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Antonio Sillero

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María A Günther Sillero; Anabel De Diego; Antonio Sillero
Methylenebisphosphonate and triphosphate derivatives of the mevalonate pathway are substrates of yeast UTP:glucose-1-phosphate uridylyltransferase.
Francisco Garrido; Claudia Alves; Sylvie Estrela; Gabino F Sánchez-Pérez; Antonio Sillero; María Angeles Pajares
Refolding and characterization of methionine adenosyltransferase from Euglena gracilis.
Maria A Günther Sillero; Anabel de Diego; Gemma Rodríguez-Tarduchy; Antonio Sillero
Determination of enzymatic activities and metabolites in microliter sample size.

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All Publications

2012: María A Günther Sillero; Anabel De Diego; Antonio Sillero
Methylenebisphosphonate and triphosphate derivatives of the mevalonate pathway are substrates of yeast UTP:glucose-1-phosphate uridylyltransferase.
Biochimie 2012;94(9):1871-5.
2011: Francisco Garrido; Claudia Alves; Sylvie Estrela; Gabino F Sánchez-Pérez; Antonio Sillero; María Angeles Pajares
Refolding and characterization of methionine adenosyltransferase from Euglena gracilis.
Protein expression and purification 2011;79(1):128-36.
2011: Maria A Günther Sillero; Anabel de Diego; Gemma Rodríguez-Tarduchy; Antonio Sillero
Determination of enzymatic activities and metabolites in microliter sample size.
Analytical biochemistry 2011;410(1):40-3.
2010: Andrés A Maldonado; João M Ribeiro; Antonio Sillero
Isoelectric point, electric charge, and nomenclature of the acid-base residues of proteins.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology 2010;38(4):230-7.
2009: María A Günther Sillero; Anabel De Diego; Janeth E F Tavares; Joana A D Catanho da Silva; Francisco J Pérez-Zúñiga; Antonio Sillero
Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases Potential relationship with the effect of bisphosphonates on osteoclasts.
Biochemical pharmacology 2009;78(4):335-43.
2008: Francisco J Pérez-Zúñiga; María A Günther Sillero; Antonio Sillero
Bisphosphonates activate the 5-fluorouracil/uracil phosphoribosyltransferase activity present in Saccharomyces cerevisiae cell extracts: implications for tumor treatments.
Biochemical pharmacology 2008;76(7):825-30.
2008: María A Günther Sillero; Anabel De Diego; Francisco J Pérez-Zúñiga; Antonio Sillero
Synthesis of bisphosphonate derivatives of ATP by T4 DNA ligase, ubiquitin activating enzyme (E1) and other ligases.
Biochemical pharmacology 2008;75(10):1959-65.
2008: María A Günther Sillero; Francisco Pérez-Zúñiga; Joana Gomes; Ana Isabel de Carvalho; Susana Martins; Eduardo Silles; Antonio Sillero
Synthesis of FUDP-N-acetylglucosamine and FUDP-glucose in Saccharomyces cerevisiae cells treated with 5-fluorouracil.
FEMS yeast research 2008;8(2):257-65.
2008: Andrés Maldonado; Francisco Vara; Antonio Sillero
Improved application of the oscillating method for the isoelectric point determination of protein: potential connection with protein data banks.
Computers in biology and medicine 2008;38(1):23-30.
2006: María A Günther Sillero; Anabel De Diego; Eduardo Silles; Francisco Pérez-Zúñiga; Antonio Sillero
Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase.
FEBS letters 2006;580(24):5723-7.
2006: Juan A G Ranea; Antonio Sillero; Janet M Thornton; Christine A Orengo
Protein superfamily evolution and the last universal common ancestor (LUCA).
Journal of molecular evolution 2006;63(4):513-25.
2006: Antonio Sillero; Vitali A Selivanov; Marta Cascante
Pentose phosphate and calvin cycles: Similarities and three-dimensional views*.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology 2006;34(4):275-7.
2006: Russell L Marsden; Juan A G Ranea; Antonio Sillero; Oliver Redfern; Corin Yeats; Michael Maibaum; David Lee; Sarah Addou; Gabrielle A Reeves; Timothy J Dallman; Christine A Orengo
Exploiting protein structure data to explore the evolution of protein function and biological complexity.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2006;361(1467):425-40.
2006: Antonio Sillero; Andres Maldonado
Isoelectric point determination of proteins and other macromolecules: oscillating method.
Computers in biology and medicine 2006;36(2):157-66.
2005: María A Günther Sillero; Anabel De Diego; Eduardo Silles; Antonio Sillero
Synthesis of (di)nucleoside polyphosphates by the ubiquitin activating enzyme E1.
