Mathias Sprinzl

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Humenik Martin; Huang Yiwei; Safronov Igor; Sprinzl Mathias
Simultaneous and site-directed incorporation of an ester linkage and an azide group into a polypeptide by in vitro translation.
Pöhlmann Christopher; Humenik Martin; Sprinzl Mathias
Detection of bacterial 16S rRNA using multivalent dendrimer-reporter enzyme conjugates.
Pöhlmann Christopher; Wang Yiran; Humenik Martin; Heidenreich Bernd; Gareis Manfred; Sprinzl Mathias
Rapid, specific and sensitive electrochemical detection of foodborne bacteria.

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All Publications

2009: Humenik Martin; Huang Yiwei; Safronov Igor; Sprinzl Mathias
Simultaneous and site-directed incorporation of an ester linkage and an azide group into a polypeptide by in vitro translation.
Organic & biomolecular chemistry 2009;7(20):4218-24.
2009: Pöhlmann Christopher; Humenik Martin; Sprinzl Mathias
Detection of bacterial 16S rRNA using multivalent dendrimer-reporter enzyme conjugates.
Biosensors & bioelectronics 2009;24(11):3383-6.
2009: Pöhlmann Christopher; Wang Yiran; Humenik Martin; Heidenreich Bernd; Gareis Manfred; Sprinzl Mathias
Rapid, specific and sensitive electrochemical detection of foodborne bacteria.
Biosensors & bioelectronics 2009;24(9):2766-71.
2009: Sprinzl Mathias
Correction of errors during protein synthesis.
Angewandte Chemie (International ed. in English) 2009;48(21):3738-9.
2008: Humenik Martin; Pöhlmann Christopher; Wang Yiran; Sprinzl Mathias
Enhancement of electrochemical signal on gold electrodes by polyvalent esterase-dendrimer clusters.
Bioconjugate chemistry 2008;19(12):2456-61.
2008: Tóth Kamil; Sedlák Erik; Sprinzl Mathias; Zoldák Gabriel
Flexibility and enzyme activity of NADH oxidase from Thermus thermophilus in the presence of monovalent cations of Hofmeister series.
Biochimica et biophysica acta 2008;1784(5):789-95.
2008: Zoldák Gabriel; Sedlák Erik; Wolfrum Alexandra; Musatov Andrej; Fedunová Diana; Szkaradkiewicz Karol; Sprinzl Mathias
Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains.
Biochemistry 2008;47(17):4992-5005.
2008: Minarik Antonín; Humenik Martin; Li Sheng; Huang Yiwei; Krausch Georg; Sprinzl Mathias
Ligand-directed immobilization of proteins through an esterase 2 fusion tag studied by atomic force microscopy.
Chembiochem : a European journal of chemical biology 2008;9(1):124-30.
2008: Koch Miriam; Huang Yiwei; Sprinzl Mathias
Peptide-bond synthesis on the ribosome: no free vicinal hydroxy group required on the terminal ribose residue of peptidyl-tRNA.
Angewandte Chemie (International ed. in English) 2008;47(38):7242-5.
2007: Humenik Martin; Huang Yiwei; Wang Yiran; Sprinzl Mathias
C-terminal incorporation of bio-orthogonal azide groups into a protein and preparation of protein-oligodeoxynucleotide conjugates by Cu'-catalyzed cycloaddition.
Chembiochem : a European journal of chemical biology 2007;8(10):1103-6.
2007: Huang Yiwei; Humenik Martin; Sprinzl Mathias
Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides.
Protein expression and purification 2007;54(1):94-100.
2007: Wang Yiran; Stanzel Manfred; Gumbrecht Walter; Humenik Martin; Sprinzl Mathias
Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization.
Biosensors & bioelectronics 2007;22(8):1798-806.
2007: Zoldák Gabriel; Redecke Lars; Svergun Dmitri I; Konarev Peter V; Voertler C Stefan; Dobbek Holger; Sedlák Erik; Sprinzl Mathias
Release factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies.
Nucleic acids research 2007;35(4):1343-53.
