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Junichi Sekiguchi

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Kohichi Kaneda; Kaori Ohishi; Junichi Sekiguchi; Toshio Shida
Characterization of the AP endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of two important tryptophan residues to AP site recognition.
Tatsuya Fukushima; Anahita Afkham; Shin-Ichirou Kurosawa; Taichi Tanabe; Hiroki Yamamoto; Junichi Sekiguchi
A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis.
Kohichi Kaneda; Junichi Sekiguchi; Toshio Shida
Role of the tryptophan residue in the vicinity of the catalytic center of exonuclease III family AP endonucleases: AP site recognition mechanism.

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2006: Kohichi Kaneda; Kaori Ohishi; Junichi Sekiguchi; Toshio Shida
Characterization of the AP endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of two important tryptophan residues to AP site recognition.
Bioscience, biotechnology, and biochemistry 2006;70(9):2213-21.
2006: Tatsuya Fukushima; Anahita Afkham; Shin-Ichirou Kurosawa; Taichi Tanabe; Hiroki Yamamoto; Junichi Sekiguchi
A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis.
Journal of bacteriology 2006;188(15):5541-50.
2006: Kohichi Kaneda; Junichi Sekiguchi; Toshio Shida
Role of the tryptophan residue in the vicinity of the catalytic center of exonuclease III family AP endonucleases: AP site recognition mechanism.
Nucleic acids research 2006;34(5):1552-63.
2005: Masakuni Serizawa; Keisuke Kodama; Hiroki Yamamoto; Kazuo Kobayashi; Naotake Ogasawara; Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Bioscience, biotechnology, and biochemistry 2005;69(11):2155-69.
2005: Masakuni Serizawa; Junichi Sekiguchi
The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily.
Microbiology (Reading, England) 2005;151(Pt 6):1769-78.
2005: Tatsuya Fukushima; Toshihiko Kitajima; Junichi Sekiguchi
A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro.
Journal of bacteriology 2005;187(4):1287-92.
2004: Tatsuya Fukushima; Taichi Tanabe; Hiroki Yamamoto; Shigeo Hosoya; Tsutomu Sato; Hirofumi Yoshikawa; Junichi Sekiguchi
Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
Journal of biochemistry 2004;136(3):283-91.
2004: Masakuni Serizawa; Hiroki Yamamoto; Hirotake Yamaguchi; Yasutaro Fujita; Kazuo Kobayashi; Naotake Ogasawara; Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene 2004;329():125-36.
2004: Hiroyuki Yamaguchi; Kazumi Furuhata; Tatsuya Fukushima; Hiroki Yamamoto; Junichi Sekiguchi
Characterization of a new Bacillus subtilis peptidoglycan hydrolase gene, yvcE (named cwlO), and the enzymatic properties of its encoded protein.
Journal of bioscience and bioengineering 2004;98(3):174-81.
2003: Hiroki Yamamoto; Shin-ichirou Kurosawa; Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
Journal of bacteriology 2003;185(22):6666-77.
2003: Tatsuya Fukushima; Shu Ishikawa; Hiroki Yamamoto; Naotake Ogasawara; Junichi Sekiguchi
Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis.
Journal of biochemistry 2003;133(4):475-83.
2002: Tatsuya Fukushima; Hiroki Yamamoto; Abdelmadjid Atrih; Simon J Foster; Junichi Sekiguchi
A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
Journal of bacteriology 2002;184(21):6007-15.
2002: Toshio Shida; Kohsuke Mukaijo; Shu Ishikawa; Hiroki Yamamoto; Junichi Sekiguchi
Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH.
Bioscience, biotechnology, and biochemistry 2002;66(7):1555-8.
2002: Gota Kobayashi; Kaori Fujii; Masakuni Serizawa; Hiroki Yamamoto; Junichi Sekiguchi
Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis.
Journal of bioscience and bioengineering 2002;93(1):15-9.
