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Konstantin Severinov

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Co-Publications
Name
20
Darst, Seth
18
Kuznedelov, Konstantin
18
Minakhin, Leonid
15
Nikiforov, Vadim
15
Goldfarb, Alex
13
Mustaev, Arkady
11
Severinova, Elena
11
Phadtare, Sangita
11
Zenkin, Nikolay
10
Heyduk, Tomasz
9
Bass, Irina
9
Naryshkina, Tatyana
9
Wigneshweraraj, Siva
8
Buck, Martin
8
Kashlev, Mikhail

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Publications

TOP 3
Socias Sergio B; Severinov Konstantin; Salomon Raul A
The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA.
Phadtare Sangita; Severinov Konstantin
Comparative analysis of changes in gene expression due to RNA melting activities of translation initiation factor IF1 and a cold shock protein of the CspA family.
Van de Vijver Pieter; Vondenhoff Gaston H M; Kazakov Teymur S; Semenova Ekaterina; Kuznedelov Konstantin; Metlitskaya Anastasia; Van Aerschot Arthur; Severinov Konstantin
Synthetic microcin C analogs targeting different aminoacyl-tRNA synthetases.

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All Publications

2009: Socias Sergio B; Severinov Konstantin; Salomon Raul A
The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA.
FEMS microbiology letters 2009;301(1):124-9.
2009: Phadtare Sangita; Severinov Konstantin
Comparative analysis of changes in gene expression due to RNA melting activities of translation initiation factor IF1 and a cold shock protein of the CspA family.
Genes to cells : devoted to molecular & cellular mechanisms 2009;14(11):1227-39.
2009: Van de Vijver Pieter; Vondenhoff Gaston H M; Kazakov Teymur S; Semenova Ekaterina; Kuznedelov Konstantin; Metlitskaya Anastasia; Van Aerschot Arthur; Severinov Konstantin
Synthetic microcin C analogs targeting different aminoacyl-tRNA synthetases.
Journal of bacteriology 2009;191(20):6273-80.
2009: Pomares María Fernanda; Salomón Raúl A; Pavlova Olga; Severinov Konstantin; Farías Ricardo; Vincent Paula A
Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation.
Applied and environmental microbiology 2009;75(17):5734-8.
2009: Protsenko Alexey; Zakharova Marina; Nagornykh Maxim; Solonin Alexander; Severinov Konstantin
Transcription regulation of restriction-modification system Ecl18kI.
Nucleic acids research 2009;37(16):5322-30.
2009: Semenova Ekaterina; Nagornykh Maxim; Pyatnitskiy Mikhail; Artamonova Irena I; Severinov Konstantin
Analysis of CRISPR system function in plant pathogen Xanthomonas oryzae.
FEMS microbiology letters 2009;296(1):110-6.
2009: Bogdanova Ekaterina; Zakharova Marina; Streeter Simon; Taylor James; Heyduk Tomasz; Kneale Geoff; Severinov Konstantin
Transcription regulation of restriction-modification system Esp1396I.
Nucleic acids research 2009;37(10):3354-66.
2009: Metlitskaya Anastasia; Kazakov Teymur; Vondenhoff Gaston H; Novikova Maria; Shashkov Alexander; Zatsepin Timofei; Semenova Ekaterina; Zaitseva Natalia; Ramensky Vasily; Van Aerschot Arthur; Severinov Konstantin
Maturation of the translation inhibitor microcin C.
Journal of bacteriology 2009;191(7):2380-7.
2009: Sorokin Valeriy; Severinov Konstantin; Gelfand Mikhail S
Systematic prediction of control proteins and their DNA binding sites.
Nucleic acids research 2009;37(2):441-51.
2008: Lamour Valerie; Rutherford Steven T; Kuznedelov Konstantin; Ramagopal Udupi A; Gourse Richard L; Severinov Konstantin; Darst Seth A
Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein.
Journal of molecular biology 2008;383(2):367-79.
2008: Nechaev Sergei; Severinov Konstantin
RapA: completing the transcription cycle?
Structure (London, England : 1993) 2008;16(9):1294-5.
2008: Pavlova Olga; Mukhopadhyay Jayanta; Sineva Elena; Ebright Richard H; Severinov Konstantin
Systematic structure-activity analysis of microcin J25.
The Journal of biological chemistry 2008;283(37):25589-95.
2008: Barinova Nataliya; Kuznedelov Konstantin; Severinov Konstantin; Kulbachinskiy Andrey
Structural modules of RNA polymerase required for transcription from promoters containing downstream basal promoter element GGGA.
The Journal of biological chemistry 2008;283(33):22482-9.