FEBS letters 2005;579(27):6223-9.
2005: Eduardo Silles; Hugo Osorio; Rita Maia; María A Günther Sillero; Antonio Sillero
Micromolar HgCl2 concentrations transitorily duplicate the ATP level in Saccharomyces cerevisiae cells.
FEBS letters 2005;579(19):4044-8.
2005: Hugo Osório; Eduardo Silles; Rita Maia; Bárbara Peleteiro; Pedro Moradas-Ferreira; María A Günther Sillero; Antonio Sillero
Influence of chronological aging on the survival and nucleotide content of Saccharomyces cerevisiae cells grown in different conditions: occurrence of a high concentration of UDP-N-acetylglucosamine in stationary cells grown in 2% glucose.
FEMS yeast research 2005;5(4-5):387-98.
2005: Frances Pearl; Annabel Todd; Ian Sillitoe; Mark Dibley; Oliver Redfern; Tony Lewis; Christopher Bennett; Russell L Marsden; Alistair Grant; David Lee; Adrian Akpor; Michael Maibaum; Andrew Harrison; Timothy J Dallman; Gabrielle Reeves; Ilhem Diboun; Sarah Addou; Stefano Lise; Caroline Johnston; Antonio Sillero; Janet Thornton; Christine Orengo
The CATH Domain Structure Database and related resources Gene3D and DHS provide comprehensive domain family information for genome analysis.
Nucleic acids research 2005;33(Database issue):D247-51.
2004: Vitaly A Selivanov; Joaquim Puigjaner; Antonio Sillero; Josep J Centelles; Antonio Ramos-Montoya; Paul W-N Lee; Marta Cascante
An optimized algorithm for flux estimation from isotopomer distribution in glucose metabolites.
Bioinformatics (Oxford, England) 2004;20(18):3387-97.
2004: Andrzej Guranowski; Anabel De Diego; Antonio Sillero; María A Günther Sillero
Uridine 5'-polyphosphates (p4U and p5U) and uridine(5')polyphospho(5')nucleosides (Up(n)Ns) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae.
FEBS letters 2004;561(1-3):83-8.
2004: Hugo Osório; Pedro Moradas-Ferreira; María A Günther Sillero; Antonio Sillero
In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration.
Archives of microbiology 2004;181(3):231-6.
2003: María A Günther Sillero; Anabel De Diego; Eduardo Silles; Hugo Osorio; Antonio Sillero
Polyphosphates strongly inhibit the tRNA dependent synthesis of poly(A) catalyzed by poly(A) polymerase from Saccharomyces cerevisiae.
FEBS letters 2003;550(1-3):41-5.
2003: Hugo Osorio; Elisabete Carvalho; Mercedes del Valle; María A Günther Sillero; Pedro Moradas-Ferreira; Antonio Sillero
H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae. An experimental and theoretical approach.
European journal of biochemistry / FEBS 2003;270(7):1578-89.
2003: Andrzej Guranowski; Antonio Sillero; María A Günther Sillero
Selective splitting of 3'-adenylated dinucleoside polyphosphates by specific enzymes degrading dinucleoside polyphosphates.
Acta biochimica Polonica 2003;50(1):123-30.
2002: María A Günther Sillero; Anabel De Diego; Hugo Osorio; Antonio Sillero
Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase.
European journal of biochemistry / FEBS 2002;269(21):5323-9.
2002: SilleroMariaA Günther; María Montes; Diego Anabel de; Valle Mercedes del; Eva Ana Atencia; Antonio Sillero
Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates.
Extremophiles : life under extreme conditions 2002;6(1):45-50.
2001: María A Günther Sillero; S Socorro; M J Baptista; M Del Valle; Anabel De Diego; Antonio Sillero
Poly(A) polymerase from Escherichia coli adenylylates the 3'-hydroxyl residue of nucleosides, nucleoside 5'-phosphates and nucleoside(5')oligophospho(5')nucleosides (NpnN).
European journal of biochemistry / FEBS 2001;268(12):3605-11.
2001: A Torrecilla; A F Marques; R D Buscalioni; Jorge Oliveira; Natércia Teixeira; Eva Ana Atencia; María A Günther Sillero; Antonio Sillero
Metabolic fate of AMP, IMP, GMP and XMP in the cytosol of rat brain: an experimental and theoretical analysis.
Journal of neurochemistry 2001;76(5):1291-307.
2000: Antonio Sillero; María A Günther Sillero
Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase and several ligases.
