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James Spudich

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Mary Williard Elting; Jonas Korlach; Jason S Leith; Sabrina R Leslie; Michael J Levene; Christopher M J McFaul; L Stirling Churchman; Adam E Cohen; James A Spudich
Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).
Mary Williard Elting; James A Spudich
Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.
James A Spudich
One path to understanding energy transduction in biological systems.

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All Publications

2013: Mary Williard Elting; Jonas Korlach; Jason S Leith; Sabrina R Leslie; Michael J Levene; Christopher M J McFaul; L Stirling Churchman; Adam E Cohen; James A Spudich
Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).
Optics express 2013;21(1):1189-202.
2012: Mary Williard Elting; James A Spudich
Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.
Developmental cell 2012;23(6):1084-91.
2012: James A Spudich
One path to understanding energy transduction in biological systems.
Nature medicine 2012;18(10):1478-82.
2012: Peiying Chuan; Sivaraj Sivaramakrishnan; Euan Ashley; James A Spudich
Cell-intrinsic functional effects of the α-cardiac myosin Arg-403-Gln mutation in familial hypertrophic cardiomyopathy.
Biophysical journal 2012;102(12):2782-90.
2012: M Amanda Hartman; James A Spudich
The myosin superfamily at a glance.
Journal of cell science 2012;125(Pt 7):1627-32.
2012: L Stirling Churchman; James A Spudich
Single-molecule high-resolution colocalization of single probes.
Cold Spring Harbor protocols 2012;2012(2):242-5.
2012: L Stirling Churchman; James A Spudich
Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.
Cold Spring Harbor protocols 2012;2012(2):141-9.
2011: James A Spudich; Thomas J Purcell; Sarah E Rice; Ronald S Rock; Hans M Warrick
The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments.
Cold Spring Harbor protocols 2011;2011(11):1372-4.
2011: James A Spudich; Thomas J Purcell; Sarah E Rice; Ronald S Rock; Hans M Warrick
Attachment of anti-GFP antibodies to microspheres for optical trapping experiments.
Cold Spring Harbor protocols 2011;2011(11):1370-1.
2011: James A Spudich; Thomas J Purcell; Sarah E Rice; Ronald S Rock; Hans M Warrick
Optical traps to study properties of molecular motors.
Cold Spring Harbor protocols 2011;2011(11):1305-18.
2011: James A Spudich
Molecular motors: forty years of interdisciplinary research.
Molecular biology of the cell 2011;22(21):3936-9.
2011: Dina Finan; M Amanda Hartman; James A Spudich
Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(14):5566-71.
2011: Thomas J Purcell; Nariman Naber; Kathleen Franks-Skiba; Alexander R Dunn; Catherine C Eldred; Christopher L Berger; András Málnási-Csizmadia; James A Spudich; Douglas M Swank; Edward Pate; Roger Cooke
Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.
Journal of molecular biology 2011;407(1):79-91.
2011: Mary Williard Elting; Zev Bryant; Jung-Chi Liao; James A Spudich
Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI.
Biophysical journal 2011;100(2):430-9.
2011: Peiying Chuan; James A Spudich; Alexander R Dunn
Robust mechanosensing and tension generation by myosin VI.
Journal of molecular biology 2011;405(1):105-12.
2011: M Amanda Hartman; Sivaraj Sivaramakrishnan; Dina Finan; James A Spudich
Principles of unconventional myosin function and targeting.
Annual review of cell and developmental biology 2011;27():133-55.
2010: Ruth F Sommese; Sivaraj Sivaramakrishnan; Robert L Baldwin; James A Spudich
Helicity of short E-R/K peptides.
Protein science : a publication of the Protein Society 2010;19(10):2001-5.
2010: Kim I Mortensen; L Stirling Churchman; James A Spudich; Henrik Flyvbjerg
Optimized localization analysis for single-molecule tracking and super-resolution microscopy.
Nature methods 2010;7(5):377-81.
2010: Ashley R Dunn; Peiying Chuan; Zev Bryant; James A Spudich
Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(17):7746-50.