2008: Wigneshweraraj Sivaramesh; Bose Daniel; Burrows Patricia C; Joly Nicolas; Schumacher Jörg; Rappas Mathieu; Pape Tillmann; Zhang Xiaodong; Stockley Peter; Severinov Konstantin; Buck Martin
Modus operandi of the bacterial RNA polymerase containing the sigma54 promoter-specificity factor.
Molecular microbiology 2008;68(3):538-46.
2008: Nechaev Sergei; Severinov Konstantin
The elusive object of desire--interactions of bacteriophages and their hosts.
Current opinion in microbiology 2008;11(2):186-93.
2008: Minakhin Leonid; Goel Manisha; Berdygulova Zhanna; Ramanculov Erlan; Florens Laurence; Glazko Galina; Karamychev Valeri N; Slesarev Alexei I; Kozyavkin Sergei A; Khromov Igor; Ackermann Hans-W; Washburn Michael; Mushegian Arcady; Severinov Konstantin
Genome comparison and proteomic characterization of Thermus thermophilus bacteriophages P23-45 and P74-26: siphoviruses with triplex-forming sequences and the longest known tails.
Journal of molecular biology 2008;378(2):468-80.
2008: Kazakov Teymur; Vondenhoff Gaston H; Datsenko Kirill A; Novikova Maria; Metlitskaya Anastasia; Wanner Barry L; Severinov Konstantin
Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
Journal of bacteriology 2008;190(7):2607-10.
2008: Savalia Dhruti; Westblade Lars F; Goel Manisha; Florens Laurence; Kemp Priscilla; Akulenko Natalja; Pavlova Olga; Padovan Julio C; Chait Brian T; Washburn Michael P; Ackermann Hans-W; Mushegian Arcady; Gabisonia Tarasii; Molineux Ian; Severinov Konstantin
Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.
Journal of molecular biology 2008;377(3):774-89.
2008: Westblade Lars F; Minakhin Leonid; Kuznedelov Konstantin; Tackett Alan J; Chang Emmanuel J; Mooney Rachel A; Vvedenskaya Irina; Wang Qing Jun; Fenyö David; Rout Michael P; Landick Robert; Chait Brian T; Severinov Konstantin; Darst Seth A
Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions.
Journal of proteome research 2008;7(3):1244-50.
2008: Bogdanova Ekaterina; Djordjevic Marko; Papapanagiotou Ioanna; Heyduk Tomasz; Kneale Geoff; Severinov Konstantin
Transcription regulation of the type II restriction-modification system AhdI.
Nucleic acids research 2008;36(5):1429-42.
2008: Yuzenkova Yulia; Zenkin Nikolay; Severinov Konstantin
Mapping of RNA polymerase residues that interact with bacteriophage Xp10 transcription antitermination factor p7.
Journal of molecular biology 2008;375(1):29-35.
2007: Stepanova Ekaterina; Lee Jookyung; Ozerova Maria; Semenova Ekaterina; Datsenko Kirill; Wanner Barry L; Severinov Konstantin; Borukhov Sergei
Analysis of promoter targets for Escherichia coli transcription elongation factor GreA in vivo and in vitro.
Journal of bacteriology 2007;189(24):8772-85.
2007: Novikova Maria; Metlitskaya Anastasia; Datsenko Kirill; Kazakov Teymur; Kazakov Alexey; Wanner Barry; Severinov Konstantin
The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C.
Journal of bacteriology 2007;189(22):8361-5.
2007: Sobolev Alexander; Severinov Konstantin; Kovalchuk Mikhail; Feigel'man Mikhail; Frumkina Rebecca; Kuhn Jens; Sagdeev Roald; Lyakhovich Simon; Antipov Evgeny
Russian science: what the scientists say.
Nature 2007;449(7162):528-30.
2007: Severinov Konstantin; Semenova Ekaterina; Kazakov Alexey; Kazakov Teymur; Gelfand Mikhail S
Low-molecular-weight post-translationally modified microcins.
Molecular microbiology 2007;65(6):1380-94.
2007: Phadtare Sangita; Kazakov Teymur; Bubunenko Mikhail; Court Donald L; Pestova Tatyana; Severinov Konstantin
Transcription antitermination by translation initiation factor IF1.
Journal of bacteriology 2007;189(11):4087-93.
2007: Dailidiene Daiva; Tan Shumin; Ogura Keiji; Zhang Maojun; Lee Amy H; Severinov Konstantin; Berg Douglas E
Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits.
Helicobacter 2007;12(2):103-11.
2007: Kazakov Teymur; Metlitskaya Anastasia; Severinov Konstantin
Amino acid residues required for maturation, cell uptake, and processing of translation inhibitor microcin C.
Journal of bacteriology 2007;189(5):2114-8.
