimethylamine base.
The Journal of biological chemistry 2001;276(46):42887-92.
2001: Craig D H; Barna T; Moody P C; Bruce N C; Chapman S K; Munro A W; Scrutton N S
Effects of environment on flavin reactivity in morphinone reductase: analysis of enzymes displaying differential charge near the N-1 atom and C-2 carbonyl region of the active-site flavin.
The Biochemical journal 2001;359(Pt 2):315-23.
2001: Faulder P F; Tresadern G; Chohan K K; Scrutton N S; Sutcliffe M J; Hillier I H; Burton N A
QM/MM studies show substantial tunneling for the hydrogen-transfer reaction in methylamine dehydrogenase.
Journal of the American Chemical Society 2001;123(35):8604-5.
2001: Chohan K K; Jones M; Grossmann J G; Frerman F E; Scrutton N S; Sutcliffe M J
Protein dynamics enhance electronic coupling in electron transfer complexes.
The Journal of biological chemistry 2001;276(36):34142-7.
2001: Stevenson T H; Gutierrez A F; Alderton W K; Lian L; Scrutton N S
Kinetics of CO binding to the haem domain of murine inducible nitric oxide synthase: differential effects of haem domain ligands.
The Biochemical journal 2001;358(Pt 1):201-8.
2001: Barna T M; Khan H; Bruce N C; Barsukov I; Scrutton N S; Moody P C
Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme.
Journal of molecular biology 2001;310(2):433-47.
2001: Basran J; Sutcliffe M J; Scrutton N S
Deuterium isotope effects during carbon-hydrogen bond cleavage by trimethylamine dehydrogenase. Implications for mechanism and vibrationally assisted hydrogen tunneling in wild-type and mutant enzymes.
The Journal of biological chemistry 2001;276(27):24581-7.
2001: Meskys R; Harris R J; Casaite V; Basran J; Scrutton N S
Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp.: implications for glycine betaine catabolism.
European journal of biochemistry / FEBS 2001;268(12):3390-8.
2001: Talfournier F; Munro A W; Basran J; Sutcliffe M J; Daff S; Chapman S K; Scrutton N S
alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone.
The Journal of biological chemistry 2001;276(23):20190-6.
2001: Basran J; Patel S; Sutcliffe M J; Scrutton N S
Importance of barrier shape in enzyme-catalyzed reactions. Vibrationally assisted hydrogen tunneling in tryptophan tryptophylquinone-dependent amine dehydrogenases.
The Journal of biological chemistry 2001;276(9):6234-42.
2001: Gutierrez A; Lian L Y; Wolf C R; Scrutton N S; Roberts G C
Stopped-flow kinetic studies of flavin reduction in human cytochrome P450 reductase and its component domains.
Biochemistry 2001;40(7):1964-75.
2001: Perry A; Lian L Y; Scrutton N S
Two-iron rubredoxin of Pseudomonas oleovorans: production, stability and characterization of the individual iron-binding domains by optical, CD and NMR spectroscopies.
The Biochemical journal 2001;354(Pt 1):89-98.
2001: Williams R E; Rathbone D A; Moody P C; Scrutton N S; Bruce N C
Degradation of explosives by nitrate ester reductases.
Biochemical Society symposium 2001;(68):143-53.
2000: Gutierrez A; Doehr O; Paine M; Wolf C R; Scrutton N S; Roberts G C
Trp-676 facilitates nicotinamide coenzyme exchange in the reductive half-reaction of human cytochrome P450 reductase: properties of the soluble W676H and W676A mutant reductases.
Biochemistry 2000;39(51):15990-9.
2000: Basran J; Chohan K K; Sutcliffe M J; Scrutton N S
Differential coupling through Val-344 and Tyr-442 of trimethylamine dehydrogenase in electron transfer reactions with ferricenium ions and electron transferring flavoprotein.
Biochemistry 2000;39(31):9188-200.
2000: Harris R J; Meskys R; Sutcliffe M J; Scrutton N S
Kinetic studies of the mechanism of carbon-hydrogen bond breakage by the heterotetrameric sarcosine oxidase of Arthrobacter sp. 1-IN.
