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John Shanklin

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Chemicals & Drugs

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Publications

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Carl Andre; Sung Won Kim; Xiao-Hong Yu; John Shanklin
Fusing catalase to an alkane-producing enzyme maintains enzymatic activity by converting the inhibitory byproduct H2O2 to the cosubstrate O2.
Jilian Fan; Jörg Schwender; John Shanklin; Carl Andre; Chengshi Yan; Changcheng Xu
Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii.
Carl Andre; Richard P Haslam; John Shanklin
Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus.

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All Publications

2013: Carl Andre; Sung Won Kim; Xiao-Hong Yu; John Shanklin
Fusing catalase to an alkane-producing enzyme maintains enzymatic activity by converting the inhibitory byproduct H2O2 to the cosubstrate O2.
Proceedings of the National Academy of Sciences of the United States of America 2013;110(8):3191-6.
2012: Jilian Fan; Jörg Schwender; John Shanklin; Carl Andre; Chengshi Yan; Changcheng Xu
Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii.
Plant & cell physiology 2012;53(8):1380-90.
2012: Carl Andre; Richard P Haslam; John Shanklin
Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(25):10107-12.
2011: Jodie E Guy; Johan Lengqvist; Ylva Lindqvist; Martin Moche; Edward Whittle; John Shanklin
Remote control of regioselectivity in acyl-acyl carrier protein-desaturases.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(40):16594-9.
2010: Huu Tam Nguyen; Girish Mishra; Edward Whittle; Scott A Bevan; Ann Owens Merlo; Terence A Walsh; Mark S Pidkowich; John Shanklin
Metabolic engineering of seeds can achieve levels of omega-7 fatty acids comparable with the highest levels found in natural plant sources.
Plant physiology 2010;154(4):1897-904.
2010: Amy E Tremblay; Nigel Tan; Edward Whittle; Derek J Hodgson; Brian Dawson; Peter H Buist; John Shanklin
Stereochemistry of 10-sulfoxidation catalyzed by a soluble Delta9 desaturase.
Organic & biomolecular chemistry 2010;8(6):1322-8.
2009: John Shanklin; Jodie E Guy; Girish Mishra; Ylva Lindqvist
Desaturases: emerging models for understanding functional diversification of diiron-containing enzymes.
The Journal of biological chemistry 2009;284(28):18559-63.
2008: Edward Whittle; Amy E Tremblay; Peter H Buist; John Shanklin
Revealing the catalytic potential of an acyl-ACP desaturase: tandem selective oxidation of saturated fatty acids.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(38):14738-43.
2007: Jodie E Guy; Edward Whittle; Desigan Kumaran; Ylva Lindqvist; John Shanklin
The crystal structure of the ivy Delta4-16:0-ACP desaturase reveals structural details of the oxidized active site and potential determinants of regioselectivity.
The Journal of biological chemistry 2007;282(27):19863-71.
2007: Amy E Tremblay; Edward Whittle; Peter H Buist; John Shanklin
Stereochemistry of Delta4 dehydrogenation catalyzed by an ivy (Hedera helix) Delta9 desaturase homolog.
Organic & biomolecular chemistry 2007;5(8):1270-5.
2007: Mark S Pidkowich; Huu Tam Nguyen; Ingo Heilmann; Till Ischebeck; John Shanklin
Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(11):4742-7.
2007: Kimberly M Mayer; John Shanklin
Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach.
BMC plant biology 2007;7():1.
2007: Aardra Kachroo; John Shanklin; Edward Whittle; Ludmila Lapchyk; David F Hildebrand; Pradeep Kachroo
The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis.
Plant molecular biology 2007;63(2):257-71.
2006: Jodie E Guy; Isabel A Abreu; Martin Moche; Ylva Lindqvist; Edward Whittle; John Shanklin
A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(46):17220-4.