2010: James A Spudich; Sivaraj Sivaramakrishnan
Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis.
Nature reviews. Molecular cell biology 2010;11(2):128-37.
2010: Jongmin Sung; Sivaraj Sivaramakrishnan; Alexander R Dunn; James A Spudich
Single-molecule dual-beam optical trap analysis of protein structure and function.
Methods in enzymology 2010;475():321-75.
2009: Sivaraj Sivaramakrishnan; J Sung; M Ali; Sebastian Doniach; H Flyvbjerg; James A Spudich
Combining single-molecule optical trapping and small-angle x-ray scattering measurements to compute the persistence length of a protein ER/K alpha-helix.
Biophysical journal 2009;97(11):2993-9.
2009: Sivaraj Sivaramakrishnan; Euan Ashley; Leslie Leinwand; James A Spudich
Insights into human beta-cardiac myosin function from single molecule and single cell studies.
Journal of cardiovascular translational research 2009;2(4):426-40.
2009: Sivaraj Sivaramakrishnan; James A Spudich
Coupled myosin VI motors facilitate unidirectional movement on an F-actin network.
The Journal of cell biology 2009;187(1):53-60.
2009: Jung-Chi Liao; Mary Williard Elting; Scott L Delp; James A Spudich; Zev Bryant
Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity.
Journal of molecular biology 2009;392(4):862-7.
2008: James A Spudich
Dynamic organization of gene loci and transcription compartments in the cell nucleus.
Biophysical journal 2008;95(11):5003-4.
2008: Sivaraj Sivaramakrishnan; Benjamin J Spink; Adelene Y L Sim; Sebastian Doniach; James A Spudich
Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motif.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(36):13356-61.
2008: Benjamin J Spink; Sivaraj Sivaramakrishnan; Jan Lipfert; Sebastian Doniach; James A Spudich
Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI.
Nature structural & molecular biology 2008;15(6):591-7.
2008: James A Spudich
Molecular motors: a surprising twist in myosin VI translocation.
Current biology : CB 2008;18(2):R68-70.
2007: Susan Tang; Jung-Chi Liao; Alexander R Dunn; Russ B Altman; James A Spudich; Jeanette P Schmidt
Predicting allosteric communication in myosin via a pathway of conserved residues.
Journal of molecular biology 2007;373(5):1361-73.
2007: Qian Chen; Gandikota S Lakshmikanth; James A Spudich; Arturo De Lozanne
The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton.
Molecular biology of the cell 2007;18(9):3366-74.
2007: David Altman; Debanjan Goswami; Tama Hasson; James A Spudich; Satyajit Mayor
Precise positioning of myosin VI on endocytic vesicles in vivo.
PLoS biology 2007;5(8):e210.
2007: Nathan C Geething; James A Spudich
Identification of a minimal myosin Va binding site within an intrinsically unstructured domain of melanophilin.
The Journal of biological chemistry 2007;282(29):21518-28.
2007: Alexander R Dunn; James A Spudich
Dynamics of the unbound head during myosin V processive translocation.
Nature structural & molecular biology 2007;14(3):246-8.
2007: Jung-Chi Liao; James A Spudich; David Parker; Scott L Delp
Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathways.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(9):3171-6.
2007: Zev Bryant; David Altman; James A Spudich
The power stroke of myosin VI and the basis of reverse directionality.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(3):772-7.
2006: James A Spudich
Molecular motors take tension in stride.
Cell 2006;126(2):242-4.
2006: L Stirling Churchman; Henrik Flyvbjerg; James A Spudich
A non-Gaussian distribution quantifies distances measured with fluorescence localization techniques.
Biophysical journal 2006;90(2):668-71.
2006: Sara O Dean; James A Spudich
Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation.
PloS one 2006;1():e131.
2005: Thomas J Purcell; H Lee Sweeney; James A Spudich
A force-dependent state controls the coordination of processive myosin V.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(39):13873-8.
