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Jan Slot

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Elly van Donselaar; George Posthuma; Dagmar Zeuschner; Bruno M Humbel; Jan W Slot
Immunogold labeling of cryosections from high-pressure frozen cells.
Jan W Slot; Hans J Geuze
Cryosectioning and immunolabeling.
Harry F G Heijnen; Elly van Donselaar; Jan W Slot; Diana M Fries; Beatrice Blachard-Fillion; Roberto Hodara; Richard Lightfoot; Manuela Polydoro; Dave Spielberg; Leonor Thomson; Elizabeth A Regan; James D Crapo; Harry Ischiropoulos
Subcellular localization of tyrosine-nitrated proteins is dictated by reactive oxygen species generating enzymes and by proximity to nitric oxide synthase.

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All Publications

2007: Elly van Donselaar; George Posthuma; Dagmar Zeuschner; Bruno M Humbel; Jan W Slot
Immunogold labeling of cryosections from high-pressure frozen cells.
Traffic (Copenhagen, Denmark) 2007;8(5):471-85.
2007: Jan W Slot; Hans J Geuze
Cryosectioning and immunolabeling.
Nature protocols 2007;2(10):2480-91.
2006: Harry F G Heijnen; Elly van Donselaar; Jan W Slot; Diana M Fries; Beatrice Blachard-Fillion; Roberto Hodara; Richard Lightfoot; Manuela Polydoro; Dave Spielberg; Leonor Thomson; Elizabeth A Regan; James D Crapo; Harry Ischiropoulos
Subcellular localization of tyrosine-nitrated proteins is dictated by reactive oxygen species generating enzymes and by proximity to nitric oxide synthase.
Free radical biology & medicine 2006;40(11):1903-13.
2006: Dagmar Zeuschner; Willie J.C. Geerts; Elly van Donselaar; Bruno M Humbel; Jan W Slot; Abraham Koster; Judith Klumperman
Immuno-electron tomography of ER exit sites reveals the existence of free COPII-coated transport carriers.
Nature cell biology 2006;8(4):377-83.
2004: Harry F G Heijnen; Sandra Waaijenborg; James D Crapo; Russell P Bowler; Jan-Willem N Akkerman; Jan W Slot
Colocalization of eNOS and the catalytic subunit of PKA in endothelial cell junctions: a clue for regulated NO production.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004;52(10):1277-85.
2003: Jean-Luc Murk; Bruno M Humbel; Ulrike Ziese; Janice M Griffith; George Posthuma; Jan W Slot; Abraham Koster; Arie J Verkleij; Hans J Geuze; Monique J Kleijmeer
Endosomal compartmentalization in three dimensions: implications for membrane fusion.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(23):13332-7.
2003: Diana M Fries; Evgenia Paxinou; Marios Themistocleous; Eric Swanberg; Kathy K Griendling; Daniela Salvemini; Jan W Slot; Harry F G Heijnen; Stanley L Hazen; Harry Ischiropoulos
Expression of inducible nitric-oxide synthase and intracellular protein tyrosine nitration in vascular smooth muscle cells: role of reactive oxygen species.
The Journal of biological chemistry 2003;278(25):22901-7.
2003: Harry F G Heijnen; Marjolijn van Lier; Sandra Waaijenborg; Yoshiko Ohno-Iwashita; Abdul A Waheed; Mitsushi Inomata; Gertie Gorter; Wiebke Möbius; Jan-Willem N Akkerman; Jan W Slot
Concentration of rafts in platelet filopodia correlates with recruitment of c-Src and CD63 to these domains.
Journal of thrombosis and haemostasis : JTH 2003;1(6):1161-73.
2003: Wiebke Möbius; Elly van Donselaar; Yoshiko Ohno-Iwashita; Y Shimada; Harry F G Heijnen; Jan W Slot; Hans J Geuze
Recycling compartments and the internal vesicles of multivesicular bodies harbor most of the cholesterol found in the endocytic pathway.
Traffic (Copenhagen, Denmark) 2003;4(4):222-31.