2006: Li Sheng; Sprinzl Mathias
Interaction of immobilized human exportin-t with calf liver tRNA.
RNA biology 2006;3(4):145-9.
2006: Sprinzl Mathias
Chemistry of aminoacylation and peptide bond formation on the 3'terminus of tRNA.
Journal of biosciences 2006;31(4):489-96.
2006: Zoldák Gabriel; Sedlák Erik; Valusová Eva; Wolfrum Alexandra; Marek Jozef; Antalík Marián; Sprinzl Mathias
Irreversible thermal denaturation of elongation factor Ts from Thermus thermophilus effect of the residual structure and intermonomer disulfide bond.
Biochimica et biophysica acta 2006;1764(7):1277-85.
2006: Agafonov Dmitry E; Rabe Kersten S; Grote Michael; Voertler C Stefan; Sprinzl Mathias
C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1.
Chembiochem : a European journal of chemical biology 2006;7(2):330-6.
2006: Stupák Marek; Zoldák Gabriel; Musatov Andrej; Sprinzl Mathias; Sedlák Erik
Unusual effect of salts on the homodimeric structure of NADH oxidase from Thermus thermophilus in acidic pH.
Biochimica et biophysica acta 2006;1764(1):129-37.
2006: Sprinzl M; Milovnikova M; Voertler C S
RNA aptamers directed against oligosaccharides.
Handbook of experimental pharmacology 2006;(173):327-40.
2005: Sprinzl Mathias; Voertler C Stefan
Allosteric control of mRNA decoding.
Chembiochem : a European journal of chemical biology 2005;6(12):2143-5.
2005: Zoldák G; Musatov A; Stupák M; Sprinzl M; Sedlák E
pH-induced changes in activity and conformation of NADH oxidase from Thermus thermophilus.
General physiology and biophysics 2005;24(3):279-98.
2005: Agafonov Dmitry E; Huang Yiwei; Grote Michael; Sprinzl Mathias
Efficient suppression of the amber codon in E. coli in vitro translation system.
FEBS letters 2005;579(10):2156-60.
2005: Agafonov Dmitry E; Rabe Kersten S; Grote Michael; Huang Yiwei; Sprinzl Mathias
The esterase from Alicyclobacillus acidocaldarius as a reporter enzyme and affinity tag for protein biosynthesis.
FEBS letters 2005;579(10):2082-6.
2005: Sprinzl Mathias; Vassilenko Konstantin S
Compilation of tRNA sequences and sequences of tRNA genes.
Nucleic acids research 2005;33(Database issue):D139-40.
2004: Hartmann Roland K; Mörl Mario; Sprinzl Mathias
The tRNA world.
RNA (New York, N.Y.) 2004;10(3):344-9.
2004: Zoldák Gabriel; Sprinzl Mathias; Sedlák Erik
Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions.
European journal of biochemistry / FEBS 2004;271(1):48-57.
2003: Zoldák Gabriel; Sut'ák Róbert; Antalík Marián; Sprinzl Mathias; Sedlák Erik
Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus.
European journal of biochemistry / FEBS 2003;270(24):4887-97.
2002: Sedlák Erik; Zoldák Gabriel; Antalík Marián; Sprinzl Mathias
Thermodynamic properties of nucleotide-free EF-Tu from Thermus thermophilus in the presence of low-molecular weight effectors of its GTPase activity.
Biochimica et biophysica acta 2002;1597(1):22-7.
2002: Sedlák Erik; Sprinzl Mathias; Grillenbeck Norbert; Antalík Marián
Microcalorimetric study of elongation factor Tu from Thermus thermophilus in nucleotide-free, GDP and GTP forms and in the presence of elongation factor Ts.
Biochimica et biophysica acta 2002;1596(2):357-65.
2002: Szkaradkiewicz Karol; Nanninga Matthias; Nesper-Brock Martina; Gerrits Michael; Erdmann Volker A; Sprinzl Mathias
RNA aptamers directed against release factor 1 from Thermus thermophilus.
FEBS letters 2002;514(1):90-5.