2005: Sara O Dean; Stephen L Rogers; Nico Stuurman; Ronald D Vale; James A Spudich
Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(38):13473-8.
2005: Ronald S Rock; Bhagavathi Ramamurthy; Alexander R Dunn; Sara Beccafico; Bhadresh R Rami; Carl Morris; Benjamin J Spink; Clara Franzini-Armstrong; James A Spudich; H Lee Sweeney
A flexible domain is essential for the large step size and processivity of myosin VI.
Molecular cell 2005;17(4):603-9.
2005: L Stirling Churchman; Zeynep Okten; Ronald S Rock; John F Dawson; James A Spudich
Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(5):1419-23.
2004: Gandikota S Lakshmikanth; Hans M Warrick; James A Spudich
A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(47):16519-24.
2004: James A Spudich
Two important polymers cross paths.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(45):15825-6.
2004: Daniel Hostetter; Sarah Rice; Sara Dean; David Altman; Peggy M McMahon; Shirley Sutton; Ashutosh Tripathy; James A Spudich
Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod.
PLoS biology 2004;2(11):e356.
2004: Zeynep Okten; L Stirling Churchman; Ronald S Rock; James A Spudich
Myosin VI walks hand-over-hand along actin.
Nature structural & molecular biology 2004;11(9):884-7.
2004: Douglas Robinson; James A Spudich
Mechanics and regulation of cytokinesis.
Current opinion in cell biology 2004;16(2):182-8.
2004: David Altman; H Lee Sweeney; James A Spudich
The mechanism of myosin VI translocation and its load-induced anchoring.
Cell 2004;116(5):737-49.
2003: John F Dawson; Elena P Sablin; James A Spudich; Robert J Fletterick
Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severing.
The Journal of biological chemistry 2003;278(2):1229-38.
2003: Sarah E Rice; Thomas J Purcell; James A Spudich
Building and using optical traps to study properties of molecular motors.
Methods in enzymology 2003;361():112-33.
2002: Thomas J Purcell; Carl Morris; James A Spudich; H Lee Sweeney
Role of the lever arm in the processive stepping of myosin V.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(22):14159-64.
2002: Elena P Sablin; John F Dawson; Margaret S VanLoock; James A Spudich; Edward H Egelman; Robert J Fletterick
How does ATP hydrolysis control actin's associations?
Proceedings of the National Academy of Sciences of the United States of America 2002;99(17):10945-7.
2002: Douglas Robinson; Stephani S Ocon; Ronald S Rock; James A Spudich
Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures.
The Journal of biological chemistry 2002;277(11):9088-95.
2002: Douglas Robinson; Guy Cavet; Hans M Warrick; James A Spudich
Quantitation of the distribution and flux of myosin-II during cytokinesis.
BMC cell biology 2002;3():4.
2002: James A Spudich; Ronald S Rock
A crossbridge too far.
Nature cell biology 2002;4(1):E8-10.
2001: Ronald S Rock; S E Rice; Amber L. Wells; Thomas J Purcell; James A Spudich; H Lee Sweeney
Myosin VI is a processive motor with a large step size.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(24):13655-9.
2001: William M Shih; James A Spudich
The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle.
The Journal of biological chemistry 2001;276(22):19491-4.
2001: James A Spudich
The myosin swinging cross-bridge model.
Nature reviews. Molecular cell biology 2001;2(5):387-92.
2001: CT Murphy; Ronald S Rock; James A Spudich
A myosin II mutation uncouples ATPase activity from motility and shortens step size.
Nature cell biology 2001;3(3):311-5.
2000: Ronald S Rock; Matthias Rief; Amit D Mehta; James A Spudich
In vitro assays of processive myosin motors.
Methods (San Diego, Calif.) 2000;22(4):373-81.
2000: William M Shih; Zygmunt Gryczynski; Joseph R Lakowicz; James A Spudich
A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin.
Cell 2000;102(5):683-94.
2000: Douglas Robinson; James A Spudich
Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant.
The Journal of cell biology 2000;150(4):823-38.