Biochemistry 2000;39(6):1189-98.
2000: Scrutton N S; Sutcliffe M J
Trimethylamine dehydrogenase and electron transferring flavoprotein.
Sub-cellular biochemistry 2000;35():145-81.
1999: Scrutton N S
Colworth Medal Lecture. Enzymes in the quantum world.
Biochemical Society transactions 1999;27(6):767-79.
1999: Roberts P; Basran J; Wilson E K; Hille R; Scrutton N S
Redox cycles in trimethylamine dehydrogenase and mechanism of substrate inhibition.
Biochemistry 1999;38(45):14927-40.
1999: Scrutton N S; Basran J; Sutcliffe M J
New insights into enzyme catalysis. Ground state tunnelling driven by protein dynamics.
European journal of biochemistry / FEBS 1999;264(3):666-71.
1999: Basran J; Sutcliffe M J; Hille R; Scrutton N S
Reductive half-reaction of the H172Q mutant of trimethylamine dehydrogenase: evidence against a carbanion mechanism and assignment of kinetically influential ionizations in the enzyme-substrate complex.
The Biochemical journal 1999;341 ( Pt 2)():307-14.
1999: Basran J; Jang M H; Sutcliffe M J; Hille R; Scrutton N S
The role of Tyr-169 of trimethylamine dehydrogenase in substrate oxidation and magnetic interaction between FMN cofactor and the 4Fe/4S center.
The Journal of biological chemistry 1999;274(19):13155-61.
1999: Basran J; Sutcliffe M J; Scrutton N S
Enzymatic H-transfer requires vibration-driven extreme tunneling.
Biochemistry 1999;38(10):3218-22.
1999: Scrutton N S; Basran J; Wilson E K; Chohan K K; Jang M H; Sutcliffe M J; Hille R
Electron transfer in trimethylamine dehydrogenase and electron-transferring flavoprotein.
Biochemical Society transactions 1999;27(2):196-201.
1999: Scrutton N S
Identification of covalent flavoproteins and analysis of the covalent link.
Methods in molecular biology (Clifton, N.J.) 1999;131():181-93.
1998: Lee H J; Basran J; Scrutton N S
Electron transfer from flavin to iron in the Pseudomonas oleovorans rubredoxin reductase-rubredoxin electron transfer complex.
Biochemistry 1998;37(44):15513-22.
1998: Moody P C; Shikotra N; French C E; Bruce N C; Scrutton N S
Crystallization and preliminary diffraction studies of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.
Acta crystallographica. Section D, Biological crystallography 1998;54(Pt 4):675-7.
1998: Ertughrul O W; Errington N; Raza S; Sutcliffe M J; Rowe A J; Scrutton N S
Probing the stabilizing role of C-terminal residues in trimethylamine dehydrogenase.
Protein engineering 1998;11(6):447-55.
1998: Craig D H; Moody P C; Bruce N C; Scrutton N S
Reductive and oxidative half-reactions of morphinone reductase from Pseudomonas putida M10: a kinetic and thermodynamic analysis.
Biochemistry 1998;37(20):7598-607.
1998: Mewies M; McIntire W S; Scrutton N S
Covalent attachment of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) to enzymes: the current state of affairs.
Protein science : a publication of the Protein Society 1998;7(1):7-20.
1997: Lee H J; Lian L Y; Scrutton N S
Recombinant two-iron rubredoxin of Pseudomonas oleovorans: overexpression, purification and characterization by optical, CD and 113Cd NMR spectroscopies.
The Biochemical journal 1997;328 ( Pt 1)():131-6.
1997: Moody P C; Shikotra N; French C E; Bruce N C; Scrutton N S
Crystallization and preliminary diffraction studies of morphinone reductase, a flavoprotein involved in the degradation of morphine alkaloids.
Acta crystallographica. Section D, Biological crystallography 1997;53(Pt 5):619-21.