2001: Hiroki Yamamoto; Masakuni Serizawa; J Thompson; Junichi Sekiguchi
Regulation of the glv operon in Bacillus subtilis: YfiA (GlvR) is a positive regulator of the operon that is repressed through CcpA and cre.
Journal of bacteriology 2001;183(17):5110-21.
2001: Toshio Shida; H Hattori; F Ise; Junichi Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
The Journal of biological chemistry 2001;276(30):28140-6.
2000: J Toida; M Fukuzawa; Gota Kobayashi; K Ito; Junichi Sekiguchi
Cloning and sequencing of the triacylglycerol lipase gene of Aspergillus oryzae and its expression in Escherichia coli.
FEMS microbiology letters 2000;189(2):159-64.
2000: H Yamamoto; M Murata; Junichi Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Molecular microbiology 2000;37(4):898-912.
2000: Gota Kobayashi; J Toida; T Akamatsu; Hiroki Yamamoto; T Shida; Junichi Sekiguchi
Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants.
FEMS microbiology letters 2000;188(2):165-9.
2000: Toshio Shida; H Hattori; F Ise; Junichi Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Bioscience, biotechnology, and biochemistry 2000;64(7):1522-5.
2000: Gota Kobayashi; J Toida; T Akamatsu; Hiroki Yamamoto; T Shida; Junichi Sekiguchi
Accumulation of a recombinant Aspergillus oryzae lipase artificially localized on the Bacillus subtilis cell surface.
Journal of bioscience and bioengineering 2000;90(4):422-5.
1999: Shu Ishikawa; S Kawahara; Junichi Sekiguchi
Cloning and expression of two autolysin genes, cwIU and cwIV, which are tandemly arranged on the chromosome of Bacillus polymyxa var. colistinus.
Molecular & general genetics : MGG 1999;262(4-5):738-48.
1999: FA Nugroho; Hiroki Yamamoto; Y Kobayashi; Junichi Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
Journal of bacteriology 1999;181(20):6230-7.
1999: Toshio Shida; T Ogawa; Naotake Ogasawara; Junichi Sekiguchi
Characterization of Bacillus subtilis ExoA protein: a multifunctional DNA-repair enzyme similar to the Escherichia coli exonuclease III.
Bioscience, biotechnology, and biochemistry 1999;63(9):1528-34.
1999: Junichi Sekiguchi; T Sato; Hideaki Nanamiya; Y Ohashi; Fujio Kawamura; H Takamatsu; T Kodama; K Watabe; Shu Ishikawa
[Formation, structure and germination of the spore of Bacillus subtilis].
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 1999;44(10):1460-6.
1999: Hiroki Yamamoto; M Mori; Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading, England) 1999;145 ( Pt 8)():2171-80.
1999: R Ohnishi; Shu Ishikawa; Junichi Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
Journal of bacteriology 1999;181(10):3178-84.
1998: Shu Ishikawa; Y Hara; R Ohnishi; Junichi Sekiguchi
Regulation of a new cell wall hydrolase gene, cwlF, which affects cell separation in Bacillus subtilis.
Journal of bacteriology 1998;180(9):2549-55.
1998: J Toida; Yukihiko Arikawa; K Kondou; M Fukuzawa; Junichi Sekiguchi
Purification and characterization of triacylglycerol lipase from Aspergillus oryzae.
Bioscience, biotechnology, and biochemistry 1998;62(4):759-63.
1998: Shu Ishikawa; K Yamane; Junichi Sekiguchi
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores.
Journal of bacteriology 1998;180(6):1375-80.
1998: T Shida; M Suda; Junichi Sekiguchi
Characterization of telomeric oligonucleotides: fidelity of repetitive nucleotide sequences.
Nucleosides & nucleotides 1998;17(1-3):575-84.
1997: Hiroki Yamamoto; S Uchiyama; FA Nugroho; Junichi Sekiguchi
Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein.
Gene 1997;194(2):191-9.