2000: Matthias Rief; Ronald S Rock; Amit D Mehta; Mark S Mooseker; Richard E Cheney; James A Spudich
Myosin-V stepping kinetics: a molecular model for processivity.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(17):9482-6.
2000: Douglas Robinson; James A Spudich
Towards a molecular understanding of cytokinesis.
Trends in cell biology 2000;10(6):228-37.
2000: CT Murphy; James A Spudich
Variable surface loops and myosin activity: accessories to a motor.
Journal of muscle research and cell motility 2000;21(2):139-51.
2000: S Nock; Wenchuan Liang; Hans M Warrick; James A Spudich
Mutational analysis of phosphorylation sites in the Dictyostelium myosin II tail: disruption of myosin function by a single charge change.
FEBS letters 2000;466(2-3):267-72.
1999: Wenchuan Liang; Hans M Warrick; James A Spudich
A structural model for phosphorylation control of Dictyostelium myosin II thick filament assembly.
The Journal of cell biology 1999;147(5):1039-48.
1999: Amit D Mehta; Ronald S Rock; Matthias Rief; James A Spudich; Mark S Mooseker; Richard E Cheney
Myosin-V is a processive actin-based motor.
Nature 1999;400(6744):590-3.
1999: Amit D Mehta; Matthias Rief; James A Spudich
Biomechanics, one molecule at a time.
The Journal of biological chemistry 1999;274(21):14517-20.
1999: CT Murphy; James A Spudich
The sequence of the myosin 50-20K loop affects Myosin's affinity for actin throughout the actin-myosin ATPase cycle and its maximum ATPase activity.
Biochemistry 1999;38(12):3785-92.
1999: H V Goodson; Hans M Warrick; James A Spudich
Specialized conservation of surface loops of myosin: evidence that loops are involved in determining functional characteristics.
Journal of molecular biology 1999;287(1):173-85.
1999: Amit D Mehta; Matthias Rief; James A Spudich; D A Smith; R M Simmons
Single-molecule biomechanics with optical methods.
Science (New York, N.Y.) 1999;283(5408):1689-95.
1998: JH Zang; James A Spudich
Myosin II localization during cytokinesis occurs by a mechanism that does not require its motor domain.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(23):13652-7.
1998: L A Silveira; J L Smith; J L Tan; James A Spudich
MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(22):13000-5.
1998: Wenchuan Liang; James A Spudich
Nucleotide-dependent conformational change near the fulcrum region in Dictyostelium myosin II.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(22):12844-7.
1998: A L Friedman; M A Geeves; D J Manstein; James A Spudich
Kinetic characterization of myosin head fragments with long-lived myosin.ATP states.
Biochemistry 1998;37(27):9679-87.
1998: Amit D Mehta; K A Pullen; James A Spudich
Single molecule biochemistry using optical tweezers.
FEBS letters 1998;430(1-2):23-7.
1998: SL Moores; James A Spudich
Conditional loss-of-myosin-II-function mutants reveal a position in the tail that is critical for filament nucleation.
Molecular cell 1998;1(7):1043-50.
1998: CT Murphy; James A Spudich
Dictyostelium myosin 25-50K loop substitutions specifically affect ADP release rates.
Biochemistry 1998;37(19):6738-44.
1998: Amit D Mehta; JT Finer; James A Spudich
Use of optical traps in single-molecule study of nonprocessive biological motors.
Methods in enzymology 1998;298():436-59.
1998: Amit D Mehta; JT Finer; James A Spudich
Reflections of a lucid dreamer: optical trap design considerations.
Methods in cell biology 1998;55():47-69.
1997: JH Zang; Guy Cavet; J H Sabry; P Wagner; SL Moores; James A Spudich
On the role of myosin-II in cytokinesis: division of Dictyostelium cells under adhesive and nonadhesive conditions.
Molecular biology of the cell 1997;8(12):2617-29.
1997: J H Sabry; SL Moores; S Ryan; JH Zang; James A Spudich
Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.
Molecular biology of the cell 1997;8(12):2605-15.