2007: Zenkin Nikolay; Kulbachinskiy Andrey; Yuzenkova Yuliya; Mustaev Arkady; Bass Irina; Severinov Konstantin; Brodolin Konstantin
Region 1.2 of the RNA polymerase sigma subunit controls recognition of the -10 promoter element.
The EMBO journal 2007;26(4):955-64.
2007: Sevostyanova Anastasiya; Djordjevic Marko; Kuznedelov Konstantin; Naryshkina Tatyana; Gelfand Mikhail S; Severinov Konstantin; Minakhin Leonid
Temporal regulation of viral transcription during development of Thermus thermophilus bacteriophage phiYS40.
Journal of molecular biology 2007;366(2):420-35.
2006: Naryshkina Tatyana; Liu Jing; Florens Laurence; Swanson Selene K; Pavlov Andrey R; Pavlova Nadejda V; Inman Ross; Minakhin Leonid; Kozyavkin Sergei A; Washburn Michael; Mushegian Arcady; Severinov Konstantin
Thermus thermophilus bacteriophage phiYS40 genome and proteomic characterization of virions.
Journal of molecular biology 2006;364(4):667-77.
2006: Baxter Kimberly; Lee Jennifer; Minakhin Leonid; Severinov Konstantin; Hinton Deborah M
Mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage T4 transcription factors AsiA and MotA.
Journal of molecular biology 2006;363(5):931-44.
2006: Djordjevic Marko; Semenova Ekaterina; Shraiman Boris; Severinov Konstantin
Quantitative analysis of a virulent bacteriophage transcription strategy.
Virology 2006;354(2):240-51.
2006: Kuznedelov K D; Komissarova N V; Severinov K V
The role of the bacterial RNA polymerase beta subunit flexible flap domain in transcription termination.
Doklady. Biochemistry and biophysics 2006;410():263-6.
2006: Naryshkina Tatyana; Kuznedelov Konstantin; Severinov Konstantin
The role of the largest RNA polymerase subunit lid element in preventing the formation of extended RNA-DNA hybrid.
Journal of molecular biology 2006;361(4):634-43.
2006: Zenkin Nikolay; Yuzenkova Yulia; Severinov Konstantin
Transcript-assisted transcriptional proofreading.
Science (New York, N.Y.) 2006;313(5786):518-20.
2006: Feklistov Andrey; Barinova Nataliya; Sevostyanova Anastasiya; Heyduk Ewa; Bass Irina; Vvedenskaya Irina; Kuznedelov Konstantin; Merkiene Egle; Stavrovskaya Elena; Klimasauskas Saulius; Nikiforov Vadim; Heyduk Tomasz; Severinov Konstantin; Kulbachinskiy Andrey
A basal promoter element recognized by free RNA polymerase sigma subunit determines promoter recognition by RNA polymerase holoenzyme.
Molecular cell 2006;23(1):97-107.
2006: Wigneshweraraj Siva R; Savalia Dhruti; Severinov Konstantin; Buck Martin
Interplay between the beta' clamp and the beta' jaw domains during DNA opening by the bacterial RNA polymerase at sigma54-dependent promoters.
Journal of molecular biology 2006;359(5):1182-95.
2006: Metlitskaya Anastasia; Kazakov Teymur; Kommer Aigar; Pavlova Olga; Praetorius-Ibba Mette; Ibba Michael; Krasheninnikov Igor; Kolb Vyacheslav; Khmel Inessa; Severinov Konstantin
Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic Microcin C.
The Journal of biological chemistry 2006;281(26):18033-42.
2006: Severinova Elena; Severinov Konstantin
Localization of the Escherichia coli RNA polymerase beta' subunit residue phosphorylated by bacteriophage T7 kinase Gp0.7.
Journal of bacteriology 2006;188(10):3470-6.
2006: de Cristóbal Ricardo E; Solbiati Jose O; Zenoff Ana M; Vincent Paula A; Salomón Raul A; Yuzenkova Julia; Severinov Konstantin; Farías Ricardo N
Microcin J25 uptake: His5 of the MccJ25 lariat ring is involved in interaction with the inner membrane MccJ25 transporter protein SbmA.
Journal of bacteriology 2006;188(9):3324-8.
2006: Kuznedelov Konstantin; Lamour Valerie; Patikoglou Georgia; Chlenov Mark; Darst Seth A; Severinov Konstantin
Recombinant Thermus aquaticus RNA polymerase for structural studies.
Journal of molecular biology 2006;359(1):110-21.
2006: Heyduk Ewa; Kuznedelov Konstantin; Severinov Konstantin; Heyduk Tomasz
A consensus adenine at position -11 of the nontemplate strand of bacterial promoter is important for nucleation of promoter melting.