1997: K Yamanaka; J Araki; M Takano; Junichi Sekiguchi
Characterization of Bacillus subtilis mutants resistant to cold shock-induced autolysis.
FEMS microbiology letters 1997;150(2):269-75.
1997: Hiroki Yamamoto; S Uchiyama; FA Nugroho; Junichi Sekiguchi
A 23.4 kb segment at the 69 degrees-70 degrees region of the Bacillus subtilis genome.
Microbiology (Reading, England) 1997;143 ( Pt 4)():1317-20.
1997: T Shida; M Suda; Junichi Sekiguchi
Quadruplex formation of oligonucleotides containing G clusters and their nuclease resistance.
Nucleic acids symposium series 1997;(37):129-30.
1996: Hiroki Yamamoto; S Uchiyama; Junichi Sekiguchi
The Bacillus subtilis chromosome region near 78 degrees contains the genes encoding a new two-component system, three ABC transporters and a lipase.
Gene 1996;181(1-2):147-51.
1996: T Shida; M Noda; Junichi Sekiguchi
Cleavage of single- and double-stranded DNAs containing an abasic residue by Escherichia coli exonuclease III (AP endonuclease VI).
Nucleic acids research 1996;24(22):4572-6.
1996: Hiroki Yamamoto; S Uchiyama; Junichi Sekiguchi
Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization.
Microbiology (Reading, England) 1996;142 ( Pt 11)():3057-65.
1996: M H Rashid; Junichi Sekiguchi
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.
Journal of bacteriology 1996;178(22):6640-3.
1996: A Tsuchiya; H Nakazawa; J Toida; K Ohnishi; Junichi Sekiguchi
Cloning and nucleotide sequence of the mono- and diacylglycerol lipase gene (mdlB) of Aspergillus oryzae.
FEMS microbiology letters 1996;143(1):63-7.
1996: H Yamamoto; S Uchiyama; Junichi Sekiguchi
Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degrees-81 degrees region of the Bacillus subtilis genome containing the sspE locus.
DNA research : an international journal for rapid publication of reports on genes and genomes 1996;3(4):257-62.
1996: M H Rashid; A Tamakoshi; Junichi Sekiguchi
Effects of mecA and mecB (clpC) mutations on expression of sigD, which encodes an alternative sigma factor, and autolysin operons and on flagellin synthesis in Bacillus subtilis.
Journal of bacteriology 1996;178(16):4861-9.
1996: Hiroki Yamamoto; S Uchiyama; A N Fajar; N Ogasawara; Junichi Sekiguchi
Determination of a 12 kb nucleotide sequence around the 76 degrees region of the Bacillus subtilis chromosome.
Microbiology (Reading, England) 1996;142 ( Pt 6)():1417-21.
1995: M H Rashid; M Mori; Junichi Sekiguchi
Glucosaminidase of Bacillus subtilis: cloning, regulation, primary structure and biochemical characterization.
Microbiology (Reading, England) 1995;141 ( Pt 10)():2391-404.
1995: Junichi Sekiguchi; K Akeo; Hiroki Yamamoto; F K Khasanov; J C Alonso; A Kuroda
Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis. .
Journal of bacteriology 1995;177(19):5582-9.
1995: J Toida; K Kondoh; M Fukuzawa; K Ohnishi; Junichi Sekiguchi
Purification and characterization of a lipase from Aspergillus oryzae.
Bioscience, biotechnology, and biochemistry 1995;59(7):1199-203.
1995: H Yamamoto; A Naruse; T Ohsaki; Junichi Sekiguchi
Nucleotide sequence and characterization of the large mitochondrial rRNA gene of Penicillium urticae, and its comparison with those of other filamentous fungi.
Journal of biochemistry 1995;117(4):888-96.
1995: K Ohnishi; J Toida; H Nakazawa; Junichi Sekiguchi
Genome structure and nucleotide sequence of a lipolytic enzyme gene of Aspergillus oryzae.
FEMS microbiology letters 1995;126(2):145-50.