2001: Schlosser A; Nawrot B; Grillenbeck N; Sprinzl M
Fluorescence-monitored conformational change on the 3'-end of tRNA upon aminoacylation.
Journal of biomolecular structure & dynamics 2001;19(2):285-91.
2001: Sedlák E; Valusová E; Nesper-Brock M; Antalík M; Sprinzl M
Effect of the central disulfide bond on the unfolding behavior of elongation factor Ts homodimer from Thermus thermophilus.
Biochemistry 2001;40(32):9579-86.
2001: Valusová E; Sedlák E; Antalík M; Nock S; Sprinzl M
Effect of N-domain on the stability of elongation factor Ts from Thermus thermophilus.
Biochimica et biophysica acta 2001;1547(1):117-26.
2000: Szkaradkiewicz K; Zuleeg T; Limmer S; Sprinzl M
Interaction of fMet-tRNAfMet and fMet-AMP with the C-terminal domain of Thermus thermophilus translation initiation factor 2.
European journal of biochemistry / FEBS 2000;267(13):4290-9.
2000: Sprinzl M; Brock S; Huang Y; Milovnik P; Nanninga M; Nesper-Brock M; Rütthard H; Szkaradkiewicz K
Regulation of GTPases in the bacterial translation machinery.
Biological chemistry 2000;381(5-6):367-75.
1998: Kirchner R; Vogtherr M; Limmer S; Sprinzl M
Secondary structure dimorphism and interconversion between hairpin and duplex form of oligoribonucleotides.
Antisense & nucleic acid drug development 1998;8(6):507-16.
1998: Brock S; Szkaradkiewicz K; Sprinzl M
Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors.
Molecular microbiology 1998;29(2):409-17.
1998: Nesper M; Nock S; Sedlák E; Antalík M; Podhradský D; Sprinzl M
Dimers of Thermus thermophilus elongation factor Ts are required for its function as a nucleotide exchange factor of elongation factor Tu.
European journal of biochemistry / FEBS 1998;255(1):81-6.
1998: Hornung V; Hofmann H P; Sprinzl M
In vitro selected RNA molecules that bind to elongation factor Tu.
Biochemistry 1998;37(20):7260-7.
1998: Nawrot B; Sprinzl M
Aminoacyl-tRNA analogues; synthesis, purification and properties of 3'-anthraniloyl oligoribonucleotides.
Nucleosides & nucleotides 1998;17(4):815-29.
1998: Op De Bekke A; Kiefmann M; Kremerskothen J; Vornlocher H P; Sprinzl M; Brosius J
The 10Sa RNA gene of Thermus thermophilus.
DNA sequence : the journal of DNA sequencing and mapping 1998;9(1):31-5.
1998: Sprinzl M; Horn C; Brown M; Ioudovitch A; Steinberg S
Compilation of tRNA sequences and sequences of tRNA genes.
Nucleic acids research 1998;26(1):148-53.
1997: Hofmann H P; Limmer S; Hornung V; Sprinzl M
Ni2+-binding RNA motifs with an asymmetric purine-rich internal loop and a G-A base pair.
RNA (New York, N.Y.) 1997;3(11):1289-300.
1997: Nawrot B; Hillenbrand R; Limmer S; Grillenbeck N; Sprinzl M
Interaction of N-tosyl-L-phenylalanylchloromethane with Thermus thermophilus elongation factor Tu.
European journal of biochemistry / FEBS 1997;247(1):59-65.
1997: Vornlocher H P; Kreutzer R; Sprinzl M
Organization of the Thermus thermophilus nusA/infB operon and overexpression of the infB gene in Escherichia coli.
Biochimie 1997;79(4):195-203.
1997: Nawrot B; Milius W; Ejchart A; Limmer S; Sprinzl M
The structure of 3'-O-anthraniloyladenosine, an analogue of the 3'-end of aminoacyl-tRNA.
Nucleic acids research 1997;25(5):948-54.
1997: Vornlocher H P; Scheible W R; Faulhammer H G; Sprinzl M
Identification and purification of translation initiation factor 2 (IF2) from Thermus thermophilus.