1997: S Nock; James A Spudich; P Wagner
Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces.
FEBS letters 1997;414(2):233-8.
1997: Amit D Mehta; JT Finer; James A Spudich
Detection of single-molecule interactions using correlated thermal diffusion.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(15):7927-31.
1997: K C Giese; James A Spudich
Phenotypically selected mutations in myosin's actin binding domain demonstrate intermolecular contacts important for motor function.
Biochemistry 1997;36(28):8465-73.
1997: P Wagner; S Nock; James A Spudich; W D Volkmuth; S Chu; R L Cicero; C P Wade; M R Linford; Christopher E D Chidsey
Bioreactive self-assembled monolayers on hydrogen-passivated Si(111) as a new class of atomically flat substrates for biological scanning probe microscopy.
Journal of structural biology 1997;119(2):189-201.
1996: J L Smith; L A Silveira; James A Spudich
Activation of Dictyostelium myosin light chain kinase A by phosphorylation of Thr166.
The EMBO journal 1996;15(22):6075-83.
1996: J L Smith; L A Silveira; James A Spudich
Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(22):12321-6.
1996: G H Nuckolls; N Osherov; W F Loomis; James A Spudich
The Dictyostelium dual-specificity kinase splA is essential for spore differentiation.
Development (Cambridge, England) 1996;122(10):3295-305.
1996: K M Ruppel; James A Spudich
Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft.
Molecular biology of the cell 1996;7(7):1123-36.
1996: B Patterson; James A Spudich
Cold-sensitive mutations of Dictyostelium myosin heavy chain highlight functional domains of the myosin motor.
Genetics 1996;143(2):801-10.
1996: H V Goodson; B L Anderson; Hans M Warrick; Liza A Pon; James A Spudich
Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton.
The Journal of cell biology 1996;133(6):1277-91.
1996: R M Simmons; J T Finer; S Chu; James A Spudich
Quantitative measurements of force and displacement using an optical trap.
Biophysical journal 1996;70(4):1813-22.
1996: S L Moores; J H Sabry; James A Spudich
Myosin dynamics in live Dictyostelium cells.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(1):443-6.
1996: K M Ruppel; James A Spudich
Structure-function analysis of the motor domain of myosin.
Annual review of cell and developmental biology 1996;12():543-73.
1995: B Patterson; James A Spudich
A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium.
Genetics 1995;140(2):505-15.
1995: J T Finer; Amit D Mehta; James A Spudich
Characterization of single actin-myosin interactions.
Biophysical journal 1995;68(4 Suppl):291S-296S; discussion 296S-297S.
1995: K M Ruppel; Michael Lorenz; James A Spudich
Myosin structure/function: a combined mutagenesis-crystallography approach.
Current opinion in structural biology 1995;5(2):181-6.
1995: K M Ruppel; James A Spudich
Myosin motor function: structural and mutagenic approaches.
Current opinion in cell biology 1995;7(1):89-93.
1995: James A Spudich; J Finer; B Simmons; K Ruppel; B Patterson; T Uyeda
Myosin structure and function.
Cold Spring Harbor symposia on quantitative biology 1995;60():783-91.
1994: James A Spudich
How molecular motors work.
Nature 1994;372(6506):515-8.
1994: R J Lee; Thomas T Egelhoff; James A Spudich
Molecular genetic truncation analysis of filament assembly and phosphorylation domains of Dictyostelium myosin heavy chain.
Journal of cell science 1994;107 ( Pt 10)():2875-86.
1994: Matthew L Springer; B Patterson; James A Spudich
Stage-specific requirement for myosin II during Dictyostelium development.
Development (Cambridge, England) 1994;120(9):2651-60.
1994: K M Ruppel; T Q Uyeda; James A Spudich
Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin.
The Journal of biological chemistry 1994;269(29):18773-80.
1994: T Q Uyeda; K M Ruppel; James A Spudich
Enzymatic activities correlate with chimaeric substitutions at the actin-binding face of myosin.
Nature 1994;368(6471):567-9.