The Journal of biological chemistry 2006;281(18):12362-9.
2006: Phadtare Sangita; Tadigotla Vasisht; Shin Weon-Hye; Sengupta Anirvan; Severinov Konstantin
Analysis of Escherichia coli global gene expression profiles in response to overexpression and deletion of CspC and CspE.
Journal of bacteriology 2006;188(7):2521-7.
2006: Zenkin Nikolay; Naryshkina Tatyana; Kuznedelov Konstantin; Severinov Konstantin
The mechanism of DNA replication primer synthesis by RNA polymerase.
Nature 2006;439(7076):617-20.
2005: Minakhin Leonid; Semenova Ekaterina; Liu Jing; Vasilov Anatoly; Severinova Elena; Gabisonia Tarasii; Inman Ross; Mushegian Arcady; Severinov Konstantin
Genome sequence and gene expression of Bacillus anthracis bacteriophage Fah.
Journal of molecular biology 2005;354(1):1-15.
2005: Wigneshweraraj Siva R; Burrows Patricia C; Severinov Konstantin; Buck Martin
Stable DNA opening within open promoter complexes is mediated by the RNA polymerase beta'-jaw domain.
The Journal of biological chemistry 2005;280(43):36176-84.
2005: Temiakov Dmitry; Zenkin Nikolay; Vassylyeva Marina N; Perederina Anna; Tahirov Tahir H; Kashkina Ekaterina; Savkina Maria; Zorov Savva; Nikiforov Vadim; Igarashi Noriyuki; Matsugaki Naohiro; Wakatsuki Soichi; Severinov Konstantin; Vassylyev Dmitry G
Structural basis of transcription inhibition by antibiotic streptolydigin.
Molecular cell 2005;19(5):655-66.
2005: Phadtare Sangita; Severinov Konstantin
Extended -10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription.
Journal of bacteriology 2005;187(18):6584-9.
2005: Brodolin Konstantin; Zenkin Nikolay; Severinov Konstantin
Remodeling of the sigma70 subunit non-template DNA strand contacts during the final step of transcription initiation.
Journal of molecular biology 2005;350(5):930-7.
2005: Semenova Ekaterina; Yuzenkova Yulia; Peduzzi Jean; Rebuffat Sylvie; Severinov Konstantin
Structure-activity analysis of microcinJ25: distinct parts of the threaded lasso molecule are responsible for interaction with bacterial RNA polymerase.
Journal of bacteriology 2005;187(11):3859-63.
2005: Minakhin Leonid; Severinov Konstantin
Transcription regulation by bacteriophage T4 AsiA.
Protein expression and purification 2005;41(1):1-8.
2005: Nickels Bryce E; Garrity Sean J; Mekler Vladimir; Minakhin Leonid; Severinov Konstantin; Ebright Richard H; Hochschild Ann
The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(12):4488-93.
2005: Campbell Elizabeth A; Pavlova Olga; Zenkin Nikolay; Leon Fred; Irschik Herbert; Jansen Rolf; Severinov Konstantin; Darst Seth A
Structural, functional, and genetic analysis of sorangicin inhibition of bacterial RNA polymerase.
The EMBO journal 2005;24(4):674-82.
2005: Semenova Ekaterina; Djordjevic Marko; Shraiman Boris; Severinov Konstantin
The tale of two RNA polymerases: transcription profiling and gene expression strategy of bacteriophage Xp10.
Molecular microbiology 2005;55(3):764-77.
2005: Semenova Ekaterina; Minakhin Leonid; Bogdanova Ekaterina; Nagornykh Maxim; Vasilov Anatoliy; Heyduk Tomasz; Solonin Alexander; Zakharova Marina; Severinov Konstantin
Transcription regulation of the EcoRV restriction-modification system.
Nucleic acids research 2005;33(21):6942-51.
2005: Phadtare Sangita; Severinov Konstantin
Nucleic acid melting by Escherichia coli CspE.
Nucleic acids research 2005;33(17):5583-90.
2005: Sosunov Vasily; Zorov Savva; Sosunova Ekaterina; Nikolaev Anatoly; Zakeyeva Irina; Bass Irina; Goldfarb Alex; Nikiforov Vadim; Severinov Konstantin; Mustaev Arkady
The involvement of the aspartate triad of the active center in all catalytic activities of multisubunit RNA polymerase.
Nucleic acids research 2005;33(13):4202-11.
2004: Budarina Zhanna I; Nikitin Dmitri V; Zenkin Nikolay; Zakharova Marina; Semenova Ekaterina; Shlyapnikov Michael G; Rodikova Ekaterina A; Masyukova Svetlana; Ogarkov Oleg; Baida Gleb E; Solonin Alexander S; Severinov Konstantin
A new Bacillus cereus DNA-binding protein, HlyIIR, negatively regulates expression of B. cereus haemolysin II.