1997: Mewies M; Basran J; Packman L C; Hille R; Scrutton N S
Involvement of a flavin iminoquinone methide in the formation of 6-hydroxyflavin mononucleotide in trimethylamine dehydrogenase: a rationale for the existence of 8alpha-methyl and C6-linked covalent flavoproteins.
Biochemistry 1997;36(23):7162-8.
1997: Basran J; Mewies M; Mathews F S; Scrutton N S
Selective modification of alkylammonium ion specificity in trimethylamine dehydrogenase by the rational engineering of cation-pi bonding.
Biochemistry 1997;36(8):1989-98.
1997: Wilson E K; Scrutton N S; Cölfen H; Harding S E; Jacobsen M P; Winzor D J
An ultracentrifugal approach to quantitative characterization of the molecular assembly of a physiological electron-transfer complex: the interaction of electron-transferring flavoprotein with trimethylamine dehydrogenase.
European journal of biochemistry / FEBS 1997;243(1-2):393-9.
1997: Wilson E K; Huang L; Sutcliffe M J; Mathews F S; Hille R; Scrutton N S
An exposed tyrosine on the surface of trimethylamine dehydrogenase facilitates electron transfer to electron transferring flavoprotein: kinetics of transfer in wild-type and mutant complexes.
Biochemistry 1997;36(1):41-8.
1996: Scrutton N S; Raine A R
Cation-pi bonding and amino-aromatic interactions in the biomolecular recognition of substituted ammonium ligands.
The Biochemical journal 1996;319 ( Pt 1)():1-8.
1996: Wilson E K; Huang L; Hille R; Mathews F S; Scrutton N S
Electron transfer in trimethylamine dehydrogenase: directed mutagenesis of a potential tunneling pathway.
Biochemical Society transactions 1996;24(3):456S.
1996: Lee H J; Lian L Y; Scrutton N S
Rubredoxin/rubredoxin reductase of Pseudomonas oleovorans: a model system for investigating interprotein electron transfer.
Biochemical Society transactions 1996;24(3):447S.
1996: Mewies M; Packman L C; Mathews F S; Scrutton N S
Flavinylation in wild-type trimethylamine dehydrogenase and differentially charged mutant enzymes: a study of the protein environment around the N1 of the flavin isoalloxazine.
The Biochemical journal 1996;317 ( Pt 1)():267-72.
1996: Huang L; Scrutton N S; Hille R
Reaction of the C30A mutant of trimethylamine dehydrogenase with diethylmethylamine.
The Journal of biological chemistry 1996;271(23):13401-6.
1995: Raine A R; Yang C C; Packman L C; White S A; Mathews F S; Scrutton N S
Protein recognition of ammonium cations using side-chain aromatics: a structural variation for secondary ammonium ligands.
Protein science : a publication of the Protein Society 1995;4(12):2625-8.
1995: Bashir A; Perham R N; Scrutton N S; Berry A
Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase.
The Biochemical journal 1995;312 ( Pt 2)():527-33.
1995: Mewies M; Packman L C; Scrutton N S
Dissection of the FMN-binding site in trimethylamine dehydrogenase.
Biochemical Society transactions 1995;23(4):509S.
1995: Yang C C; Packman L C; Scrutton N S
The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition.
European journal of biochemistry / FEBS 1995;232(1):264-71.
1995: Packman L C; Mewies M; Scrutton N S
The flavinylation reaction of trimethylamine dehydrogenase. Analysis by directed mutagenesis and electrospray mass spectrometry.
The Journal of biological chemistry 1995;270(22):13186-91.
1995: Wilson E K; Mathews F S; Packman L C; Scrutton N S
Electron tunneling in substrate-reduced trimethylamine dehydrogenase: kinetics of electron transfer and analysis of the tunneling pathway.
Biochemistry 1995;34(8):2584-91.
1994: Rietveld P; Arscott L D; Berry A; Scrutton N S; Deonarain M P; Perham R N; Williams C H
Reductive and oxidative half-reactions of glutathione reductase from Escherichia coli.
