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Wesley Stites

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TOP 3
Christopher C Saunders; Wesley E Stites
An electrophoretic mobility shift assay for methionine sulfoxide in proteins.
Peregrine Bell-Upp; Janine Lin; Aaron C Robinson; Wesley E Stites; Erika L Wheeler; Steven T Whitten; Bertrand García-Moreno E
Thermodynamic principles for the engineering of pH-driven conformational switches and acid insensitive proteins.
Yun Ho Kim; Wesley E Stites
Effects of excluded volume upon protein stability in covalently cross-linked proteins with variable linker lengths.

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All Publications

2012: Christopher C Saunders; Wesley E Stites
An electrophoretic mobility shift assay for methionine sulfoxide in proteins.
Analytical biochemistry 2012;421(2):767-9.
2011: Peregrine Bell-Upp; Janine Lin; Aaron C Robinson; Wesley E Stites; Erika L Wheeler; Steven T Whitten; Bertrand García-Moreno E
Thermodynamic principles for the engineering of pH-driven conformational switches and acid insensitive proteins.
Biophysical chemistry 2011;159(1):217-26.
2008: Yun Ho Kim; Wesley E Stites
Effects of excluded volume upon protein stability in covalently cross-linked proteins with variable linker lengths.
Biochemistry 2008;47(33):8804-14.
2008: Deepika Talla Singh; Wesley E Stites
Refinement of noncalorimetric determination of the change in heat capacity, DeltaC(p), of protein unfolding and validation across a wide temperature range.
Proteins 2008;71(4):1607-16.
2008: Christopher C Ross; Stewart L MacLeod; Jason R Plaxco; Jeffrey W Froude; Louis M Fink; Junru Wang; Wesley E Stites; Martin Hauer-Jensen
Inactivation of thrombomodulin by ionizing radiation in a cell-free system: possible implications for radiation responses in vascular endothelium.
Radiation research 2008;169(4):408-16.
2007: Daniel A Karp; Apostolos G Gittis; Mary R Stahley; Carolyn Fitch; Wesley E Stites; Bertrand García-Moreno E
High apparent dielectric constant inside a protein reflects structural reorganization coupled to the ionization of an internal Asp.
Biophysical journal 2007;92(6):2041-53.
2007: Michael P Byrne; Wesley E Stites
Thermal denaturations of staphylococcal nuclease wild-type and mutants monitored by fluorescence and circular dichroism are similar: lack of evidence for other than a two state thermal denaturation.
Biophysical chemistry 2007;125(2-3):490-6.
2007: Wesley E Stites; Jeffrey W Froude
Does the oxidation of methionine in thrombomodulin contribute to the hypercoaguable state of smokers and diabetics?
Medical hypotheses 2007;68(4):811-21.
2004: Junmei Chen; Wesley E Stites
Replacement of staphylococcal nuclease hydrophobic core residues with those from thermophilic homologues indicates packing is improved in some thermostable proteins.
Journal of molecular biology 2004;344(1):271-80.
2004: Y H Kim; Wesley E Stites
Oxidation of buried cysteines is slow and an insignificant factor in the structural destabilization of staphylococcal nuclease caused by H2O2 exposure.
Amino acids 2004;27(2):175-81.
2004: Junmei Chen; Zhiqiang Lu; Joshua Sakon; Wesley E Stites
Proteins with simplified hydrophobic cores compared to other packing mutants.
Biophysical chemistry 2004;110(3):239-48.
2003: Jeffery M Schwehm; Carolyn Fitch; Bao N Dang; Bertrand García-Moreno E; Wesley E Stites
Changes in stability upon charge reversal and neutralization substitution in staphylococcal nuclease are dominated by favorable electrostatic effects.
Biochemistry 2003;42(4):1118-28.
2002: Carolyn Fitch; Daniel A Karp; Kelly K Lee; Wesley E Stites; Eaton E Lattman; Bertrand García-Moreno E
Experimental pK(a) values of buried residues: analysis with continuum methods and role of water penetration.
Biophysical journal 2002;82(6):3289-304.
2001: Junghuei Chen; Wesley E Stites
Packing is a key selection factor in the evolution of protein hydrophobic cores.
Biochemistry 2001;40(50):15280-9.
2001: Junghuei Chen; Wesley E Stites
Higher-order packing interactions in triple and quadruple mutants of staphylococcal nuclease.