Pharmacology & therapeutics 2000;87(2-3):91-102.
2000: Eva Ana Atencia; M Montes; María A Günther Sillero; Antonio Sillero
Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction.
European journal of biochemistry / FEBS 2000;267(6):1707-14.
2000: Rui Fontes; J M Ribeiro; Antonio Sillero
An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism.
Acta biochimica Polonica 2000;47(1):259-68.
2000: Rui Fontes; J M Ribeiro; Antonio Sillero
Inhibition and activation of enzymes. The effect of a modifier on the reaction rate and on kinetic parameters.
Acta biochimica Polonica 2000;47(1):233-57.
1999: Eva Ana Atencia; O Madrid; María A Günther Sillero; Antonio Sillero
T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates.
European journal of biochemistry / FEBS 1999;261(3):802-11.
1999: Rui Fontes; María A Günther Sillero; Antonio Sillero
Acyl-CoA synthetase catalyzes the synthesis of diadenosine hexaphosphate (Ap6A).
Biochimie 1999;81(3):229-33.
1998: Rui Fontes; B Ortiz; Anabel De Diego; Antonio Sillero; María A Günther Sillero
Dehydroluciferyl-AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferase.
FEBS letters 1998;438(3):190-4.
1998: A F Marques; Natércia Teixeira; C Gambaretto; Antonio Sillero; María A Günther Sillero
IMP-GMP 5'-nucleotidase from rat brain: activation by polyphosphates.
Journal of neurochemistry 1998;71(3):1241-50.
1998: O Madrid; Daniel Martin; Eva Ana Atencia; Antonio Sillero; María A Günther Sillero
T4 DNA ligase synthesizes dinucleoside polyphosphates.
FEBS letters 1998;433(3):283-6.
1998: Rui Fontes; María A Günther Sillero; Antonio Sillero
Acyl coenzyme A synthetase from Pseudomonas fragi catalyzes the synthesis of adenosine 5'-polyphosphates and dinucleoside polyphosphates.
Journal of bacteriology 1998;180(12):3152-8.
1997: Rui Fontes; A Dukhovich; Antonio Sillero; María A Günther Sillero
Synthesis of dehydroluciferin by firefly luciferase: effect of dehydroluciferin, coenzyme A and nucleoside triphosphates on the luminescent reaction.
Biochemical and biophysical research communications 1997;237(2):445-50.
1997: María A Günther Sillero; O Madrid; E Zaera; Antonio Sillero
2',3'-dideoxynucleoside triphosphates (ddNTP) and di-2',3'-dideoxynucleoside tetraphosphates (ddNp4ddN) behave differently to the corresponding NTP and Np4N counterparts as substrates of firefly luciferase, dinucleoside tetraphosphatase and phosphodiesterases.
Biochimica et biophysica acta 1997;1334(2-3):191-9.
1996: A Dukhovich; Antonio Sillero; María A Günther Sillero
Time course of luciferyl adenylate synthesis in the firefly luciferase reaction.
FEBS letters 1996;395(2-3):188-90.
1995: S Garrido; E Zaera; A Torrecilla; Antonio Sillero; María A Günther Sillero
Labeled adenosine(5')tetraphospho(5')adenosine (Ap4A) and adenosine(5')tetraphospho(5')nucleoside (Ap4N). Synthesis with firefly luciferase.
Journal of biochemical and biophysical methods 1995;30(2-3):191-8.
1995: Andrzej Guranowski; E Starzyńska; María A Günther Sillero; Antonio Sillero
Conversion of adenosine(5') oligophospho(5') adenosines into inosine(5') oligophospho(5') inosines by non-specific adenylate deaminase from the snail Helix pomatia.
Biochimica et biophysica acta 1995;1243(1):78-84.
1994: S Bleisch; María A Günther Sillero; A Torrecilla; Antonio Sillero
Uric acid synthesis by rat liver supernatants from purine bases, nucleosides and nucleotides. Effect of allopurinol.
Cell biochemistry and function 1994;12(4):237-45.
1994: Andrzej Guranowski; María A Günther Sillero; Antonio Sillero
Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase.
Journal of bacteriology 1994;176(10):2986-90.
1994: J M Ribeiro; Rui Fontes; Antonio Sillero
Enzyme inhibition as visualized with the reservoir model: relationships between I50 and inhibition constant(s) of an enzyme inhibitor.
Computers in biology and medicine 1994;24(2):129-44.
1994: María A Günther Sillero; M Del Valle; E Zaera; P Michelena; Antonio G García; Antonio Sillero
Diadenosine 5',5"-P1,P4-tetraphosphate (Ap4A), ATP and catecholamine content in bovine adrenal medulla, chromaffin granules and chromaffin cells.