Microbiology (Reading, England) 2004;150(Pt 11):3691-701.
2004: Burrows Patricia C; Severinov Konstantin; Buck Martin; Wigneshweraraj Siva R
Reorganisation of an RNA polymerase-promoter DNA complex for DNA melting.
The EMBO journal 2004;23(21):4253-63.
2004: Wigneshweraraj Siva R; Burrows Patricia C; Nechaev Sergei; Zenkin Nikolay; Severinov Konstantin; Buck Martin
Regulated communication between the upstream face of RNA polymerase and the beta' subunit jaw domain.
The EMBO journal 2004;23(21):4264-74.
2004: King Rodney A; Markov Dmitry; Sen Ranjan; Severinov Konstantin; Weisberg Robert A
A conserved zinc binding domain in the largest subunit of DNA-dependent RNA polymerase modulates intrinsic transcription termination and antitermination but does not stabilize the elongation complex.
Journal of molecular biology 2004;342(4):1143-54.
2004: Markov Dmitry; Christie Gail E; Sauer Brian; Calendar Richard; Park Taehyun; Young Ry; Severinov Konstantin
P2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC.
Journal of bacteriology 2004;186(14):4628-37.
2004: Adelman Karen; Yuzenkova Julia; La Porta Arthur; Zenkin Nikolay; Lee Jookyung; Lis John T; Borukhov Sergei; Wang Michelle D; Severinov Konstantin
Molecular mechanism of transcription inhibition by peptide antibiotic Microcin J25.
Molecular cell 2004;14(6):753-62.
2004: Gregory B D; Nickels B E; Garrity S J; Severinova E; Minakhin L; Urbauer R J Bieber; Urbauer J L; Heyduk T; Severinov K; Hochschild A
A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(13):4554-9.
2004: Zenkin Nikolay; Severinov Konstantin
The role of RNA polymerase sigma subunit in promoter-independent initiation of transcription.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(13):4396-400.
2004: Phadtare Sangita; Inouye Masayori; Severinov Konstantin
The mechanism of nucleic acid melting by a CspA family protein.
Journal of molecular biology 2004;337(1):147-55.
2004: Zakharova Marina; Minakhin Leonid; Solonin Alexander; Severinov Konstantin
Regulation of RNA polymerase promoter selectivity by covalent modification of DNA.
Journal of molecular biology 2004;335(1):103-11.
2003: Naryshkina Tatyana; Bruning Adrian; Gadal Olivier; Severinov Konstantin
Role of second-largest RNA polymerase I subunit Zn-binding domain in enzyme assembly.
Eukaryotic cell 2003;2(5):1046-52.
2003: Phadtare Sangita; Hwang Jihwan; Severinov Konstantin; Inouye Masayori
CspB and CspL, thermostable cold-shock proteins from Thermotoga maritima.
Genes to cells : devoted to molecular & cellular mechanisms 2003;8(10):801-10.
2003: Wilson Kelly-Anne; Kalkum Markus; Ottesen Jennifer; Yuzenkova Julia; Chait Brian T; Landick Robert; Muir Tom; Severinov Konstantin; Darst Seth A
Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail.
Journal of the American Chemical Society 2003;125(41):12475-83.
2003: Minakhin Leonid; Severinov Konstantin
On the role of the Escherichia coli RNA polymerase sigma 70 region 4.2 and alpha-subunit C-terminal domains in promoter complex formation on the extended -10 galP1 promoter.
The Journal of biological chemistry 2003;278(32):29710-8.
2003: Burrows Patricia C; Severinov Konstantin; Ishihama Akira; Buck Martin; Wigneshweraraj Siva R
Mapping sigma 54-RNA polymerase interactions at the -24 consensus promoter element.
The Journal of biological chemistry 2003;278(32):29728-43.
2003: Yuzenkova Julia; Nechaev Sergei; Berlin Jana; Rogulja Dragana; Kuznedelov Konstantin; Inman Ross; Mushegian Arcady; Severinov Konstantin
Genome of Xanthomonas oryzae bacteriophage Xp10: an odd T-odd phage.
Journal of molecular biology 2003;330(4):735-48.
2003: Minakhin Leonid; Niedziela-Majka Anita; Kuznedelov Konstantin; Adelman Karen; Urbauer Jeffrey L; Heyduk Tomasz; Severinov Konstantin
Interaction of T4 AsiA with its target sites in the RNA polymerase sigma70 subunit leads to distinct and opposite effects on transcription.
Journal of molecular biology 2003;326(3):679-90.