European journal of biochemistry / FEBS 1997;243(1-2):66-71.
1996: Jiang Y; Nock S; Nesper M; Sprinzl M; Sigler P B
Structure and importance of the dimerization domain in elongation factor Ts from Thermus thermophilus.
Biochemistry 1996;35(32):10269-78.
1996: Zeidler W; Schirmer N K; Egle C; Ribeiro S; Kreutzer R; Sprinzl M
Limited proteolysis and amino acid replacements in the effector region of Thermus thermophilus elongation factor Tu.
European journal of biochemistry / FEBS 1996;239(2):265-71.
1996: Limmer S; Reif B; Ott G; Arnold L; Sprinzl M
NMR evidence for helix geometry modifications by a G-U wobble base pair in the acceptor arm of E. coli tRNA(Ala).
FEBS letters 1996;385(1-2):15-20.
1996: Blank J; Nock S; Kreutzer R; Sprinzl M
Elongation factor Ts from Thermus thermophilus-- overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu.
European journal of biochemistry / FEBS 1996;236(1):222-7.
1996: Sprinzl M; Steegborn C; Hübel F; Steinberg S
Compilation of tRNA sequences and sequences of tRNA genes.
Nucleic acids research 1996;24(1):68-72.
1995: Hecht H J; Erdmann H; Park H J; Sprinzl M; Schmid R D
Crystal structure of NADH oxidase from Thermus thermophilus.
Nature structural biology 1995;2(12):1109-14.
1995: Ahmadian M R; Kreutzer R; Blechschmidt B; Sprinzl M
Site-directed mutagenesis of Thermus thermophilus EF-Tu: the substitution of threonine-62 by serine or alanine.
FEBS letters 1995;377(2):253-7.
1995: Nock S; Grillenbeck N; Ahmadian M R; Ribeiro S; Kreutzer R; Sprinzl M
Properties of isolated domains of the elongation factor Tu from Thermus thermophilus HB8.
European journal of biochemistry / FEBS 1995;234(1):132-9.
1995: Blank J; Grillenbeck N W; Kreutzer R; Sprinzl M
Overexpression and purification of Thermus thermophilus elongation factors G, Tu, and Ts from Escherichia coli.
Protein expression and purification 1995;6(5):637-45.
1995: Wagner A; Simon I; Sprinzl M; Goody R S
Interaction of guanosine nucleotides and their analogs with elongation factor Tu from Thermus thermophilus.
Biochemistry 1995;34(39):12535-42.
1995: Ribeiro S; Nock S; Sprinzl M
Purification of aminoacyl-tRNA by affinity chromatography on immobilized Thermus thermophilus EF-Tu.GTP.
Analytical biochemistry 1995;228(2):330-5.
1995: Zeidler W; Egle C; Ribeiro S; Wagner A; Katunin V; Kreutzer R; Rodnina M; Wintermeyer W; Sprinzl M
Site-directed mutagenesis of Thermus thermophilus elongation factor Tu. Replacement of His85, Asp81 and Arg300.
European journal of biochemistry / FEBS 1995;229(3):596-604.
1994: Sprinzl M
Elongation factor Tu: a regulatory GTPase with an integrated effector.
Trends in biochemical sciences 1994;19(6):245-50.
1994: Förster C; Limmer S; Zeidler W; Sprinzl M
Effector region of the translation elongation factor EF-Tu.GTP complex stabilizes an orthoester acid intermediate structure of aminoacyl-tRNA in a ternary complex.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(10):4254-7.
1994: Rudinger J; Blechschmidt B; Ribeiro S; Sprinzl M
Minimalist aminoacylated RNAs as efficient substrates for elongation factor Tu.
Biochemistry 1994;33(19):5682-8.
1994: Blechschmidt B; Shirokov V; Sprinzl M
Undecagold cluster modified tRNA(Phe) from Escherichia coli and its activity in the protein elongation cycle.
European journal of biochemistry / FEBS 1994;219(1-2):65-71.
1994: Stanzel M; Schön A; Sprinzl M
Discrimination against misacylated tRNA by chloroplast elongation factor Tu.