Biochemistry 1994;33(46):13888-95.
1994: Raine A R; Scrutton N S; Mathews F S
On the evolution of alternate core packing in eightfold beta/alpha-barrels.
Protein science : a publication of the Protein Society 1994;3(10):1889-92.
1994: Scrutton N S; Packman L C; Mathews F S; Rohlfs R J; Hille R
Assembly of redox centers in the trimethylamine dehydrogenase of bacterium W3A1. Properties of the wild-type enzyme and a C30A mutant expressed from a cloned gene in Escherichia coli.
The Journal of biological chemistry 1994;269(19):13942-50.
1994: Scrutton N S
alpha/beta barrel evolution and the modular assembly of enzymes: emerging trends in the flavin oxidase/dehydrogenase family.
BioEssays : news and reviews in molecular, cellular and developmental biology 1994;16(2):115-22.
1993: Bocanegra J A; Scrutton N S; Perham R N
Creation of an NADP-dependent pyruvate dehydrogenase multienzyme complex by protein engineering.
Biochemistry 1993;32(11):2737-40.
1992: Scrutton N S; Deonarain M P; Berry A; Perham R N
Cooperativity induced by a single mutation at the subunit interface of a dimeric enzyme: glutathione reductase.
Science (New York, N.Y.) 1992;258(5085):1140-3.
1992: Boyd G; Mathews F S; Packman L C; Scrutton N S
Trimethylamine dehydrogenase of bacterium W3A1. Molecular cloning, sequence determination and over-expression of the gene.
FEBS letters 1992;308(3):271-6.
1992: Deonarain M P; Scrutton N S; Perham R N
Engineering surface charge. 2. A method for purifying heterodimers of Escherichia coli glutathione reductase.
Biochemistry 1992;31(5):1498-504.
1992: Deonarain M P; Scrutton N S; Perham R N
Engineering surface charge. 1. A method for detecting subunit exchange in Escherichia coli glutathione reductase.
Biochemistry 1992;31(5):1491-7.
1991: Perham R N; Scrutton N S; Berry A
New enzymes for old: redesigning the coenzyme and substrate specificities of glutathione reductase.
BioEssays : news and reviews in molecular, cellular and developmental biology 1991;13(10):515-25.
1990: Scrutton N S; Berry A; Deonarain M P; Perham R N
Active site complementation in engineered heterodimers of Escherichia coli glutathione reductase created in vivo.
Proceedings. Biological sciences / The Royal Society 1990;242(1305):217-24.
1990: Deonarain M P; Scrutton N S; Berry A; Perham R N
Directed mutagenesis of the redox-active disulphide bridge in glutathione reductase from Escherichia coli.
Proceedings. Biological sciences / The Royal Society 1990;241(1302):179-86.
1990: Scrutton N S; Berry A; Perham R N
Redesign of the coenzyme specificity of a dehydrogenase by protein engineering.
Nature 1990;343(6253):38-43.
1989: Deonarain M P; Berry A; Scrutton N S; Perham R N
Alternative proton donors/acceptors in the catalytic mechanism of the glutathione reductase of Escherichia coli: the role of histidine-439 and tyrosine-99.
Biochemistry 1989;28(25):9602-7.
1989: Berry A; Scrutton N S; Perham R N
Switching kinetic mechanism and putative proton donor by directed mutagenesis of glutathione reductase.
Biochemistry 1989;28(3):1264-9.
1988: Scrutton N S; Berry A; Perham R N
Engineering of an intersubunit disulphide bridge in glutathione reductase from Escherichia coli.
FEBS letters 1988;241(1-2):46-50.
1988: Perham R N; Berry A; Scrutton N S
Flavoprotein disulphide oxidoreductases: protein engineering of glutathione reductase from Escherichia coli.
Biochemical Society transactions 1988;16(2):84-7.
1987: Scrutton N S; Berry A; Perham R N
Purification and characterization of glutathione reductase encoded by a cloned and over-expressed gene in Escherichia coli.
The Biochemical journal 1987;245(3):875-80.

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