2003: Wigneshweraraj Siva R; Kuznedelov Konstantin; Severinov Konstantin; Buck Martin
Multiple roles of the RNA polymerase beta subunit flap domain in sigma 54-dependent transcription.
The Journal of biological chemistry 2003;278(5):3455-65.
2003: Phadtare Sangita; Severinov Konstantin; Inouye Masayori
Assay of transcription antitermination by proteins of the CspA family.
Methods in enzymology 2003;371():460-71.
2003: Mustaev Arkadv; Zaychikov Eugeny; Grachev Mikhail; Kozlov Maxim; Severinov Konstantin; Epshtein Vitaly; Korzheva Nataliya; Bereshchenko Oxana; Markovtsov Vadim; Lukhtanov Eugeny; Tsarev Igor; Maximova Tatyana; Kashlev Mikhail; Bass Irina; Nikiforov Vadim; Goldfarb Alex
Strategies and methods of cross-linking of RNA polymerase active center.
Methods in enzymology 2003;371():191-206.
2003: Wigneshweraraj Siva R; Nechaev Sergei; Bordes Patricia; Jones Susan; Cannon Wendy; Severinov Konstantin; Buck Martin
Enhancer-dependent transcription by bacterial RNA polymerase: the beta subunit downstream lobe is used by sigma 54 during open promoter complex formation.
Methods in enzymology 2003;370():646-57.
2003: Nechaev Sergei; Imburgio Diane; Severinov Konstantin
Purification and characterization of bacteriophage-encoded inhibitors of host RNA polymerase: T-odd phage gp2-like proteins.
Methods in enzymology 2003;370():212-25.
2003: Kuznedelov Konstantin; Minakhin Leonid; Severinov Konstantin
Preparation and characterization of recombinant Thermus aquaticus RNA polymerase.
Methods in enzymology 2003;370():94-108.
2003: Nechaev Sergei; Severinov Konstantin
Bacteriophage-induced modifications of host RNA polymerase.
Annual review of microbiology 2003;57():301-22.
2002: Yuzenkova Julia; Delgado Monica; Nechaev Sergei; Savalia Dhruti; Epshtein Vitaly; Artsimovitch Irina; Mooney Rachel A; Landick Robert; Farias Ricardo N; Salomon Raul; Severinov Konstantin
Mutations of bacterial RNA polymerase leading to resistance to microcin j25.
The Journal of biological chemistry 2002;277(52):50867-75.
2002: Borukhov Sergei; Severinov Konstantin
Role of the RNA polymerase sigma subunit in transcription initiation.
Research in microbiology 2002;153(9):557-62.
2002: Phadtare Sangita; Tyagi Sanjay; Inouye Masayori; Severinov Konstantin
Three amino acids in Escherichia coli CspE surface-exposed aromatic patch are critical for nucleic acid melting activity leading to transcription antitermination and cold acclimation of cells.
The Journal of biological chemistry 2002;277(48):46706-11.
2002: Wigneshweraraj Siva R; Nechaev Sergei; Severinov Konstantin; Buck Martin
Beta subunit residues 186-433 and 436-445 are commonly used by Esigma54 and Esigma70 RNA polymerase for open promoter complex formation.
Journal of molecular biology 2002;319(5):1067-83.
2002: Nechaev Sergei; Yuzenkova Yulia; Niedziela-Majka Anita; Heyduk Tomasz; Severinov Konstantin
A novel bacteriophage-encoded RNA polymerase binding protein inhibits transcription initiation and abolishes transcription termination by host RNA polymerase.
Journal of molecular biology 2002;320(1):11-22.
2002: Kuznedelov Konstantin; Korzheva Nataliya; Mustaev Arkady; Severinov Konstantin
Structure-based analysis of RNA polymerase function: the largest subunit's rudder contributes critically to elongation complex stability and is not involved in the maintenance of RNA-DNA hybrid length.
The EMBO journal 2002;21(6):1369-78.
2002: Phadtare Sangita; Inouye Masayori; Severinov Konstantin
The nucleic acid melting activity of Escherichia coli CspE is critical for transcription antitermination and cold acclimation of cells.
The Journal of biological chemistry 2002;277(9):7239-45.
2002: Kuznedelov Konstantin; Minakhin Leonid; Niedziela-Majka Anita; Dove Simon L; Rogulja Dragana; Nickels Bryce E; Hochschild Ann; Heyduk Tomasz; Severinov Konstantin
A role for interaction of the RNA polymerase flap domain with the sigma subunit in promoter recognition.
Science (New York, N.Y.) 2002;295(5556):855-7.
2001: Naryshkina T; Mustaev A; Darst S A; Severinov K
The beta ' subunit of Escherichia coli RNA polymerase is not required for interaction with initiating nucleotide but is necessary for interaction with rifampicin.