2003: Richard Wubbolts; Rachel S Leckie; Peter T M Veenhuizen; Guenter Schwarzmann; Wiebke Möbius; Joerg Hoernschemeyer; Jan W Slot; Hans J Geuze; Willem Stoorvogel
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
The Journal of biological chemistry 2003;278(13):10963-72.
2002: Ann M De Mazière; Katja Muehlethaler; Elly van Donselaar; Suzanne Salvi; Jean Davoust; Jean-Charles Cerottini; Frédéric Lévy; Jan W Slot; Donata Rimoldi
The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins.
Traffic (Copenhagen, Denmark) 2002;3(9):678-93.
2002: Roland Govers; Peter van Der Sluijs; Elly van Donselaar; Jan W Slot; ton rabelink
Endothelial nitric oxide synthase and its negative regulator caveolin-1 localize to distinct perinuclear organelles.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2002;50(6):779-88.
2002: Wiebke Möbius; Yoshiko Ohno-Iwashita; Elly van Donselaar; Viola Oorschot; Yukiko Shimada; Toyoshi Fujimoto; Harry F G Heijnen; Hans J Geuze; Jan W Slot
Immunoelectron microscopic localization of cholesterol using biotinylated and non-cytolytic perfringolysin O.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2002;50(1):43-55.
2001: J A Martinez-Menárguez; R Prekeris; Viola Oorschot; Richard H Scheller; Jan W Slot; Hans J Geuze; Judith Klumperman
Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport.
The Journal of cell biology 2001;155(7):1213-24.
2001: Adrian Oprins; Catherine Rabouille; George Posthuma; Judith Klumperman; Hans J Geuze; Jan W Slot
The ER to Golgi interface is the major concentration site of secretory proteins in the exocrine pancreatic cell.
Traffic (Copenhagen, Denmark) 2001;2(11):831-8.
2001: Russell P Bowler; John Arcaroli; James D Crapo; A Ross; Jan W Slot; Edward Abraham
Extracellular superoxide dismutase attenuates lung injury after hemorrhage.
American journal of respiratory and critical care medicine 2001;164(2):290-4.
2001: Abdul A Waheed; Yukiko Shimada; Harry F G Heijnen; Megumi Nakamura; Mitsushi Inomata; Masami Hayashi; Shintaro Iwashita; Jan W Slot; Yoshiko Ohno-Iwashita
Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts).
Proceedings of the National Academy of Sciences of the United States of America 2001;98(9):4926-31.
2000: Georg Ramm; Jan W Slot; David E James; Willem Stoorvogel
Insulin recruits GLUT4 from specialized VAMP2-carrying vesicles as well as from the dynamic endosomal/trans-Golgi network in rat adipocytes.
Molecular biology of the cell 2000;11(12):4079-91.
2000: Daniela Malide; Georg Ramm; Samuel W Cushman; Jan W Slot
Immunoelectron microscopic evidence that GLUT4 translocation explains the stimulation of glucose transport in isolated rat white adipose cells.
Journal of cell science 2000;113 Pt 23():4203-10.
1999: J A Martínez-Menárguez; Hans J Geuze; Jan W Slot; Judith Klumperman
Vesicular tubular clusters between the ER and Golgi mediate concentration of soluble secretory proteins by exclusion from COPI-coated vesicles.
Cell 1999;98(1):81-90.
1998: Hein Sprong; B Kruithof; Richtje Leijendekker; Jan W Slot; Gerrit van Meer; Peter van Der Sluijs
UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum.
The Journal of biological chemistry 1998;273(40):25880-8.
1998: K Zen; Kathleen L Notarfrancesco; Viola Oorschot; Jan W Slot; Aron B Fisher; Henry Shuman
Generation and characterization of monoclonal antibodies to alveolar type II cell lamellar body membrane.
The American journal of physiology 1998;275(1 Pt 1):L172-83.
1998: Ye-Shih Ho; Renaud Vincent; M S Dey; Jan W Slot; James D Crapo
Transgenic models for the study of lung antioxidant defense: enhanced manganese-containing superoxide dismutase activity gives partial protection to B6C3 hybrid mice exposed to hyperoxia.
American journal of respiratory cell and molecular biology 1998;18(4):538-47.