1994: J T Finer; R M Simmons; James A Spudich
Single myosin molecule mechanics: piconewton forces and nanometre steps.
Nature 1994;368(6467):113-9.
1993: T Q Uyeda; James A Spudich
A functional recombinant myosin II lacking a regulatory light chain-binding site.
Science (New York, N.Y.) 1993;262(5141):1867-70.
1993: Thomas T Egelhoff; R J Lee; James A Spudich
Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo.
Cell 1993;75(2):363-71.
1993: M A Titus; Deborah Wessels; James A Spudich; David Soll
The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.
Molecular biology of the cell 1993;4(2):233-46.
1993: H V Goodson; James A Spudich
Molecular evolution of the myosin family: relationships derived from comparisons of amino acid sequences.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(2):659-63.
1993: Hans M Warrick; R M Simmons; J T Finer; T Q Uyeda; S Chu; James A Spudich
In vitro methods for measuring force and velocity of the actin-myosin interaction using purified proteins.
Methods in cell biology 1993;39():1-21.
1993: R M Simmons; J T Finer; Hans M Warrick; B Kralik; S Chu; James A Spudich
Force on single actin filaments in a motility assay measured with an optical trap.
Advances in experimental medicine and biology 1993;332():331-6; discussion 336-7.
1992: E W Kubalek; T Q Uyeda; James A Spudich
A Dictyostelium myosin II lacking a proximal 58-kDa portion of the tail is functional in vitro and in vivo.
Molecular biology of the cell 1992;3(12):1455-62.
1992: S Ravid; James A Spudich
Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(13):5877-81.
1992: S J Kron; David G Drubin; David Botstein; James A Spudich
Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(10):4466-70.
1992: J L Tan; S Ravid; James A Spudich
Control of nonmuscle myosins by phosphorylation.
Annual review of biochemistry 1992;61():721-59.
1991: B Patterson; K M Ruppel; James A Spudich
Molecular genetic approaches to the cytoskeleton in Dictyostelium.
Current opinion in genetics & development 1991;1(3):378-82.
1991: J L Tan; James A Spudich
Characterization and bacterial expression of the Dictyostelium myosin light chain kinase cDNA. Identification of an autoinhibitory domain.
The Journal of biological chemistry 1991;266(24):16044-9.
1991: T Q Uyeda; Hans M Warrick; S J Kron; James A Spudich
Quantized velocities at low myosin densities in an in vitro motility assay.
Nature 1991;352(6333):307-11.
1991: Thomas T Egelhoff; James A Spudich
Molecular genetics of cell migration: Dictyostelium as a model system.
Trends in genetics : TIG 1991;7(5):161-6.
1991: Thomas T Egelhoff; S S Brown; James A Spudich
Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo.
The Journal of cell biology 1991;112(4):677-88.
1991: S J Kron; Y Y Toyoshima; T Q Uyeda; James A Spudich
Assays for actin sliding movement over myosin-coated surfaces.
Methods in enzymology 1991;196():399-416.
1991: Thomas T Egelhoff; M A Titus; Dietmar J Manstein; K M Ruppel; James A Spudich
Molecular genetic tools for study of the cytoskeleton in Dictyostelium.
Methods in enzymology 1991;196():319-34.
1991: Dietmar J Manstein; K M Ruppel; L Kubalek; James A Spudich
Manipulation and expression of molecular motors in Dictyostelium discoideum.
Journal of cell science. Supplement 1991;14():63-5.
1991: S J Kron; T Q Uyeda; Hans M Warrick; James A Spudich
An approach to reconstituting motility of single myosin molecules.
Journal of cell science. Supplement 1991;14():129-33.
1990: T J O'Halloran; James A Spudich
Genetically engineered truncated myosin in Dictyostelium: the carboxyl-terminal regulatory domain is not required for the developmental cycle.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(20):8110-4.
1990: Y Y Toyoshima; S J Kron; James A Spudich
The myosin step size: measurement of the unit displacement per ATP hydrolyzed in an in vitro assay.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(18):7130-4.