The Journal of biological chemistry 2001;276(16):13308-13.
2001: Minakhin L; Camarero J A; Holford M; Parker C; Muir T W; Severinov K
Mapping the molecular interface between the sigma(70) subunit of E. coli RNA polymerase and T4 AsiA.
Journal of molecular biology 2001;306(4):631-42.
2001: Minakhin L; Bhagat S; Brunning A; Campbell E A; Darst S A; Ebright R H; Severinov K
Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(3):892-7.
2001: Severinov K
T7 RNA polymerase transcription complex: what you see is not what you get.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(1):5-7.
2001: Minakhin L; Nechaev S; Campbell E A; Severinov K
Recombinant Thermus aquaticus RNA polymerase, a new tool for structure-based analysis of transcription.
Journal of bacteriology 2001;183(1):71-6.
2000: Mah T F; Kuznedelov K; Mushegian A; Severinov K; Greenblatt J
The alpha subunit of E. coli RNA polymerase activates RNA binding by NusA.
Genes & development 2000;14(20):2664-75.
2000: Naryshkina T; Rogulja D; Golub L; Severinov K
Inter- and intrasubunit interactions during the formation of RNA polymerase assembly intermediate.
The Journal of biological chemistry 2000;275(40):31183-90.
2000: Nechaev S; Chlenov M; Severinov K
Dissection of two hallmarks of the open promoter complex by mutation in an RNA polymerase core subunit.
The Journal of biological chemistry 2000;275(33):25516-22.
2000: Bae W; Xia B; Inouye M; Severinov K
Escherichia coli CspA-family RNA chaperones are transcription antiterminators.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(14):7784-9.
2000: Brodolin K; Mustaev A; Severinov K; Nikiforov V
Identification of RNA polymerase beta' subunit segment contacting the melted region of the lacUV5 promoter.
The Journal of biological chemistry 2000;275(5):3661-6.
2000: Opalka N; Mooney R A; Richter C; Severinov K; Landick R; Darst S A
Direct localization of a beta-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(2):617-22.
1999: Markov D; Naryshkina T; Mustaev A; Severinov K
A zinc-binding site in the largest subunit of DNA-dependent RNA polymerase is involved in enzyme assembly.
Genes & development 1999;13(18):2439-48.
1999: Zhang G; Campbell E A; Minakhin L; Richter C; Severinov K; Darst S A
Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution.
Cell 1999;98(6):811-24.
1999: Zakharova N; Paster B J; Wesley I; Dewhirst F E; Berg D E; Severinov K V
Fused and overlapping rpoB and rpoC genes in Helicobacters, Campylobacters, and related bacteria.
Journal of bacteriology 1999;181(12):3857-9.
1999: Raudonikiene A; Zakharova N; Su W W; Jeong J Y; Bryden L; Hoffman P S; Berg D E; Severinov K
Helicobacter pylori with separate beta- and beta'-subunits of RNA polymerase is viable and can colonize conventional mice.
Molecular microbiology 1999;32(1):131-8.
1999: Nedea E C; Markov D; Naryshkina T; Severinov K
Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature.
Journal of bacteriology 1999;181(8):2663-5.
1999: Bae W; Phadtare S; Severinov K; Inouye M
Characterization of Escherichia coli cspE, whose product negatively regulates transcription of cspA, the gene for the major cold shock protein.
Molecular microbiology 1999;31(5):1429-41.
1998: Zakharova N; Bass I; Arsenieva E; Nikiforov V; Severinov K
Mutations in and monoclonal antibody binding to evolutionary hypervariable region of Escherichia coli RNA polymerase beta' subunit inhibit transcript cleavage and transcript elongation.
The Journal of biological chemistry 1998;273(38):24912-20.
1998: Zakharova N; Hoffman P S; Berg D E; Severinov K
The largest subunits of RNA polymerase from gastric helicobacters are tethered.
The Journal of biological chemistry 1998;273(31):19371-4.
1998: Muzzin O; Campbell E A; Xia L; Severinova E; Darst S A; Severinov K
Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity.
The Journal of biological chemistry 1998;273(24):15157-61.
1998: Severinova E; Severinov K; Darst S A
Inhibition of Escherichia coli RNA polymerase by bacteriophage T4 AsiA.
Journal of molecular biology 1998;279(1):9-18.
1997: Severinov K; Darst S A
A mutant RNA polymerase that forms unusual open promoter complexes.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(25):13481-6.
1997: Severinov K; Mooney R; Darst S A; Landick R
Tethering of the large subunits of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1997;272(39):24137-40.
1997: Wang Y; Severinov K; Loizos N; Fenyö D; Heyduk E; Heyduk T; Chait B T; Darst S A
Determinants for Escherichia coli RNA polymerase assembly within the beta subunit.