1997: BL Weller; James D Crapo; Jan W Slot; G Posthuma; Charles G Plopper; Kent E Pinkerton
Site- and cell-specific alteration of lung copper/zinc and manganese superoxide dismutases by chronic ozone exposure.
American journal of respiratory cell and molecular biology 1997;17(5):552-60.
1997: Harry F G Heijnen; Viola Oorschot; Jan J Sixma; Jan W Slot; David E James
Thrombin stimulates glucose transport in human platelets via the translocation of the glucose transporter GLUT-3 from alpha-granules to the cell surface.
The Journal of cell biology 1997;138(2):323-30.
1997: Jan W Slot; G Garruti; S Martin; Viola Oorschot; George Posthuma; Edward Kraegen; R Laybutt; G Thibault; David E James
Glucose transporter (GLUT-4) is targeted to secretory granules in rat atrial cardiomyocytes.
The Journal of cell biology 1997;137(6):1243-54.
1997: W Liou; Hans J Geuze; Math J H Geelen; Jan W Slot
The autophagic and endocytic pathways converge at the nascent autophagic vacuoles.
The Journal of cell biology 1997;136(1):61-70.
1996: W Liou; Hans J Geuze; Jan W Slot
Improving structural integrity of cryosections for immunogold labeling.
Histochemistry and cell biology 1996;106(1):41-58.
1995: Monica Fengsrud; N Roos; T Berg; W Liou; Jan W Slot; Per O Seglen
Ultrastructural and immunocytochemical characterization of autophagic vacuoles in isolated hepatocytes: effects of vinblastine and asparagine on vacuole distributions.
Experimental cell research 1995;221(2):504-19.
1994: Jaap J van Hellemond; Jan W Slot; Math J H Geelen; Lambert M G van Golde; P S Vermeulen
Ultrastructural localization of CTP:phosphoethanolamine cytidylyltransferase in rat liver.
The Journal of biological chemistry 1994;269(22):15415-8.
1994: Ger J Strous; Peter van Kerkhof; C Verheijen; John Rossen; W Liou; Jan W Slot; C A Roelen; Alan L Schwartz
Expression of functional growth hormone receptor in a mouse L cell line infected with recombinant vaccinia virus.
Experimental cell research 1994;211(2):353-9.
1994: A Oprins; Hans J Geuze; Jan W Slot
Cryosubstitution dehydration of aldehyde-fixed tissue: a favorable approach to quantitative immunocytochemistry.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1994;42(4):497-503.
1993: A Oprins; Rainer Dr. Duden; Thomas E Kreis; Hans J Geuze; Jan W Slot
Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas.
The Journal of cell biology 1993;121(1):49-59.
1993: C Rabouille; Ger J Strous; J D Crapo; Hans J Geuze; Jan W Slot
The differential degradation of two cytosolic proteins as a tool to monitor autophagy in hepatocytes by immunocytochemistry.
The Journal of cell biology 1993;120(4):897-908.
1993: W Liou; L Y Chang; Hans J Geuze; Ger J Strous; J D Crapo; Jan W Slot
Distribution of CuZn superoxide dismutase in rat liver.
Free radical biology & medicine 1993;14(2):201-7.
1992: Monique J Kleijmeer; A Kelly; Hans J Geuze; Jan W Slot; A Townsend; J Trowsdale
Location of MHC-encoded transporters in the endoplasmic reticulum and cis-Golgi.
Nature 1992;357(6376):342-4.
1991: I L van Genderen; Gerrit van Meer; Jan W Slot; Hans J Geuze; WF Voorhout
Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution.
The Journal of cell biology 1991;115(4):1009-19.
1991: Jan W Slot; Hans J Geuze; S Gigengack; David E James; G E Lienhard
Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(17):7815-9.
1991: Peter J Peters; Jannie Borst; Viola Oorschot; M Fukuda; O Krähenbühl; J Tschopp; Jan W Slot; Hans J Geuze
Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes.
The Journal of experimental medicine 1991;173(5):1099-109.
1991: Jan W Slot; Hans J Geuze; S Gigengack; G E Lienhard; David E James
Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat.
The Journal of cell biology 1991;113(1):123-35.