1990: J L Tan; James A Spudich
Dictyostelium myosin light chain kinase. Purification and characterization.
The Journal of biological chemistry 1990;265(23):13818-24.
1990: T Q Uyeda; S J Kron; James A Spudich
Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin.
Journal of molecular biology 1990;214(3):699-710.
1990: D H Schwyter; S J Kron; Y Y Toyoshima; James A Spudich; Emil Reisler
Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin.
The Journal of cell biology 1990;111(2):465-70.
1990: J L Tan; James A Spudich
Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum.
Molecular and cellular biology 1990;10(7):3578-83.
1990: Thomas T Egelhoff; Dietmar J Manstein; James A Spudich
Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection.
Developmental biology 1990;137(2):359-67.
1990: M A Titus; Hans M Warrick; James A Spudich
Molecular genetics: a key to the cytoskeleton's closet.
Current opinion in cell biology 1990;2(1):116-20.
1990: T J O'Halloran; S Ravid; James A Spudich
Expression of Dictyostelium myosin tail segments in Escherichia coli: domains required for assembly and phosphorylation.
The Journal of cell biology 1990;110(1):63-70.
1990: K M Ruppel; Thomas T Egelhoff; James A Spudich
Purification of a functional recombinant myosin fragment from Dictyostelium discoideum.
Annals of the New York Academy of Sciences 1990;582():147-55.
1989: Dietmar J Manstein; K M Ruppel; James A Spudich
Expression and characterization of a functional myosin head fragment in Dictyostelium discoideum.
Science (New York, N.Y.) 1989;246(4930):656-8.
1989: M A Titus; Hans M Warrick; James A Spudich
Multiple actin-based motor genes in Dictyostelium.
Cell regulation 1989;1(1):55-63.
1989: James A Spudich
In pursuit of myosin function.
Cell regulation 1989;1(1):1-11.
1989: C Pasternak; James A Spudich; E L Elson
Capping of surface receptors and concomitant cortical tension are generated by conventional myosin.
Nature 1989;341(6242):549-51.
1989: Y Y Toyoshima; Chikashi Toyoshima; James A Spudich
Bidirectional movement of actin filaments along tracks of myosin heads.
Nature 1989;341(6238):154-6.
1989: S Ravid; James A Spudich
Myosin heavy chain kinase from developed Dictyostelium cells. Purification and characterization.
The Journal of biological chemistry 1989;264(25):15144-50.
1989: C Pasternak; P F Flicker; S Ravid; James A Spudich
Intermolecular versus intramolecular interactions of Dictyostelium myosin: possible regulation by heavy chain phosphorylation.
The Journal of cell biology 1989;109(1):203-10.
1989: Thomas T Egelhoff; S S Brown; Dietmar J Manstein; James A Spudich
Hygromycin resistance as a selectable marker in Dictyostelium discoideum.
Molecular and cellular biology 1989;9(5):1965-8.
1989: Dietmar J Manstein; M A Titus; A De Lozanne; James A Spudich
Gene replacement in Dictyostelium: generation of myosin null mutants.
The EMBO journal 1989;8(3):923-32.
1988: Hans M Warrick; James A Spudich
Codon preference in Dictyostelium discoideum.
Nucleic acids research 1988;16(14A):6617-35.
1987: A De Lozanne; C H Berlot; L A Leinwand; James A Spudich
Expression in Escherichia coli of a functional Dictyostelium myosin tail fragment.
The Journal of cell biology 1987;105(6 Pt 2):2999-3005.
1987: Y Y Toyoshima; S J Kron; Elizabeth McNally; K R Niebling; Chikashi Toyoshima; James A Spudich
Myosin subfragment-1 is sufficient to move actin filaments in vitro.
Nature 1987;328(6130):536-9.
1987: Hans M Warrick; James A Spudich
Myosin structure and function in cell motility.
Annual review of cell biology 1987;3():379-421.
1986: H M Warrick; A De Lozanne; L A Leinwand; James A Spudich
Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(24):9433-7.