Biochimie 1994;76(5):404-9.
1994: Rui Fontes; J M Ribeiro; Antonio Sillero
A tridimensional representation of enzyme inhibition useful for diagnostic purposes.
Journal of enzyme inhibition 1994;8(2):73-85.
1993: Antonio Sillero; María A Günther Sillero; A Hernandorena
Effect of diets containing adenosine, guanosine, inosine or xanthosine on the nucleotide content of Artemia. Influence of mycophenolic acid.
Cell biochemistry and function 1993;11(3):193-200.
1993: B Ortiz; Antonio Sillero; María A Günther Sillero
Specific synthesis of adenosine(5')tetraphospho(5')nucleoside and adenosine(5')oligophospho(5')adenosine (n > 4) catalyzed by firefly luciferase.
European journal of biochemistry / FEBS 1993;212(1):263-70.
1992: C J Castillo; Maria Moro; M Del Valle; Antonio Sillero; Antonio G García; María A Günther Sillero
Diadenosine tetraphosphate is co-released with ATP and catecholamines from bovine adrenal medulla.
Journal of neurochemistry 1992;59(2):723-32.
1991: María A Günther Sillero; Andrzej Guranowski; Antonio Sillero
Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase.
European journal of biochemistry / FEBS 1991;202(2):507-13.
1991: D Bernet; Rosa María Pinto; Antonio Sillero; José Carlos Cameselle
Location of dinucleoside triphosphatase in the matrix space of rat liver mitochondria.
FEBS letters 1991;283(2):286-8.
1991: J García-Agúndez; José Carlos Cameselle; María Jesús Costas; María A Günther Sillero; Antonio Sillero
Particulate diadenosine 5',5"'-P1,P3-triphosphate hydrolases in rat brain: two specific dinucleoside triphosphatases and two phosphodiesterase I-like hydrolases.
Biochimica et biophysica acta 1991;1073(2):402-9.
1991: J M Ribeiro; Antonio Sillero
A program to calculate the isoelectric point of macromolecules.
Computers in biology and medicine 1991;21(3):131-41.
1991: C Wasternack; María A Günther Sillero; Antonio Sillero
Diadenosine polyphosphates inhibit the action of ribonucleotide reductase on ADP.
Biochemistry international 1991;23(1):151-6.
1990: J M Ribeiro; A Ruiz; María A Günther Sillero; Antonio Sillero
Theoretical isoelectric points and electric charges of mutated human hemoglobin subunits.
Clinica chimica acta; international journal of clinical chemistry 1990;190(3):189-97.
1990: Andrzej Guranowski; María A Günther Sillero; Antonio Sillero
Firefly luciferase synthesizes P1,P4-bis(5'-adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphates.
FEBS letters 1990;271(1-2):215-8.
1990: J Meireles Ribeiro; A Ruiz; Antonio Sillero; María A Günther Sillero
A low Km nucleoside 3'(2'),5-bisphosphate 3'(2')-phosphohydrolase from rat liver.
Biochimie 1990;72(4):227-34.
1990: J M Ribeiro; D Juzgado; E Crespo; Antonio Sillero
Computer program for the reservoir model of metabolic crossroads.
Computers in biology and medicine 1990;20(1):35-46.
1990: J M Ribeiro; Antonio Sillero
An algorithm for the computer calculation of the coefficients of a polynomial that allows determination of isoelectric points of proteins and other macromolecules.
Computers in biology and medicine 1990;20(4):235-42.
1989: M García-Díaz; José Canales; María A Günther Sillero; Antonio Sillero; José Carlos Cameselle
Phosphoglycerate kinase from yeast synthesizes guanosine 5'-tetraphosphate.
Biochemistry international 1989;19(6):1253-64.
1989: A Ruiz; C Hurtado; J Meireles Ribeiro; Antonio Sillero; María A Günther Sillero
Hydrolysis of bis(5'-nucleosidyl) polyphosphates by Escherichia coli 5'-nucleotidase.
Journal of bacteriology 1989;171(12):6703-9.
1989: Antonio Sillero; João Meireles Ribeiro
Isoelectric points of proteins: theoretical determination.
Analytical biochemistry 1989;179(2):319-25.
1988: F Buitrago; José Canales; Antonio Sillero
Presence of diguanosine tri, tetra-, and pentaphosphates in commercial samples of GTP and guanosine 5'-tetraphosphate.
Analytical biochemistry 1988;171(2):389-92.