1990: Harry F G Heijnen; Joost A Koedam; H Sandberg; N H Beeser-Visser; Jan W Slot; Jan J Sixma
Characterization of human factor VIII and interaction with von Willebrand factor. An electron microscopic study.
European journal of biochemistry / FEBS 1990;194(2):491-8.
1990: Jan W Slot; R Moxley; Hans J Geuze; David E James
No evidence for expression of the insulin-regulatable glucose transporter in endothelial cells.
Nature 1990;346(6282):369-71.
1990: C V Harding; E R Unanue; Jan W Slot; A L Schwartz; Hans J Geuze
Functional and ultrastructural evidence for intracellular formation of major histocompatibility complex class II-peptide complexes during antigen processing.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(14):5553-7.
1989: Peter J Peters; Hans J Geuze; H A Van der Donk; Jan W Slot; J M Griffith; N J Stam; Hans Clevers; Jannie Borst
Molecules relevant for T cell-target cell interaction are present in cytolytic granules of human T lymphocytes.
European journal of immunology 1989;19(8):1469-75.
1989: Jan W Slot; George Posthuma; L Y Chang; J D Crapo; Hans J Geuze
Quantitative aspects of immunogold labeling in embedded and in nonembedded sections.
The American journal of anatomy 1989;185(2-3):271-81.
1988: J E Bleekemolen; M Stein; K von Figura; Jan W Slot; Hans J Geuze
The two mannose 6-phosphate receptors have almost identical subcellular distributions in U937 monocytes.
European journal of cell biology 1988;47(2):366-72.
1988: Hans J Geuze; Willem Stoorvogel; Ger J Strous; Jan W Slot; J E Bleekemolen; I Mellman
Sorting of mannose 6-phosphate receptors and lysosomal membrane proteins in endocytic vesicles.
The Journal of cell biology 1988;107(6 Pt 2):2491-501.
1988: George Posthuma; Jan W Slot; T Veenendaal; Hans J Geuze
Immunogold determination of amylase concentrations in pancreatic subcellular compartments.
European journal of cell biology 1988;46(2):327-35.
1988: J Blok; E M Gibbs; G E Lienhard; Jan W Slot; Hans J Geuze
Insulin-induced translocation of glucose transporters from post-Golgi compartments to the plasma membrane of 3T3-L1 adipocytes.
The Journal of cell biology 1988;106(1):69-76.
1987: George Posthuma; Jan W Slot; Hans J Geuze
Usefulness of the immunogold technique in quantitation of a soluble protein in ultra-thin sections.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1987;35(4):405-10.
1986: George Posthuma; Jan W Slot; Hans J Geuze
A quantitative immuno-electronmicroscopic study of amylase and chymotrypsinogen in peri- and tele-insular cells of the rat exocrine pancreas.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1986;34(2):203-7.
1986: Hans J Geuze; H A van der Donk; C F Simmons; Jan W Slot; Ger J Strous; Alan L Schwartz
Receptor-mediated endocytosis in liver parenchymal cells.
International review of experimental pathology 1986;29():113-71.
1985: Ger J Strous; Peter van Kerkhof; R Willemsen; Jan W Slot; Hans J Geuze
Effect of monensin on the metabolism, localization, and biosynthesis of N- and O-linked oligosaccharides of galactosyltransferase.
European journal of cell biology 1985;36(2):256-62.
1984: George Posthuma; Jan W Slot; Hans J Geuze
Immunocytochemical assays of amylase and chymotrypsinogen in rat pancreas secretory granules. Efficacy of using immunogold-labeled ultrathin cryosections to estimate relative protein concentrations.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1984;32(10):1028-34.
1984: M J Schilstra; Jan W Slot; P H van der Meide; George Posthuma; A F Cremers; L Bosch
Immunocytochemical localization of the elongation factor Tu in E. coli cells.
FEBS letters 1984;165(2):175-9.
1983: Ger J Strous; R Willemsen; Peter van Kerkhof; Jan W Slot; Hans J Geuze; Harvey Lodish
Vesicular stomatitis virus glycoprotein, albumin, and transferrin are transported to the cell surface via the same Golgi vesicles.
The Journal of cell biology 1983;97(6):1815-22.