European journal of biochemistry / FEBS 1994;219(1-2):435-9.
1993: Förster C; Chakraburtty K; Sprinzl M
Discrimination between initiation and elongation of protein biosynthesis in yeast: identity assured by a nucleotide modification in the initiator tRNA.
Nucleic acids research 1993;21(24):5679-83.
1993: Berchtold H; Reshetnikova L; Reiser C O; Schirmer N K; Sprinzl M; Hilgenfeld R
Crystal structure of active elongation factor Tu reveals major domain rearrangements.
Nature 1993;365(6442):126-32.
1993: Löw A; Sprinzl M; Faulhammer H G
Affinity labeling of c-H-ras p21 consensus elements with periodate-oxidized GDP and GTP.
European journal of biochemistry / FEBS 1993;215(2):473-9.
1993: Steinberg S; Misch A; Sprinzl M
Compilation of tRNA sequences and sequences of tRNA genes.
Nucleic acids research 1993;21(13):3011-5.
1993: Limmer S; Hofmann H P; Ott G; Sprinzl M
The 3'-terminal end (NCCA) of tRNA determines the structure and stability of the aminoacyl acceptor stem.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(13):6199-202.
1993: Erdmann H; Hecht H J; Park H J; Sprinzl M; Schomburg D; Schmid R D
Crystallization and preliminary X-ray diffraction studies of a NADH oxidase from Thermus thermophilus HB8.
Journal of molecular biology 1993;230(3):1086-8.
1993: Löw A; Sprinzl M; Limmer S
Nucleotide binding and GTP hydrolysis by the 21-kDa product of the c-H-ras gene as monitored by proton-NMR spectroscopy.
European journal of biochemistry / FEBS 1993;213(2):781-8.
1993: Zeidler W; Kreutzer R; Sprinzl M
Participation of the overproduced elongation factor Tu from Thermus thermophilus in protein biosynthesis of Escherichia coli.
FEBS letters 1993;319(1-2):185-8.
1993: Park H J; Kreutzer R; Reiser C O; Sprinzl M
Molecular cloning and nucleotide sequence of the gene encoding a H2O2-forming NADH oxidase from the extreme thermophilic Thermus thermophilus HB8 and its expression in Escherichia coli.
European journal of biochemistry / FEBS 1993;211(3):909.
1993: Blechschmidt B; Jahn W; Hainfeld J F; Sprinzl M; Boublik M
Visualization of a ternary complex of the Escherichia coli Phe-tRNA(Phe) and Tu.GTP from Thermus thermophilus by scanning transmission electron microscopy.
Journal of structural biology 1993;110(1):84-9.
1993: Förster C; Limmer S; Ribeiro S; Hilgenfeld R; Sprinzl M
Ternary complex between elongation factor Tu.GTP and Phe-tRNA(Phe).
Biochimie 1993;75(12):1159-66.
1992: Kreutzer R; Kruft V; Bobkova E V; Lavrik O I; Sprinzl M
Structure of the phenylalanyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli.
Nucleic acids research 1992;20(16):4173-8.
1992: Löw A; Faulhammer H G; Sprinzl M
Affinity labeling of GTP-binding proteins in cellular extracts.
FEBS letters 1992;303(1):64-8.
1992: Park H J; Reiser C O; Kondruweit S; Erdmann H; Schmid R D; Sprinzl M
Purification and characterization of a NADH oxidase from the thermophile Thermus thermophilus HB8.
European journal of biochemistry / FEBS 1992;205(3):881-5.
1992: Park H J; Kreutzer R; Reiser C O; Sprinzl M
Molecular cloning and nucleotide sequence of the gene encoding a H2O2-forming NADH oxidase from the extreme thermophilic Thermus thermophilus HB8 and its expression in Escherichia coli.
European journal of biochemistry / FEBS 1992;205(3):875-9.
1992: Limmer S; Reiser C O; Schirmer N K; Grillenbeck N W; Sprinzl M
Nucleotide binding and GTP hydrolysis by elongation factor Tu from Thermus thermophilus as monitored by proton NMR.