Biochemistry 2001;40(46):14012-9.
2001: Junghuei Chen; Wesley E Stites
Energetics of side chain packing in staphylococcal nuclease assessed by systematic double mutant cycles.
Biochemistry 2001;40(46):14004-11.
2001: J B Holder; A F Bennett; Junghuei Chen; DS Spencer; M P Byrne; Wesley E Stites
Energetics of side chain packing in staphylococcal nuclease assessed by exchange of valines, isoleucines, and leucines.
Biochemistry 2001;40(46):13998-4003.
2001: Y H Kim; A H Berry; DS Spencer; Wesley E Stites
Comparing the effect on protein stability of methionine oxidation versus mutagenesis: steps toward engineering oxidative resistance in proteins.
Protein engineering 2001;14(5):343-7.
2000: Junghuei Chen; Z Lu; Joshua Sakon; Wesley E Stites
Increasing the thermostability of staphylococcal nuclease: implications for the origin of protein thermostability.
Journal of molecular biology 2000;303(2):125-30.
2000: J J Dwyer; Apostolos G Gittis; Daniel A Karp; Eaton E Lattman; DS Spencer; Wesley E Stites; Bertrand García-Moreno E
High apparent dielectric constants in the interior of a protein reflect water penetration.
Biophysical journal 2000;79(3):1610-20.
1998: Jeffery M Schwehm; E S Kristyanne; C C Biggers; Wesley E Stites
Stability effects of increasing the hydrophobicity of solvent-exposed side chains in staphylococcal nuclease.
Biochemistry 1998;37(19):6939-48.
1998: Jeffery M Schwehm; Wesley E Stites
Application of automated methods for determination of protein conformational stability.
Methods in enzymology 1998;295():150-70.
1997: B García-Moreno; J J Dwyer; Apostolos G Gittis; Eaton E Lattman; DS Spencer; Wesley E Stites
Experimental measurement of the effective dielectric in the hydrophobic core of a protein.
Biophysical chemistry 1997;64(1-3):211-24.
1996: D S Spencer; Wesley E Stites
The M32L substitution of staphylococcal nuclease: disagreement between theoretical prediction and experimental protein stability.
Journal of molecular biology 1996;257(3):497-9.
1996: M P Byrne; C A Broomfield; Wesley E Stites
Mustard gas crosslinking of proteins through preferential alkylation of cysteines.
Journal of protein chemistry 1996;15(2):131-6.
1995: M P Byrne; Wesley E Stites
Chemically crosslinked protein dimers: stability and denaturation effects.
Protein science : a publication of the Protein Society 1995;4(12):2545-58.
1995: M P Byrne; R L Manuel; L G Lowe; Wesley E Stites
Energetic contribution of side chain hydrogen bonding to the stability of staphylococcal nuclease.
Biochemistry 1995;34(42):13949-60.
1995: Wesley E Stites; J Pranata
Empirical evaluation of the influence of side chains on the conformational entropy of the polypeptide backbone.
Proteins 1995;22(2):132-40.
1995: Wesley E Stites; M P Byrne; J Aviv; M Kaplan; P M Curtis
Instrumentation for automated determination of protein stability.
Analytical biochemistry 1995;227(1):112-22.
1994: Wesley E Stites; Alan K Meeker; David Shortle
Evidence for strained interactions between side-chains and the polypeptide backbone.
Journal of molecular biology 1994;235(1):27-32.
1993: Alan K Meeker; Y K Li; David Shortle; Wesley E Stites
A simplified protocol for isolation and characterization of ssM13 DNA templates for use in dideoxy sequencing.
BioTechniques 1993;15(3):370-2.
1993: Apostolos G Gittis; Wesley E Stites; Eaton E Lattman
The phase transition between a compact denatured state and a random coil state in staphylococcal nuclease is first-order.
Journal of molecular biology 1993;232(3):718-24.
1992: E James; P G Wu; Wesley E Stites; Ludwig Brand
Compact denatured state of a staphylococcal nuclease mutant by guanidinium as determined by resonance energy transfer.
Biochemistry 1992;31(42):10217-25.
1991: Wesley E Stites; Apostolos G Gittis; Eaton E Lattman; David Shortle
In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core.
Journal of molecular biology 1991;221(1):7-14.
1990: David Shortle; Wesley E Stites; Alan K Meeker
Contributions of the large hydrophobic amino acids to the stability of staphylococcal nuclease.
Biochemistry 1990;29(35):8033-41.