Journal of molecular biology 1997;270(5):648-62.
1997: Wang D; Severinov K; Landick R
Preferential interaction of the his pause RNA hairpin with RNA polymerase beta subunit residues 904-950 correlates with strong transcriptional pausing.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(16):8433-8.
1996: Severinova E; Severinov K; Fenyö D; Marr M; Brody E N; Roberts J W; Chait B T; Darst S A
Domain organization of the Escherichia coli RNA polymerase sigma 70 subunit.
Journal of molecular biology 1996;263(5):637-47.
1996: Severinov K; Mustaev A; Kukarin A; Muzzin O; Bass I; Darst S A; Goldfarb A
Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1996;271(44):27969-74.
1996: Heyduk T; Heyduk E; Severinov K; Tang H; Ebright R H
Determinants of RNA polymerase alpha subunit for interaction with beta, beta', and sigma subunits: hydroxyl-radical protein footprinting.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(19):10162-6.
1996: Kashlev M; Nudler E; Severinov K; Borukhov S; Komissarova N; Goldfarb A
Histidine-tagged RNA polymerase of Escherichia coli and transcription in solid phase.
Methods in enzymology 1996;274():326-34.
1996: Tang H; Kim Y; Severinov K; Goldfarb A; Ebright R H
Escherichia coli RNA polymerase holoenzyme: rapid reconstitution from recombinant alpha, beta, beta', and sigma subunits.
Methods in enzymology 1996;273():130-4.
1995: Severinov K; Mustaev A; Severinova E; Kozlov M; Darst S A; Goldfarb A
The beta subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1995;270(49):29428-32.
1995: Severinov K; Markov D; Severinova E; Nikiforov V; Landick R; Darst S A; Goldfarb A
Streptolydigin-resistant mutants in an evolutionarily conserved region of the beta' subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1995;270(41):23926-9.
1995: Tang H; Severinov K; Goldfarb A; Ebright R H
Rapid RNA polymerase genetics: one-day, no-column preparation of reconstituted recombinant Escherichia coli RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(11):4902-6.
1995: Severinov K; Mustaev A; Severinova E; Bass I; Kashlev M; Landick R; Nikiforov V; Goldfarb A; Darst S A
Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(10):4591-5.
1994: Tang H; Severinov K; Goldfarb A; Fenyo D; Chait B; Ebright R H
Location, structure, and function of the target of a transcriptional activator protein.
Genes & development 1994;8(24):3058-67.
1994: Mustaev A; Zaychikov E; Severinov K; Kashlev M; Polyakov A; Nikiforov V; Goldfarb A
Topology of the RNA polymerase active center probed by chimeric rifampicin-nucleotide compounds.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(25):12036-40.
1994: Severinov K; Goldfarb A
Topology of the product binding site in RNA polymerase revealed by transcript slippage at the phage lambda PL promoter.
The Journal of biological chemistry 1994;269(50):31701-5.
1994: Severinov K; Fenyö D; Severinova E; Mustaev A; Chait B T; Goldfarb A; Darst S A
The sigma subunit conserved region 3 is part of "5'-face" of active center of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1994;269(33):20826-8.
1994: Severinov K; Soushko M; Goldfarb A; Nikiforov V
RifR mutations in the beginning of the Escherichia coli rpoB gene.
Molecular & general genetics : MGG 1994;244(2):120-6.
1994: Severinov K; Kashlev M; Severinova E; Bass I; McWilliams K; Kutter E; Nikiforov V; Snyder L; Goldfarb A
A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor Alc.
The Journal of biological chemistry 1994;269(19):14254-9.
1993: Kashlev M; Martin E; Polyakov A; Severinov K; Nikiforov V; Goldfarb A
Histidine-tagged RNA polymerase: dissection of the transcription cycle using immobilized enzyme.
Gene 1993;130(1):9-14.
1993: Severinov K; Soushko M; Goldfarb A; Nikiforov V
Rifampicin region revisited. New rifampicin-resistant and streptolydigin-resistant mutants in the beta subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1993;268(20):14820-5.
1992: Severinov K; Mustaev A; Kashlev M; Borukhov S; Nikiforov V; Goldfarb A
Dissection of the beta subunit in the Escherichia coli RNA polymerase into domains by proteolytic cleavage.
The Journal of biological chemistry 1992;267(18):12813-9.
1991: Borukhov S; Severinov K; Kashlev M; Lebedev A; Bass I; Rowland G C; Lim P P; Glass R E; Nikiforov V; Goldfarb A
Mapping of trypsin cleavage and antibody-binding sites and delineation of a dispensable domain in the beta subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1991;266(35):23921-6.

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