1995: T Shida; M Noda; Junichi Sekiguchi
The recognition of DNA containing an AP site by E.coli endonuclease VI (exonuclease III).
Nucleic acids symposium series 1995;(34):87-8.
1994: T Tokunaga; M H Rashid; A Kuroda; Junichi Sekiguchi
Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis.
Journal of bacteriology 1994;176(16):5177-80.
1993: Y Oda; R Nakayama; A Kuroda; Junichi Sekiguchi
Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis.
Molecular & general genetics : MGG 1993;241(3-4):380-8.
1993: A Kuroda; Y Asami; Junichi Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
Journal of bacteriology 1993;175(19):6260-8.
1993: M H Rashid; A Kuroda; Junichi Sekiguchi
Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility.
FEMS microbiology letters 1993;112(2):135-40.
1993: A Naruse; H Yamamoto; Junichi Sekiguchi
Nucleotide sequence of the large mitochondrial rRNA gene of Penicillium chrysogenum.
Biochimica et biophysica acta 1993;1172(3):353-6.
1993: A Kuroda; Junichi Sekiguchi
High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.
Journal of bacteriology 1993;175(3):795-801.
1992: A Kuroda; Junichi Sekiguchi
Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases.
FEMS microbiology letters 1992;74(1):109-13.
1992: A Kuroda; Y Sugimoto; T Funahashi; Junichi Sekiguchi
Genetic structure, isolation and characterization of a Bacillus licheniformis cell wall hydrolase.
Molecular & general genetics : MGG 1992;234(1):129-37.
1992: A Kuroda; M H Rashid; Junichi Sekiguchi
Molecular cloning and sequencing of the upstream region of the major Bacillus subtilis autolysin gene: a modifier protein exhibiting sequence homology to the major autolysin and the spoIID product.
Journal of general microbiology 1992;138(6):1067-76.
1992: T Shida; N Ikeda; Junichi Sekiguchi
Characterization of four-stranded DNA and RNA fragments.
Nucleic acids symposium series 1992;(27):53-4.
1991: N Kiuchi; A Naruse; H Yamamoto; Junichi Sekiguchi
Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene.
Agricultural and biological chemistry 1991;55(12):3053-7.
1991: A Kuroda; Junichi Sekiguchi
Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.
Journal of bacteriology 1991;173(22):7304-12.
1991: A Kuroda; M Imazeki; Junichi Sekiguchi
Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
FEMS microbiology letters 1991;65(1):9-13.
1991: T Shida; H Iwaori; Junichi Sekiguchi
Synthesis and properties of oligodeoxyribonucleotides containing abasic site.
Nucleic acids symposium series 1991;(25):97-8.
1990: A Kuroda; Junichi Sekiguchi
Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene.
Journal of general microbiology 1990;136(11):2209-16.
1990: Junichi Sekiguchi; H Ohsu; A Kuroda; H Moriyama; T Akamatsu
Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation.
Journal of general microbiology 1990;136(7):1223-30.
1990: Junichi Sekiguchi; T Ohsaki; H Yamamoto; K Koichi; T Shida
Characterization and comparison of mitochondrial DNAs and rRNAs from Penicillium urticae and P. chrysogenum.
Journal of general microbiology 1990;136(3):535-43.
1990: T Shida; N Ikeda; Junichi Sekiguchi
Interactions between quadruple-stranded oligodeoxyribonucleotides and drugs.
Nucleic acids symposium series 1990;(22):79-80.
1988: Junichi Sekiguchi; B Ezaki; K Kodama; T Akamatsu
Molecular cloning of a gene affecting the autolysin level and flagellation in Bacillus subtilis.
Journal of general microbiology 1988;134(6):1611-21.
1987: Junichi Sekiguchi; S Katayama; Y Yamada
6-Methyl-1,2,4-benzenetriol, a new intermediate in penicillic acid biosynthesis in Penicillium cyclopium.
Applied and environmental microbiology 1987;53(7):1531-5.