Biochemistry 1992;31(11):2970-7.
1992: Grawunder U; Schön A; Sprinzl M
Sequence and base modifications of two phenylalanine-tRNAs from Thermus thermophilus HB8.
Nucleic acids research 1992;20(1):137.
1991: Reshetnikova L S; Reiser C O; Schirmer N K; Berchtold H; Storm R; Hilgenfeld R; Sprinzl M
Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to high resolution.
Journal of molecular biology 1991;221(2):375-7.
1991: Schirmer N K; Reiser C O; Sprinzl M
Effect of Thermus thermophilus elongation factor Ts on the conformation of elongation factor Tu.
European journal of biochemistry / FEBS 1991;200(2):295-300.
1991: Ahmadian M R; Kreutzer R; Sprinzl M
Overproduction of the Thermus thermophilus elongation factor Tu in Escherichia coli.
Biochimie 1991;73(7-8):1037-43.
1991: Sprinzl M; Dank N; Nock S; Schön A
Compilation of tRNA sequences and sequences of tRNA genes.
Nucleic acids research 1991;19 Suppl():2127-71.
1990: Peter M E; Reiser C O; Schirmer N K; Kiefhaber T; Ott G; Grillenbeck N W; Sprinzl M
Interaction of the isolated domain II/III of Thermus thermophilus elongation factor Tu with the nucleotide exchange factor EF-Ts.
Nucleic acids research 1990;18(23):6889-93.
1990: Kiesewetter S; Ott G; Sprinzl M
The role of modified purine 64 in initiator/elongator discrimination of tRNA(iMet) from yeast and wheat germ.
Nucleic acids research 1990;18(16):4677-82.
1990: Ott G; Schiesswohl M; Kiesewetter S; Förster C; Arnold L; Erdmann V A; Sprinzl M
Ternary complexes of Escherichia coli aminoacyl-tRNAs with the elongation factor Tu and GTP: thermodynamic and structural studies.
Biochimica et biophysica acta 1990;1050(1-3):222-5.
1990: Weisshaar M; Ahmadian R; Sprinzl M; Satoh M; Kushiro A; Tomita K
Sequences of four tRNA genes adjacent to the tuf2 gene of Thermus thermophilus.
Nucleic acids research 1990;18(7):1902.
1990: Peter M E; Schirmer N K; Reiser C O; Sprinzl M
Mapping the effector region in Thermus thermophilus elongation factor Tu.
Biochemistry 1990;29(11):2876-84.
1990: Förster C; Ott G; Forchhammer K; Sprinzl M
Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu from E.coli.
Nucleic acids research 1990;18(3):487-91.
1990: Ott G; Jonák J; Abrahams I P; Sprinzl M
The influence of different modifications of elongation factor Tu from Escherichia coli on ternary complex formation investigated by fluorescence spectroscopy.
Nucleic acids research 1990;18(3):437-41.
1989: Peter M E; Brockmöller J; Jonák J; Sprinzl M
Identification of the N-tosyl-L-phenylalanyl chloromethylketone modification site in Thermus thermophilus elongation factor Tu.
FEBS letters 1989;257(2):219-22.
1989: Ott G; Faulhammer H G; Sprinzl M
Interaction of elongation factor Tu from Escherichia coli with aminoacyl-tRNA carrying a fluorescent reporter group on the 3' terminus.
European journal of biochemistry / FEBS 1989;184(2):345-52.
1989: Schön A; Böck A; Ott G; Sprinzl M; Söll D
The selenocysteine-inserting opal suppressor serine tRNA from E. coli is highly unusual in structure and modification.
Nucleic acids research 1989;17(18):7159-65.
1988: Peter M E; Wittmann-Liebold B; Sprinzl M
Affinity labeling of the GDP/GTP binding site in Thermus thermophilus elongation factor Tu.
Biochemistry 1988;27(26):9132-9.
1987: Seidler L; Peter M; Meissner F; Sprinzl M
Sequence and identification of the nucleotide binding site for the elongation factor Tu from Thermus thermophilus HB8.
Nucleic acids research 1987;15(22):9263-77.

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