2006: Amy E Tremblay; Peter H Buist; Derek Hodgson; Brian Dawson; Edward Whittle; John Shanklin
In vitro enzymatic oxidation of a fluorine-tagged sulfido substrate analogue: a 19F NMR investigation.
Magnetic resonance in chemistry : MRC 2006;44(6):629-32.
2005: Edward Whittle; Edgar B Cahoon; Satyam Subrahmanyam; John Shanklin
A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products.
The Journal of biological chemistry 2005;280(31):28169-76.
2005: Kimberly M Mayer; John Shanklin
A structural model of the plant acyl-acyl carrier protein thioesterase FatB comprises two helix/4-stranded sheet domains, the N-terminal domain containing residues that affect specificity and the C-terminal domain containing catalytic residues.
The Journal of biological chemistry 2005;280(5):3621-7.
2005: Roman Davydov; Behnaz Behrouzian; Stoyan K Smoukov; JoAnne Stubbe; Brian M Hoffman; John Shanklin
Effect of substrate on the diiron(III) site in stearoyl acyl carrier protein delta 9-desaturase as disclosed by cryoreduction electron paramagnetic resonance/electron nuclear double resonance spectroscopy.
Biochemistry 2005;44(4):1309-15.
2005: Kimberly M Mayer; Sean R McCorkle; John Shanklin
Linking enzyme sequence to function using Conserved Property Difference Locator to identify and annotate positions likely to control specific functionality.
BMC bioinformatics 2005;6():284.
2004: Ingo Heilmann; Sergei Mekhedov; Barbara King; John Browse; John Shanklin
Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype.
Plant physiology 2004;136(4):4237-45.
2004: Ingo Heilmann; Mark S Pidkowich; Thomas Girke; John Shanklin
Switching desaturase enzyme specificity by alternate subcellular targeting.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(28):10266-71.
2003: Martin Moche; John Shanklin; Alokesh Ghoshal; Ylva Lindqvist
Azide and acetate complexes plus two iron-depleted crystal structures of the di-iron enzyme delta9 stearoyl-acyl carrier protein desaturase. Implications for oxygen activation and catalytic intermediates.
The Journal of biological chemistry 2003;278(27):25072-80.
2003: John Shanklin; Edward Whittle
Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family.
FEBS letters 2003;545(2-3):188-92.
2002: John A Broadwater; Edward Whittle; John Shanklin
Desaturation and hydroxylation. Residues 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity.
The Journal of biological chemistry 2002;277(18):15613-20.
2002: Behnaz Behrouzian; Christopher K Savile; Brian Dawson; Peter H Buist; John Shanklin
Exploring the hydroxylation-dehydrogenation connection: novel catalytic activity of castor stearoyl-ACP Delta(9) desaturase.
Journal of the American Chemical Society 2002;124(13):3277-83.
2001: Pradeep Kachroo; John Shanklin; Jyoti Shah; Edward Whittle; Daniel F Klessig
A fatty acid desaturase modulates the activation of defense signaling pathways in plants.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(16):9448-53.
2001: Edward Whittle; John Shanklin
Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase.
The Journal of biological chemistry 2001;276(24):21500-5.
2000: Edgar B Cahoon; John Shanklin
Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(22):12350-5.
2000: John Shanklin
Exploring the possibilities presented by protein engineering.
Current opinion in plant biology 2000;3(3):243-8.
2000: John Shanklin
Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form.
Protein expression and purification 2000;18(3):355-60.
1998: Pierre Broun; John Shanklin; Edward Whittle; chris somerville
Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids.
Science (New York, N.Y.) 1998;282(5392):1315-7.
1998: Edgar B Cahoon; S Shah; John Shanklin; John Browse
A determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed.
Plant physiology 1998;117(2):593-8.
1997: Edgar B Cahoon; Ylva Lindqvist; G Schneider; John Shanklin
Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(10):4872-7.
1997: Edgar B Cahoon; Sean J Coughlan; John Shanklin
Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed.
Plant molecular biology 1997;33(6):1105-10.
1997: John Shanklin; Catalina Achim; H Schmidt; Brian G Fox; Eckard Münck
Mössbauer studies of alkane omega-hydroxylase: evidence for a diiron cluster in an integral-membrane enzyme.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(7):2981-6.
1996: Ylva Lindqvist; W Huang; Gunter Schneider; John Shanklin
Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins.
The EMBO journal 1996;15(16):4081-92.
1996: D J Schultz; Edgar B Cahoon; John Shanklin; R Craig; D L Cox-Foster; R O Mumma; J I Medford
Expression of a delta 9 14:0-acyl carrier protein fatty acid desaturase gene is necessary for the production of omega 5 anacardic acids found in pest-resistant geranium (Pelargonium xhortorum).
Proceedings of the National Academy of Sciences of the United States of America 1996;93(16):8771-5.
1996: Edgar B Cahoon; L A Mills; John Shanklin
Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin.
Journal of bacteriology 1996;178(3):936-9.
1995: John Shanklin; N D DeWitt; John M Flanagan
The stroma of higher plant plastids contain ClpP and ClpC, functional homologs of Escherichia coli ClpP and ClpA: an archetypal two-component ATP-dependent protease.
The Plant cell 1995;7(10):1713-22.
1995: John M Flanagan; Joseph S Wall; M S Capel; D K Schneider; John Shanklin
Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore.
Biochemistry 1995;34(34):10910-7.
1994: Edgar B Cahoon; A M Cranmer; John Shanklin; John B Ohlrogge
delta 6 Hexadecenoic acid is synthesized by the activity of a soluble delta 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm.
The Journal of biological chemistry 1994;269(44):27519-26.
1994: John Shanklin; E Whittle; Brian G Fox
Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase.
Biochemistry 1994;33(43):12787-94.
1994: Brian G Fox; John Shanklin; J Ai; Thomas M Loehr; Joann Sanders-Loehr
Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins.
Biochemistry 1994;33(43):12776-86.
1994: Edgar B Cahoon; C K Becker; John Shanklin; John B Ohlrogge
cDNAs for isoforms of the delta 9-stearoyl-acyl carrier protein desaturase from Thunbergia alata endosperm.
Plant physiology 1994;106(2):807-8.
1993: Brian G Fox; John Shanklin; chris somerville; Eckard Münck
Stearoyl-acyl carrier protein delta 9 desaturase from Ricinus communis is a diiron-oxo protein.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(6):2486-90.
1992: Edgar B Cahoon; John Shanklin; John B Ohlrogge
Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(23):11184-8.
1992: Gunter Schneider; Ylva Lindqvist; John Shanklin; C Somerville
Preliminary crystallographic data for stearoyl-acyl carrier protein desaturase from castor seed.
Journal of molecular biology 1992;225(2):561-4.
1991: John Shanklin; C Mullins; chris somerville
Sequence of a Complementary DNA from Cucumis sativus L. Encoding the Stearoyl-Acyl-Carrier Protein Desaturase.
Plant physiology 1991;97(1):467-8.
1991: John Shanklin; chris somerville
Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(6):2510-4.
1989: M Jabben; John Shanklin; Richard D Vierstra
Red light-induced accumulation of ubiquitin-phytochrome conjugates in both monocots and dicots.
Plant physiology 1989;90(2):380-4.
1987: John Shanklin; M Jabben; Richard D Vierstra
Red light-induced formation of ubiquitin-phytochrome conjugates: Identification of possible intermediates of phytochrome degradation.
Proceedings of the National Academy of Sciences of the United States of America 1987;84(2):359-63.
1986: John Shanklin; L E Schrader
Is There a Relationship between Infection by Rhizobia and Occurrence of Disomatic Nuclei in Nodules of Alfalfa (Medicago sativa L.)?
Plant physiology 1986;80(1):280-2.