1988: F Buitrago; José Canales; María A Günther Sillero; Antonio Sillero
Occurrence of a novel nucleotide, Zpp5'A2'p, in rat liver extracts.
Biochemistry international 1988;16(1):175-84.
1987: José Canales; F Buitrago; María A Günther Sillero; Antonio Sillero
Rat liver contains a novel nucleotide, Ypp5'A2'p, related to adenosine 2',5'-bisphosphate.
Biochemistry international 1987;15(2):419-29.
1987: C Hurtado; A Ruíz; Antonio Sillero; María A Günther Sillero
Specific magnesium-dependent diadenosine 5',5'''-P1,P3-triphosphate pyrophosphohydrolase in Escherichia coli.
Journal of bacteriology 1987;169(4):1718-23.
1987: Rosa María Pinto; José Canales; A Faraldo; Antonio Sillero; María A Günther Sillero
Cytosol 5'-nucleotidase from Artemia embryos. Purification and properties.
Comparative biochemistry and physiology. B, Comparative biochemistry 1987;86(1):49-53.
1986: Rosa María Pinto; José Canales; María A Günther Sillero; Antonio Sillero
Diadenosine tetraphosphate activates cytosol 5'-nucleotidase.
Biochemical and biophysical research communications 1986;138(1):261-7.
1986: F Buitrago; José Canales; María A Günther Sillero; Antonio Sillero
Occurrence of adenosine 2',5'-bisphosphate in rat liver.
Biochimica et biophysica acta 1986;881(2):276-80.
1986: M J Costas; José Carlos Cameselle; Antonio Sillero
Mitochondrial location of rat liver dinucleoside triphosphatase.
The Journal of biological chemistry 1986;261(5):2064-7.
1985: María Jesús Costas; José Carlos Cameselle; María A Günther Sillero; Antonio Sillero
Occurrence of dinucleosidetriphosphatase in the cytosol and particulate fractions from rat liver.
The International journal of biochemistry 1985;17(8):903-9.
1984: A Fernández; María Jesús Costas; María A Günther Sillero; Antonio Sillero
Diadenosine tetraphosphate activates AMP deaminase from rat muscle.
Biochemical and biophysical research communications 1984;121(1):155-61.
1984: José Carlos Cameselle; M J Costas; María A Günther Sillero; Antonio Sillero
Two low Km hydrolytic activities on dinucleoside 5',5"'-P1,P4-tetraphosphates in rat liver. Characterization as the specific dinucleoside tetraphosphatase and a phosphodiesterase I-like enzyme.
The Journal of biological chemistry 1984;259(5):2879-85.
1984: A Fernández; José Canales; Rosa María Pinto; A Faraldo; María A Günther Sillero; Antonio Sillero
Adenosine deaminase isozymes in Artemia.
Comparative biochemistry and physiology. B, Comparative biochemistry 1984;78(2):481-4.
1984: María Jesús Costas; J M Montero; José Carlos Cameselle; María A Günther Sillero; Antonio Sillero
Dinucleosidetriphosphatase from rat brain.
The International journal of biochemistry 1984;16(7):757-62.
1983: Rosa María Pinto; A Faraldo; A Fernández; José Canales; Antonio Sillero; María A Günther Sillero
Adenylosuccinate lyase from Artemia embryos. Purification and properties.
The Journal of biological chemistry 1983;258(20):12513-9.
1983: M J Costas; José Carlos Cameselle; María A Günther Sillero; Antonio Sillero
Presence of cytidine 5'-tetraphosphate in commerical samples of cytidine 5'-triphosphate.
Analytical biochemistry 1983;134(2):455-8.
1983: José Carlos Cameselle; María Jesús Costas; María A Günther Sillero; Antonio Sillero
Dinucleosidetetraphosphatase inhibition by Zn(II).
Biochemical and biophysical research communications 1983;113(2):717-22.
1982: V P Grau; María A Günther Sillero; Antonio Sillero
Binding of P1,P4-bis(5'-guanosyl)tetraphosphate to brain.
The Journal of biological chemistry 1982;257(20):11848-51.
1982: José Carlos Cameselle; María Jesús Costas; María A Günther Sillero; Antonio Sillero
Bis-(5'-guanosyl) tetraphosphatase in rat tissues.
The Biochemical journal 1982;201(2):405-10.
1982: A Moreno; Carmen Dominguez Lobaton; María A Günther Sillero; Antonio Sillero
Dinucleosidetetraphosphatase from Ehrlich ascites tumour cells: inhibition by adenosine, guanosine and uridine 5'-tetraphosphates.
The International journal of biochemistry 1982;14(7):629-34.